Dmitry Kretov
@dmitry-kretov.bsky.social
Group Leader @Centre de Recherche du CHU de Québec-Université Laval #NewPI
RNA-binding proteins and microRNAs in development and beyond.
RNA-binding proteins and microRNAs in development and beyond.
Reposted by Dmitry Kretov
new Lai lab paper @natsmb.nature.com! Dicer is specifically mutated in cancer, but we don't fully understand its molecular/reg impacts. with @danweihuangfu.bsky.social, we characterized the first knockin Dicer hotspot in hESCs, and found unexpected defects in miRNA biogenesis! 🧬 1/4
rdcu.be/eOc0q
rdcu.be/eOc0q
Human DICER1 hotspot mutation induces both loss and gain of miRNA function
Nature Structural & Molecular Biology - Jee et al. study a cancer hotspot allele of DICER1 that disrupts RNaseIIIb activity. Beyond ablating 5p hairpin cleavage, 3p passenger strands are...
rdcu.be
November 10, 2025 at 4:43 PM
new Lai lab paper @natsmb.nature.com! Dicer is specifically mutated in cancer, but we don't fully understand its molecular/reg impacts. with @danweihuangfu.bsky.social, we characterized the first knockin Dicer hotspot in hESCs, and found unexpected defects in miRNA biogenesis! 🧬 1/4
rdcu.be/eOc0q
rdcu.be/eOc0q
Reposted by Dmitry Kretov
Gentle reminder that everyone should read Jacob and Monod, 1961. And then read it again every once in awhile. A master-class on clear writing (and, ya know, Nobel Prize winning stuff, too).
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Genetic regulatory mechanisms in the synthesis of proteins
The synthesis of enzymes in bacteria follows a double genetic control. The socalled structural genes determine the molecular organization of the prote…
www.sciencedirect.com
November 10, 2025 at 3:28 PM
Gentle reminder that everyone should read Jacob and Monod, 1961. And then read it again every once in awhile. A master-class on clear writing (and, ya know, Nobel Prize winning stuff, too).
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Dmitry Kretov
New paper alert! Scientists in Clemens Plaschka’s lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
November 6, 2025 at 4:04 PM
New paper alert! Scientists in Clemens Plaschka’s lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
Reposted by Dmitry Kretov
📅 Save the Date!
The 4th Canadian Zebrafish Research Conference will take place on Friday, May 15, 2026, at CHU Sainte-Justine, Montreal (just before the Canadian Neuroscience Conference!)
Multiple travel awards available with 2 Postdoctoral Rising Stars Awards! #izfs
The 4th Canadian Zebrafish Research Conference will take place on Friday, May 15, 2026, at CHU Sainte-Justine, Montreal (just before the Canadian Neuroscience Conference!)
Multiple travel awards available with 2 Postdoctoral Rising Stars Awards! #izfs
November 6, 2025 at 3:31 PM
📅 Save the Date!
The 4th Canadian Zebrafish Research Conference will take place on Friday, May 15, 2026, at CHU Sainte-Justine, Montreal (just before the Canadian Neuroscience Conference!)
Multiple travel awards available with 2 Postdoctoral Rising Stars Awards! #izfs
The 4th Canadian Zebrafish Research Conference will take place on Friday, May 15, 2026, at CHU Sainte-Justine, Montreal (just before the Canadian Neuroscience Conference!)
Multiple travel awards available with 2 Postdoctoral Rising Stars Awards! #izfs
Reposted by Dmitry Kretov
Now online! Disparate leukemia mutations converge on nuclear phase-separated condensates
Disparate leukemia mutations converge on nuclear phase-separated condensates
Mutant NPM1 and various leukemia oncofusions form biophysically indistingishable nuclear condensates, termed C-bodies, which orchestrate leukemogenic gene expression. These findings consolidate diverse genetic lesions into a shared pathogenic mechanism in AML.
