Evan Healy
@evhealy.bsky.social
Research fellow studying all things chromatin, transcription & RNA
Monash University, Melbourne, Australia
🇮🇪
Monash University, Melbourne, Australia
🇮🇪
Reposted by Evan Healy
Very happy to see our paper published online natcomms.nature.com. Thank you to @wellcometrust.bsky.social for funding this work during my time with @robklose.bsky.social and David Booth! Thanks also to collaborators @garcialabms.bsky.social @alexdemendoza.bsky.social and the other authors!
Chromatin profiling identifies putative dual roles for H3K27me3 in regulating cell type-specific genes and transposable elements in choanoflagellates
Nature Communications - Here, the authors investigate chromatin-based gene regulation in the closest relative of animal, choanoflagellates. They uncover a putative dual role for the histone...
rdcu.be
October 29, 2025 at 1:40 PM
Very happy to see our paper published online natcomms.nature.com. Thank you to @wellcometrust.bsky.social for funding this work during my time with @robklose.bsky.social and David Booth! Thanks also to collaborators @garcialabms.bsky.social @alexdemendoza.bsky.social and the other authors!
Reposted by Evan Healy
1/ 🚀 AEBP2 isn’t what we thought.
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
October 31, 2025 at 10:53 AM
1/ 🚀 AEBP2 isn’t what we thought.
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
Reposted by Evan Healy
Come join us in Geneva for everything epigenetics and gene regulation. It will be a great meeting! Please repost!
www.keystonesymposia.org/conferences/...
www.keystonesymposia.org/conferences/...
Epigenetics and Gene Regulation in Health and Disease: Linking Basic Mechanisms with Therapeutic Opportunities | Keystone Symposia
Join us at the Keystone Symposia on Epigenetics and Gene Regulation in Health and Disease: Linking Basic Mechanisms with Therapeutic Opportunities, March 2026, in Geneva, with field leaders!
www.keystonesymposia.org
October 17, 2025 at 2:36 PM
Come join us in Geneva for everything epigenetics and gene regulation. It will be a great meeting! Please repost!
www.keystonesymposia.org/conferences/...
www.keystonesymposia.org/conferences/...
Reposted by Evan Healy
🧵1/Exciting news in cancer epigenetics! Our latest research, "AEBP2-Directed H3K27me2 Defines a Specific Vulnerability in EZH2-mutant Lymphoma", is now available on www.biorxiv.org/content/10.1.... Here's a thread summarizing our findings!👇
#CancerResearch #Epigenetics #Chromatin #Lymphoma
#CancerResearch #Epigenetics #Chromatin #Lymphoma
AEBP2-Directed H3K27me2 Defines a Specific Vulnerability in EZH2-mutant Lymphoma
The catalytic subunit of Polycomb Repressive Complex 2 (PRC2), EZH2, is recurrently mutated in 25% of diffuse large B-cell lymphomas (DLBCL), causing increased H3K27me3 and decreased H3K27me2 levels. ...
www.biorxiv.org
October 17, 2025 at 7:11 AM
🧵1/Exciting news in cancer epigenetics! Our latest research, "AEBP2-Directed H3K27me2 Defines a Specific Vulnerability in EZH2-mutant Lymphoma", is now available on www.biorxiv.org/content/10.1.... Here's a thread summarizing our findings!👇
#CancerResearch #Epigenetics #Chromatin #Lymphoma
#CancerResearch #Epigenetics #Chromatin #Lymphoma
Reposted by Evan Healy
Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @cp-cell.bsky.social, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. www.sciencedirect.com/science/arti...
July 26, 2025 at 7:15 PM
Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @cp-cell.bsky.social, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. www.sciencedirect.com/science/arti...
Reposted by Evan Healy
We are looking for new colleagues to come join us in Galway as group leaders (Junior and Senior). The Centre for Chromosome Biology is a great place and it is a good time to join. Please reach out if you want to chat about the opportunity!
www.nature.com/naturecareer...
www.nature.com/naturecareer...
July 16, 2025 at 8:56 AM
We are looking for new colleagues to come join us in Galway as group leaders (Junior and Senior). The Centre for Chromosome Biology is a great place and it is a good time to join. Please reach out if you want to chat about the opportunity!
www.nature.com/naturecareer...
www.nature.com/naturecareer...
Reposted by Evan Healy
I am looking for a new postdoc to join the lab. Interested in pluripotency, germ cells, and in investigating these in a genetically tractable cnidarian? Get in touch! We offer a long-term contract, excellent research environment, and a lovely city #Galway. www.urifranklab.org
June 20, 2025 at 1:24 PM
I am looking for a new postdoc to join the lab. Interested in pluripotency, germ cells, and in investigating these in a genetically tractable cnidarian? Get in touch! We offer a long-term contract, excellent research environment, and a lovely city #Galway. www.urifranklab.org
Reposted by Evan Healy
My group is hiring a new Postdoc to work on gene regulation and chromatin in Nematostella. Please RT!
