Ali Turk
@turkchembio.bsky.social
Chemical Biologist in the Schroeder lab at Cornell
Reposted by Ali Turk
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
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www.biorxiv.org/content/10.1...
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PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders
Proteins that bind to a target protein of interest, termed "binders," are essential components of biological research reagents and therapeutics. Target proteins present multiple binding surfaces with ...
www.biorxiv.org
November 19, 2025 at 6:10 PM
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
1/n
www.biorxiv.org/content/10.1...
1/n
Reposted by Ali Turk
www.nature.com/articles/s42...
Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency
@charlesmbrenner.bsky.social congratulations!
Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency
@charlesmbrenner.bsky.social congratulations!
Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency - Nature Metabolism
In a mouse model of the rare disease citrin deficiency, the authors discovered that the accumulation of glycerol-3-phosphate leads to ChREBP activation and FGF21 induction. The study identifies glycer...
www.nature.com
November 16, 2025 at 7:21 AM
www.nature.com/articles/s42...
Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency
@charlesmbrenner.bsky.social congratulations!
Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency
@charlesmbrenner.bsky.social congratulations!
Reposted by Ali Turk
Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications
Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, app...
www.nature.com
November 14, 2025 at 8:07 PM
Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ali Turk
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Proteome-wide in silico screening for human protein-protein interactions
Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...
biorxiv.org
November 12, 2025 at 9:26 PM
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Reposted by Ali Turk
www.nature.com/articles/s41...
Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy - Nature
Acetyl-coenzyme A functions as a non-canonical signal to trigger mitophagy, and the acetyl-coenzyme A–NLRX1 axis underlies the KRAS-inhibitor-induced mitophagy response and promotes drug resistan...
www.nature.com
November 12, 2025 at 6:02 PM
www.nature.com/articles/s41...
Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
Reposted by Ali Turk
My first (and first-author) paper investigating how Arabidopsis AUGMIN8 interacts with microtubules is now available online in @cytoskeljournal.bsky.social! I'm incredibly grateful to have performed this work as an undergrad and postbac in Sidney Shaw's lab at IUB. doi.org/10.1002/cm.7...
Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains
Plant cells create a plasma membrane-associated network of microtubules that are nucleated by γ-tubulin ring complexes primarily through microtubule-dependent microtubule nucleation (MDMN). This dyna...
doi.org
November 10, 2025 at 3:28 PM
My first (and first-author) paper investigating how Arabidopsis AUGMIN8 interacts with microtubules is now available online in @cytoskeljournal.bsky.social! I'm incredibly grateful to have performed this work as an undergrad and postbac in Sidney Shaw's lab at IUB. doi.org/10.1002/cm.7...
Reposted by Ali Turk
ORP5 controls the partitioning of phosphatidic acid between triacylglycerol and cardiolipin synthesis at mitochondria-ER-lipid droplet contact sites https://www.biorxiv.org/content/10.1101/2025.11.06.685814v1
November 7, 2025 at 10:30 PM
ORP5 controls the partitioning of phosphatidic acid between triacylglycerol and cardiolipin synthesis at mitochondria-ER-lipid droplet contact sites https://www.biorxiv.org/content/10.1101/2025.11.06.685814v1
Reposted by Ali Turk
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted by Ali Turk
Nature suggests you use their "Manuscript Adviser" bot to get advice before submitting
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
November 3, 2025 at 1:55 PM
Nature suggests you use their "Manuscript Adviser" bot to get advice before submitting
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
Reposted by Ali Turk
Excited to share my postdoc work @olzmannlab.bsky.social! We found lipid droplets, the cell’s lipid storage depots, are subject to oxidative damage and are protected by FSP1. Loss of FSP1 triggers droplet peroxidation and cell death, revealing a new layer of lipid quality control!
shorturl.at/B5XYD
shorturl.at/B5XYD
FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology
Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.
www.nature.com
October 29, 2025 at 8:45 PM
Excited to share my postdoc work @olzmannlab.bsky.social! We found lipid droplets, the cell’s lipid storage depots, are subject to oxidative damage and are protected by FSP1. Loss of FSP1 triggers droplet peroxidation and cell death, revealing a new layer of lipid quality control!
shorturl.at/B5XYD
shorturl.at/B5XYD
Reposted by Ali Turk
Molecular & Cellular Physiology (MCP) Monday
We are recruiting a tenure-track assistant professor to join the Dept of Molecular & Cellular Physiology at Stanford. Apply to be our colleague 1/n
facultypositions.stanford.edu/en-us/job/49...
We are recruiting a tenure-track assistant professor to join the Dept of Molecular & Cellular Physiology at Stanford. Apply to be our colleague 1/n
facultypositions.stanford.edu/en-us/job/49...