dlvr.it
November 4, 2025 at 7:51 PM
Now online! Disparate leukemia mutations converge on nuclear phase-separated condensates
Reposted by Dmitry Kretov
This is such an interesting paper. Why? Because the binding of transcription factor (TF) proteins to DNA governs how our genes are turned on/off/up/down, & so is the primary issue for how our genes work in development and how our cells respond to just about anything.🧵
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Multiple overlapping binding sites determine transcription factor occupancy - Nature
A new method enables comprehensive screening and identification of low-affinity DNA binding sites for transcription factors, and reveals that nucleotides flanking high-affinity binding sites create ov...
www.nature.com
October 30, 2025 at 11:04 PM
This is such an interesting paper. Why? Because the binding of transcription factor (TF) proteins to DNA governs how our genes are turned on/off/up/down, & so is the primary issue for how our genes work in development and how our cells respond to just about anything.🧵
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Dmitry Kretov
Colliding ribosomes are potent signals of cellular stress. But do cells use ‘programmed’ ribosome collisions to regulate gene expression? I’m excited to present a new story from my lab led by Frederick Rehfeld(@fred-rehfeld.bsky.social) which revealed that the answer is YES! Read on to find out how👇
Oxidative stress sensing by the translation elongation machinery promotes production of detoxifying selenoproteins
Selenocysteine, incorporated into polypeptides at recoded termination codons, plays an essential role in redox biology. Using GPX1 and GPX4, selenoenzymes that mitigate oxidative stress, as reporters,...
www.biorxiv.org
October 14, 2025 at 10:28 PM
Colliding ribosomes are potent signals of cellular stress. But do cells use ‘programmed’ ribosome collisions to regulate gene expression? I’m excited to present a new story from my lab led by Frederick Rehfeld(@fred-rehfeld.bsky.social) which revealed that the answer is YES! Read on to find out how👇
Reposted by Dmitry Kretov
Check out the latest work from our lab, led by Arash Latifkar @ara-latifkar.bsky.social , www.biorxiv.org/content/10.1...
mRNA poly(A)-tail length is a battleground for coronavirus–host competition
Most eukaryotic mRNAs contain a poly(A) tail, which in post-embryonic cells enhances their stability. Many cytoplasmic RNA viruses also harbor poly(A) tails on their genomic RNA and mRNAs. Here, we re...
www.biorxiv.org
October 13, 2025 at 7:44 PM
Check out the latest work from our lab, led by Arash Latifkar @ara-latifkar.bsky.social , www.biorxiv.org/content/10.1...
Reposted by Dmitry Kretov
(1/4) New Pre-print! 🌟
Embryonic development is usually seen as a continuous process, yet some embryos can pause their development to survive harsh conditions, a phenomenon known as diapause. How this pausing is actively maintained has remained a mystery.
Embryonic development is usually seen as a continuous process, yet some embryos can pause their development to survive harsh conditions, a phenomenon known as diapause. How this pausing is actively maintained has remained a mystery.
October 9, 2025 at 8:08 PM
(1/4) New Pre-print! 🌟
Embryonic development is usually seen as a continuous process, yet some embryos can pause their development to survive harsh conditions, a phenomenon known as diapause. How this pausing is actively maintained has remained a mystery.
Embryonic development is usually seen as a continuous process, yet some embryos can pause their development to survive harsh conditions, a phenomenon known as diapause. How this pausing is actively maintained has remained a mystery.
Reposted by Dmitry Kretov
Really grateful to see our work featured by @quantamagazine.bsky.social in this piece on the evolution of genome regulation. Huge thanks to @philipcball.bsky.social for such a beautifully written article.
I adored writing this piece. It brings together several of the things preoccupying me right now, like chromatin organization and gene regulation. There's so much more to be said on that. Also, these marine critters look gorgeous.
www.quantamagazine.org/loops-of-dna...
www.quantamagazine.org/loops-of-dna...
Loops of DNA Equipped Ancient Life To Become Complex | Quanta Magazine
New work shows that physical folding of the genome to control genes located far away may have been an early evolutionary development.
www.quantamagazine.org
October 8, 2025 at 8:49 PM
Really grateful to see our work featured by @quantamagazine.bsky.social in this piece on the evolution of genome regulation. Huge thanks to @philipcball.bsky.social for such a beautifully written article.
