Chloé Roffay
@chloeroffay.bsky.social
From physics to cells to gastruloids • Postdoc in @dianapinheiro.bsky.social • PhD : @rouxlab.bsky.social 🔬🐬 🐈⬛🐈⬛ 🎨 • alum cpes @psl-univ.bsky.social • she/her • Views my own
Reposted by Chloé Roffay
Launching celldynamicslab.com, the homepage of our new group at EPFL working on cell fragmentation, membrane and cortex mechanics, FLIM imaging, and microfluidics tools. MSc/PhD or postdocs interested in quantitative cell biology are welcome to reach out. We're also hiring a lab manager in 2026!
Main - Cell Dynamics and Fragmentation Lab
The Cell Dynamics and Fragmentation lab at We study how single cells move and fragment in complex environments using microfluidics. We bring tools and concep...
celldynamicslab.com
November 11, 2025 at 5:10 PM
Launching celldynamicslab.com, the homepage of our new group at EPFL working on cell fragmentation, membrane and cortex mechanics, FLIM imaging, and microfluidics tools. MSc/PhD or postdocs interested in quantitative cell biology are welcome to reach out. We're also hiring a lab manager in 2026!
Reposted by Chloé Roffay
Happy to share our new work from my graduate lab, with friends & colleagues @suewop.bsky.social, Pieter Derksen, Gary Han, Nikolas Claussen, and @streichan.bsky.social! We find that germ layers exhibit persistent, distinct flow patterns during zebrafish gastrulation.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Stationary and germ layer-specific cellular flows shape the zebrafish gastrula
During gastrulation, a sequence of complex processes transforms the blastula into a multilayered embryo. Fixed sample analysis has revealed much about how genetic signaling cascades determine the majo...
www.biorxiv.org
November 13, 2025 at 9:17 PM
Happy to share our new work from my graduate lab, with friends & colleagues @suewop.bsky.social, Pieter Derksen, Gary Han, Nikolas Claussen, and @streichan.bsky.social! We find that germ layers exhibit persistent, distinct flow patterns during zebrafish gastrulation.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Chloé Roffay
Cell type-independent timekeeping gene modules enable embryonic stage prediction in zebrafish https://www.biorxiv.org/content/10.1101/2025.11.12.688039v1
November 13, 2025 at 8:34 PM
Cell type-independent timekeeping gene modules enable embryonic stage prediction in zebrafish https://www.biorxiv.org/content/10.1101/2025.11.12.688039v1
Reposted by Chloé Roffay
We are highlighting Austin Smith, a @biologists.bsky.social Director, @dmmjournal.bsky.social Guest Editor and former @dev-journal.bsky.social Editor, as an extraordinary biologist. #100biologists
@ausmith.bsky.social
@ausmith.bsky.social
November 3, 2025 at 3:49 PM
We are highlighting Austin Smith, a @biologists.bsky.social Director, @dmmjournal.bsky.social Guest Editor and former @dev-journal.bsky.social Editor, as an extraordinary biologist. #100biologists
@ausmith.bsky.social
@ausmith.bsky.social
'AI' is based on training dataset. Dataset are annotated by humans.
This week's @nature.com cover highlights a report of A.I.- mediated distortion
When #ChatGPT was asked to rate 40,000 résumés, it ranked the older male candidates as better quality than the younger female applicants nature.com/articles/s41...
When #ChatGPT was asked to rate 40,000 résumés, it ranked the older male candidates as better quality than the younger female applicants nature.com/articles/s41...
November 3, 2025 at 6:47 AM
'AI' is based on training dataset. Dataset are annotated by humans.
Reposted by Chloé Roffay
And another great gut epithelium story from our cool neighbors !!!😎 @cbitoulouse.bsky.social
Ever wondered how adult organs preserve epithelial barrier integrity while continuously exposed to mechanical stress? We tackled this question in our new preprint – led by our brilliant PhD student Vishnu Krishnakumar! (1/11)
🔗 www.biorxiv.org/content/10.1...
🔗 www.biorxiv.org/content/10.1...
Robust pan-junctional reinforcement preserves the gut epithelial barrier under mechanical stress
Epithelia are specialized tissue barriers that safeguard the organism's internal milieu from the hostile external environment, a function critically dependent on intercellular junctions. In the colon,...
www.biorxiv.org
October 30, 2025 at 2:48 PM
And another great gut epithelium story from our cool neighbors !!!😎 @cbitoulouse.bsky.social
Reposted by Chloé Roffay
Our ForSys method to infer the mechanical state of a tissue in space and time is published now in @cp-iscience.bsky.social !
www.cell.com/iscience/ful...
(1/10)
www.cell.com/iscience/ful...
(1/10)
October 20, 2025 at 1:03 PM
Our ForSys method to infer the mechanical state of a tissue in space and time is published now in @cp-iscience.bsky.social !
www.cell.com/iscience/ful...
