In partnership with the iPSC Neurodegenerative Disease Initiative, a project from the Center for Alzheimer’s and Related Dementias (NIH), and with support from the Chan Zuckerberg Initiative, and Alig...

Nature Reviews Genetics, Published online: 11 April 2025; doi:10.1038/s41576-025-00836-zPost-transcriptional splicing has emerged as a key layer of gene expression regulation but is challenging to differentiate from co-transcriptional splicing. The authors review methodologies to detect post-transcriptional splicing, the mechanisms controlling splicing timing and how the temporal regulation of splicing affects gene expression.

Kirio et al. demonstrate that circRNAs steadily accumulate in the aging fly brain due to their exceptional stability. Temperature exposure (29°C) further elevates select circRNAs, which remain high weeks later, underscoring their potential as markers of both age and past experiences.

Combined expression of a mitochondrially targeted endonuclease and end-joining machinery enables formation of mtDNA deletions with a broad range of heteroplasmy.