Reposted by Dmitry Kretov
Reposted by Dmitry Kretov
#ZebrafishFunFacts: As the most-cited #zebrafish cancer research paper, Amsterdam et al screened hundreds of heterozygous lines for embryonic lethal mutations & found elevated cancer incidence in ribosomal genes. This previously underappreciated finding was published @plosbiology.org 21 years ago. 🧪
October 4, 2025 at 7:23 AM
#ZebrafishFunFacts: As the most-cited #zebrafish cancer research paper, Amsterdam et al screened hundreds of heterozygous lines for embryonic lethal mutations & found elevated cancer incidence in ribosomal genes. This previously underappreciated finding was published @plosbiology.org 21 years ago. 🧪
Reposted by Dmitry Kretov
We combine our yeast genetics experiments with the massive body of literature data to make a case for 40S scanning of 5’UTRs of by 1D diffusion (finalised version): m.rnajournal.cshlp.org/content/31/1...
RNA | Mobile
https://m.rnajournal.cshlp.org/content/31/10/…
October 4, 2025 at 2:05 AM
We combine our yeast genetics experiments with the massive body of literature data to make a case for 40S scanning of 5’UTRs of by 1D diffusion (finalised version): m.rnajournal.cshlp.org/content/31/1...
Reposted by Dmitry Kretov
1/5 Lab's first manuscript 🎉 is out @biorxivpreprint.bsky.social today! Beautifully, it is performed by a senior undergraduate thesis student, Mohammed! We developed a 3-D printed #zebrafish embryo imaging chamber 🔬and connected it to syringe pumps to be able to perfuse drugs in a timely fashion. 🧪
October 2, 2025 at 2:27 AM
1/5 Lab's first manuscript 🎉 is out @biorxivpreprint.bsky.social today! Beautifully, it is performed by a senior undergraduate thesis student, Mohammed! We developed a 3-D printed #zebrafish embryo imaging chamber 🔬and connected it to syringe pumps to be able to perfuse drugs in a timely fashion. 🧪
Reposted by Dmitry Kretov
RNA-binding proteins provide specificity to the PAN2-PAN3 mRNA deadenylation complex https://www.biorxiv.org/content/10.1101/2025.09.27.678968v1
September 27, 2025 at 8:17 PM
RNA-binding proteins provide specificity to the PAN2-PAN3 mRNA deadenylation complex https://www.biorxiv.org/content/10.1101/2025.09.27.678968v1
Congratulations Markus! Really cool work!👏
www.biorxiv.org/content/10.1...
Delighted to share the story of two germline RBPs - one with little (DND1) and one with no (NANOS3) intrinsic sequence-specificity - that together build a continuous RNA binding surface recognizing a 7-mer (AUGAAUU) in target mRNA 3’UTRs, leading to deadenylation.
Delighted to share the story of two germline RBPs - one with little (DND1) and one with no (NANOS3) intrinsic sequence-specificity - that together build a continuous RNA binding surface recognizing a 7-mer (AUGAAUU) in target mRNA 3’UTRs, leading to deadenylation.
The DND1-NANOS3 complex shapes the primordial germ cell transcriptome via a heptanucleotide sequence in mRNA 3'UTRs
The RNA-binding proteins DND1 and NANOS3 are essential for primordial germ cell survival. Their co-immunoprecipitation and overlapping loss-of-function phenotypes suggest joint function, yet how they ...
www.biorxiv.org
September 27, 2025 at 3:56 PM
Congratulations Markus! Really cool work!👏
Reposted by Dmitry Kretov
This is a fascinating paper that particular types of RNA binding proteins with IDRs that target nuclear speckles also can recruit their own RNAs to nuclear speckles as a negative feedback mechanism for condensation the authors call "interstasis" www.nature.com/articles/s41...
