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Reposted by Joachim Goedhart

Zebrafish Rock!

@zebrafishrock.bsky.social

🔗 to paper: www.sciencedirect.com/science/arti...

adamezracohen.bsky.social @adamezracohen.bsky.social · 18h

When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.

November 14, 2025 at 8:42 PM

Reposted by Joachim Goedhart

adamezracohen.bsky.social

@adamezracohen.bsky.social

When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.

November 14, 2025 at 8:34 PM

Reposted by Joachim Goedhart

Séamus Holden 🔬🦠🧫

@seamusholden.bsky.social

Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 1d

Alternatively, a DIY solution might be possible. If anyone has any ideas on a suitable non-toxic double-sided adhesive sheet material, then purchasing those and using either consumer grade die-cutter or online-die cutting services could be a straightforward solution.

November 14, 2025 at 10:44 AM

Reposted by Joachim Goedhart

Oded Rechavi

@odedrechavi.bsky.social

People in the audience looking at the y-axis labels

November 14, 2025 at 7:21 AM

Reposted by Joachim Goedhart

Patrick Lutz

@patricklutz.bsky.social

We didn't want to pay $800 for a TLC plate cutter, so we built one of our own – here's how you can too.

An Open-Source, Low-Cost Thin-Layer Chromatography Plate Cutter

Thin-layer chromatography (TLC) is widely used in teaching and research laboratories, but TLC plate cutters remain expensive, with most commercial models priced above $800 USD. This article presents a...

pubs.acs.org

November 13, 2025 at 8:18 PM

Reposted by Joachim Goedhart

Claire Deo

@clairedeo.bsky.social

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇

www.biorxiv.org/content/10.1...

November 13, 2025 at 4:20 PM

Joachim Goedhart

@joachimgoedhart.bsky.social

How genetically encoded sensors have lit up neuroscience
www.nature.com/articles/d41...

How genetically encoded sensors have lit up neuroscience

Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.

www.nature.com

November 13, 2025 at 3:17 PM

Reposted by Joachim Goedhart

adrinnenberg.bsky.social

@adrinnenberg.bsky.social

✨ New preprint ✨ What if we could watch AND control thousands of individual neurons in behaving mice—with stable access over months? 🧠⚡ Our work introduces transgenic all-optical tools that make this possible - and a resulting surprising discovery! 🔭💡
www.biorxiv.org/content/10.1... 1/13 🧵

Large-scale cellular-resolution read/write of activity enables discovery of cell types defined by complex circuit properties

The complexity of the mammalian brain’s vast population of interconnected neurons poses a formidable challenge to elucidate its underlying mechanisms of coordination and computation. A key step forwar...

www.biorxiv.org

November 13, 2025 at 12:24 AM

Reposted by Joachim Goedhart

Janice Tullock

@jtullock.bsky.social

No no no begs every archivist. You are never going to be able to find anything. Please don’t start using emojis in file names. Who asked for this? What fresh hell is next?

Screen grab from Dropbox saying Emojis in file names are finally here!

November 12, 2025 at 10:38 AM

Joachim Goedhart

@joachimgoedhart.bsky.social

Independent validation of the SnxA biosensor as a sensitive reporter of PI(3,5)P₂ dynamics by Gerald R Hammond and team: www.biorxiv.org/content/10.1...

(C) Recruitment of the WT PIKfyve-CCR-Kina to Rab5 endosomes and changes in biosensor localization at these membranes is
shown by the fluorescence intensity ratios of the constructs at Rab5 endosomes over the whole cell. Data shown is the grand
mean ± SEM of 3 experiments. A total of 35 cells were analyzed. Confocal images over time are shown to the right. (D) As in C,
but now utilizing the catalytically dead K1876E/V1883I mutant of the FKBP-PIKfyve-CCR-Kina as a negative control.

November 12, 2025 at 6:57 PM

Reposted by Joachim Goedhart

Juanma García-Arcos

@juanmagararc.bsky.social

Launching celldynamicslab.com, the homepage of our new group at EPFL working on cell fragmentation, membrane and cortex mechanics, FLIM imaging, and microfluidics tools. MSc/PhD or postdocs interested in quantitative cell biology are welcome to reach out. We're also hiring a lab manager in 2026!

