Lakshita Sharma
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lakshitaaa.bsky.social
Lakshita Sharma
@lakshitaaa.bsky.social
180 followers 280 following 9 posts
Learning from the mistakes of others who took my advice 🤐 | Exploring Proteostasis with Mass Spectrometry 🧑‍💻🥼 | Science & curiosity in constant motion 🛣️| 📍 Germany
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Reposted by Lakshita Sharma
c0nc0rdance.bsky.social
University administration:
"We need you to complete 26 hours of lab safety training 🧪 each year so that everyone is safe and responsible."

Also university administration:
Stolen from the Internet, a sign that says "If Elevator does not move do a small jump it should move after", and a dirty-white freight elevator wall and aged elevator controls with only floors 1 and 2." I have no idea where this really is, I just stole it off Wikimedia.
October 15, 2025 at 3:09 PM
Reposted by Lakshita Sharma
liamfaller.bsky.social
Very cool stuff on the co-translational folding of dimers by Sander Tans and his team @amolf-nl.bsky.social:

www.nature.com/articles/s41...
Co-translational ribosome pairing enables native assembly of misfolding-prone subunits - Nature Communications
Protein complexes are essential for cell function. Here, authors show that paired ribosomes can help each other’s nascent chains fold correctly, enabling proper dimer assembly and preventing misfoldin...
www.nature.com
August 28, 2025 at 12:07 PM
Reposted by Lakshita Sharma
alirezamashaghi.bsky.social
We recently carried out the first single-molecule analysis of protein folding & unfolding in a cytosolic environment using optical tweezers—moving beyond simplistic buffer conditions to study proteins where they actually function.
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#ChemSky #Biophysics
Cytosolic Interactome Protects Against Protein Unfolding in a Single Molecule Experiment
Single molecule experiments aimed at probing protein folding are typically performed in buffer conditions and cannot therefore be representative of the multi-faceted and complex cooperative interacti...
advanced.onlinelibrary.wiley.com
September 11, 2025 at 5:51 AM
Reposted by Lakshita Sharma
chercheurjuteux.com
ATP plays a structural role in Hsp90 function

www.nature.com/articles/s41...
ATP plays a structural role in Hsp90 function - Nature Communications
The exact role of ATP in the function of Hsp90, an essential molecular chaperone, is unclear since ATP binding but not hydrolysis is essential for viability. Here, the authors propose that ATP plays a...
www.nature.com
August 12, 2025 at 9:02 PM
Reposted by Lakshita Sharma
walterwchen.bsky.social
Our work on new paradigm in peroxisome biogenesis and PEX39 (1st human peroxisomal biogenesis protein (peroxin/PEX) found in > 20 yr) is out in @natcellbio.nature.com! Equal collab w/Tony Rodrigues (Jorge Azevedo lab), @dwendscheck.bsky.social (Bettina Warscheid lab)

