Slavov Laboratory
@slavovlab.bsky.social
We seek principles in the coordination among protein synthesis, metabolism, cell growth and differentiation. PI: @slavov-n.bsky.social
Web: https://slavovlab.net
Videos: http://youtube.slavovlab.net
Web: https://slavovlab.net
Videos: http://youtube.slavovlab.net
Discussing research with the innovators beats scrolling a feed.
If you’re in single-cell biology, proteomics, mass spec tech, or computational biology, join our next meeting this July.
#SCP2026
If you’re in single-cell biology, proteomics, mass spec tech, or computational biology, join our next meeting this July.
#SCP2026
Single-cell proteomics is moving past descriptive snapshots to quantitative models of cellular regulation and biological mechanisms.
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
January 20, 2026 at 1:53 PM
Discussing research with the innovators beats scrolling a feed.
If you’re in single-cell biology, proteomics, mass spec tech, or computational biology, join our next meeting this July.
#SCP2026
If you’re in single-cell biology, proteomics, mass spec tech, or computational biology, join our next meeting this July.
#SCP2026
Reposted by Slavov Laboratory
Single-cell proteomics is moving past descriptive snapshots to quantitative models of cellular regulation and biological mechanisms.
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
January 20, 2026 at 1:48 PM
Single-cell proteomics is moving past descriptive snapshots to quantitative models of cellular regulation and biological mechanisms.
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
Protein degradation is often considered a final step in gene expression regulation, but it also regulates the first step: Transcription
Many TFs, including p53, HIF-1α and c-Myc, are regulated by 𝐝𝐞𝐠𝐫𝐚𝐝𝐚𝐭𝐢𝐨𝐧-𝐠𝐚𝐭𝐞𝐝 𝐜𝐨𝐧𝐭𝐫𝐨𝐥:
⬛️ It has a key advantage.
blog.slavovlab.net/2025/08/16/p...
Many TFs, including p53, HIF-1α and c-Myc, are regulated by 𝐝𝐞𝐠𝐫𝐚𝐝𝐚𝐭𝐢𝐨𝐧-𝐠𝐚𝐭𝐞𝐝 𝐜𝐨𝐧𝐭𝐫𝐨𝐥:
⬛️ It has a key advantage.
blog.slavovlab.net/2025/08/16/p...
Proteins made to die
Protein synthesis is among the most energy-demanding processes in a cell. Yet, some proteins are synthesized and immediately degraded. This may seem like a futile cycle, but it’s a remarkable…
blog.slavovlab.net
January 19, 2026 at 12:32 PM
Protein degradation is often considered a final step in gene expression regulation, but it also regulates the first step: Transcription
Many TFs, including p53, HIF-1α and c-Myc, are regulated by 𝐝𝐞𝐠𝐫𝐚𝐝𝐚𝐭𝐢𝐨𝐧-𝐠𝐚𝐭𝐞𝐝 𝐜𝐨𝐧𝐭𝐫𝐨𝐥:
⬛️ It has a key advantage.
blog.slavovlab.net/2025/08/16/p...
Many TFs, including p53, HIF-1α and c-Myc, are regulated by 𝐝𝐞𝐠𝐫𝐚𝐝𝐚𝐭𝐢𝐨𝐧-𝐠𝐚𝐭𝐞𝐝 𝐜𝐨𝐧𝐭𝐫𝐨𝐥:
⬛️ It has a key advantage.
blog.slavovlab.net/2025/08/16/p...
Transformative discoveries, such as restriction enzymes, yeast cell-cycle mutants and CRISPR–Cas9, were once considered simply oddities of nature.
Let’s adopt more humility about predicting impact, which can truly be known only in retrospect.
Let’s adopt more humility about predicting impact, which can truly be known only in retrospect.
This view resonates deeply with me:
⬛ Publish fewer claims and more proof
Papers need to include fewer claims and more proof to make the scientific literature more reliable
What is driving today’s ‘claims inflation’?
1/3
⬛ Publish fewer claims and more proof
Papers need to include fewer claims and more proof to make the scientific literature more reliable
What is driving today’s ‘claims inflation’?
