Ruizhao WANG
@ruizhao.bsky.social
Synthetic biologist and protein engineer. Dr. (officially now) from Westlake University & Zhejiang University. Hunting for postdoc positions.
Reposted by Ruizhao WANG
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
1/n
www.biorxiv.org/content/10.1...
1/n
PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders
Proteins that bind to a target protein of interest, termed "binders," are essential components of biological research reagents and therapeutics. Target proteins present multiple binding surfaces with ...
www.biorxiv.org
November 19, 2025 at 6:10 PM
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
1/n
www.biorxiv.org/content/10.1...
1/n
Reposted by Ruizhao WANG
How do cells adapt morphology to function? In a 🔥 preprint by @zjmaggiexu.bsky.social , with @dudinlab.bsky.social and @amyweeks.bsky.social , we identify a self-organizing single-cell morphology circuit that optimizes the feeding trap structure of the suctorian P. collini. 🧵 tinyurl.com/4k8nv926
November 18, 2025 at 4:15 PM
How do cells adapt morphology to function? In a 🔥 preprint by @zjmaggiexu.bsky.social , with @dudinlab.bsky.social and @amyweeks.bsky.social , we identify a self-organizing single-cell morphology circuit that optimizes the feeding trap structure of the suctorian P. collini. 🧵 tinyurl.com/4k8nv926
Reposted by Ruizhao WANG
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - @jshendure.bsky.social @troymcdiarmid.bsky.social @uwgenome.bsky.social go.nature.com/49eTPCu
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - Nature Biotechnology
Prime editing in mammalian cells benefits from a comprehensive list of genetic parts.
go.nature.com
November 11, 2025 at 4:36 PM
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - @jshendure.bsky.social @troymcdiarmid.bsky.social @uwgenome.bsky.social go.nature.com/49eTPCu
Fun to see this thread answer some questions I had, thank you.
My initial assessment of this paper ⬇️ was brief, so let’s have a better look at it:
🧵
🧵
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
October 1, 2025 at 4:55 AM
Fun to see this thread answer some questions I had, thank you.
Cool! This could have a lot of influence on sensors, pull down, signaling rewiring...🤔
Excited to share our latest work in expanding AI-based protein design into targeting PTMs! I’m particularly excited in this work as there is so much we don’t know about PTMs despite their central role in signaling and hopefully we can start to decode their rules and functions.
Phosphorylation on tyrosines control key pathways in immunity, cancer, and metabolism. For the first time, we can now design proteins that specifically recognize individual phosphotyrosines, even in disordered regions. (1/8)
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
October 1, 2025 at 4:43 AM
Cool! This could have a lot of influence on sensors, pull down, signaling rewiring...🤔
Reposted by Ruizhao WANG
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Ruizhao WANG
PANCS-Binders (phage-assisted noncontinuous selection of protein binders) screens multiple high-diversity protein libraries against a panel of dozens of targets for high-throughput binder discovery. @chembiobryan.bsky.social @mstyles-chembiol.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 6, 2025 at 7:49 PM
PANCS-Binders (phage-assisted noncontinuous selection of protein binders) screens multiple high-diversity protein libraries against a panel of dozens of targets for high-throughput binder discovery. @chembiobryan.bsky.social @mstyles-chembiol.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ruizhao WANG
Humanized Caffeine-Inducible Systems for Controlling Cellular Functions https://www.biorxiv.org/content/10.1101/2025.06.13.659463v1
June 14, 2025 at 5:04 AM
Humanized Caffeine-Inducible Systems for Controlling Cellular Functions https://www.biorxiv.org/content/10.1101/2025.06.13.659463v1
Very cool study!
What controls condensate composition and function beyond binding stoichiometry? We show that condensate microenvironments play a key role, and can be probed by a micropeptide killswitch in live cells. www.nature.com/articles/s41... in @nature.com with @yaotianzhang.bsky.social Denes Hnisz & team.
www.nature.com
June 5, 2025 at 1:53 AM
Very cool study!
Reposted by Ruizhao WANG
Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases
In new work @arcinstitute.org, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
In new work @arcinstitute.org, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
May 15, 2025 at 1:47 PM
Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases
In new work @arcinstitute.org, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
In new work @arcinstitute.org, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
Reposted by Ruizhao WANG
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - @michellefrei17.bsky.social @jinzhanglab.bsky.social go.nature.com/3GfqrzL
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - Nature Biotechnology
Far-red kinase biosensors are applied to image kinase signaling networks at super resolution.
go.nature.com
April 21, 2025 at 2:45 PM
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - @michellefrei17.bsky.social @jinzhanglab.bsky.social go.nature.com/3GfqrzL
Reposted by Ruizhao WANG
www.cell.com/cell/fulltex...