Stanford | Faculty Positions: Details - Assistant Professor of Molecular and Cellular Physiology
facultypositions.stanford.edu
October 27, 2025 at 11:33 PM
Molecular & Cellular Physiology (MCP) Monday
We are recruiting a tenure-track assistant professor to join the Dept of Molecular & Cellular Physiology at Stanford. Apply to be our colleague 1/n
facultypositions.stanford.edu/en-us/job/49...
We are recruiting a tenure-track assistant professor to join the Dept of Molecular & Cellular Physiology at Stanford. Apply to be our colleague 1/n
facultypositions.stanford.edu/en-us/job/49...
Reposted by Ali Turk
Excited to share our latest work out today in @cp-molcell.bsky.social, revealing a molecular link between succinate dehydrogenase and purine synthesis, connecting two fundamental metabolic pathways. Huge congratulations to the first author, Mushtaq Nengroo! www.sciencedirect.com/science/arti...
!
!
Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle
The de novo purine synthesis pathway is fundamental for nucleotide production, yet the role of mitochondrial metabolism in modulating this process rem…
www.sciencedirect.com
October 28, 2025 at 4:25 PM
Excited to share our latest work out today in @cp-molcell.bsky.social, revealing a molecular link between succinate dehydrogenase and purine synthesis, connecting two fundamental metabolic pathways. Huge congratulations to the first author, Mushtaq Nengroo! www.sciencedirect.com/science/arti...
!
!
Reposted by Ali Turk
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Ali Turk
"Our findings uncovered a species survival strategy in which telomerase RNA extends telomeres for the next generation by hitchhiking on introns of germline-expressed genes." 🤯
#Celegans 🧪
#Celegans 🧪
Nematode telomerase RNA hitchhikes on introns of germline–up-regulated genes
Telomerase is a ribonucleoprotein complex that elongates telomeric DNA, ensuring germline immortality. In this study, we identified the Caenorhabditis elegans telomerase RNA component 1 (terc-1), as t...
www.science.org
October 23, 2025 at 10:16 PM
"Our findings uncovered a species survival strategy in which telomerase RNA extends telomeres for the next generation by hitchhiking on introns of germline-expressed genes." 🤯
#Celegans 🧪
#Celegans 🧪
Reposted by Ali Turk
amazing #lipidtime story from Tontonoz lab! Lipid scrambling and cholesterol accessibility IN T-CELLS regulates nutrient uptake in small intestine 🤯🤯
Physiology is so weird!
www.science.org/doi/10.1126/...
Physiology is so weird!
www.science.org/doi/10.1126/...
T cell cholesterol transport links intestinal immune responses to dietary lipid absorption
The intrinsic pathways that control membrane organization in immune cells and their impact on cellular functions are poorly defined. We found that the nonvesicular cholesterol transporter Aster-A link...
www.science.org
October 17, 2025 at 6:33 PM
amazing #lipidtime story from Tontonoz lab! Lipid scrambling and cholesterol accessibility IN T-CELLS regulates nutrient uptake in small intestine 🤯🤯
Physiology is so weird!
www.science.org/doi/10.1126/...
Physiology is so weird!
www.science.org/doi/10.1126/...
Reposted by Ali Turk
Excited to share work out in @jacs.acspublications.org led by Yuxuan and @genlichem.bsky.social on activity-based sensing of acetaldehyde using an inverse electron-demand Diels-Alder reaction, enabling selective detection of two-carbon metabolism! pubs.acs.org/doi/10.1021/...
An Inverse Electron-Demand Diels–Alder Approach to Selective Activity-Based Sensing of Acetaldehyde in Living Cells
Acetaldehyde (AA) is a reactive aldehyde primarily produced in cells as a metabolic intermediate during ethanol oxidation. Excess AA, often resulting from impaired AA detoxification, leads to aberrant...
pubs.acs.org
October 16, 2025 at 7:22 PM
Excited to share work out in @jacs.acspublications.org led by Yuxuan and @genlichem.bsky.social on activity-based sensing of acetaldehyde using an inverse electron-demand Diels-Alder reaction, enabling selective detection of two-carbon metabolism! pubs.acs.org/doi/10.1021/...
Reposted by Ali Turk
You’ve heard of ubiquitination, meet deazaguanylation: Doug Wassarman in our lab discovered phage defense pathways have co-opted Q nucleobase biosynthetic enzymes to catalyze a new form of protein conjugation chemistry @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
September 25, 2025 at 7:16 PM
You’ve heard of ubiquitination, meet deazaguanylation: Doug Wassarman in our lab discovered phage defense pathways have co-opted Q nucleobase biosynthetic enzymes to catalyze a new form of protein conjugation chemistry @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Ali Turk
I don't have much intelligent things to say about MOFs other than "wow neat!" Also, there is an edible MOF paper: onlinelibrary.wiley.com/doi/abs/10.1...