1/3
January 9, 2026 at 1:36 PM
Transformative discoveries, such as restriction enzymes, yeast cell-cycle mutants and CRISPR–Cas9, were once considered simply oddities of nature.
Let’s adopt more humility about predicting impact, which can truly be known only in retrospect.
Let’s adopt more humility about predicting impact, which can truly be known only in retrospect.
Happy and successful 2026 !
It will be a productive one for single-cell proteomics.
single-cell.net/proteomics/s...
It will be a productive one for single-cell proteomics.
single-cell.net/proteomics/s...
January 6, 2026 at 1:05 PM
Happy and successful 2026 !
It will be a productive one for single-cell proteomics.
single-cell.net/proteomics/s...
It will be a productive one for single-cell proteomics.
single-cell.net/proteomics/s...
Our latest article is out in Cell !
Together with the Zernicka-Goetz lab, we show that fertilization triggers proteomic asymmetry in mammalian zygotes, giving rise to alpha vs. beta 2-cell blastomeres with distinct developmental potential.
cell.com/cell/fulltex...
Together with the Zernicka-Goetz lab, we show that fertilization triggers proteomic asymmetry in mammalian zygotes, giving rise to alpha vs. beta 2-cell blastomeres with distinct developmental potential.
cell.com/cell/fulltex...
December 3, 2025 at 4:41 PM
Our latest article is out in Cell !
Together with the Zernicka-Goetz lab, we show that fertilization triggers proteomic asymmetry in mammalian zygotes, giving rise to alpha vs. beta 2-cell blastomeres with distinct developmental potential.
cell.com/cell/fulltex...
Together with the Zernicka-Goetz lab, we show that fertilization triggers proteomic asymmetry in mammalian zygotes, giving rise to alpha vs. beta 2-cell blastomeres with distinct developmental potential.
cell.com/cell/fulltex...
Reposted by Slavov Laboratory
The interview covers the trajectory from early, high-risk ideas to robust, scalable workflows.
Much has changed, and progress continues unabated.
Much has changed, and progress continues unabated.
This feature highlights early advances from our group in liquid-chromatography mass spectrometry (LC-MS) for single-cell proteomics.
center.single-cell.net/single-cell-...
center.single-cell.net/single-cell-...
November 29, 2025 at 1:55 PM
The interview covers the trajectory from early, high-risk ideas to robust, scalable workflows.
Much has changed, and progress continues unabated.
Much has changed, and progress continues unabated.
Reposted by Slavov Laboratory
𝗔𝗯𝘀𝘁𝗿𝗮𝗰𝘁 𝘀𝘂𝗯𝗺𝗶𝘀𝘀𝗶𝗼𝗻 𝗶𝘀 𝗼𝗽𝗲𝗻 !
The 9th Single Cell Proteomics Conference (#SCP2026) will take place in #Boston, July 2026.
The Single Cell Proteomics Conference focuses on providing opportunities for scientific discussions, interactions and community development.
single-cell.net/proteomics/s...
The 9th Single Cell Proteomics Conference (#SCP2026) will take place in #Boston, July 2026.
The Single Cell Proteomics Conference focuses on providing opportunities for scientific discussions, interactions and community development.
single-cell.net/proteomics/s...
December 1, 2025 at 11:54 AM
𝗔𝗯𝘀𝘁𝗿𝗮𝗰𝘁 𝘀𝘂𝗯𝗺𝗶𝘀𝘀𝗶𝗼𝗻 𝗶𝘀 𝗼𝗽𝗲𝗻 !
The 9th Single Cell Proteomics Conference (#SCP2026) will take place in #Boston, July 2026.
The Single Cell Proteomics Conference focuses on providing opportunities for scientific discussions, interactions and community development.
single-cell.net/proteomics/s...
The 9th Single Cell Proteomics Conference (#SCP2026) will take place in #Boston, July 2026.
The Single Cell Proteomics Conference focuses on providing opportunities for scientific discussions, interactions and community development.
single-cell.net/proteomics/s...
