Dirk Remus
@dirkremus.bsky.social
PI @ Sloan Kettering Institute, New York | DNA replication | https://www.mskcc.org/research/ski/labs/dirk-remus | Co-Director, BCMB PhD program, Weill Cornell Medicine| https://gradschool.weill.cornell.edu/bcmb-allied-program | Views are my own.
Reposted by Dirk Remus
Hi all!
We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids.
www.biorxiv.org/content/10.6...
#3DGenome #chromatin #Cohesin #centromere
We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids.
www.biorxiv.org/content/10.6...
#3DGenome #chromatin #Cohesin #centromere
CAD-C reveals centromere pairing and near-perfect alignment of sister chromatids
Three-dimensional (3D) genome organization plays a central role in gene regulation, chromatin folding, and genome stability. Although chromosome-conformation capture (3C)-derived methods have revoluti...
www.biorxiv.org
December 20, 2025 at 4:43 PM
Hi all!
We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids.
www.biorxiv.org/content/10.6...
#3DGenome #chromatin #Cohesin #centromere
We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids.
www.biorxiv.org/content/10.6...
#3DGenome #chromatin #Cohesin #centromere
Reposted by Dirk Remus
@science.org Chromatin buffers torsional stress during transcription | Science www.science.org/doi/10.1126/...
Chromatin buffers torsional stress during transcription
During eukaryotic transcription, Pol II must overcome nucleosome obstacles and, because of DNA’s helical structure, must also rotate relative to DNA, generating torsional stress. However, there is lim...
www.science.org
December 19, 2025 at 10:31 PM
@science.org Chromatin buffers torsional stress during transcription | Science www.science.org/doi/10.1126/...
Reposted by Dirk Remus
Hi all !
Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding.
We will investigate the role the 3D chromatin organization in Replication and Repair.
www.ens-lyon.fr/LBMC/equipes...
Job offers out soon!
#NewPI #3DGenome #chromatin #replication
Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding.
We will investigate the role the 3D chromatin organization in Replication and Repair.
www.ens-lyon.fr/LBMC/equipes...
Job offers out soon!
#NewPI #3DGenome #chromatin #replication
Chromatin Architecture in Replication and Repair (CARR) — Laboratoire de Biologie et Modélisation de la Cellule
Personal tools
www.ens-lyon.fr
December 17, 2025 at 8:13 AM
Hi all !
Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding.
We will investigate the role the 3D chromatin organization in Replication and Repair.
www.ens-lyon.fr/LBMC/equipes...
Job offers out soon!
#NewPI #3DGenome #chromatin #replication
Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding.
We will investigate the role the 3D chromatin organization in Replication and Repair.
www.ens-lyon.fr/LBMC/equipes...
Job offers out soon!
#NewPI #3DGenome #chromatin #replication
Reposted by Dirk Remus
please look here for more information about a postdoc position careers.mskcc.org/vacancies/93...
93229-Postdoctoral Researcher – Laboratory of Max Wilkinson | Memorial Sloan Kettering Cancer Center
careers.mskcc.org
October 31, 2025 at 7:12 PM
please look here for more information about a postdoc position careers.mskcc.org/vacancies/93...
Reposted by Dirk Remus
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
Wilkinson Lab
We discover and study reverse transcriptases
wilkinsonlab.bio
October 31, 2025 at 7:00 PM
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
Great work from Michelle Wang’s group at Cornell exploring how RNAP II and associated R-loops act as intrinsic roadblocks during mechanical DNA unzipping. These findings have mechanistic implications for transcription–replication conflict.
rdcu.be/eILVd
rdcu.be/eILVd
RNA polymerase II is a polar roadblock to a progressing DNA fork
Nature Communications - Transcription–replication conflicts can threaten genome stability. Here, the authors show that RNA polymerase II (Pol II) is a stronger roadblock to a DNA fork in the...
rdcu.be
September 30, 2025 at 12:10 AM
Great work from Michelle Wang’s group at Cornell exploring how RNAP II and associated R-loops act as intrinsic roadblocks during mechanical DNA unzipping. These findings have mechanistic implications for transcription–replication conflict.
rdcu.be/eILVd
rdcu.be/eILVd
Reposted by Dirk Remus
Congrats Maria! very well deserved... @mskcancercenter.bsky.social @mskeducation.bsky.social
Maria Jasin, whose fundamental research on repair of damaged DNA in cells has transformed our understanding of cancers linked to inherited gene mutations, has been selected as the 2025 recipient of the Pearl Meister Greengard Prize. Congratulations! #WomenInScience
Developmental biologist Maria Jasin wins the 2025 Pearl Meister Greengard Prize - News
Maria Jasin, whose fundamental research on repair of damaged DNA in cells has transformed our understanding of cancers linked to inherited gene mutations, has been selected as the 2025 recipient of the Pearl Meister Greengard Prize. Awarded annually by ...
