Luke Gamon
@lgamon.bsky.social
All things teammassspec/realtimechem. Survives on only the fanciest coffee and beer.
Laser capture microdissection | spatial proteomics | protein modification | atherosclerosis | inflammation and oxidation
Laser capture microdissection | spatial proteomics | protein modification | atherosclerosis | inflammation and oxidation
I have now officially been at KU for longer than my previous longest running job (8.5yrs, which I started at age 16). Raised through the ranks from cleaning popcorn and melted ice cream under cinema seats - all the way to projectionist and duty manager.
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Who knows… could be here another 8.5? 🥼🧪🔬🧫
November 23, 2025 at 8:43 PM
I have now officially been at KU for longer than my previous longest running job (8.5yrs, which I started at age 16). Raised through the ranks from cleaning popcorn and melted ice cream under cinema seats - all the way to projectionist and duty manager.
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Who knows… could be here another 8.5? 🥼🧪🔬🧫
Join a department that gives you surprise slushies for the yearly department seminar day.
October 2, 2025 at 4:46 PM
Join a department that gives you surprise slushies for the yearly department seminar day.
A little early morning science with the 2.5yo. Always been obsessed with gloves for some reason? Probably even better with PPE than I am
September 12, 2025 at 7:00 AM
A little early morning science with the 2.5yo. Always been obsessed with gloves for some reason? Probably even better with PPE than I am
Seriously pumped for today! Deep Visual Proteomics masterclass here we come 🤓
August 25, 2025 at 7:07 AM
Seriously pumped for today! Deep Visual Proteomics masterclass here we come 🤓
Picked up this little bad boy for £6k that I’m now calling ‘Her Majesty’s LC’ (purchased secondhand from the UK, was in its prime during the previous monarch’s reign)
And… it’s alive! Say goodbye to LC downtime… just swap in a spare!
And… it’s alive! Say goodbye to LC downtime… just swap in a spare!
August 15, 2025 at 5:39 PM
Picked up this little bad boy for £6k that I’m now calling ‘Her Majesty’s LC’ (purchased secondhand from the UK, was in its prime during the previous monarch’s reign)
And… it’s alive! Say goodbye to LC downtime… just swap in a spare!
And… it’s alive! Say goodbye to LC downtime… just swap in a spare!
This paper is just the first part of our journey into the wonders of spatial biology. So many cool things I hope to share this year. Stay tuned.
P.S. Here's a bonus video because laser capture is cool (stay to the end to watch the square hit the hanging drop and float away 🧐)
P.S. Here's a bonus video because laser capture is cool (stay to the end to watch the square hit the hanging drop and float away 🧐)
June 23, 2025 at 6:56 PM
This paper is just the first part of our journey into the wonders of spatial biology. So many cool things I hope to share this year. Stay tuned.
P.S. Here's a bonus video because laser capture is cool (stay to the end to watch the square hit the hanging drop and float away 🧐)
P.S. Here's a bonus video because laser capture is cool (stay to the end to watch the square hit the hanging drop and float away 🧐)
If you made it this far you get the gold nugget - 96-well style capture via flat optically clear PCR-cap stripes (8caps*12strips=96) coupled with 96-well format sample processing and stage-tip cleanup. What I call the poor-mans ProteoCHIP. Easily collect + process >200 samples per day.
June 23, 2025 at 6:56 PM
If you made it this far you get the gold nugget - 96-well style capture via flat optically clear PCR-cap stripes (8caps*12strips=96) coupled with 96-well format sample processing and stage-tip cleanup. What I call the poor-mans ProteoCHIP. Easily collect + process >200 samples per day.
Finally, looking at a complex human plaque we saw very large differences based on gross morphology (e.g. smooth muscle media vs haemorrhaged necrotic core) and immune-cell mediated matrix remodeling in the developing shoulder (one of the areas known to be prone to rupture).
June 23, 2025 at 6:56 PM
Finally, looking at a complex human plaque we saw very large differences based on gross morphology (e.g. smooth muscle media vs haemorrhaged necrotic core) and immune-cell mediated matrix remodeling in the developing shoulder (one of the areas known to be prone to rupture).
Going back to human plaques we were able to show dramatic proteome changes over ~200µm distances at the vessel wall (lumen = where blood normally flows through the artery) and a nice gradient of proteins such as apolipoproteins and collagens.
June 23, 2025 at 6:56 PM
Going back to human plaques we were able to show dramatic proteome changes over ~200µm distances at the vessel wall (lumen = where blood normally flows through the artery) and a nice gradient of proteins such as apolipoproteins and collagens.
In retrospect starting with such a complex beast wasn't the best idea... which is partly why we validated the method in more homogenous mouse heart tissue. Our laser captured myocardium (heart muscle) looked a lot like single cell cardiomyocytes (heart muscle cells) 👍 H/T @simionkreimer.bsky.social
June 23, 2025 at 6:56 PM
In retrospect starting with such a complex beast wasn't the best idea... which is partly why we validated the method in more homogenous mouse heart tissue. Our laser captured myocardium (heart muscle) looked a lot like single cell cardiomyocytes (heart muscle cells) 👍 H/T @simionkreimer.bsky.social
Atherosclerotic plaques are the blockages in your arteries that cause heart attacks and stroke and they are CRAZY complex. Not only very different from person to person but also very different even within a single plaque (e.g. very fatty, fibrous, inflamed, calcified etc).