This feature highlights early advances from our group in liquid-chromatography mass spectrometry (LC-MS) for single-cell proteomics.
center.single-cell.net/single-cell-...
center.single-cell.net/single-cell-...
November 29, 2025 at 1:16 PM
This feature highlights early advances from our group in liquid-chromatography mass spectrometry (LC-MS) for single-cell proteomics.
center.single-cell.net/single-cell-...
center.single-cell.net/single-cell-...
Mass spectrometry enables omics scale quantification of the rates of macromolecule synthesis and degradation in the single cells of a living mammalian tissue.
1/3
1/3
Single-cell omics methods are mighty but bound to static snapshot when analyzing 𝑖𝑛 𝑣𝑖𝑣𝑜 tissues.
Mass spec offers a solution.
⬛️ It enables omics scale quantification of the rates of macromolecule synthesis and degradation in a living organism.
blog.slavovlab.net/2025/11/19/i...
Mass spec offers a solution.
⬛️ It enables omics scale quantification of the rates of macromolecule synthesis and degradation in a living organism.
blog.slavovlab.net/2025/11/19/i...
November 20, 2025 at 2:59 PM
Mass spectrometry enables omics scale quantification of the rates of macromolecule synthesis and degradation in the single cells of a living mammalian tissue.
1/3
1/3
The majority of isoform pairs (of alternatively spliced transcripts) share less than 50% of their interactions.
In the context of interactome networks, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other.
In the context of interactome networks, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other.
Proteoforms encoded by the same gene have different interactomes.
This challenges:
1⃣ The interpretation of data from affinity reagents directed towards shared epitopes.
2⃣ The assignment of functions to genes.
When will biological research focus on such functional differences?
This challenges:
1⃣ The interpretation of data from affinity reagents directed towards shared epitopes.
2⃣ The assignment of functions to genes.
When will biological research focus on such functional differences?
November 6, 2025 at 1:41 PM
The majority of isoform pairs (of alternatively spliced transcripts) share less than 50% of their interactions.
In the context of interactome networks, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other.
In the context of interactome networks, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other.
Protein degradation plays major roles in aging physiology & pathology.
We can start analyzing it quantitatively at higher resolution & scale.
A promising new direction for aging research 🚀
We can start analyzing it quantitatively at higher resolution & scale.
A promising new direction for aging research 🚀
Aging is associated with changes in protein abundance in many organs and tissues.
We find that the protein changes are cell-type specific, and thus muted in previous bulk analysis.
Protein clearance rates change with age and drive the ...
www.biorxiv.org/content/10.1...
We find that the protein changes are cell-type specific, and thus muted in previous bulk analysis.
Protein clearance rates change with age and drive the ...
www.biorxiv.org/content/10.1...
October 30, 2025 at 2:52 PM
Protein degradation plays major roles in aging physiology & pathology.
We can start analyzing it quantitatively at higher resolution & scale.
A promising new direction for aging research 🚀
We can start analyzing it quantitatively at higher resolution & scale.
A promising new direction for aging research 🚀
Mechanotransduction in the nucleus
Multiple TFs transmit mechanical forces into transcriptional regulation.
Nuclear shape, size, and chromatin abnormalities are common features of many diseases.
Multiple TFs transmit mechanical forces into transcriptional regulation.
Nuclear shape, size, and chromatin abnormalities are common features of many diseases.
October 27, 2025 at 9:48 AM
Mechanotransduction in the nucleus
Multiple TFs transmit mechanical forces into transcriptional regulation.
Nuclear shape, size, and chromatin abnormalities are common features of many diseases.
Multiple TFs transmit mechanical forces into transcriptional regulation.
Nuclear shape, size, and chromatin abnormalities are common features of many diseases.
October 25, 2025 at 12:38 PM
Reposted by Slavov Laboratory
The big picture:
Tracing the arc of conceptual milestones in life sciences over the past century, centered around major discoveries driven by evolving technologies.
We are excited about the progress & the research enabled by tech from @ParallelSqTech.
youtu.be/SrzoLGBVTsY?...
