Reposted by Daniel McManus, PhD
In our latest study in @cp-immunity.bsky.social, we followed dynamics of vaccine-specific Tfh cells for 60+ weeks after immunization.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
November 12, 2025 at 7:45 PM
In our latest study in @cp-immunity.bsky.social, we followed dynamics of vaccine-specific Tfh cells for 60+ weeks after immunization.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
Reposted by Daniel McManus, PhD
Reposted by Daniel McManus, PhD
Congratulations to Fred Ramsdell, Mary Brunkow and Shimon Sakaguchi - the 2025 Winners of the Nobel Prize in Physiology or Medicine. #Treg_cells #Foxp3 #Immune_regulation
October 6, 2025 at 3:37 PM
Congratulations to Fred Ramsdell, Mary Brunkow and Shimon Sakaguchi - the 2025 Winners of the Nobel Prize in Physiology or Medicine. #Treg_cells #Foxp3 #Immune_regulation
Reposted by Daniel McManus, PhD
CD7 regulates the persistence of terminally exhausted CD8+ T cells during chronic infection @cp-cellreports.bsky.social
www.cell.com/cell-reports... @bcmhouston.bsky.social
www.cell.com/cell-reports... @bcmhouston.bsky.social
September 22, 2025 at 4:32 PM
CD7 regulates the persistence of terminally exhausted CD8+ T cells during chronic infection @cp-cellreports.bsky.social
www.cell.com/cell-reports... @bcmhouston.bsky.social
www.cell.com/cell-reports... @bcmhouston.bsky.social
Great new work from Will Hudson’s lab at @bcmhouston.bsky.social on the role of CD7 during CD8 T cell exhaustion! tinyurl.com/3c5mdyzr
hudsonlaboratory.com
@cp-cellreports.bsky.social
hudsonlaboratory.com
@cp-cellreports.bsky.social
CD7 regulates the persistence of terminally exhausted CD8+ T cells during chronic infection
Hyslop et al. identify CD7 as a tissue-specific receptor essential for maintaining
terminally exhausted CD8+ T cells during chronic infection. CD7 is highly expressed
on a CD101+Tim3low liver-resident...
tinyurl.com
September 22, 2025 at 7:19 PM
Great new work from Will Hudson’s lab at @bcmhouston.bsky.social on the role of CD7 during CD8 T cell exhaustion! tinyurl.com/3c5mdyzr
hudsonlaboratory.com
@cp-cellreports.bsky.social
hudsonlaboratory.com
@cp-cellreports.bsky.social
Reposted by Daniel McManus, PhD
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com
September 19, 2025 at 2:39 PM
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Daniel McManus, PhD
We are looking to hire post-docs to work on T cell responses to M. tuberculosis infection using murine and NHP models. If you know T cells and wanna work on the top global infectious killer check us out. BSL3 experience not required. The NIH intramural program in Bethesda MD is an amazing place!
September 17, 2025 at 5:50 PM
We are looking to hire post-docs to work on T cell responses to M. tuberculosis infection using murine and NHP models. If you know T cells and wanna work on the top global infectious killer check us out. BSL3 experience not required. The NIH intramural program in Bethesda MD is an amazing place!
Reposted by Daniel McManus, PhD
I’m pleased to share my latest work, published in Science Advances.
In this study, we investigated how fibroblastic reticular cells (FRCs) that populate the white pulp of the spleen regulate immune responses to viral infections.
#immunology #immunosky
www.science.org/doi/10.1126/...
In this study, we investigated how fibroblastic reticular cells (FRCs) that populate the white pulp of the spleen regulate immune responses to viral infections.
#immunology #immunosky
www.science.org/doi/10.1126/...
Splenic fibroblastic reticular cells orchestrate dendritic cell maturation and facilitate CD8+ T cell priming and protective memory
Fibroblastic reticular cells of the spleen coordinate inflammation for optimal antiviral T cell responses and protective memory.
www.science.org
June 5, 2025 at 12:32 AM
I’m pleased to share my latest work, published in Science Advances.
In this study, we investigated how fibroblastic reticular cells (FRCs) that populate the white pulp of the spleen regulate immune responses to viral infections.
#immunology #immunosky
www.science.org/doi/10.1126/...
In this study, we investigated how fibroblastic reticular cells (FRCs) that populate the white pulp of the spleen regulate immune responses to viral infections.
#immunology #immunosky
www.science.org/doi/10.1126/...
