Luke Gamon
@lgamon.bsky.social
All things teammassspec/realtimechem. Survives on only the fanciest coffee and beer.
Laser capture microdissection | spatial proteomics | protein modification | atherosclerosis | inflammation and oxidation
Laser capture microdissection | spatial proteomics | protein modification | atherosclerosis | inflammation and oxidation
Pinned
Luke Gamon
@lgamon.bsky.social
· Jun 23
www.biorxiv.org
Pumped to finally show off our spatial proteomics work presented a few weeks ago at the EAS Congress @eassociety.bsky.social and now live on @biorxivpreprint.bsky.social 🥳
Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵
www.biorxiv.org/content/10.1...
Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵
www.biorxiv.org/content/10.1...
I have now officially been at KU for longer than my previous longest running job (8.5yrs, which I started at age 16). Raised through the ranks from cleaning popcorn and melted ice cream under cinema seats - all the way to projectionist and duty manager.
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Who knows… could be here another 8.5? 🥼🧪🔬🧫
November 23, 2025 at 8:43 PM
I have now officially been at KU for longer than my previous longest running job (8.5yrs, which I started at age 16). Raised through the ranks from cleaning popcorn and melted ice cream under cinema seats - all the way to projectionist and duty manager.
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
November 20, 2025 at 5:09 PM
Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Reposted by Luke Gamon
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 10:27 AM
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Reposted by Luke Gamon
Check out this chemoproteomics tour de force from @stephanhacker2.bsky.social and colleagues — want to know which electrophiles label which amino acid residues proteome-wide? Finally, a comprehensive comparative study with a ton of insights on old and new warheads and optimized workflows. Bravo!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 2:42 PM
Check out this chemoproteomics tour de force from @stephanhacker2.bsky.social and colleagues — want to know which electrophiles label which amino acid residues proteome-wide? Finally, a comprehensive comparative study with a ton of insights on old and new warheads and optimized workflows. Bravo!
Wow. This is a new one for me.
Reviewer 1:
‘Laser capture microdissection leads to a substantial loss and cautheristion of tissue and proteins.’
You’d think citing the top spatial + DVP papers, as well as our own preprint should have been enough for project feasibility 🤦♂️
Reviewer 1:
‘Laser capture microdissection leads to a substantial loss and cautheristion of tissue and proteins.’
You’d think citing the top spatial + DVP papers, as well as our own preprint should have been enough for project feasibility 🤦♂️
October 30, 2025 at 1:16 PM
Wow. This is a new one for me.
Reviewer 1:
‘Laser capture microdissection leads to a substantial loss and cautheristion of tissue and proteins.’
You’d think citing the top spatial + DVP papers, as well as our own preprint should have been enough for project feasibility 🤦♂️
Reviewer 1:
‘Laser capture microdissection leads to a substantial loss and cautheristion of tissue and proteins.’
You’d think citing the top spatial + DVP papers, as well as our own preprint should have been enough for project feasibility 🤦♂️
Does anyone know how DIA-NN handles isomers? Eg phospho would be the classic example where you have +1phos but could be on two different residues giving peptides with the same m/z but different retention time and fragmentation pattern. What if you don’t have MSMS to separate them?
October 25, 2025 at 4:26 PM
Does anyone know how DIA-NN handles isomers? Eg phospho would be the classic example where you have +1phos but could be on two different residues giving peptides with the same m/z but different retention time and fragmentation pattern. What if you don’t have MSMS to separate them?
Can someone please explain to me when it’s ok to use nominal p-values in a discovery proteomics experiment? Proteomics bluesky tells me this is absolutely against the rules but… Nature Medicine?
I mean fine if you use nominal then validate with targeted MS or something. They don’t even do that 🤷♂️
I mean fine if you use nominal then validate with targeted MS or something. They don’t even do that 🤷♂️
Several years before onset of symptoms of multiple sclerosis, there is evidence of central nervous system myelin injury
nature.com/articles/s41...
nature.com/articles/s41...