For more information see here:
gahanlab.com
tinyurl.com/34xke35d
For more information see here:
gahanlab.com
tinyurl.com/34xke35d
April 8, 2025 at 12:09 PM
My group is hiring a new Postdoc to work on gene regulation and chromatin in Nematostella. Please RT!
For more information see here:
gahanlab.com
tinyurl.com/34xke35d
For more information see here:
gahanlab.com
tinyurl.com/34xke35d
Reposted by Evan Healy
Check out this 🧵 from @eimearlagan.bsky.social on our new Molecular Cell @cp-molcell.bsky.social paper! We show how the H3K27M Oncohistone reprograms chromatin in DMG, creating a specific dependency on CBX4/PCGF4-containing forms of cPRC1 👇
Excited to share our new paper out today in @cp-molcell.bsky.social! We show that the H3K27M oncohistone rewires cPRC1, creating a unique dependency on CBX4/PCGF4-containing complexes, and also reveal a previously unknown function of CBX4. Highlights below (1/11).
May 21, 2025 at 5:22 PM
Check out this 🧵 from @eimearlagan.bsky.social on our new Molecular Cell @cp-molcell.bsky.social paper! We show how the H3K27M Oncohistone reprograms chromatin in DMG, creating a specific dependency on CBX4/PCGF4-containing forms of cPRC1 👇
Reposted by Evan Healy
Excited to share our new paper out today in @cp-molcell.bsky.social! We show that the H3K27M oncohistone rewires cPRC1, creating a unique dependency on CBX4/PCGF4-containing complexes, and also reveal a previously unknown function of CBX4. Highlights below (1/11).
May 21, 2025 at 4:13 PM
Excited to share our new paper out today in @cp-molcell.bsky.social! We show that the H3K27M oncohistone rewires cPRC1, creating a unique dependency on CBX4/PCGF4-containing complexes, and also reveal a previously unknown function of CBX4. Highlights below (1/11).
Reposted by Evan Healy
Delighted to share the latest preprint from the lab led by Janith Seneviratne
We reveal novel roles for #embryonic regulators DPPA2/4 in facilitating a H2AK119Ub primed #chromatin state when aberrantly up-regulated in non-small cell lung #cancers 🧬🔬 (1/6)
www.biorxiv.org/content/10.1...
We reveal novel roles for #embryonic regulators DPPA2/4 in facilitating a H2AK119Ub primed #chromatin state when aberrantly up-regulated in non-small cell lung #cancers 🧬🔬 (1/6)
www.biorxiv.org/content/10.1...
April 28, 2025 at 11:07 AM
Delighted to share the latest preprint from the lab led by Janith Seneviratne
We reveal novel roles for #embryonic regulators DPPA2/4 in facilitating a H2AK119Ub primed #chromatin state when aberrantly up-regulated in non-small cell lung #cancers 🧬🔬 (1/6)
www.biorxiv.org/content/10.1...
We reveal novel roles for #embryonic regulators DPPA2/4 in facilitating a H2AK119Ub primed #chromatin state when aberrantly up-regulated in non-small cell lung #cancers 🧬🔬 (1/6)
www.biorxiv.org/content/10.1...
Reposted by Evan Healy
👋 Meet our new Scientific Head - Professor Jose Polo!
A world leader in epigenetics & stem cell biology, Prof Polo (SAiGENCI, University of Adelaide, Monash University) brings his deep expertise & passion for advancing life sciences in Aus & beyond. 🌏
We're thrilled to have him on board! 👏
A world leader in epigenetics & stem cell biology, Prof Polo (SAiGENCI, University of Adelaide, Monash University) brings his deep expertise & passion for advancing life sciences in Aus & beyond. 🌏
We're thrilled to have him on board! 👏
Prof Jose Polo appointed as EMBL Australia Scientific Head | EMBL Australia
Professor Jose Polo has been appointed as the new Scientific Head of EMBL Australia. A globally recognised leader in epigenetics and stem cell biology, Prof Polo brings extensive expertise and a passi...
www.emblaustralia.org
April 16, 2025 at 11:42 PM
👋 Meet our new Scientific Head - Professor Jose Polo!
A world leader in epigenetics & stem cell biology, Prof Polo (SAiGENCI, University of Adelaide, Monash University) brings his deep expertise & passion for advancing life sciences in Aus & beyond. 🌏
We're thrilled to have him on board! 👏
A world leader in epigenetics & stem cell biology, Prof Polo (SAiGENCI, University of Adelaide, Monash University) brings his deep expertise & passion for advancing life sciences in Aus & beyond. 🌏
We're thrilled to have him on board! 👏
Reposted by Evan Healy
📣 A reminder about an open postdoctoral position in our group!