www.rockefeller.edu
September 17, 2025 at 2:29 PM
Congrats Maria! very well deserved... @mskcancercenter.bsky.social @mskeducation.bsky.social
Reposted by Dirk Remus
My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social
DNA2 enables growth by restricting recombination-restarted replication - Nature
DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...
www.nature.com
September 3, 2025 at 3:59 PM
My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social
Reposted by Dirk Remus
Just out: BRCA2 reversion mutation–independent resistance to PARP inhibition through impaired DNA prereplication complex function | PNAS www.pnas.org/doi/10.1073/... @mskcancercenter.bsky.social @kyriepappas.bsky.social #SawyersLabMSKCC
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
June 10, 2025 at 3:53 PM
Just out: BRCA2 reversion mutation–independent resistance to PARP inhibition through impaired DNA prereplication complex function | PNAS www.pnas.org/doi/10.1073/... @mskcancercenter.bsky.social @kyriepappas.bsky.social #SawyersLabMSKCC
Reposted by Dirk Remus
If you have not already, make sure to check out two fantastic recent preprints from the Patel and Wang labs on the same topic investigated using magnetic tweezers and angular optical trapping:
doi.org/10.1101/2024... and doi.org/10.1101/2024...
doi.org/10.1101/2024... and doi.org/10.1101/2024...
Torsion is a Dynamic Regulator of DNA Replication Stalling and Reactivation
The inherent helical structure of DNA dictates that a replisome must rotate relative to DNA during replication, presenting inevitable topological challenges to replication. However, little is known ab...
doi.org
April 9, 2025 at 11:04 AM
If you have not already, make sure to check out two fantastic recent preprints from the Patel and Wang labs on the same topic investigated using magnetic tweezers and angular optical trapping:
doi.org/10.1101/2024... and doi.org/10.1101/2024...
doi.org/10.1101/2024... and doi.org/10.1101/2024...
Reposted by Dirk Remus
🚨New preprint🚨We developed a transverse flow imaging approach to directly visualize bacterial replisomes challenged by topological barriers and discovered a fast rotational mode of replisome operation. Fantastic work by thomasmretzer.bsky.social and Hamdan Lab! tinyurl.com/replisomerot...
Real-time imaging of rotation during synthesis by the replisome
During chromosome replication, unwinding by the helicase and synthesis by the polymerases can lead to overwinding and supercoiling of DNA. The mechanical consequences of these events and resulting loc...
tinyurl.com
April 9, 2025 at 11:04 AM
🚨New preprint🚨We developed a transverse flow imaging approach to directly visualize bacterial replisomes challenged by topological barriers and discovered a fast rotational mode of replisome operation. Fantastic work by thomasmretzer.bsky.social and Hamdan Lab! tinyurl.com/replisomerot...
Reposted by Dirk Remus
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
April 6, 2025 at 9:45 AM
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
Reposted by Dirk Remus
Our work on the regulation is out www.cell.com/stem-cell-re...! Congrats to our collaborators in the Vierbuchen and Mezey labs. It turns out that genetic variation has to be very much considered if you want to harness stem cells safely in biomedicine. #pluripotency #epigenetics #stemcells
Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells
Pluripotent stem cells are susceptible to acquiring detrimental epigenetic abnormalities,
posing a hurdle to their safe use in biomedical applications. Using pluripotent cells
with defined genetic bac...
www.cell.com
March 13, 2025 at 8:41 PM
Our work on the regulation is out www.cell.com/stem-cell-re...! Congrats to our collaborators in the Vierbuchen and Mezey labs. It turns out that genetic variation has to be very much considered if you want to harness stem cells safely in biomedicine. #pluripotency #epigenetics #stemcells
Reposted by Dirk Remus
Combining bacterial end joining with programmable nucleases, we induced mtDNA deletions in human cells at varying heteroplasmy and examined their metabolic and cellular effects. Simple tool to apply across any cell type and engineer pathogenic mtDNA deletions. www.sciencedirect.com/science/arti...
Engineering mtDNA deletions by reconstituting end joining in human mitochondria
Recent breakthroughs in the genetic manipulation of mitochondrial DNA (mtDNA) have enabled precise base substitutions and the efficient elimination of…
www.sciencedirect.com
March 10, 2025 at 6:38 PM
Combining bacterial end joining with programmable nucleases, we induced mtDNA deletions in human cells at varying heteroplasmy and examined their metabolic and cellular effects. Simple tool to apply across any cell type and engineer pathogenic mtDNA deletions. www.sciencedirect.com/science/arti...