Myelin injury precedes axonal injury and symptomatic onset in multiple sclerosis - Nature Medicine
Myelin damage in multiple sclerosis can be detected up to 7 years before symptoms, with early immune pathway activation and a 21-protein panel showing promise for presymptomatic diagnosis.
nature.com
October 21, 2025 at 6:32 PM
Can someone please explain to me when it’s ok to use nominal p-values in a discovery proteomics experiment? Proteomics bluesky tells me this is absolutely against the rules but… Nature Medicine?
I mean fine if you use nominal then validate with targeted MS or something. They don’t even do that 🤷♂️
I mean fine if you use nominal then validate with targeted MS or something. They don’t even do that 🤷♂️
Reposted by Luke Gamon
Struggling to compare Bruker timsTOF methods or extract window schemes from raw .d files? I developed a free desktop tool to solve this: timsCompare! 🚀
It automates the manual work of extracting method parameters, presenting them in a structured and comparable view.
(A thread 🧵)
It automates the manual work of extracting method parameters, presenting them in a structured and comparable view.
(A thread 🧵)
October 15, 2025 at 11:01 AM
Struggling to compare Bruker timsTOF methods or extract window schemes from raw .d files? I developed a free desktop tool to solve this: timsCompare! 🚀
It automates the manual work of extracting method parameters, presenting them in a structured and comparable view.
(A thread 🧵)
It automates the manual work of extracting method parameters, presenting them in a structured and comparable view.
(A thread 🧵)
Reposted by Luke Gamon
Highly recommended PhD position in Copenhagen! Apply now!
I have a fully funded PhD position available in my group in Copenhagen: candidate.hr-manager.net/ApplicationI...
PhD fellowship in Organic Synthesis and Supramolecular Chemistry
The Department of Chemistry at the University of Copenhagen invites applicants for a PhD fellowship in Organic chemistry with a particular focus on supramolecul
candidate.hr-manager.net
October 10, 2025 at 8:24 AM
Highly recommended PhD position in Copenhagen! Apply now!
Reposted by Luke Gamon
Reposting this.
If you are interested in applying for this fellowship in a research area of our lab's interest, let me know.
If you are interested in applying for this fellowship in a research area of our lab's interest, let me know.
Three Bicentenary research fellowships on offer at @officialuom.bsky.social @fbmh-uom.bsky.social for exceptional ECRs with <3y postdoc experience
3yrs salary + £30k pa research expenses
Do you have a great idea & want to join @mermanchester.bsky.social?
www.bmh.manchester.ac.uk/research/sup...
3yrs salary + £30k pa research expenses
Do you have a great idea & want to join @mermanchester.bsky.social?
www.bmh.manchester.ac.uk/research/sup...
Bicentenary Early Career Research Fellowships | Biology, Medicine and Health | University of Manchester
Find out about and apply for a Bicentenary Early Career Research Fellowship.
www.bmh.manchester.ac.uk
October 10, 2025 at 8:36 AM
Reposting this.
If you are interested in applying for this fellowship in a research area of our lab's interest, let me know.
If you are interested in applying for this fellowship in a research area of our lab's interest, let me know.
Reposted by Luke Gamon
Do you have an exciting experiment that you'd love to do using proteomics in the context of your work? YPIC funds 5000 eur for your research idea to come to life!
⬇️⬇️⬇️⬇️⬇️
⬇️⬇️⬇️⬇️⬇️
🎉 Exciting opportunity for students in proteomics! 🎉
The Student Proteomics Fund (SPF) is back!
📅 Key dates:
Call opens: October 1, 2025
Submission deadline: November 30, 2025
Don’t miss your chance to bring your research idea to life!
New boost
#SPF #YPIC #Proteomics
The Student Proteomics Fund (SPF) is back!
📅 Key dates:
Call opens: October 1, 2025
Submission deadline: November 30, 2025
Don’t miss your chance to bring your research idea to life!
