Stephan Hacker
@stephanhacker2.bsky.social
Assistant Professor at Leiden University. Covalent inhibitors and chemoproteomics for antibiotics. Views my own. he/him/his. https://orcid.org/0000-0001-5420-4824
Reposted by Stephan Hacker
A pipeline for proteome-wide analysis of electrophile selectivity www.nature.com/artic...
---
#proteomics #prot-paper
---
#proteomics #prot-paper
November 2, 2025 at 6:40 PM
A pipeline for proteome-wide analysis of electrophile selectivity www.nature.com/artic...
---
#proteomics #prot-paper
---
#proteomics #prot-paper
Reposted by Stephan Hacker
Reposted by Stephan Hacker
Do you want to help to gather data on the tenure- and career-track systems at Dutch Universities?
Our friends at NextGenPhys@NL, the network of emerging physics researchers, are conducting a survey to explore how tenure- and career-track systems are structured.(1/4)
docs.google.com/forms/d/e/1F...
Our friends at NextGenPhys@NL, the network of emerging physics researchers, are conducting a survey to explore how tenure- and career-track systems are structured.(1/4)
docs.google.com/forms/d/e/1F...
Tenure-track/career-track systems at Universities in the Netherlands
We, the NextGenPhys@NL board, aim to collect information about the different types of tenure-track/career-track systems currently existing at Universities across the Netherlands. The scope of this for...
docs.google.com
November 10, 2025 at 1:07 PM
Do you want to help to gather data on the tenure- and career-track systems at Dutch Universities?
Our friends at NextGenPhys@NL, the network of emerging physics researchers, are conducting a survey to explore how tenure- and career-track systems are structured.(1/4)
docs.google.com/forms/d/e/1F...
Our friends at NextGenPhys@NL, the network of emerging physics researchers, are conducting a survey to explore how tenure- and career-track systems are structured.(1/4)
docs.google.com/forms/d/e/1F...
Interesting talk by Petra de Jongh at the #Reedijk Symposium. She talked about the use of in situ/operando electron microscopy to better understand heterogeneous catalysts under conditions that are close to those used in industry-scale reactors.
#ChemSky #Catalysis
#ChemSky #Catalysis
November 7, 2025 at 3:28 PM
Interesting talk by Petra de Jongh at the #Reedijk Symposium. She talked about the use of in situ/operando electron microscopy to better understand heterogeneous catalysts under conditions that are close to those used in industry-scale reactors.
#ChemSky #Catalysis
#ChemSky #Catalysis
Very happy that Storm van der Voort - PhD in the group of Hermen Overkleeft and in our group - was selected for a talk at the #Reedijk Symposium. He gave an excellent presentation on using direct-to-biology to efficiently make covalent libraries for antibacterial screening
#ChemSky #ChemBio #ProudPI
#ChemSky #ChemBio #ProudPI
November 7, 2025 at 1:42 PM
Great talk by Jaco Geuchies at the #Reedijk Symposium of the Leiden Institute of Chemistry. He told us, how his group uses ultrafast spectroscopy to better understand the structure and properties of soft semiconductor materials such as metal-halide #perovskites. pubs.acs.org/doi/full/10....
#ChemSky
#ChemSky
November 7, 2025 at 1:30 PM
Great talk by Jaco Geuchies at the #Reedijk Symposium of the Leiden Institute of Chemistry. He told us, how his group uses ultrafast spectroscopy to better understand the structure and properties of soft semiconductor materials such as metal-halide #perovskites. pubs.acs.org/doi/full/10....
#ChemSky
#ChemSky
Insightful talk by Jeroen Codee at the #Reedijk Symposium. He talked about the importance of synthetic organic chemistry to make complex glycans to better understand #glycobiology. Very impressive, systematic work on glycosylation reactions. pubs.rsc.org/en/content/a...
#ChemSky #ChemBio #Glycotime
#ChemSky #ChemBio #Glycotime
November 7, 2025 at 12:54 PM
Insightful talk by Jeroen Codee at the #Reedijk Symposium. He talked about the importance of synthetic organic chemistry to make complex glycans to better understand #glycobiology. Very impressive, systematic work on glycosylation reactions. pubs.rsc.org/en/content/a...