Main - Cell Dynamics and Fragmentation Lab

The Cell Dynamics and Fragmentation lab at We study how single cells move and fragment in complex environments using microfluidics. We bring tools and concep...

celldynamicslab.com

November 11, 2025 at 5:10 PM

Reposted by Joachim Goedhart

Kevin Dean

@kevin-dean.bsky.social

A game changer in our hands... www.nature.com/articles/s41...

Universal consensus 3D segmentation of cells from 2D segmented stacks - Nature Methods

u-Segment3D is a universal framework that translates and enhances 2D instance segmentations to a 3D consensus instance segmentation without training data. It performs well across diverse datasets, inc...

www.nature.com

November 11, 2025 at 6:48 PM

Reposted by Joachim Goedhart

Mark A. Hanson

@hansonmark.bsky.social

For-profit journals are supposed to improve research quality, yet they're perversely incentivized to churn out whatever they can monetize. This was happening before AI (see Strain: bit.ly/43gJPUM), and AI will make it worse.

It's insane that we volunteer our time to help them do so.

4/n

November 11, 2025 at 11:52 AM

Reposted by Joachim Goedhart

Mark A. Hanson

@hansonmark.bsky.social

We wrote the Strain on scientific publishing to highlight the problems of time & trust. With a fantastic group of co-authors, we present The Drain of Scientific Publishing:

a 🧵 1/n

Drain: arxiv.org/abs/2511.04820
Strain: direct.mit.edu/qss/article/...
Oligopoly: direct.mit.edu/qss/article/...

A table showing profit margins of major publishers. A snippet of text related to this table is below.

1. The four-fold drain
1.1 Money
Currently, academic publishing is dominated by profit-oriented, multinational companies for
whom scientific knowledge is a commodity to be sold back to the academic community who
created it. The dominant four are Elsevier, Springer Nature, Wiley and Taylor & Francis,
which collectively generated over US$7.1 billion in revenue from journal publishing in 2024
alone, and over US$12 billion in profits between 2019 and 2024 (Table 1A). Their profit
margins have always been over 30% in the last five years, and for the largest publisher
(Elsevier) always over 37%.
Against many comparators, across many sectors, scientific publishing is one of the most
consistently profitable industries (Table S1). These financial arrangements make a substantial
difference to science budgets. In 2024, 46% of Elsevier revenues and 53% of Taylor &
Francis revenues were generated in North America, meaning that North American
researchers were charged over US$2.27 billion by just two for-profit publishers. The
Canadian research councils and the US National Science Foundation were allocated US$9.3
billion in that year.

A figure detailing the drain on researcher time.

1. The four-fold drain

1.2 Time
The number of papers published each year is growing faster than the scientific workforce,
with the number of papers per researcher almost doubling between 1996 and 2022 (Figure
1A). This reflects the fact that publishers’ commercial desire to publish (sell) more material
has aligned well with the competitive prestige culture in which publications help secure jobs,
grants, promotions, and awards. To the extent that this growth is driven by a pressure for
profit, rather than scholarly imperatives, it distorts the way researchers spend their time.
The publishing system depends on unpaid reviewer labour, estimated to be over 130 million
unpaid hours annually in 2020 alone (9). Researchers have complained about the demands of
peer-review for decades, but the scale of the problem is now worse, with editors reporting
widespread difficulties recruiting reviewers. The growth in publications involves not only the
authors’ time, but that of academic editors and reviewers who are dealing with so many
review demands.
Even more seriously, the imperative to produce ever more articles reshapes the nature of
scientific inquiry. Evidence across multiple fields shows that more papers result in
‘ossification’, not new ideas (10). It may seem paradoxical that more papers can slow
progress until one considers how it affects researchers’ time. While rewards remain tied to
volume, prestige, and impact of publications, researchers will be nudged away from riskier,
local, interdisciplinary, and long-term work. The result is a treadmill of constant activity with
limited progress whereas core scholarly practices – such as reading, reflecting and engaging
with others’ contributions – is de-prioritized. What looks like productivity often masks
intellectual exhaustion built on a demoralizing, narrowing scientific vision.