www.nature.com/articles/s41...
PEX39 facilitates the peroxisomal import of PTS2-containing proteins - Nature Cell Biology
Chen et al. show that PEX39 cooperates with PEX7 in the peroxisomal import of proteins containing a PTS2 site and uncover an (R/K)PWE motif in PEX39 and PEX13 that binds to PEX7 and facilitates the im...
www.nature.com
July 30, 2025 at 12:16 PM
Reposted by Lakshita Sharma
apatgiri.bsky.social
Silencing mitochondrial gene expression in living cells www.science.org/doi/10.1126/...
Silencing mitochondrial gene expression in living cells
Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms u...
www.science.org
May 24, 2025 at 10:24 AM
Reposted by Lakshita Sharma
jcellsci.bsky.social
Kailash Venkatraman, Nicolas-Frédéric Lipp & @ibudin.bsky.social examine the similarities between prokaryotic intracytoplasmic membranes & mitochondrial IMs, & discuss whether cristae evolution has driven specialisation of the #mito lipidome.
journals.biologists.com/jcs/article/...
#JCSMitoSI
Bacterial ICMs exhibit internal invaginations that resemble eukaryotic cristae. (A)TEM images showing tubular cristae-like ICM compartments in free-living Desulfobacterota, and similar cristae-like ICM tubules in Desulfovibrio carbinolicus strains. The eukaryotic model organism S. cerevisiae exhibits tubular cristae structures, whereas the commonly used HEK293 cell line exhibits predominantly lamellar cristae structures. Scale bars: 500 nm. (B) Eukaryotic mitochondria tend to exhibit lamellar, tubular or discoidal cristae morphology, whereas orders of the class alphaproteobacteria exhibit a range of ICM morphologies. Lamellar-like ICMs, which exhibit parallel cytoplasmic protrusions that do not contact the inner membrane, are commonly observed in nitrogen-fixing and several methanotrophic alphaproteobacteria. (C) This simplified and non-exhaustive cladogram indicates the two domains of life – bacteria and archaea – with proto-mitochondria emerging in early alphaproteobacteria. Eukaryogenesis is marked by the horizontal transfer (red dotted arrow) from Bacteria to Hordarchaeales in Asgard archaea. §, †, #, * and ‡ connect species listed in B to their respective clades in C.
May 12, 2025 at 9:05 AM
Reposted by Lakshita Sharma
pwydorski.bsky.social
"Scouring the human Hsp70 network uncovers diverse chaperone safeguards buffering TDP-43 toxicity"
www.biorxiv.org/content/10.1...
Scouring the human Hsp70 network uncovers diverse chaperone safeguards buffering TDP-43 toxicity
Cytoplasmic aggregation and concomitant dysfunction of the prion-like, RNA-binding protein TDP-43 underpin several fatal neurodegenerative diseases, including amyotrophic lateral sclerosis. To elucida...
www.biorxiv.org
May 11, 2025 at 1:47 PM
Reposted by Lakshita Sharma
hirakdas.bsky.social
Happy to share our recent publication where we present the most up to date membrane protein inventory of #Glycosomes and identified the long sought trypanosomal PEX15.

Thanks to everyone involved for this amazing collaborative effort to combine #proteomics with #functional #analysis.
April 28, 2025 at 2:21 PM
Reposted by Lakshita Sharma
frezzalab.bsky.social
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis: Molecular Cell www.cell.com/molecular-ce...
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis
Mitochondria are metabolic hubs that communicate with other organelles via metabolite-, lipid-, and signaling-molecule exchange facilitated by membrane contact sites (MCSs). MCSs, regulated by tetheri...
www.cell.com
April 18, 2025 at 6:53 AM
Reposted by Lakshita Sharma
dplamartin.bsky.social
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵

www.science.org/doi/10.1126/...
Retromer promotes the lysosomal turnover of mtDNA
Lysosomal uptake of mtDNA controls mitochondrial quality.
www.science.org
April 4, 2025 at 6:42 PM
Reposted by Lakshita Sharma
slavov-n.bsky.social
Timescales in Cell Biology
April 4, 2025 at 11:32 AM
Reposted by Lakshita Sharma
korolchuklab.bsky.social
www.cell.com/trends/cell-...
The fate of mitochondrial respiratory complexes in aging
While mitochondrial dysfunction is one of the canonical hallmarks of aging, it remains only vaguely defined. Its core feature embraces defects in energy-producing molecular machinery, the mitochondria...
www.cell.com
March 27, 2025 at 9:32 AM
lakshitaaa.bsky.social
"When life gives you a 3D printer, you print a duck… that actually floats! Lab innovation at its finest. #ScienceMeetsQuack

@warscheidlab.bsky.social
March 26, 2025 at 8:20 AM
Reposted by Lakshita Sharma
odedrechavi.bsky.social
My face on zoom when i pretend to listen but work on something else
March 11, 2025 at 7:55 AM
Reposted by Lakshita Sharma
natcellbio.nature.com
☕️Interested in how cells respond to #mitochondria #ProteinImport stress? Read our Review article by Nikolaus Pfanner, Fabian den Brave & Thomas Becker.
👉🏽https://rdcu.be/ecb3x
bit.ly/3DgVkm5
Mitochondrial protein import stress - Nature Cell Biology
Cells respond to mitochondrial protein import stress by regenerating clogged import sites and inducing stress responses. Mitochondria are thus tightly integrated into the cellular proteostasis and str...
bit.ly
March 4, 2025 at 10:27 PM
Reposted by Lakshita Sharma
clausenlab.bsky.social
DUBs - unsung yet exciting players in the UPS: kay hofmann et al reveal bacterial DUBs with irreversible clippase activity, reshaping our view on ubiquitin dynamics ... and providing a tool to distinguish Ub and ISG15 signals in immune signalling. chapeau! www.sciencedirect.com/science/arti...
A family of bacterial Josephin-like deubiquitinases with an irreversible cleavage mode
Many intracellular bacteria secrete deubiquitinase (DUB) effectors into eukaryotic host cells to keep the bacterial surface or the enclosing vesicle m…
www.sciencedirect.com
March 4, 2025 at 8:40 AM
Reposted by Lakshita Sharma
labfriedman.bsky.social
Some good news (and fresh science) to break things up! Our work identifying a subpopulation of compositionally unique mitochondria that localize to filopodia and promote cell migration is now @currentbiology.bsky.social! Led by fantastic @madeleinempavey.bsky.social!