#ChemSky #ChemBio #Glycotime
#ChemSky #ChemBio #Glycotime
Reposted by Stephan Hacker
Jeroen Codee speaks at the #Reedijk Symposium: "A new twist in the tale - stereoselective glycosylation reactions for bacterial glycan assembly". Exciting insights into the importance of advanced glycosylation chemistries to make complex glycosides to better understand (bacterial) biology.
November 7, 2025 at 12:47 PM
Jeroen Codee speaks at the #Reedijk Symposium: "A new twist in the tale - stereoselective glycosylation reactions for bacterial glycan assembly". Exciting insights into the importance of advanced glycosylation chemistries to make complex glycosides to better understand (bacterial) biology.
Craig Crews also gave exciting insights into the power of RIPTACs that bring together a protein that is selectively expressed in tumors and a pan-essential protein. In this way, the pan-essential protein is only fully engaged in cancer cells leading to selective killing. www.cell.com/cell-chemica...
November 7, 2025 at 10:51 AM
Craig Crews also gave exciting insights into the power of RIPTACs that bring together a protein that is selectively expressed in tumors and a pan-essential protein. In this way, the pan-essential protein is only fully engaged in cancer cells leading to selective killing. www.cell.com/cell-chemica...
Inspiring #Havinga Medal lecture by @craigmcrews.bsky.social at the #Reedijk Symposium. Amazing to hear him talk about the full story of the development of induced proximity into a new paradigm of event-driven pharmacology for #DrugDiscovery.
www.nature.com/articles/s41...
#ChemSky #ChemBio #TPD
www.nature.com/articles/s41...
#ChemSky #ChemBio #TPD
November 7, 2025 at 10:31 AM
Inspiring #Havinga Medal lecture by @craigmcrews.bsky.social at the #Reedijk Symposium. Amazing to hear him talk about the full story of the development of induced proximity into a new paradigm of event-driven pharmacology for #DrugDiscovery.
www.nature.com/articles/s41...
#ChemSky #ChemBio #TPD
www.nature.com/articles/s41...
#ChemSky #ChemBio #TPD
Reposted by Stephan Hacker
Huge congratulations to @craigmcrews.bsky.social on being awarded the #Havinga medal at the #Reedijk Symposium of the Leiden Institute of Chemistry for his groundbreaking work on induced proximity, including targeted protein degradation, as a new paradigm for #DrugDiscovery.
#ChemSky #ChemBio #TPD
#ChemSky #ChemBio #TPD
November 7, 2025 at 10:20 AM
Huge congratulations to @craigmcrews.bsky.social on being awarded the #Havinga medal at the #Reedijk Symposium of the Leiden Institute of Chemistry for his groundbreaking work on induced proximity, including targeted protein degradation, as a new paradigm for #DrugDiscovery.
#ChemSky #ChemBio #TPD
#ChemSky #ChemBio #TPD
Great talk by @kimmb1.bsky.social of our @led3hub.bsky.social at the #Reedijk Symposium. She talked about the power of activity-based probes to study protein function on the example of live cell imaging of myeloperoxidase activity to study NETosis. www.nature.com/articles/s42...
#ChemBio #ChemSky
#ChemBio #ChemSky
November 7, 2025 at 9:07 AM
Great talk by @kimmb1.bsky.social of our @led3hub.bsky.social at the #Reedijk Symposium. She talked about the power of activity-based probes to study protein function on the example of live cell imaging of myeloperoxidase activity to study NETosis. www.nature.com/articles/s42...
#ChemBio #ChemSky
#ChemBio #ChemSky
Reposted by Stephan Hacker
Kim Bonger (@kimmb1.bsky.social) represents our @led3hub.bsky.social at the #Reedijk Symposium of the Leiden Institute of Chemistry with a great talk on "Chemical tools for profiling protein activity and cell targeting in (auto)immune disease".
