@zhifei-fu0326.bsky.social
Reposted
Do you like following rules… but also breaking them?
So does your dopaminergic synapse! 😎🧠
Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).
So does your dopaminergic synapse! 😎🧠
Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).
October 15, 2025 at 1:46 PM
Do you like following rules… but also breaking them?
So does your dopaminergic synapse! 😎🧠
Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).
So does your dopaminergic synapse! 😎🧠
Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).
Reposted
How cool!!!
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric green fluorescent protein recently
monomerised through sequence engi...
www.jbc.org
October 22, 2025 at 8:53 PM
How cool!!!
Reposted
What an amazing opportunity to do impactful work in bioimage analysis!!!!
Job alert!
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
Research engineer in Bioimage Analysis for the researchers of the inIdEx FORMULA
Hi all We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? The position is in the Instit...
forum.image.sc
October 7, 2025 at 1:19 PM
What an amazing opportunity to do impactful work in bioimage analysis!!!!
Reposted
What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
October 7, 2025 at 3:08 PM
What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
Reposted
This Protocol results from the Joint Undertaking for Morphological Profiling Cell Painting Consortium, and improves the Cell Painting assay by quantitatively detecting morphological phenotypes, improving the ability to group perturbations with similar behavior.
Optimizing the Cell Painting assay for image-based profiling
Nature Protocols - We provide an updated protocol for image-based profiling with Cell Painting. A detailed procedure, with standardized conditions for the assay, is presented, along with a...
rdcu.be
October 8, 2025 at 8:20 AM
This Protocol results from the Joint Undertaking for Morphological Profiling Cell Painting Consortium, and improves the Cell Painting assay by quantitatively detecting morphological phenotypes, improving the ability to group perturbations with similar behavior.
Reposted
I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
October 4, 2025 at 11:50 AM
I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
Reposted
Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...
October 2, 2025 at 6:57 PM
Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...
Reposted
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
September 26, 2025 at 6:14 AM
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
Reposted
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains
A family of rationally designed time-resolved fluorescent proteins with controllable
lifetimes across the visible spectrum enables simultaneous multiplexed live imaging,
super-resolution microscopy, a...
www.cell.com
September 26, 2025 at 8:07 AM
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
Is it impressive? Yes
Is it new? No
Should you use it? Probably not.
www.cell.com/cell/fulltex...
Reposted
Genetically Encoded Death Indicator (GEDI): a live cell biosensor protocol for high-resolution screening of therapy-resistant cancer cells by Jessica S Blackburn and team: www.biorxiv.org/content/10.1...
September 26, 2025 at 1:24 PM
Genetically Encoded Death Indicator (GEDI): a live cell biosensor protocol for high-resolution screening of therapy-resistant cancer cells by Jessica S Blackburn and team: www.biorxiv.org/content/10.1...
Reposted
Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites
Self-complementing bipartite fluorescent proteins (FPs) are useful tools for the detection of protein–protein proximity and for localizing fluorophores to membrane–membrane contact sites. Here, we rep...
pubs.acs.org
September 7, 2025 at 11:31 AM
Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Reposted
Looking for your new favorite red fluorescent protein? mScarlet3-H (aka mYongHong) is highly stable, monomeric, and bright - it may be just what you need!
https://twp.ai/4iqbT5
https://twp.ai/4iqbT5
August 25, 2025 at 1:30 PM
Looking for your new favorite red fluorescent protein? mScarlet3-H (aka mYongHong) is highly stable, monomeric, and bright - it may be just what you need!
https://twp.ai/4iqbT5
https://twp.ai/4iqbT5
Reposted
(Yet another) shout-out to @joachimgoedhart.bsky.social and colleagues for their ongoing technical service to the cell biology community – in this case, the yeast cell biology community. Special kudos for making all the key plasmids available on @addgene.bsky.social. Stellar work all around!
Side-by-side systematic characterization of novel FPs in budding yeast
Fluorescent proteins (FPs) have become indispensable for biological research. Since the discovery of the first FP, Aequorea victoria green fluorescent protein (avGFP), new fluorescent proteins are con...
doi.org
August 15, 2025 at 8:39 PM
(Yet another) shout-out to @joachimgoedhart.bsky.social and colleagues for their ongoing technical service to the cell biology community – in this case, the yeast cell biology community. Special kudos for making all the key plasmids available on @addgene.bsky.social. Stellar work all around!
The plasmids related to our mScarlet3-H are available on Addgene now. www.addgene.org/Zhifei_Fu/
August 16, 2025 at 2:47 AM
The plasmids related to our mScarlet3-H are available on Addgene now. www.addgene.org/Zhifei_Fu/
Reposted
Delighted to announce here that @ritastrack.bsky.social is the new Chief editor of Nature Biomedical Engineering. Congratulations Rita!
August 1, 2025 at 2:26 PM
Delighted to announce here that @ritastrack.bsky.social is the new Chief editor of Nature Biomedical Engineering. Congratulations Rita!
Reposted
💡 Always a good time to be reminded: "if you fuse your protein of interest to GFP or EGFP to study the protein's behavior ... you are using a tag with a serious drawback."