(1/10)
www.cell.com/iscience/ful...
(1/10)
Reposted by Chloé Roffay
Zebrafish embryo undergoing gastrulation. Credit to Dr. Gopi Shah at EMBL. #ZebrafishZunday 🧪
October 19, 2025 at 9:24 AM
Zebrafish embryo undergoing gastrulation. Credit to Dr. Gopi Shah at EMBL. #ZebrafishZunday 🧪
Reposted by Chloé Roffay
A lot of pressure on the different grant schemes, and pretty much independent of career stage.
October 17, 2025 at 10:20 AM
A lot of pressure on the different grant schemes, and pretty much independent of career stage.
Reposted by Chloé Roffay
October 17, 2025 at 9:22 AM
Reposted by Chloé Roffay
q.e.d Science
Critical Thinking AI for constructive criticism and science evaluation
qedscience.com
October 15, 2025 at 12:22 PM
Reposted by Chloé Roffay
New preprint from the lab! We discovered a transient fluidization in the basal region of human forebrains by tracking microdroplets in cerebral organoids.This “basal fluidization”, absent in gorilla and mouse, may contribute to greater surface expansion in human forebrains
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doi.org/10.1101/2025...
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doi.org/10.1101/2025...
October 8, 2025 at 1:05 PM
New preprint from the lab! We discovered a transient fluidization in the basal region of human forebrains by tracking microdroplets in cerebral organoids.This “basal fluidization”, absent in gorilla and mouse, may contribute to greater surface expansion in human forebrains
1/
doi.org/10.1101/2025...
1/
doi.org/10.1101/2025...
Reposted by Chloé Roffay
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 5, 2025 at 4:04 PM
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Reposted by Chloé Roffay
First, check out my recent bioRxiv preprint w/ @oweinerlab.bsky.social: www.biorxiv.org/content/10.1...
We find contractility shifts the proportion of implantation-competent embryos from young and aged females. Keep reading this for more info!
We find contractility shifts the proportion of implantation-competent embryos from young and aged females. Keep reading this for more info!
A mechanical origin for implantation defects in embryos from aged females
Women over 35 experience a marked reduction in fertility. The origin of these fertility defects appears to reside in the implantation capacity of the embryo itself, but the mechanistic basis of this i...
www.biorxiv.org
October 1, 2025 at 6:21 PM
First, check out my recent bioRxiv preprint w/ @oweinerlab.bsky.social: www.biorxiv.org/content/10.1...
We find contractility shifts the proportion of implantation-competent embryos from young and aged females. Keep reading this for more info!
We find contractility shifts the proportion of implantation-competent embryos from young and aged females. Keep reading this for more info!
Reposted by Chloé Roffay
I have a PhD student and an undergrad student positions open at the Institute of Biotechnology @helsinki.fi We are using reconstitution, cell and structural biology to study protein-membrane interactions and membrane microdomain formation. Links to calls below! Feel free to drop a message and share!
September 29, 2025 at 3:45 PM
I have a PhD student and an undergrad student positions open at the Institute of Biotechnology @helsinki.fi We are using reconstitution, cell and structural biology to study protein-membrane interactions and membrane microdomain formation. Links to calls below! Feel free to drop a message and share!
Reposted by Chloé Roffay
I’m not sure as a community we **truely** acknowledged how powerful the #CellMigrationSeminarSeries series was at keeping us connected at such an uncertain time. Thank-you @ankita13jha.bsky.social @adamshellard.bsky.social @drjenmitchell.bsky.social & @simplythebex.bsky.social
September 29, 2025 at 2:07 AM
I’m not sure as a community we **truely** acknowledged how powerful the #CellMigrationSeminarSeries series was at keeping us connected at such an uncertain time. Thank-you @ankita13jha.bsky.social @adamshellard.bsky.social @drjenmitchell.bsky.social & @simplythebex.bsky.social
Reposted by Chloé Roffay
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM https://www.biorxiv.org/content/10.1101/2025.09.23.678124v1
September 26, 2025 at 2:49 AM
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM https://www.biorxiv.org/content/10.1101/2025.09.23.678124v1
Reposted by Chloé Roffay
🧵1/14 Preprint thread! Can we predict a cell’s fate based on its dynamics? 🔮 Our new study unveils a framework for watching development unfold in real-time, revealing how a cell's shape and movement encode info about its future fate. 🔬📄 Preprint: tinyurl.com/4shf8v4x
September 22, 2025 at 9:37 AM
🧵1/14 Preprint thread! Can we predict a cell’s fate based on its dynamics? 🔮 Our new study unveils a framework for watching development unfold in real-time, revealing how a cell's shape and movement encode info about its future fate. 🔬📄 Preprint: tinyurl.com/4shf8v4x
Reposted by Chloé Roffay
💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
open-research-europe.ec.europa.eu
September 20, 2025 at 2:41 PM
💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
Reposted by Chloé Roffay
Are digits modified fins, or evolutionary innovations? Read how we tackled this old question from a new angle🧪
A story with @chasebolt.bsky.social, @homeobox.bsky.social and myself, coordinated by @denisduboule.bsky.social from @college-de-france.fr and published in @nature.com today!
