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duderstadtlab.bsky.social
@duderstadtlab.bsky.social
In our lab, hardcore biochemistry meets physics. And coffee. We are investigating DNA replication through single molecule loupe @ Technical University of Munich and MPI of Biochemistry
Reposted
Thrilled to announce some big news!

Excited to share that I’ve been awarded a Wellcome Trust Early Career Award to establish my research as a Group Leader in the Glycosciences Program at the Institute of Biology and Experimental Medicine (IBYME) in Buenos Aires, Argentina, beginning in early 2026.
October 6, 2025 at 6:24 AM
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I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
October 4, 2025 at 11:50 AM
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Great work from Michelle Wang’s group at Cornell exploring how RNAP II and associated R-loops act as intrinsic roadblocks during mechanical DNA unzipping. These findings have mechanistic implications for transcription–replication conflict.

rdcu.be/eILVd
RNA polymerase II is a polar roadblock to a progressing DNA fork
Nature Communications - Transcription–replication conflicts can threaten genome stability. Here, the authors show that RNA polymerase II (Pol II) is a stronger roadblock to a DNA fork in the...
rdcu.be
September 30, 2025 at 12:10 AM
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When I was at NIH and shutdowns loomed, it was discovered that the administration was planning to shut down PubMed. The head of NCBI called the powers that be and pointed out that this would threaten patient safety

Who knows what will happen now

Just in case, remember Euruope-PMC
(europepmc.org )
a cartoon of donald trump with the words be prepared above him
ALT: a cartoon of donald trump with the words be prepared above him
media.tenor.com
September 29, 2025 at 1:35 PM
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Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...

With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:

Wadjet—Keeping a watchful eye on circular DNA.

www.cell.com/structure/fu...
September 5, 2025 at 6:29 AM
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🚨 2 × PhD positions @EPFL! 🚨
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → fschueder@ethz.ch
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT
July 31, 2025 at 8:56 AM
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Now peer reviewed and in its final form @natcomms.nature.com

www.nature.com/articles/s41...
July 21, 2025 at 12:59 PM
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Less than two weeks left to apply!

Postdoc positions in my lab to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists & structural biologists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...
Postdoctoral Fellow - Costa Lab
Salary for this Role: From £45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...
crick.wd3.myworkdayjobs.com
July 21, 2025 at 2:57 PM
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Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!
Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications
A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...
www.nature.com
July 21, 2025 at 10:53 AM
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Very exciting new technology manuscript from @alicepyne.bsky.social lab!
High-res AFM + AI pipeline quantifies complex DNA topology. t.co/qxD7o7ASl6
Very happy to contribute with @singlemoleculenev.bsky.social plasmids replicating past barriers in Xenopus egg extract.
Congratulation everyone!
https://doi.org/10.1038/s41467-025-60559-x
t.co
July 11, 2025 at 12:18 PM
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#pictureofthemonth: Illustration of the read-write mechanism by which ubiquitin tags are placed on histone H2A along genes to influence their expression. More: www.biochem.mpg.de/painting-gen...

July – institute calendar of @mpibiochem.bsky.social and @mpiforbi.bsky.social

Image by Tamara Bäßler
July 7, 2025 at 9:15 AM
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Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...
July 2, 2025 at 5:01 PM
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🥳Amazing publication from the Hornung lab, in collab. with the Carell lab, on the innate immune system: how modified RNA tricks the immune system. Big shout-out to the two first authors, Marleen Bérouti and Mirko Wagner! www.cell.com/cell/fulltex...
@v-hornung.bsky.social @m-berouti.bsky.social
Innate immune system: How modified RNA tricks the immune system
Researchers at LMU have elucidated why certain RNA modifications do not trigger an immune response—a key mechanism for RNA therapeutics.
www.lmu.de
July 1, 2025 at 12:17 PM
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It also has devastating cuts to virtually everything I want my taxes to support: science, a safety net, conservation, etc. Call your Senator today--30 seconds will work. Tell them to vote NO on this bill. Tillis is at: (202) 224-6342 3/n
June 29, 2025 at 2:52 PM
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The proposed budget slashes NIH by ~40%, devastating medical research, clinical trials, treatment breakthroughs—life-saving work.

Thousands of lives at risk for tax cuts for the rich? NO THANKS.

Tell your senators to oppose the cuts HERE:
zurl.co/qiKdX


zurl.co/RCR0y
#CutsCostLives
Will the Senate Save NIH?
Rescuing biomedical research is every bit as important as saving Medicaid.
zurl.co
June 29, 2025 at 1:57 PM
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new preprint alert from the lab: bringing some order into the wide and wild diversity of archaeal histones. Who has acidic histones? who has multiple histones? what histone combinations do archaea employ and why? find all of this and more here
www.biorxiv.org/content/10.1...
June 27, 2025 at 4:01 PM
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Postdoc positions open in my lab (Francis Crick Institute) to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...
June 20, 2025 at 9:38 AM
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This is a piece that I and @karsten-rippe.bsky.social discussing a lot, and a topic that is very close to my heart. The editors @naturerevgenet.bsky.social gave us the stage to do so, and the final version of our review is now available under this link: rdcu.be/erP1u

A short thread follows 1/n
June 19, 2025 at 4:37 PM
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Latest #CDlab paper online now:
Cohesin supercoils DNA during loop extrusion
www.cell.com/cell-reports...

This extensive study was led by Jan Michael Peters at Vienna; our lab contributed mostly modeling of plasmid supercoiling (@romanbarth.bsky.social‬) and single-molecule data (Richard Janissen).
June 16, 2025 at 12:01 PM
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We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
May 26, 2025 at 4:56 PM
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Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established.
www.science.org/doi/10.1126/...
Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes
Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.
www.science.org
May 21, 2025 at 6:59 PM
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Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...

@mpi-bio-fml.bsky.social
May 12, 2025 at 9:17 AM
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Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
May 7, 2025 at 2:56 PM
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📢Stigler lab in collaboration with the Holt lab decipher that LINE-1 ORF1p forms super-saturated co-condensates with RNA that enable DNA binding and nuclear entry during mitosis.
Congratulations!👏👏👏
Details: www.science.org/doi/10.1126/...
LINE-1 ribonucleoprotein condensates bind DNA to enable nuclear entry during mitosis
ORF1p forms condensates that bind to chromosomes during cell division, allowing L1 elements to insert themselves into the genome.
www.science.org
May 6, 2025 at 7:02 AM