New boost
#SPF #YPIC #Proteomics
October 6, 2025 at 6:08 AM
Do you have an exciting experiment that you'd love to do using proteomics in the context of your work? YPIC funds 5000 eur for your research idea to come to life!
⬇️⬇️⬇️⬇️⬇️
⬇️⬇️⬇️⬇️⬇️
Reposted by Luke Gamon
Finally, we're looking for someone to join our #MassSpec Core Facility to support all the protein/peptide MS & development needs of our research groups utilising:
🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis
More at:
www.cruk.manchester.ac.uk/career/mi-25...
🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis
More at:
www.cruk.manchester.ac.uk/career/mi-25...
Mass Spec
xkcd.com
October 3, 2025 at 2:17 PM
Finally, we're looking for someone to join our #MassSpec Core Facility to support all the protein/peptide MS & development needs of our research groups utilising:
🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis
More at:
www.cruk.manchester.ac.uk/career/mi-25...
🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis
More at:
www.cruk.manchester.ac.uk/career/mi-25...
Join a department that gives you surprise slushies for the yearly department seminar day.
October 2, 2025 at 4:46 PM
Join a department that gives you surprise slushies for the yearly department seminar day.
4th grant application of the year sent out into the ether. Number 5 will leave the coop next week.🤞
October 1, 2025 at 3:01 PM
4th grant application of the year sent out into the ether. Number 5 will leave the coop next week.🤞
Reposted by Luke Gamon
Come work with me. Excellent startup package. su.varbi.com/en/what:job/...
Assistant Professor in Data-driven cell and molecular biology
At Stockholm University the Department of Biochemistry and Biophysics (DBB) and the Department of Molecular Biosciences, The Wenner-Gren Institute (MBW) are looking to fill one position as DDLS fellow
su.varbi.com
September 30, 2025 at 7:43 AM
Come work with me. Excellent startup package. su.varbi.com/en/what:job/...
I’m seeing some chatter about a new centralised ID system in the UK.
As someone who lives in a country where basically your entire identity is digitally linked to a single ID I have mixed feelings but damnnnnn is life smooth when everything is integrated.
As someone who lives in a country where basically your entire identity is digitally linked to a single ID I have mixed feelings but damnnnnn is life smooth when everything is integrated.
September 27, 2025 at 11:06 AM
I’m seeing some chatter about a new centralised ID system in the UK.
As someone who lives in a country where basically your entire identity is digitally linked to a single ID I have mixed feelings but damnnnnn is life smooth when everything is integrated.
As someone who lives in a country where basically your entire identity is digitally linked to a single ID I have mixed feelings but damnnnnn is life smooth when everything is integrated.
Reposted by Luke Gamon
Proteomics Webinar: DIA with FragPipe, DIA-NN, and Skyline
Presenters: Eduard Sabidó and Brendan MacLean
When: Tuesday, September 16, 8am (Pacific Time)
Register Now ... skyline.ms/project/home...
#massspec #proteomics
Presenters: Eduard Sabidó and Brendan MacLean
When: Tuesday, September 16, 8am (Pacific Time)
Register Now ... skyline.ms/project/home...
#massspec #proteomics
Start Page: /home/software/Skyline/events/2025 Webinars/Webinar 26
skyline.ms
September 15, 2025 at 8:26 AM
Proteomics Webinar: DIA with FragPipe, DIA-NN, and Skyline
Presenters: Eduard Sabidó and Brendan MacLean
When: Tuesday, September 16, 8am (Pacific Time)
Register Now ... skyline.ms/project/home...
#massspec #proteomics
Presenters: Eduard Sabidó and Brendan MacLean
When: Tuesday, September 16, 8am (Pacific Time)
Register Now ... skyline.ms/project/home...
#massspec #proteomics
Our new spatial proteomics workflow is a game changer. Prepped 40 samples on Friday in 6hrs all the way from tissue on a slide to peptides in the autosampler.
Almost reminds me of my organic chem PhD days when you could think up an experiment in the morning and have the result by the afternoon.
Almost reminds me of my organic chem PhD days when you could think up an experiment in the morning and have the result by the afternoon.