#InHoxWeTrust
A story with @chasebolt.bsky.social, @homeobox.bsky.social and myself, coordinated by @denisduboule.bsky.social from @college-de-france.fr and published in @nature.com today!
#InHoxWeTrust
September 17, 2025 at 5:26 PM
Are digits modified fins, or evolutionary innovations? Read how we tackled this old question from a new angle🧪
A story with @chasebolt.bsky.social, @homeobox.bsky.social and myself, coordinated by @denisduboule.bsky.social from @college-de-france.fr and published in @nature.com today!
#InHoxWeTrust
A story with @chasebolt.bsky.social, @homeobox.bsky.social and myself, coordinated by @denisduboule.bsky.social from @college-de-france.fr and published in @nature.com today!
#InHoxWeTrust
Reposted by Chloé Roffay
Be our new colleague! 🎉
The Dept of Molecular and Cellular Biology at the University of Geneva is hiring an Assistant Professor or Associate Professor.
👉 More details here: lnkd.in/e8Z9eAm4
👉 Our department: mocel.unige.ch
Please feel free to share this opportunity with your network.
The Dept of Molecular and Cellular Biology at the University of Geneva is hiring an Assistant Professor or Associate Professor.
👉 More details here: lnkd.in/e8Z9eAm4
👉 Our department: mocel.unige.ch
Please feel free to share this opportunity with your network.
September 15, 2025 at 2:33 PM
Be our new colleague! 🎉
The Dept of Molecular and Cellular Biology at the University of Geneva is hiring an Assistant Professor or Associate Professor.
👉 More details here: lnkd.in/e8Z9eAm4
👉 Our department: mocel.unige.ch
Please feel free to share this opportunity with your network.
The Dept of Molecular and Cellular Biology at the University of Geneva is hiring an Assistant Professor or Associate Professor.
👉 More details here: lnkd.in/e8Z9eAm4
👉 Our department: mocel.unige.ch
Please feel free to share this opportunity with your network.
Reposted by Chloé Roffay
@fritzlaylin.bsky.social et al. propose a simple way to highlight both experimental #reproducibility & cell-to-cell variation, while avoiding pitfalls common in analysis of cell biology data rupress.org/jcb/article/...
📕 Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...
📕 Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...
September 12, 2025 at 2:03 PM
@fritzlaylin.bsky.social et al. propose a simple way to highlight both experimental #reproducibility & cell-to-cell variation, while avoiding pitfalls common in analysis of cell biology data rupress.org/jcb/article/...
📕 Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...
📕 Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...
Reposted by Chloé Roffay
Preprint alert!
We combined microfluidics, proteomics, and RNA-seq to map spatiotemporal protein expression during mouse somitogenesis and found a novel regulatory strategy: dynamic antagonistic gradients fine-tune signalling strength.
doi.org/10.1101/2025...
We combined microfluidics, proteomics, and RNA-seq to map spatiotemporal protein expression during mouse somitogenesis and found a novel regulatory strategy: dynamic antagonistic gradients fine-tune signalling strength.
doi.org/10.1101/2025...
September 9, 2025 at 4:05 PM
Preprint alert!
We combined microfluidics, proteomics, and RNA-seq to map spatiotemporal protein expression during mouse somitogenesis and found a novel regulatory strategy: dynamic antagonistic gradients fine-tune signalling strength.
doi.org/10.1101/2025...
We combined microfluidics, proteomics, and RNA-seq to map spatiotemporal protein expression during mouse somitogenesis and found a novel regulatory strategy: dynamic antagonistic gradients fine-tune signalling strength.
doi.org/10.1101/2025...
Reposted by Chloé Roffay
Wnt signaling modulates tissue mechanics, actin order, and regeneration in Hydra vulgaris https://www.biorxiv.org/content/10.1101/2025.09.02.673719v1
September 6, 2025 at 2:48 AM
Wnt signaling modulates tissue mechanics, actin order, and regeneration in Hydra vulgaris https://www.biorxiv.org/content/10.1101/2025.09.02.673719v1
Reposted by Chloé Roffay
For decades, living embryos were the favourite tool to study development. However, their complexity hindered precise dissection of many processes. This comprehensive review provides a comparison between embryos and the power of gastruloids, opening up a new wave of dev bio.
doi.org/10.1016/j.cd...
doi.org/10.1016/j.cd...
September 5, 2025 at 5:30 PM
For decades, living embryos were the favourite tool to study development. However, their complexity hindered precise dissection of many processes. This comprehensive review provides a comparison between embryos and the power of gastruloids, opening up a new wave of dev bio.
doi.org/10.1016/j.cd...
doi.org/10.1016/j.cd...