#chemsky ⚗️🧪
#chemsky ⚗️🧪
Metal–Organic Frameworks from Edible Natural Products
Let them eat MOFs: Take a spoonful of sugar (γ-cyclodextrin to be precise), a pinch of salt (most alkali metal salts will suffice), and a swig of alcohol (Everclear fits the bill), and you have a rob...
onlinelibrary.wiley.com
October 9, 2025 at 4:44 PM
I don't have much intelligent things to say about MOFs other than "wow neat!" Also, there is an edible MOF paper: onlinelibrary.wiley.com/doi/abs/10.1...
#chemsky ⚗️🧪
#chemsky ⚗️🧪
Reposted by Ali Turk
KCTD10 is a sensor for co-directional transcription–replication conflicts @nature.com @mayoclinic.org
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 8, 2025 at 5:12 PM
KCTD10 is a sensor for co-directional transcription–replication conflicts @nature.com @mayoclinic.org
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ali Turk
Reposted by Ali Turk
How do cells sense & respond to lipid imbalances? What happens when a disease-relevant enzyme is blocked? Shiying Huang investigates phosphoinositide lipids with the Balla lab & discovers an integrated cellular response that boosts alternate lipid synthesis pathways! www.biorxiv.org/content/10.1...
October 3, 2025 at 1:03 PM
How do cells sense & respond to lipid imbalances? What happens when a disease-relevant enzyme is blocked? Shiying Huang investigates phosphoinositide lipids with the Balla lab & discovers an integrated cellular response that boosts alternate lipid synthesis pathways! www.biorxiv.org/content/10.1...
Reposted by Ali Turk
🔥After years of hard work, it's finally out in @natchem.nature.com🔥 The Hackenberger group @fmp-berlin.de developed #🎯DarT-labeling, a #photocatalyticproximitylabeling method using deazaflavin - a ✅ biocompatible ✅ easily synthesized ✅ chemically benign #photocatalyst
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Energy-transfer photoproximity labelling in live cells using an organic cofactor - Nature Chemistry
Current photoproximity labelling methods often require metal-based catalysts to map protein interactomes but, owing to their toxicity, they have limited intracellular applicability. A deazaflavin cofa...
www.nature.com
September 17, 2025 at 1:22 PM
🔥After years of hard work, it's finally out in @natchem.nature.com🔥 The Hackenberger group @fmp-berlin.de developed #🎯DarT-labeling, a #photocatalyticproximitylabeling method using deazaflavin - a ✅ biocompatible ✅ easily synthesized ✅ chemically benign #photocatalyst
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ali Turk
Beautiful reconstitution of amino acid stress-dependent ISR activation by my @harvardcellbio.bsky.social colleagues presenting a unifying mechanism for GCN2 activation, which requires ribosome collisions and is enhanced by cognate uncharged tRNA in the A site! www.science.org/doi/10.1126/...
GCN1 couples GCN2 to ribosomal state to initiate amino acid response pathway signaling
During nutrient deprivation, activation of the protein kinase GCN2 regulates cell survival and metabolic homeostasis. In addition to amino acid stress, GCN2 is activated by a variety of cellular stres...
www.science.org
October 3, 2025 at 11:02 AM
Beautiful reconstitution of amino acid stress-dependent ISR activation by my @harvardcellbio.bsky.social colleagues presenting a unifying mechanism for GCN2 activation, which requires ribosome collisions and is enhanced by cognate uncharged tRNA in the A site! www.science.org/doi/10.1126/...
Reposted by Ali Turk
Hypoxia-activated PROTACs (HAP-TACs) are online in @jacs.acspublications.org (pubs.acs.org/doi/10.1021/...)! Collborative work with Ester Hammond (@hammond-lab.bsky.social). Congrats to Marta Serafini, Sophie Twigger, George Delfas, Max Mallerman, Elliot Bailey & Ewen Calder (@ecalder.bsky.social).
Indolequinone-Based Hypoxia-Activated Proteolysis Targeting Chimeras Selectively Degrade BRD4 in Hypoxic Cancer Cells
Proteolysis targeting chimeras (PROTACs) have helped to establish proximity induction as an exciting strategy in drug discovery, and there are multiple clinical trials focused on this modality. Howeve...
pubs.acs.org
September 25, 2025 at 3:47 PM
Hypoxia-activated PROTACs (HAP-TACs) are online in @jacs.acspublications.org (pubs.acs.org/doi/10.1021/...)! Collborative work with Ester Hammond (@hammond-lab.bsky.social). Congrats to Marta Serafini, Sophie Twigger, George Delfas, Max Mallerman, Elliot Bailey & Ewen Calder (@ecalder.bsky.social).
Reposted by Ali Turk