September 13, 2025 at 4:23 PM
Our new spatial proteomics workflow is a game changer. Prepped 40 samples on Friday in 6hrs all the way from tissue on a slide to peptides in the autosampler.
Almost reminds me of my organic chem PhD days when you could think up an experiment in the morning and have the result by the afternoon.
Almost reminds me of my organic chem PhD days when you could think up an experiment in the morning and have the result by the afternoon.
A little early morning science with the 2.5yo. Always been obsessed with gloves for some reason? Probably even better with PPE than I am
September 12, 2025 at 7:00 AM
A little early morning science with the 2.5yo. Always been obsessed with gloves for some reason? Probably even better with PPE than I am
Reposted by Luke Gamon
@cnicproteomics.bsky.social is finally in Bluesky!!
Follow us to learn about our quest to advance proteomics research with a special focus on open search strategies and the study of post-translational modifications (PTMs).
Follow us to learn about our quest to advance proteomics research with a special focus on open search strategies and the study of post-translational modifications (PTMs).
September 11, 2025 at 1:55 PM
@cnicproteomics.bsky.social is finally in Bluesky!!
Follow us to learn about our quest to advance proteomics research with a special focus on open search strategies and the study of post-translational modifications (PTMs).
Follow us to learn about our quest to advance proteomics research with a special focus on open search strategies and the study of post-translational modifications (PTMs).
Reposted by Luke Gamon
📢 My lab has a 2.5-year Postdoc position available!
If you're interested in smooth muscle cell biology in atherosclerosis, then check out the Postdoc call here:
fa-eosd-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...
#postdoc #research #hiring #atherosclerosis
If you're interested in smooth muscle cell biology in atherosclerosis, then check out the Postdoc call here:
fa-eosd-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...
#postdoc #research #hiring #atherosclerosis
September 6, 2025 at 4:40 PM
📢 My lab has a 2.5-year Postdoc position available!
If you're interested in smooth muscle cell biology in atherosclerosis, then check out the Postdoc call here:
fa-eosd-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...
#postdoc #research #hiring #atherosclerosis
If you're interested in smooth muscle cell biology in atherosclerosis, then check out the Postdoc call here:
fa-eosd-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...
#postdoc #research #hiring #atherosclerosis
Reposted by Luke Gamon
Do you or your lab use the Skyline software for #MassSpectrometry #proteomics? I'm looking for instructors to help with this year's Skyline Online, a virtual workshop/crash-course for all things Skyline! I'm especially looking for early career researchers for this opportunity. Please DM!
September 6, 2025 at 5:11 PM
Do you or your lab use the Skyline software for #MassSpectrometry #proteomics? I'm looking for instructors to help with this year's Skyline Online, a virtual workshop/crash-course for all things Skyline! I'm especially looking for early career researchers for this opportunity. Please DM!
Reposted by Luke Gamon
Scalable Acid-Aided Lysis of Skin Samples Improves Proteome Coverage www.sciencedirect.co...
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#proteomics #prot-paper
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#proteomics #prot-paper
September 7, 2025 at 9:20 AM
Scalable Acid-Aided Lysis of Skin Samples Improves Proteome Coverage www.sciencedirect.co...
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#proteomics #prot-paper
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#proteomics #prot-paper
Reposted by Luke Gamon
Any glyco people want to guess why pure TFA added to ECM samples and held at 60 degrees celsius for 30 min might improve proteome coverage?
Scalable Acid-Aided Lysis of Skin Samples Improves Proteome Coverage www.sciencedirect.co...
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#proteomics #prot-paper
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#proteomics #prot-paper
September 7, 2025 at 10:14 AM
Any glyco people want to guess why pure TFA added to ECM samples and held at 60 degrees celsius for 30 min might improve proteome coverage?
Seriously pumped for today! Deep Visual Proteomics masterclass here we come 🤓
August 25, 2025 at 7:07 AM
Seriously pumped for today! Deep Visual Proteomics masterclass here we come 🤓