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
Engineered nucleocytosolic vehicles for loading of programmable editors
Engineered nucleocytosolic vehicles for loading of programmable editors (ENVLPE) with
enhanced CRISPR ribonucleoprotein (RNP) loading and stabilization enable the efficient
delivery of gene-editing ef...
www.cell.com
April 9, 2025 at 11:52 PM
www.cell.com/cell/fulltex...
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
Reposted by Ruizhao WANG
LIDAR is out! rdcu.be/efugD We are one baby step closer to making it useful for therapeutics. NIH high-risk high-reward program provides key support.
March 28, 2025 at 4:44 PM
LIDAR is out! rdcu.be/efugD We are one baby step closer to making it useful for therapeutics. NIH high-risk high-reward program provides key support.
So cool!
Excited to share a new preprint! Wireless devices use FM modulation to transmit multiplexed noise-resistant data. Led by @born2raisecell.bsky.social, we create a biochemical analogue of this paradigm using genetically encoded oscillators (GEOs) for single-cell FM streaming tinyurl.com/nbs8rw42 🧵
March 5, 2025 at 11:14 AM
So cool!
Reposted by Ruizhao WANG
I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain - Nature Methods
A first-in-class fluorescence lifetime-based FRET sensor for phosphatase and tensin homolog (PTEN) enables the dynamic monitoring of PTEN activity in cultured cells and in vivo with high spatiotempora...
www.nature.com
February 20, 2025 at 1:10 PM
I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ruizhao WANG
Tetanus on demand! (not for bioweapons, but for neuroscience research)
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
January 30, 2025 at 2:30 PM
Tetanus on demand! (not for bioweapons, but for neuroscience research)
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Very cool work! Thermo-controllable protein translocation.
Excited and grateful that our initial report on temperature-sensitive protein switches is now published
@naturemethods.bsky.social Co-led by PhD students Will Benman and @dennishuang.bsky.social
www.nature.com/articles/s41...
Brief thread:
@naturemethods.bsky.social Co-led by PhD students Will Benman and @dennishuang.bsky.social
www.nature.com/articles/s41...
Brief thread:
January 26, 2025 at 6:32 AM
Very cool work! Thermo-controllable protein translocation.
Human-mid protease controls secreted proteins
Proteases might serve as versatile control knobs for gene and cell therapies, but there looms the risk of immunogenicity as we and others have been using viral proteases. We now report a control system based on a an engineered human protease and its clinically approved inhibitor. rdcu.be/d6kVt
Orthogonalized human protease control of secreted signals
Nature Chemical Biology - Engineering of a human-derived protease controlled exogenously by its FDA-approved inhibitor enables control over cytokine activity in cell-based therapies with reduced...
rdcu.be
January 18, 2025 at 7:33 AM
Human-mid protease controls secreted proteins
HaloTag goes so fascinating. Nexe step?
We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
January 8, 2025 at 8:44 AM
HaloTag goes so fascinating. Nexe step?
Reposted by Ruizhao WANG
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
While everyone esle has been concentrating on generative binder design, this group has demonstrated screening libraries in vivo! www.biorxiv.org/content/10.1...
#biosky
#biosky
High-throughput protein binder discovery by rapid in vivo selection
Proteins that selectively bind to a target of interest are foundational components of research pipelines, diagnostics, and therapeutics. Current immunization-based, display-based, and computational ap...
www.biorxiv.org
January 7, 2025 at 8:50 PM
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
I was wondering why directed evolution in mammalian cells had not yet been well developed in the last two years, and here it happens.
I'm happy to see this paper I handled finally out! -- Directed evolution in mammalian cells using an orthogonal alphaviral RNA replication system with antiviral-induced mutagenesis #ChemBio #synbio
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells - Nature Chemical Biology
An orthogonal alphaviral RNA replication system with chemically inducible control of RNA mutagenesis enables RNA-based directed evolution in mammalian cells.
www.nature.com
January 7, 2025 at 9:15 AM
I was wondering why directed evolution in mammalian cells had not yet been well developed in the last two years, and here it happens.
Reposted by Ruizhao WANG
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..
December 4, 2024 at 4:05 PM
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..