#ChemBio #ChemSky #DrugDiscovery #FluorescenceFriday
#ChemBio #ChemSky #DrugDiscovery #FluorescenceFriday
November 7, 2025 at 9:00 AM
Kim Bonger (@kimmb1.bsky.social) represents our @led3hub.bsky.social at the #Reedijk Symposium of the Leiden Institute of Chemistry with a great talk on "Chemical tools for profiling protein activity and cell targeting in (auto)immune disease".
#ChemBio #ChemSky #DrugDiscovery #FluorescenceFriday
#ChemBio #ChemSky #DrugDiscovery #FluorescenceFriday
Reposted by Stephan Hacker
📢 New Award for #ECRs in #Biochemistry!
Apply for our Meeting Award to get:
✅ 500 € grant for the #Biochemistry2026 conference
✅ 1 year of free division membership
Apply now: en.gdch.de/network-s...
Share with a #ECR who needs to see this! 👇
#Chemistry #ChemBio #AcademicTwitter #PhD
Apply for our Meeting Award to get:
✅ 500 € grant for the #Biochemistry2026 conference
✅ 1 year of free division membership
Apply now: en.gdch.de/network-s...
Share with a #ECR who needs to see this! 👇
#Chemistry #ChemBio #AcademicTwitter #PhD
November 4, 2025 at 4:47 PM
📢 New Award for #ECRs in #Biochemistry!
Apply for our Meeting Award to get:
✅ 500 € grant for the #Biochemistry2026 conference
✅ 1 year of free division membership
Apply now: en.gdch.de/network-s...
Share with a #ECR who needs to see this! 👇
#Chemistry #ChemBio #AcademicTwitter #PhD
Apply for our Meeting Award to get:
✅ 500 € grant for the #Biochemistry2026 conference
✅ 1 year of free division membership
Apply now: en.gdch.de/network-s...
Share with a #ECR who needs to see this! 👇
#Chemistry #ChemBio #AcademicTwitter #PhD
Reposted by Stephan Hacker
Profiling the proteome-wide selectivity of diverse electrophiles #nature #MassSpecRSS
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow. This comparative analysis and the probes described could help guide the discovery and design of covalent ligands targeting residues beyond cysteine.
dlvr.it
October 31, 2025 at 10:03 AM
Profiling the proteome-wide selectivity of diverse electrophiles #nature #MassSpecRSS
Reposted by Stephan Hacker
I am thrilled to see the paper out now and grateful to all coauthors for their contributions to this work!
Among a number of changes, we included additional probes as well as experiments in live bacterial cells. So be sure to check it out even if you are already familiar with the preprint!
Among a number of changes, we included additional probes as well as experiments in live bacterial cells. So be sure to check it out even if you are already familiar with the preprint!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 31, 2025 at 4:38 PM
I am thrilled to see the paper out now and grateful to all coauthors for their contributions to this work!
Among a number of changes, we included additional probes as well as experiments in live bacterial cells. So be sure to check it out even if you are already familiar with the preprint!
Among a number of changes, we included additional probes as well as experiments in live bacterial cells. So be sure to check it out even if you are already familiar with the preprint!
Reposted by Stephan Hacker
Great work Stephan! Of course we are happy that TMS-EBX is the reagent labelling most cysteines among all investigated! Great also to finally quantify the alkynylation to addition ratio for alkyl-EBX reagents.
October 31, 2025 at 7:25 AM
Great work Stephan! Of course we are happy that TMS-EBX is the reagent labelling most cysteines among all investigated! Great also to finally quantify the alkynylation to addition ratio for alkyl-EBX reagents.
I was very happy to host Nicolas Winssinger today for a well-attended @led3hub.bsky.social Lecture.
He gave us exciting insights into the power of nucleic acid assemblies. Very exciting data on a highly selective thrombin inhibitor that can be switched off on demand. www.nature.com/articles/s41...
He gave us exciting insights into the power of nucleic acid assemblies. Very exciting data on a highly selective thrombin inhibitor that can be switched off on demand. www.nature.com/articles/s41...
October 30, 2025 at 3:35 PM
I was very happy to host Nicolas Winssinger today for a well-attended @led3hub.bsky.social Lecture.
He gave us exciting insights into the power of nucleic acid assemblies. Very exciting data on a highly selective thrombin inhibitor that can be switched off on demand. www.nature.com/articles/s41...