www.cell.com/current-biol...
Compositionally unique mitochondria in filopodia support cellular migration
Marlar-Pavey et al. determine that a subpopulation of compositionally unique mitochondria, METEORs, dynamically localize to filopodia. Loss of mitochondrial targeting to filopodia correlates with a re...
www.cell.com
February 19, 2025 at 4:06 PM
lakshitaaa.bsky.social
Had an absolute blast this week! ✨🚀 The excitement is off the charts as we gear up to dive into new frontiers with the Astral mass spectrometer. Ready to unlock new dimensions of identification and discovery—stay tuned for the magic that’s about to unfold! 🤓🔬🔍 #MassSpecMagic #AstralVibes"
dwendscheck.bsky.social Daniel Wendscheck @dwendscheck.bsky.social · Feb 14
Happy @warscheidlab.bsky.social Team after three days training on the Thermo #Astral. Many thanks to Michal Nadler Holly for such a great course! #TeamMassSpec #proteomics #PhDlife @lakshitaaa.bsky.social @johannes-uni-wue.bsky.social @hirakdas.bsky.social
February 14, 2025 at 3:45 PM
lakshitaaa.bsky.social
🤯🤯
pastelbio.bsky.social PastelBio @pastelbio.bsky.social · Jan 16
Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity www.nature.com/artic...

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#proteomics #prot-paper
January 16, 2025 at 4:48 PM
Reposted by Lakshita Sharma
cellsandprots.bsky.social
Protein quality control is a tug-of-war between degradation machinery and chaperones that promote folding. Thrilled to see our latest manuscript is now published!
👇 Follow 🧵 for quick summary
journals.biologists.com/jcs/article-...
January 10, 2025 at 5:10 PM
Reposted by Lakshita Sharma
kusterlab.bsky.social
🎉 We're happy to announce that our latest project was published in @naturecomms.bsky.social this week: PTMNavigator, a #bioinformatics web platform for in-depth analysis of post-translational modification (PTM) perturbation datasets.
📄 doi.org/10.1038/s414... (1/6)
PTMNavigator: interactive visualization of differentially regulated post-translational modifications in cellular signaling pathways - Nature Communications
Post-translational modifications are important regulators of cellular pathways, but our understanding of these processes is limited. Here, the authors present a web tool that integrates various databa...
doi.org
January 10, 2025 at 2:20 PM
Reposted by Lakshita Sharma
mitocrue.bsky.social
www.nature.com/articles/s41...
Mitochondrial YME1L1 governs unoccupied protein translocase channels - Nature Cell Biology
Hsu et al. show that mitochondrial import blockage stress activates the ATP-dependent protease YME1L1, which degrades mitochondrial presequence translocase TIM23 subunits to promote cell growth.
www.nature.com
January 7, 2025 at 10:05 PM
Reposted by Lakshita Sharma
dwendscheck.bsky.social
Post-Doc Position WANTED⚡️
I'll finish my PhD work within the next few month and started to sort out where to continue doing exciting science. Any recommendations & suggestions are welcome🙏 More information on my scientific background and ideas for the future below 🧵 reskeet very much appreciated!
December 22, 2024 at 6:21 AM
Reposted by Lakshita Sharma
johannes-uni-wue.bsky.social
Who is messing with your favorite protein Filamin C in stressed muscle cells? 🧩🔬 We tackled this riddle in our latest paper in Scientific Reports. A huge shoutout to former lab mate Anja and Thomas from the @mkoehn.bsky.social lab - this was a true team effort! 👏🎉! A short thread: 🧵(1/5)
December 20, 2024 at 2:58 PM