Tracing the arc of conceptual milestones in life sciences over the past century, centered around major discoveries driven by evolving technologies.
We are excited about the progress & the research enabled by tech from @ParallelSqTech.
youtu.be/SrzoLGBVTsY?...
Proteomics: The arc of progress in life sciences | Research Fest by PTI
YouTube video by Nikolai Slavov
youtu.be
October 24, 2025 at 7:38 PM
The big picture:
Tracing the arc of conceptual milestones in life sciences over the past century, centered around major discoveries driven by evolving technologies.
We are excited about the progress & the research enabled by tech from @ParallelSqTech.
youtu.be/SrzoLGBVTsY?...
Tracing the arc of conceptual milestones in life sciences over the past century, centered around major discoveries driven by evolving technologies.
We are excited about the progress & the research enabled by tech from @ParallelSqTech.
youtu.be/SrzoLGBVTsY?...
A highlight of our results on alternate RNA decoding.
When the weirdo variant protein versions outnumber the “real” ones:
When Variant Proteins Aren't Actually the Variant Ones
www.science.org
October 25, 2025 at 12:30 PM
A highlight of our results on alternate RNA decoding.
Reposted by Slavov Laboratory
This article is a homecoming for me.
As a PhD student, I focused on the growth-rate transcriptional regulation in yeast.
Now, ~ 20 years later, we report protein regulation scaling with the growth rates of single cells in mammalian tissues.
www.biorxiv.org/content/10.1...
As a PhD student, I focused on the growth-rate transcriptional regulation in yeast.
Now, ~ 20 years later, we report protein regulation scaling with the growth rates of single cells in mammalian tissues.
www.biorxiv.org/content/10.1...
Principles of protein abundance regulation across single cells in a mammalian tissue
Protein synthesis and clearance are major regulatory steps of gene expression, but their in vivo regulatory roles across the cells comprising complex tissues remains unexplored. Here, we systematicall...
www.biorxiv.org
October 18, 2025 at 11:56 AM
This article is a homecoming for me.
As a PhD student, I focused on the growth-rate transcriptional regulation in yeast.
Now, ~ 20 years later, we report protein regulation scaling with the growth rates of single cells in mammalian tissues.
www.biorxiv.org/content/10.1...
As a PhD student, I focused on the growth-rate transcriptional regulation in yeast.
Now, ~ 20 years later, we report protein regulation scaling with the growth rates of single cells in mammalian tissues.
www.biorxiv.org/content/10.1...
Is your research important ?
If important, make it count.
Make it easy to reproduce.
Share the data.
Share the code.
Share the metadata.
Use community guidelines to facilitate reanalysis & reuse of your results.
nature.com/articles/s41...
If important, make it count.
Make it easy to reproduce.
Share the data.
Share the code.
Share the metadata.
Use community guidelines to facilitate reanalysis & reuse of your results.
nature.com/articles/s41...
October 18, 2025 at 11:53 AM
Is your research important ?
If important, make it count.
Make it easy to reproduce.
Share the data.
Share the code.
Share the metadata.
Use community guidelines to facilitate reanalysis & reuse of your results.
nature.com/articles/s41...
If important, make it count.
Make it easy to reproduce.
Share the data.
Share the code.
Share the metadata.
Use community guidelines to facilitate reanalysis & reuse of your results.
nature.com/articles/s41...
What is the relationship between the transcriptional induction of a gene and the fitness effect of its deletion ?
This study reported remarkably low correlations in the context of heat shock response and survival.
This study reported remarkably low correlations in the context of heat shock response and survival.
October 13, 2025 at 1:31 PM
What is the relationship between the transcriptional induction of a gene and the fitness effect of its deletion ?
This study reported remarkably low correlations in the context of heat shock response and survival.
This study reported remarkably low correlations in the context of heat shock response and survival.
Reposted by Slavov Laboratory
An amazing feat of regulation.
Two very different regulatory mechanisms converge to shape the mitochondrial proteome and keep us alive!
Two very different regulatory mechanisms converge to shape the mitochondrial proteome and keep us alive!
September 27, 2025 at 10:50 AM
An amazing feat of regulation.