He gave us exciting insights into the power of nucleic acid assemblies. Very exciting data on a highly selective thrombin inhibitor that can be switched off on demand. www.nature.com/articles/s41...
Reposted by Stephan Hacker
Wow! Looks like Christmas morning for the machine learning folks!
October 30, 2025 at 2:42 PM
Wow! Looks like Christmas morning for the machine learning folks!
Reposted by Stephan Hacker
How selective is your favourite electrophile? 🤔 🧪 🧫 🎯
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 2:39 PM
How selective is your favourite electrophile? 🤔 🧪 🧫 🎯
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Reposted by Stephan Hacker
Check out this chemoproteomics tour de force from @stephanhacker2.bsky.social and colleagues — want to know which electrophiles label which amino acid residues proteome-wide? Finally, a comprehensive comparative study with a ton of insights on old and new warheads and optimized workflows. Bravo!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 2:42 PM
Check out this chemoproteomics tour de force from @stephanhacker2.bsky.social and colleagues — want to know which electrophiles label which amino acid residues proteome-wide? Finally, a comprehensive comparative study with a ton of insights on old and new warheads and optimized workflows. Bravo!
Reposted by Stephan Hacker
This project started 5 years ago. It led us to add isotope-labeling support to #FragPipe/#IonQuant. Since then, the tools have grown so much and are now widely used in #Chemoproteomics.
Huge thanks to everyone, and special thanks to @stephanhacker2.bsky.social and @pzanon.bsky.social
Huge thanks to everyone, and special thanks to @stephanhacker2.bsky.social and @pzanon.bsky.social
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 2:15 PM
This project started 5 years ago. It led us to add isotope-labeling support to #FragPipe/#IonQuant. Since then, the tools have grown so much and are now widely used in #Chemoproteomics.
Huge thanks to everyone, and special thanks to @stephanhacker2.bsky.social and @pzanon.bsky.social
Huge thanks to everyone, and special thanks to @stephanhacker2.bsky.social and @pzanon.bsky.social
Reposted by Stephan Hacker
How can the selectivity of covalent inhibitors be studied at diverse amino acids?
In their Nature Chemistry paper the group of @stephanhacker2.bsky.social of our LED3hub developed an unbiased workflow to study electrophile reactivity and selectivity proteome-wide.(1/2)
www.nature.com/articles/s41...
In their Nature Chemistry paper the group of @stephanhacker2.bsky.social of our LED3hub developed an unbiased workflow to study electrophile reactivity and selectivity proteome-wide.(1/2)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 10:53 AM
How can the selectivity of covalent inhibitors be studied at diverse amino acids?
In their Nature Chemistry paper the group of @stephanhacker2.bsky.social of our LED3hub developed an unbiased workflow to study electrophile reactivity and selectivity proteome-wide.(1/2)
www.nature.com/articles/s41...
In their Nature Chemistry paper the group of @stephanhacker2.bsky.social of our LED3hub developed an unbiased workflow to study electrophile reactivity and selectivity proteome-wide.(1/2)
www.nature.com/articles/s41...
Our new Nature Chemistry paper is also accompanied by an insightful News and Views article by @jeyunjo.bsky.social and @mbogyo.bsky.social.
Check it out here: www.nature.com/articles/s41...
Thank you very much for the kind comments on our work!
Check it out here: www.nature.com/articles/s41...
Thank you very much for the kind comments on our work!
A pipeline for proteome-wide analysis of electrophile selectivity - Nature Chemistry
In the growing field of chemical proteomics, there is a need for general methods to map the reactivity profiles of covalent probes in complex proteomes. Now, a completely unbiased proteomic workflow h...
www.nature.com
October 30, 2025 at 10:43 AM
Our new Nature Chemistry paper is also accompanied by an insightful News and Views article by @jeyunjo.bsky.social and @mbogyo.bsky.social.
Check it out here: www.nature.com/articles/s41...
Thank you very much for the kind comments on our work!
Check it out here: www.nature.com/articles/s41...
Thank you very much for the kind comments on our work!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 10:27 AM
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...