June 23, 2025 at 6:56 PM
Atherosclerotic plaques are the blockages in your arteries that cause heart attacks and stroke and they are CRAZY complex. Not only very different from person to person but also very different even within a single plaque (e.g. very fatty, fibrous, inflamed, calcified etc).
Here we present an accessible workflow for laser capture microdissection based spatial proteomics (similar to work by legends @erwinschoof.bsky.social, @coscialab.bsky.social, @andreasmund.bsky.social etc) and apply it in the context of human atherosclerosis.
June 23, 2025 at 6:56 PM
Here we present an accessible workflow for laser capture microdissection based spatial proteomics (similar to work by legends @erwinschoof.bsky.social, @coscialab.bsky.social, @andreasmund.bsky.social etc) and apply it in the context of human atherosclerosis.
Wow check out these astrocytes stretching into stroke infarcted mouse brain 🤩
Sometimes I wonder if I came to microscopy too late in life.
Sometimes I wonder if I came to microscopy too late in life.
May 20, 2025 at 4:49 PM
Wow check out these astrocytes stretching into stroke infarcted mouse brain 🤩
Sometimes I wonder if I came to microscopy too late in life.
Sometimes I wonder if I came to microscopy too late in life.
Feels good to be back in the lab playing with tissue sections again. Also, kinda beautiful? 🐭 🧠
May 8, 2025 at 4:52 PM
Feels good to be back in the lab playing with tissue sections again. Also, kinda beautiful? 🐭 🧠
The first fluorescence scan from my project codenamed MOSAIC. So bad, yet so beautiful. At least matches its namesake.
Who knew you could auto-expose each tile in a tissue scan 🤷♂️ #FluorescenceFriday
Who knew you could auto-expose each tile in a tissue scan 🤷♂️ #FluorescenceFriday
April 11, 2025 at 11:11 AM
The first fluorescence scan from my project codenamed MOSAIC. So bad, yet so beautiful. At least matches its namesake.
Who knew you could auto-expose each tile in a tissue scan 🤷♂️ #FluorescenceFriday
Who knew you could auto-expose each tile in a tissue scan 🤷♂️ #FluorescenceFriday
Finally got around to playing with AlphaQuant from @mannlab.bsky.social. Works basically straight out of the box, also for proteoforms! A lot of chaff and validation necessary but here’s a nice differential proteoform of Progranulin being cleaved to release Granulin-E.
March 28, 2025 at 9:57 AM
Finally got around to playing with AlphaQuant from @mannlab.bsky.social. Works basically straight out of the box, also for proteoforms! A lot of chaff and validation necessary but here’s a nice differential proteoform of Progranulin being cleaved to release Granulin-E.
My phone is dying. Send help. Seriously I keep deleting like 1GB from the storage every few days and then it’s immediately ‘full’ again.
March 28, 2025 at 7:32 AM
My phone is dying. Send help. Seriously I keep deleting like 1GB from the storage every few days and then it’s immediately ‘full’ again.
Perfect morning and a perfect view to kick off the day for our weekly proteomics meeting ☀️ ⛅️ 🏙️
March 20, 2025 at 7:31 AM
Perfect morning and a perfect view to kick off the day for our weekly proteomics meeting ☀️ ⛅️ 🏙️
Randomly decided to book out a room for the day in the top of ‘the tower’ for a little mini group hackathon since so many of us are working in R now. Definitely gonna do this again. At the very least a nice change of scenery ⛅️ #Proteomics #TeamMassSpec
March 13, 2025 at 2:35 PM
Randomly decided to book out a room for the day in the top of ‘the tower’ for a little mini group hackathon since so many of us are working in R now. Definitely gonna do this again. At the very least a nice change of scenery ⛅️ #Proteomics #TeamMassSpec
And the streptavidin ‘peak’ in the viewer 😅
March 11, 2025 at 6:02 AM
And the streptavidin ‘peak’ in the viewer 😅
As much as a dislike the new ‘1 page per sample’ DIA-NN report pdf, it is super informative. If you’ve ever wondered what signal suppression looks like, here’s some chunky streptavidin peptides at 3/5mins causing havoc. #TeamMassSpec
March 11, 2025 at 5:50 AM
As much as a dislike the new ‘1 page per sample’ DIA-NN report pdf, it is super informative. If you’ve ever wondered what signal suppression looks like, here’s some chunky streptavidin peptides at 3/5mins causing havoc. #TeamMassSpec
Always a nice surprise to arrive to your desk like this 🎂 🇩🇰
February 12, 2025 at 10:48 AM
Always a nice surprise to arrive to your desk like this 🎂 🇩🇰
Even Gemini is trolling me!
Cites our 2022 paper, then ignores us and lists only the authors of the new paper as working in the field 🫠
Cites our 2022 paper, then ignores us and lists only the authors of the new paper as working in the field 🫠
February 6, 2025 at 6:42 PM
Even Gemini is trolling me!
Cites our 2022 paper, then ignores us and lists only the authors of the new paper as working in the field 🫠
Cites our 2022 paper, then ignores us and lists only the authors of the new paper as working in the field 🫠