Reposted by Daniel McManus, PhD
One of our obsessions in the Crotty lab is to better understand B-cell immunology in the context of vaccines. Rare B cells can have antibody specificities particularly important for protection against some diseases. In our most recent paper…
@cp-immunity.bsky.social
www.cell.com/immunity/ful...
@cp-immunity.bsky.social
www.cell.com/immunity/ful...
Diverse priming outcomes under conditions of very rare precursor B cells
B cells that give rise to neutralizing antibody responses can be extremely rare in
the naive B cell repertoire. Madden et al. show that delivery strategy and adjuvant
selection affect the recruitment ...
www.cell.com
April 7, 2025 at 1:50 PM
One of our obsessions in the Crotty lab is to better understand B-cell immunology in the context of vaccines. Rare B cells can have antibody specificities particularly important for protection against some diseases. In our most recent paper…
@cp-immunity.bsky.social
www.cell.com/immunity/ful...
@cp-immunity.bsky.social
www.cell.com/immunity/ful...
Reposted by Daniel McManus, PhD
Fantastic talk and beautiful story from Maria Cardenas and @haydnkissick.bsky.social lab!
Hosted by @openboxscience.bsky.social
youtu.be/rC69rAz6OeE
Hosted by @openboxscience.bsky.social
youtu.be/rC69rAz6OeE
[Maria Cardenas] Differentiation fate of a stem-like CD4 T cell controls immunity to cancer
YouTube video by Open Box Science
youtu.be
March 14, 2025 at 5:21 PM
Fantastic talk and beautiful story from Maria Cardenas and @haydnkissick.bsky.social lab!
Hosted by @openboxscience.bsky.social
youtu.be/rC69rAz6OeE
Hosted by @openboxscience.bsky.social
youtu.be/rC69rAz6OeE
Reposted by Daniel McManus, PhD
Very excited our work out in @jem.org “Transient inhibition of type I interferon enhances CD8+ T cell stemness and vaccine protection”
@wehi-research.bsky.social led by talented (and determined) PhD student @benjbroom.bsky.social
Thread below.
@wehi-research.bsky.social led by talented (and determined) PhD student @benjbroom.bsky.social
Thread below.
@benjbroom.bsky.social et al. @groomlab.bsky.social show that early type I interferon blockade alters CXCR3 chemokine regulation & cell location to promote an antigen-dependent CD8+ T cell transition of precursor of exhausted T cells to stem cell–like memory CD8+ T cells rupress.org/jem/article/...
March 11, 2025 at 3:26 AM
Very excited our work out in @jem.org “Transient inhibition of type I interferon enhances CD8+ T cell stemness and vaccine protection”
@wehi-research.bsky.social led by talented (and determined) PhD student @benjbroom.bsky.social
Thread below.
@wehi-research.bsky.social led by talented (and determined) PhD student @benjbroom.bsky.social
Thread below.
Reposted by Daniel McManus, PhD
Excellent Info Graphic. Share everywhere!
February 23, 2025 at 4:33 PM
Excellent Info Graphic. Share everywhere!
Reposted by Daniel McManus, PhD
Using PAXgene as an alternative tissue preservative for spatial transcriptomics with #Xenium @10xgenomics.bsky.social - An insane amount of work and a very methodical investigation
www.biorxiv.org
February 19, 2025 at 2:57 AM
Using PAXgene as an alternative tissue preservative for spatial transcriptomics with #Xenium @10xgenomics.bsky.social - An insane amount of work and a very methodical investigation
Reposted by Daniel McManus, PhD
My wife and I were postbac IRTAs 25 years ago. Neither would have pursued a career in research if not for this program
We have PhD students in Yale Immunobiology now who also came from this NIH program including (one amazing student in my lab)
This is a valuable program and has been for decades.
We have PhD students in Yale Immunobiology now who also came from this NIH program including (one amazing student in my lab)
This is a valuable program and has been for decades.
One of the many, many “pauses” and cancellations devastating our next generation of researchers. “The NIH Intramural Program has paused the recruitment of IRTAs, CRTAs, and Visiting Fellows in all training programs pending guidance from Health and Human Services. Check back daily for updates.”
Postbac Program
www.training.nih.gov
February 18, 2025 at 10:58 AM
My wife and I were postbac IRTAs 25 years ago. Neither would have pursued a career in research if not for this program
We have PhD students in Yale Immunobiology now who also came from this NIH program including (one amazing student in my lab)
This is a valuable program and has been for decades.
We have PhD students in Yale Immunobiology now who also came from this NIH program including (one amazing student in my lab)
This is a valuable program and has been for decades.