A table of profit margins across industries. The section of text related to this table is below:

1. The four-fold drain
1.1 Money
Currently, academic publishing is dominated by profit-oriented, multinational companies for
whom scientific knowledge is a commodity to be sold back to the academic community who
created it. The dominant four are Elsevier, Springer Nature, Wiley and Taylor & Francis,
which collectively generated over US$7.1 billion in revenue from journal publishing in 2024
alone, and over US$12 billion in profits between 2019 and 2024 (Table 1A). Their profit
margins have always been over 30% in the last five years, and for the largest publisher
(Elsevier) always over 37%.
Against many comparators, across many sectors, scientific publishing is one of the most
consistently profitable industries (Table S1). These financial arrangements make a substantial
difference to science budgets. In 2024, 46% of Elsevier revenues and 53% of Taylor &
Francis revenues were generated in North America, meaning that North American
researchers were charged over US$2.27 billion by just two for-profit publishers. The
Canadian research councils and the US National Science Foundation were allocated US$9.3
billion in that year.

The costs of inaction are plain: wasted public funds, lost researcher time, compromised
scientific integrity and eroded public trust. Today, the system rewards commercial publishers
first, and science second. Without bold action from the funders we risk continuing to pour
resources into a system that prioritizes profit over the advancement of scientific knowledge.

November 11, 2025 at 11:52 AM

Reposted by Joachim Goedhart

Vittorio Saggiomo

@vsaggiomo.bsky.social

Talking about impostor syndrome.....
I'll be a teacher in the EMBO "Hack your Microscope" together with some of the very best researchers in the field.
5.5 days of hacking and microscope/fluidics shenanigans in Portugal.
You can apply here: meetings.embo.org/event/26-mic... #EMBOmicroscope

Hack your microscope

Recent advances in open-source microscopy have made high-resolution, customizable imaging systems more accessible, allowing researchers to overcome the constraints of traditional commercial solutions…

meetings.embo.org

November 11, 2025 at 11:39 AM

Reposted by Joachim Goedhart

BioVoxxel (Jan Brocher)

@biovoxxel.de

Ever wondered if analyzing your images by using intensity Z-projections impacts your data?

Happy to announce my guest article in Microscopy & Analysis on that topic. It's a very short read but worth for everyone dealing with image analysis!

buff.ly/KJ147tI

#Microscopy #ZProjection #ImageAnalysis

November 10, 2025 at 4:05 PM

Reposted by Joachim Goedhart

Heather Brown-Harding

@microscopyed.bsky.social

What's some random autofluorescent stuff that I can find outside that would look cool on a microscope?

Looking for weird samples to highlight some of the instruments in the faciliity

November 10, 2025 at 3:15 PM

Reposted by Joachim Goedhart

Kevin K. Yang 楊凱筌

@kevinkaichuang.bsky.social

Design stable, folded proteins using only the 10 "ancient" amino acids.

www.biorxiv.org/content/10.1...

October 31, 2025 at 7:40 PM

Reposted by Joachim Goedhart

Joao Pereira

@jdpereira.bsky.social

(Before I drown myself discussing H1Bs on the bad place)

Does anyone know of a working promoter for tracking microglia?

a light that looks like a cat with a small face

ALT: a light that looks like a cat with a small face

media.tenor.com

November 8, 2025 at 9:39 PM

Reposted by Joachim Goedhart

Andrew Heiss

@andrew.heiss.phd

Have you ever wanted to make your own climate warming stripes plot? It's super easy with #rstats! (code here gist.github.com/andrewheiss/... )

Blue and red stripes showing temperature anomalies since 1850

November 7, 2025 at 10:14 PM

Reposted by Joachim Goedhart

Pablo J. Sáez

@pjsaez.bsky.social

I love to see the endoplasmic reticulum (ER, cyan) hitchhiking over microtubules (MTs, orange).

Also the membrane dynamics are lovely.

It's been a while, but here we are for a late #fluorescencefriday with some #microscopy
#scicomm #scisky @cellcommlab.bsky.social focalplane.bsky.social 🧪🔬