🧫 Are you interested in mechanisms of gene regulation, human facial development and disease? How our DNA shapes the way we look? Do you love chromatin and enhancers? 🧬
If so, reach out for an informal chat! 💬
longlab.co.uk
🧫 Are you interested in mechanisms of gene regulation, human facial development and disease? How our DNA shapes the way we look? Do you love chromatin and enhancers? 🧬
If so, reach out for an informal chat! 💬
longlab.co.uk
Postdoctoral Researcher
Our research is focused on understanding how genetic changes in the non-coding genome can impact gene regulatory mechanisms, alter developmental processes and ultimately affect human craniofacial shap...
elxw.fa.em3.oraclecloud.com
April 15, 2025 at 9:17 AM
📣 A reminder about an open postdoctoral position in our group!
🧫 Are you interested in mechanisms of gene regulation, human facial development and disease? How our DNA shapes the way we look? Do you love chromatin and enhancers? 🧬
If so, reach out for an informal chat! 💬
longlab.co.uk
🧫 Are you interested in mechanisms of gene regulation, human facial development and disease? How our DNA shapes the way we look? Do you love chromatin and enhancers? 🧬
If so, reach out for an informal chat! 💬
longlab.co.uk
Reposted by Evan Healy
The Americans are doing to Kseniia what the Russians did to Brittney Griner for having 0.7 grams of cannabis oil. Except no one is coming to negotiate her freedom and Harvard is sitting on their hands. I am so ashamed. Please share this story.
www.nytimes.com/2025/04/11/s...
www.nytimes.com/2025/04/11/s...
She Worked in a Harvard Lab to Reverse Aging, Until ICE Jailed Her (Gift Article)
President Trump’s immigration crackdown ensnared Kseniia Petrova, a scientist who fled Russia after protesting its invasion of Ukraine. She fears arrest if she is deported there.
www.nytimes.com
April 11, 2025 at 5:55 PM
The Americans are doing to Kseniia what the Russians did to Brittney Griner for having 0.7 grams of cannabis oil. Except no one is coming to negotiate her freedom and Harvard is sitting on their hands. I am so ashamed. Please share this story.
www.nytimes.com/2025/04/11/s...
www.nytimes.com/2025/04/11/s...
Reposted by Evan Healy
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Evan Healy
1/ H3K27me3 mimicry has repeatedly emerged through evolution, but what's the physiological relevance?
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
March 15, 2025 at 12:12 AM
1/ H3K27me3 mimicry has repeatedly emerged through evolution, but what's the physiological relevance?
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
Reposted by Evan Healy
Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains https://www.biorxiv.org/content/10.1101/2025.03.13.642955v1
March 14, 2025 at 3:19 AM
Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains https://www.biorxiv.org/content/10.1101/2025.03.13.642955v1
Reposted by Evan Healy
We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...
Dynamic PRC1–CBX8 stabilizes a porous structure of chromatin condensates - Nature Structural & Molecular Biology
Here the authors show that a gene-inactivating protein complex packs inactive genes into a dynamic and accessible structure. The study challenges the traditional views that restricted accessibility an...
www.nature.com
January 16, 2025 at 9:24 PM
We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...
Reposted by Evan Healy
Please see 🧵by @evhealy.bsky.social about our new paper exploring the role of PRC1 and PRC2 in non-dividing cells 👇
🧵 1/ We’re excited to share that our new paper with @adrianbracken.bsky.social lab is out 🎉 In this study (which began over 10 years ago!), we explore the biology of PRC2 and PRC1 in non-dividing cells. We also explore the effects of PRC2 inhibitor drugs on these cells. Here’s what we found👇
January 14, 2025 at 11:03 PM
Please see 🧵by @evhealy.bsky.social about our new paper exploring the role of PRC1 and PRC2 in non-dividing cells 👇
🧵 1/ We’re excited to share that our new paper with @adrianbracken.bsky.social lab is out 🎉 In this study (which began over 10 years ago!), we explore the biology of PRC2 and PRC1 in non-dividing cells. We also explore the effects of PRC2 inhibitor drugs on these cells. Here’s what we found👇
January 14, 2025 at 10:57 PM
🧵 1/ We’re excited to share that our new paper with @adrianbracken.bsky.social lab is out 🎉 In this study (which began over 10 years ago!), we explore the biology of PRC2 and PRC1 in non-dividing cells. We also explore the effects of PRC2 inhibitor drugs on these cells. Here’s what we found👇
Reposted by Evan Healy
Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis for the inhibition of PRC2 by active transcription histone posttranslational modifications - Nature Structural & Molecular Biology
Structures reveal that histone H3K36me3 and H3K4me3 modifications reduce Polycomb repressive complex 2 (PRC2) function through the inhibition of histone tail engagement and antagonistic binding to the...