Same goes for anything that doesn't have 'm' in its name (looking at you, dTomato and StayGold)
Same goes for anything that doesn't have 'm' in its name (looking at you, dTomato and StayGold)
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.
blog.addgene.org
July 29, 2025 at 4:04 PM
💡 Always a good time to be reminded: "if you fuse your protein of interest to GFP or EGFP to study the protein's behavior ... you are using a tag with a serious drawback."
Same goes for anything that doesn't have 'm' in its name (looking at you, dTomato and StayGold)
Same goes for anything that doesn't have 'm' in its name (looking at you, dTomato and StayGold)
Reposted
Nature Methods is hiring!
We are seeking a new editor with expertise in microscopy, imaging technology, and/or cell biology. The position is available in NYC/Jersey City or Shanghai/Beijing on a hybrid working model.
Apply by August 3!
springernature.wd3.myworkdayjobs.com/SpringerNatu...
We are seeking a new editor with expertise in microscopy, imaging technology, and/or cell biology. The position is available in NYC/Jersey City or Shanghai/Beijing on a hybrid working model.
Apply by August 3!
springernature.wd3.myworkdayjobs.com/SpringerNatu...
a yellow sign that says we 're hiring
ALT: a yellow sign that says we 're hiring
media.tenor.com
July 9, 2025 at 5:58 PM
Nature Methods is hiring!
We are seeking a new editor with expertise in microscopy, imaging technology, and/or cell biology. The position is available in NYC/Jersey City or Shanghai/Beijing on a hybrid working model.
Apply by August 3!
springernature.wd3.myworkdayjobs.com/SpringerNatu...
We are seeking a new editor with expertise in microscopy, imaging technology, and/or cell biology. The position is available in NYC/Jersey City or Shanghai/Beijing on a hybrid working model.
Apply by August 3!
springernature.wd3.myworkdayjobs.com/SpringerNatu...
Reposted
Some graduate students, postdoctoral fellows and senior researchers choose a special type of science-focused summer, for instance at @mblscience.bsky.social and CSH Asia. @vivienm.bsky.social spoke with 23 people about their experiences. Here is the story. www.nature.com/articles/s41...
A sweet summer of science - Nature Methods
Some graduate students, postdoctoral fellows and senior researchers choose a special kind of science-focused summer.
www.nature.com
July 9, 2025 at 8:57 PM
Some graduate students, postdoctoral fellows and senior researchers choose a special type of science-focused summer, for instance at @mblscience.bsky.social and CSH Asia. @vivienm.bsky.social spoke with 23 people about their experiences. Here is the story. www.nature.com/articles/s41...
Reposted
Shared and not taken over by... Go for symbiosis!
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
A scientific future shared with AI
AI tools now exist to aid almost every aspect of the research process, from hypothesis generation and data analysis to manuscript drafting and publication. This Editorial examines what the future migh...
dx.plos.org
July 5, 2025 at 9:10 AM
Shared and not taken over by... Go for symbiosis!
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Reposted
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted
Our June issue is live!
www.nature.com/nmeth/volume...
On the cover, mScarlet3-H, a new monomeric red fluorescent protein. Image by Zhifei Fu. Paper here:
www.nature.com/articles/s41...
www.nature.com/nmeth/volume...
On the cover, mScarlet3-H, a new monomeric red fluorescent protein. Image by Zhifei Fu. Paper here:
www.nature.com/articles/s41...
June 16, 2025 at 3:33 PM
Our June issue is live!
www.nature.com/nmeth/volume...
On the cover, mScarlet3-H, a new monomeric red fluorescent protein. Image by Zhifei Fu. Paper here:
www.nature.com/articles/s41...
www.nature.com/nmeth/volume...
On the cover, mScarlet3-H, a new monomeric red fluorescent protein. Image by Zhifei Fu. Paper here:
www.nature.com/articles/s41...
Reposted
More nice talks at the Bioorganic GRC. Katharine Diehl tells us about pancACe (pronounced pancake) their genetically encoded acetyl-CoA sensor. www.cell.com/cell-chemica... 🥞
A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells
Smith et al. engineered a “turn on” fluorescent biosensor (dubbed “PancACe”) for acetyl-CoA
from a fusion of the proteins PanZ and circularly permuted GFP. PancACe measures changes
in acetyl-CoA level...
www.cell.com
June 18, 2025 at 11:47 PM
More nice talks at the Bioorganic GRC. Katharine Diehl tells us about pancACe (pronounced pancake) their genetically encoded acetyl-CoA sensor. www.cell.com/cell-chemica... 🥞
Reposted
Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress | Science Advances www.science.org/doi/10.1126/...
Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress
Quantitative imaging of the mitochondrial central dogma identifies translation hubs that are suppressed during stress.
www.science.org
April 18, 2025 at 10:39 PM
Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress | Science Advances www.science.org/doi/10.1126/...
Reposted
Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41...
April 21, 2025 at 11:48 PM
Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41...
go.nature.com/42maCP9 The story of the beginning of mScarlet3-H (aka mYongHong).
Research Communities by Springer Nature
Research Communities by Springer Nature provide a forum for all those interested in research to share the latest discoveries, news, and opinions.
go.nature.com
April 20, 2025 at 10:34 PM
go.nature.com/42maCP9 The story of the beginning of mScarlet3-H (aka mYongHong).