Shout out to Sloan Kettering @mskcancercenter.bsky.social for supporting basic science / foundational biology...
www.mskcc.org/news/cryo-el...
www.mskcc.org/news/cryo-el...
Cryo-Electron Microscopy Reveals Hidden Mechanics of DNA Replication, Sheds New Light on Cancer Target
MSK researchers shed new light on G-quadruplexes, a type of secondary DNA structure that has attracted attention as a potential therapeutic target in cancer.
www.mskcc.org
March 7, 2025 at 4:33 PM
Shout out to Sloan Kettering @mskcancercenter.bsky.social for supporting basic science / foundational biology...
www.mskcc.org/news/cryo-el...
www.mskcc.org/news/cryo-el...
Reposted by Dirk Remus
In our latest preprint, we uncover how CMG helicase overcomes covalent DNA-protein crosslinks. Check out our findings here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
March 7, 2025 at 12:05 PM
In our latest preprint, we uncover how CMG helicase overcomes covalent DNA-protein crosslinks. Check out our findings here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Dirk Remus
Thrilled to share first part of my post-doc work, published today in @science.org www.science.org/doi/10.1126/...
We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.
We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
www.science.org
March 6, 2025 at 11:54 PM
Thrilled to share first part of my post-doc work, published today in @science.org www.science.org/doi/10.1126/...
We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.
We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.
How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
www.science.org
March 6, 2025 at 9:30 PM
How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!
Reposted by Dirk Remus
I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
www.science.org
March 6, 2025 at 7:40 PM
I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Dirk Remus
MSK has been recognized as one of the national's top workplaces for inclusion and diversity by @newsweek.com magazine. 🎉
MSK earned five stars, the highest ranking, among mid to large employers in America. Learn more: www.newsweek.com/rankings/ame...
MSK earned five stars, the highest ranking, among mid to large employers in America. Learn more: www.newsweek.com/rankings/ame...
America's Greatest Workplaces for Inclusion & Diversity 2025
Newsweek is partnering with Plant-A Insights Group to honor America's Greatest Workplaces for Inclusion & Diversity 2025. These companies are committed to fostering an inclusive and diverse work envir...
www.newsweek.com
March 4, 2025 at 6:36 PM
MSK has been recognized as one of the national's top workplaces for inclusion and diversity by @newsweek.com magazine. 🎉
MSK earned five stars, the highest ranking, among mid to large employers in America. Learn more: www.newsweek.com/rankings/ame...
MSK earned five stars, the highest ranking, among mid to large employers in America. Learn more: www.newsweek.com/rankings/ame...
Reposted by Dirk Remus
In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨
🚨 New preprint alert! 🚨 doi.org/10.1101/2025...
🧵👇 (1/)
🚨 New preprint alert! 🚨 doi.org/10.1101/2025...
🧵👇 (1/)
doi.org
February 27, 2025 at 3:15 AM
In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨
🚨 New preprint alert! 🚨 doi.org/10.1101/2025...
🧵👇 (1/)
🚨 New preprint alert! 🚨 doi.org/10.1101/2025...
🧵👇 (1/)
Reposted by Dirk Remus
Proud to share our latest publication in @narjournal.bsky.social! A fantastic team effort led by @marcelwerner.bsky.social and Manuel T., where we explore the chromatin dynamics at transcription-replication conflicts (TRC) sites and their impact on genome stability using a novel TRC reporter system.
I am delighted to share the main work of my PhD project in the @hamperllab.bsky.social now out in @narjournal.bsky.social. TRCs drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery.
academic.oup.com/nar/article/...
#TRC #r-loop #chromatin
More below 👇
academic.oup.com/nar/article/...
#TRC #r-loop #chromatin
More below 👇
Transcription-replication conflicts drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery
Abstract. Progressing transcription and replication machineries profoundly impact their underlying chromatin template. Consequently, transcription-replicat
academic.oup.com
February 20, 2025 at 3:35 PM
Proud to share our latest publication in @narjournal.bsky.social! A fantastic team effort led by @marcelwerner.bsky.social and Manuel T., where we explore the chromatin dynamics at transcription-replication conflicts (TRC) sites and their impact on genome stability using a novel TRC reporter system.
Reposted by Dirk Remus
Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
February 17, 2025 at 11:04 AM
Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Dirk Remus
Setting up shop in advance of closing our profile on X.
February 13, 2025 at 4:16 PM
Setting up shop in advance of closing our profile on X.