Two very different regulatory mechanisms converge to shape the mitochondrial proteome and keep us alive!
Two very different regulatory mechanisms converge to shape the mitochondrial proteome and keep us alive!
Reposted by Slavov Laboratory
mRNA-mRNA correlations across the single cells from a tissue are rarely interpreted.
They differ from the corresponding protein-protein correlations?
𝐖𝐡𝐲 ❓
www.biorxiv.org/content/10.1...
🧵
They differ from the corresponding protein-protein correlations?
𝐖𝐡𝐲 ❓
www.biorxiv.org/content/10.1...
🧵
September 29, 2025 at 10:46 AM
mRNA-mRNA correlations across the single cells from a tissue are rarely interpreted.
They differ from the corresponding protein-protein correlations?
𝐖𝐡𝐲 ❓
www.biorxiv.org/content/10.1...
🧵
They differ from the corresponding protein-protein correlations?
𝐖𝐡𝐲 ❓
www.biorxiv.org/content/10.1...
🧵
The regulation of nuclear-encoded mitochondrial proteins is fascinating.
They are synthesized in a fully eukaryotic manner, with introns, splicing, and cytosolic ribosomes.
Yet, they are degraded by proteases that look and act bacterial.
blog.slavovlab.net/2025/09/23/m...
They are synthesized in a fully eukaryotic manner, with introns, splicing, and cytosolic ribosomes.
Yet, they are degraded by proteases that look and act bacterial.
blog.slavovlab.net/2025/09/23/m...
Mixing eukaryotic synthesis with prokaryotic degradation
The Evolutionary Balancing Act Coordinating gene regulation across two fundamentally different systems is one of the great balancing acts of life. Mitochondria embody this challenge: most of their …
blog.slavovlab.net
September 24, 2025 at 12:10 AM
The regulation of nuclear-encoded mitochondrial proteins is fascinating.
They are synthesized in a fully eukaryotic manner, with introns, splicing, and cytosolic ribosomes.
Yet, they are degraded by proteases that look and act bacterial.
blog.slavovlab.net/2025/09/23/m...
They are synthesized in a fully eukaryotic manner, with introns, splicing, and cytosolic ribosomes.
Yet, they are degraded by proteases that look and act bacterial.
blog.slavovlab.net/2025/09/23/m...
Reposted by Slavov Laboratory
Some proteins are primarily regulated by one mechanism: RNA abundance, translation, or clearance.
The regulation of most proteins is dominated by different regulatory mechanisms across cell types.
Gratifyingly, this complex regulation defines simple rules ⬇️
www.biorxiv.org/content/10.1...
The regulation of most proteins is dominated by different regulatory mechanisms across cell types.
Gratifyingly, this complex regulation defines simple rules ⬇️
www.biorxiv.org/content/10.1...
September 22, 2025 at 10:54 AM
Some proteins are primarily regulated by one mechanism: RNA abundance, translation, or clearance.
The regulation of most proteins is dominated by different regulatory mechanisms across cell types.
Gratifyingly, this complex regulation defines simple rules ⬇️
www.biorxiv.org/content/10.1...
The regulation of most proteins is dominated by different regulatory mechanisms across cell types.
Gratifyingly, this complex regulation defines simple rules ⬇️
www.biorxiv.org/content/10.1...
We quantified mRNA abundance, translation, protein abundance, protein degradation and cell growth across thousands of single cells from a mammalian tissue.
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
September 21, 2025 at 11:07 AM
We quantified mRNA abundance, translation, protein abundance, protein degradation and cell growth across thousands of single cells from a mammalian tissue.
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles:
www.biorxiv.org/content/10.1...
🧵
A key mechanism controlling mitosis is the precise timing of over 32,000 phosphorylation events.
Below are some of the key regulators of mitosis.
Below are some of the key regulators of mitosis.
September 11, 2025 at 11:19 AM
A key mechanism controlling mitosis is the precise timing of over 32,000 phosphorylation events.
Below are some of the key regulators of mitosis.
Below are some of the key regulators of mitosis.