The Global Neurodegeneration Proteomics Consortium is a multi-national database that aims to leverage proteomics and clinical data to understand and improve ...

Siemens AG announced today that it has completed the acquisition of Dotmatics, a leading provider of Life Sciences R&D software headquartered in Boston and port

Deeper proteome coverage empowers new biological insights. Here, the authors establish a standard analysis workflow for TMT-based data acquired on the Orbitrap Astral mass spectrometer and leverage it...

Insights into how the linker histone H1 promotes chromatin alterations and allows repair of DNA damage are described.

Plasma proteomics may capture key insights into disease mechanisms and reveal informative biomarkers. Donoghue et al. apply a causal inference framework to genetic and plasma proteomic data from the U...

WHOOOOOOOOOOOOOOOOOOOOOOOOOOOOOAAAAAAAA!!!!! Dataset for reanalysis alert!!  Mann lab optimized perchlorate based enrichment to get a supe...

At-home DNA testing is no longer in high demand — and critics have raised concerns about unreliable tests and privacy.

The Byonic tutorial series covers MS/MS integration in Byos and Byosphere, optimizing peptide searches, interpreting search results, refining precursor charge, filtering PSMs, and generating peptide i...

Multistep multihour tryptic proteolysis has limited the utility of bottom-up proteomics for cases that require immediate quantitative information. The power of proteomics to quantify biomarkers of health status cannot practically assist in clinical care if the dynamics of disease outpaces the turnaround of analysis. The recently available hyperthermoacidic archaeal (HTA) protease “Krakatoa” digests samples in a single 5 to 30 min step at pH 3 and >80 °C in conditions that disrupt most cells and tissues, denature proteins, and block disulfide reformation thereby dramatically expediting and simplifying sample preparation. The combination of quick single-step proteolysis with high-throughput dual-trapping single analytical column (DTSC) liquid chromatography–mass spectrometry (LC–MS) returns actionable data in less than 1 h from collection of unprocessed biofluid. The systematic evaluation of this methodology finds that over 160 proteins are quantified in less than 1 h from 1 μL of whole blood. Furthermore, labile Angiotensin I and II bioactive peptides along with a panel of protein species can be measured at 8 min intervals with a 20 min initial lag using targeted MS. With these methods, we analyzed serum and plasma from 53 individuals and quantified Angiotensin I and II and over 150 proteins including at least 46 that were not detected with trypsin. We discuss some of the implications of real-time proteomics including the immediate potential to advance several clinical and research applications.

Nature - The device, to be tested in more people, could be used as a temporary measure for those waiting for a donor organ.

Nature Communications - While DIA has advanced standard proteomics, its application in glycoproteomics has remained limited. Here, the authors present an optimized narrow-window DIA method for...