www.nature.com
January 8, 2025 at 1:36 AM
Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Evan Healy
"Everything in epigenetics is circular". Here, we explore how memory that is stored locally on chromatin has a crucial role in stabilising cellular state, updating our view of the epigenetic landscape from one shaped by the genotype, to one moulded by experience
doi.org/10.1186/s130...
doi.org/10.1186/s130...
Chromatin-based memory as a self-stabilizing influence on cell identity - Genome Biology
Cell types are traditionally thought to be specified and stabilized by gene regulatory networks. Here, we explore how chromatin memory contributes to the specification and stabilization of cell states...
doi.org
December 30, 2024 at 10:58 PM
"Everything in epigenetics is circular". Here, we explore how memory that is stored locally on chromatin has a crucial role in stabilising cellular state, updating our view of the epigenetic landscape from one shaped by the genotype, to one moulded by experience
doi.org/10.1186/s130...
doi.org/10.1186/s130...
Reposted by Evan Healy
AEpiA 2024 ORAL PRESENTATION PRIZE WINNERS
1st - Mark Ezegboyu (University of Otago)
2nd - @evhealy.bsky.social (Monash University)
Runners up - Braydon Meyer (@garvaninstitute.bsky.social), Kate O'Hara (@csiro.bsky.social) and @scientistkate.bsky.social (@cmri.bsky.social)
1st - Mark Ezegboyu (University of Otago)
2nd - @evhealy.bsky.social (Monash University)
Runners up - Braydon Meyer (@garvaninstitute.bsky.social), Kate O'Hara (@csiro.bsky.social) and @scientistkate.bsky.social (@cmri.bsky.social)
December 17, 2024 at 4:11 AM
AEpiA 2024 ORAL PRESENTATION PRIZE WINNERS
1st - Mark Ezegboyu (University of Otago)
2nd - @evhealy.bsky.social (Monash University)
Runners up - Braydon Meyer (@garvaninstitute.bsky.social), Kate O'Hara (@csiro.bsky.social) and @scientistkate.bsky.social (@cmri.bsky.social)
1st - Mark Ezegboyu (University of Otago)
2nd - @evhealy.bsky.social (Monash University)
Runners up - Braydon Meyer (@garvaninstitute.bsky.social), Kate O'Hara (@csiro.bsky.social) and @scientistkate.bsky.social (@cmri.bsky.social)
Reposted by Evan Healy
A preview on the latest work from @robklose.bsky.social lab where Aleks (Not made the bluesky transition yet) used single molecule approaches to address how Polycomb controls transcription. He found that Polycomb limits the frequency with which a promoter can enter a permissive transcribing state.
November 21, 2024 at 8:00 AM
A preview on the latest work from @robklose.bsky.social lab where Aleks (Not made the bluesky transition yet) used single molecule approaches to address how Polycomb controls transcription. He found that Polycomb limits the frequency with which a promoter can enter a permissive transcribing state.
Reposted by Evan Healy
A report made counterintuitive claims that PRC2 binds
→ more RNA than PTBP1 & hnRNPU
→ RNAs that do not exist in the cell
We found a simple explanation: the authors ignore most RNA in the sample, leading to inflated background & misleading conclusions.
www.biorxiv.org/content/10.1...
→ more RNA than PTBP1 & hnRNPU
→ RNAs that do not exist in the cell
We found a simple explanation: the authors ignore most RNA in the sample, leading to inflated background & misleading conclusions.
www.biorxiv.org/content/10.1...
Failing to account for RNA quantity inflates background and leads to the misleading appearance that PRC2 and GFP bind to RNA in vivo
We recently published biochemical and quantitative evidence that challenges the widespread claims that PRC2 binds directly to many RNAs in vivo . A recent preprint performs a re-analysis of some of ou...
www.biorxiv.org
November 18, 2024 at 1:11 AM
A report made counterintuitive claims that PRC2 binds
→ more RNA than PTBP1 & hnRNPU
→ RNAs that do not exist in the cell
We found a simple explanation: the authors ignore most RNA in the sample, leading to inflated background & misleading conclusions.
www.biorxiv.org/content/10.1...
→ more RNA than PTBP1 & hnRNPU
→ RNAs that do not exist in the cell
We found a simple explanation: the authors ignore most RNA in the sample, leading to inflated background & misleading conclusions.
www.biorxiv.org/content/10.1...