Collective homeostasis of condensation-prone proteins via their mRNAs - Nature
The authors discover a homeostatic process termed interstasis, in which an increased concentration of proteins within RNA–protein condensates induces the sequestration of their own mRNAs.
www.nature.com
September 25, 2025 at 8:24 PM
This is a fascinating paper that particular types of RNA binding proteins with IDRs that target nuclear speckles also can recruit their own RNAs to nuclear speckles as a negative feedback mechanism for condensation the authors call "interstasis" www.nature.com/articles/s41...
Reposted by Dmitry Kretov
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Dmitry Kretov
We quantified mRNA abundance, translation, protein abundance, protein degradation and cell growth across thousands of single cells from a mammalian tissue.
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
September 21, 2025 at 11:07 AM
We quantified mRNA abundance, translation, protein abundance, protein degradation and cell growth across thousands of single cells from a mammalian tissue.
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
Reposted by Dmitry Kretov
Reposted by Dmitry Kretov
It is my pleasure to share with you the latest from @jsvejstrup.bsky.social lab, where we look at how the reduction of RNAPII levels has a severe, yet organized transcriptional response in the cell.
September 19, 2025 at 9:15 AM
It is my pleasure to share with you the latest from @jsvejstrup.bsky.social lab, where we look at how the reduction of RNAPII levels has a severe, yet organized transcriptional response in the cell.
Congrats, Eric and Renfu!!!
new Lai lab #miRNA paper alert!! happy to share Renfu Shang's mechanistic insight into mysteries of microRNA cluster assistance. wonderful collab with Niko Popitsch and @ameressl.bsky.social.
🧵 later, but if you don't know this process, my daughter illustrated it. 🧬
www.biorxiv.org/content/10.1...
🧵 later, but if you don't know this process, my daughter illustrated it. 🧬
www.biorxiv.org/content/10.1...
September 20, 2025 at 12:00 AM
Congrats, Eric and Renfu!!!
Reposted by Dmitry Kretov
Dream collaboration with the groups of Torben Heick Jensen (@heick.bsky.social; @au.dk) and Clemens Plaschka (@impvienna.bsky.social). Building in part on the incredible work from Ulrich Hohmann on human mRNA export.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A molecular switch orchestrates the nuclear export of human messenger RNA
The nuclear export of messenger RNA (mRNA) is a key step in eukaryotic gene expression ([Köhler and Hurt, 2007][1]). Despite recent insights into the packaging of newly transcribed mRNAs into ribonucl...
www.biorxiv.org
September 17, 2025 at 12:42 PM
Dream collaboration with the groups of Torben Heick Jensen (@heick.bsky.social; @au.dk) and Clemens Plaschka (@impvienna.bsky.social). Building in part on the incredible work from Ulrich Hohmann on human mRNA export.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Dmitry Kretov
How do cells sort which RNAs to keep or destroy? New preprint from THJ, Brenneke and Plaschka labs shows that export and decay machineries (TREX2/PAXT) both recognise UAP56-bound RNAs. Whether they’re exported or degraded depends on where in the nucleus this happens.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Molecular basis of polyadenylated RNA fate determination in the nucleus
Eukaryotic genomes generate a plethora of polyadenylated (pA+) RNAs, that are packaged into ribonucleoprotein particles (RNPs). To ensure faithful gene expression, functional pA+ RNPs, including prote...
www.biorxiv.org
September 17, 2025 at 10:31 AM
How do cells sort which RNAs to keep or destroy? New preprint from THJ, Brenneke and Plaschka labs shows that export and decay machineries (TREX2/PAXT) both recognise UAP56-bound RNAs. Whether they’re exported or degraded depends on where in the nucleus this happens.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Dmitry Kretov
Excited to share our recent work about the role of the molecular chaperone TRAP1 as RNA-binding protein! #RBPs #HGSOC
TRAP1 moonlights as an RNA-binding protein, enhancing translation of the splicing factor LUC7L3 to drive proliferation in high-grade serous ovarian cancer cells. #TRAP1 #RBP #ovariancancer bit.ly/47t3apu
September 13, 2025 at 11:15 AM