Reposted by Daniel McManus, PhD
I’m excited to share our lab’s new preprint! We find that some exhausted T cell clones maintain the capacity form functional memory cells:
www.biorxiv.org/content/10.1...
1/ 🧵
www.biorxiv.org/content/10.1...
1/ 🧵
www.biorxiv.org
February 16, 2025 at 3:46 PM
I’m excited to share our lab’s new preprint! We find that some exhausted T cell clones maintain the capacity form functional memory cells:
www.biorxiv.org/content/10.1...
1/ 🧵
www.biorxiv.org/content/10.1...
1/ 🧵
Reposted by Daniel McManus, PhD
Excited for our lab's FIRST paper #ScienceImmunology! 🎉
Led by @categraca.bsky.social & Amania Sheikh, with amazing collabs from Axel Kallies, Dane Newman, & @rickyjohnstone.bsky.social
Huge thanks to reviewers & @claireolingy.bsky.social 🙌
#Tpex #onthescoreboard
www.science.org/doi/full/10....
Led by @categraca.bsky.social & Amania Sheikh, with amazing collabs from Axel Kallies, Dane Newman, & @rickyjohnstone.bsky.social
Huge thanks to reviewers & @claireolingy.bsky.social 🙌
#Tpex #onthescoreboard
www.science.org/doi/full/10....
Stem-like memory and precursors of exhausted T cells share a common progenitor defined by ID3 expression
ID3 defines stem-like T cells with high developmental potential that sustain T cell responses to chronic infection and cancer.
www.science.org
January 31, 2025 at 7:51 PM
Excited for our lab's FIRST paper #ScienceImmunology! 🎉
Led by @categraca.bsky.social & Amania Sheikh, with amazing collabs from Axel Kallies, Dane Newman, & @rickyjohnstone.bsky.social
Huge thanks to reviewers & @claireolingy.bsky.social 🙌
#Tpex #onthescoreboard
www.science.org/doi/full/10....
Led by @categraca.bsky.social & Amania Sheikh, with amazing collabs from Axel Kallies, Dane Newman, & @rickyjohnstone.bsky.social
Huge thanks to reviewers & @claireolingy.bsky.social 🙌
#Tpex #onthescoreboard
www.science.org/doi/full/10....
Reposted by Daniel McManus, PhD
January 24, 2025 at 6:51 PM
Reposted by Daniel McManus, PhD
Super excited to announce that the Joag Lab will officially open at @UW, @SCRI in March 2025! We will study the interplay between memory T cells and stromal, humoral, and innate immunity in mucosal tissues. researcherprofiles.seattlechildrens.org/Vineet.Joag. Hiring a technician and a postdoc!
January 22, 2025 at 6:14 PM
Super excited to announce that the Joag Lab will officially open at @UW, @SCRI in March 2025! We will study the interplay between memory T cells and stromal, humoral, and innate immunity in mucosal tissues. researcherprofiles.seattlechildrens.org/Vineet.Joag. Hiring a technician and a postdoc!
Excited to share my PhD work from Rafi Ahmed’s lab, now published in @nature.com. We show how CD8 T cells overcome the inability to predict how long a viral infection will last by making adaptable precursor cells a priori—an elegant strategy for a non-prescient immune system! tinyurl.com/53nrpsa6
January 15, 2025 at 10:23 PM
Excited to share my PhD work from Rafi Ahmed’s lab, now published in @nature.com. We show how CD8 T cells overcome the inability to predict how long a viral infection will last by making adaptable precursor cells a priori—an elegant strategy for a non-prescient immune system! tinyurl.com/53nrpsa6
Reposted by Daniel McManus, PhD
Two beautiful stories out this month in Nature (Jan8, 2025) about the enigmatic decision making of stem cell like CD8+ T cells from Rafi and Dietmar's labs.
Have a look!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Have a look!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
January 11, 2025 at 6:11 PM
Two beautiful stories out this month in Nature (Jan8, 2025) about the enigmatic decision making of stem cell like CD8+ T cells from Rafi and Dietmar's labs.
Have a look!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Have a look!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Daniel McManus, PhD
Two studies published in @nature.com today showing that Tpex-like cells develop independent from acute or chronic infection
#Tpex
www.nature.com/articles/s41...
#Tpex
www.nature.com/articles/s41...
January 9, 2025 at 8:33 AM
Two studies published in @nature.com today showing that Tpex-like cells develop independent from acute or chronic infection
#Tpex
www.nature.com/articles/s41...
#Tpex
www.nature.com/articles/s41...