Dave Rhee
daveyrhee.bsky.social
Dave Rhee
@daveyrhee.bsky.social
Cell Biologist | E3 ligases | Molecular glues
Reposted by Dave Rhee
New work @harvard by Miguel Gonzalez-Lozano @harperlabhms.bsky.social & @ernstschmid.bsky.social in Johannes Walter lab charts structural interactome of endosomes. #XL-MS #Alphafold Funded by @asapresearch.parkinsonsroadmap.org & NIH. Science continues-despite attacks www.nature.com/articles/s41...
EndoMAP.v1 charts the structural landscape of human early endosome complexes - Nature
A study presents EndoMAP.v1, a resource that combines information on protein interactions and crosslink-supported structural predictions to map the interaction landscape of early endosomes.
www.nature.com
May 28, 2025 at 3:52 PM
Reposted by Dave Rhee
UBQLN2 facilitates degradation of the retrotransposon protein PEG10 via UBE3A activity https://www.biorxiv.org/content/10.1101/2025.03.28.646022v1
March 31, 2025 at 7:30 AM
Reposted by Dave Rhee
New @biorxivpreprint.bsky.social preprint from Amgen induced proximity group and @dannomura.bsky.social lab just posted that describes Covalent Destabilizing Degrader of AR and AR-V7 in Androgen-Independent Prostate Cancer Cells. Read on for an overview 1/7 www.biorxiv.org/content/10.1...
Covalent Destabilizing Degrader of AR and AR-V7 in Androgen-Independent Prostate Cancer Cells
Androgen-independent prostate cancers, correlated with heightened aggressiveness and poor prognosis, are caused by mutations or deletions in the androgen receptor (AR) or expression of truncated varia...
www.biorxiv.org
February 18, 2025 at 7:18 PM
Reposted by Dave Rhee
Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...
EndoMAPV1, a Structural Protein Complex Landscape of Human Endosomes
Early/sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome1,2,3. These events are c...
www.biorxiv.org
February 11, 2025 at 11:38 PM
Reposted by Dave Rhee
Check out our recent efforts in chemrxiv to expand the chemical tractability of the proteome through NP-inspired photoaffinity probes

chemrxiv.org/engage/chemr...
February 5, 2025 at 7:46 AM
Reposted by Dave Rhee
🎉Super excited to share our story on how the substrate receptor FBXO31 functions as a quality control factor by recognizing amides. This has been an amazing collaboration between Bode lab and @jcornlab.bsky.social. Special shutout goes to @matthiasmuhar.bsky.social www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
www.nature.com
January 29, 2025 at 4:29 PM
Reposted by Dave Rhee
New to #ubiquitin? Been with it for a while? Either way this review may be for you 🤩

Excited to share our review article in @molcell.bsky.social, diving deep into everything #ubiquitin
Read here 👉: kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...

@cellpress.bsky.social
@wehi-research.bsky.social
January 17, 2025 at 2:19 AM
Reposted by Dave Rhee
Really pleased to share this story from Amphista Therapeutics on the discovery and MoA of a potent and selective BRD9 "targeted glue" degrader. The compound also works in vivo and is orally bioavailable! Fantastic work from the team on a super fun project!
www.biorxiv.org/content/10.1...
January 2, 2025 at 4:26 PM
Reposted by Dave Rhee
🎄 xmas preprint 🎄 we are excited to share our cryo-EM structure of UBR4 in complex with KCMF1 and CALM1. the PQC ligase forms a massive ubiquitination arena, primed to amplify ubiquitin chains (E4 activity) and boost degradation of defective proteins. www.biorxiv.org/content/10.1...
December 21, 2024 at 10:46 AM
Reposted by Dave Rhee
#Technology Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes by @d_ogasa, @brumelillo, @bencravatt at @scrippsresearch @broadinstitute @harvardmed @MassGeneralNews http://dlvr.it/TGwBJt
December 20, 2024 at 7:01 PM
Reposted by Dave Rhee
Excited to reveal specific reactive oxygen species as sentinel molecules that control localized protein degradation at #mitochondria to adjust electron transport chain abundance, with many implications for the role of ROS in development and disease.

www.cell.com/molecular-ce...
Reactive oxygen species control protein degradation at the mitochondrial import gate
This work identifies reactive oxygen species released by the electron transport chain as sentinel molecules that regulate localized protein degradation at mitochondrial TOM complexes, a mechanism that controls mitochondrial protein import and adjusts the abundance of the electron transport chain to cellular needs.
www.cell.com
December 6, 2024 at 7:07 AM
Reposted by Dave Rhee
We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! www.biorxiv.org/content/10.1... 🧵 1/6
A multivalent adaptor mechanism drives the nuclear import of proteasomes
Nuclear protein homeostasis, including the turnover of transcription factors, critically depends on nuclear proteasomes. After each cell division, proteasomes need to be re-imported into the newly for...
www.biorxiv.org
December 5, 2024 at 8:11 PM
Reposted by Dave Rhee
Excited to share our latest preprint on @biorxivpreprint.bsky.social: "The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro".

www.biorxiv.org/content/10.1...
The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro
HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin chain assembly complex (LUBAC). While most E3 ubiquitin ligases conjugate ubiquitin to protein lysi...
www.biorxiv.org
December 3, 2024 at 11:54 PM
Reposted by Dave Rhee
Very excited to present the structural and mechanistic basis for stress response silencing by the monster E3 ligase SIFI.

www.biorxiv.org/content/10.1...
December 3, 2024 at 7:05 AM
Reposted by Dave Rhee
There are no shortcuts in drug discovery. And it takes incredible efforts to make sense of what you can find in the scientific literature. This is an excellent piece of work to quality check what has been claimed as a new avenue in targeted protein degradation (TPD). www.nature.com/articles/s41...
Critical assessment of LC3/GABARAP ligands used for degrader development and ligandability of LC3/GABARAP binding pockets - Nature Communications
Autophagosome tethering compounds (ATTECs) are small molecule degraders hijacking the autophagy system. Here, the authors show that current ATTEC ligands did not bind to their designated targets but e...
www.nature.com
November 25, 2024 at 10:09 PM
Reposted by Dave Rhee
Excited to report our discovery of an important component of the oxidative stress response: the E3 ligase TRIP12. It acts as a chain elongation factor that amplifies CUL3-KEAP1 activity to drive NRF2 degradation as cells recover from stress.

www.biorxiv.org/content/10.1...
Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12
The oxidative stress response is centered on the transcription factor NRF2 and protects cells from reactive oxygen species (ROS). While ROS inhibit the E3 ligase CUL3-KEAP1 to stabilize NRF2 and elici...
www.biorxiv.org
November 26, 2024 at 2:53 PM
Reposted by Dave Rhee
Reposted by Dave Rhee
Crystal structures of DCAF1-PROTAC-WDR5 ternary complexes provide insight into DCAF1 substrate specificity www.nature.com/articles/s41...
Crystal structures of DCAF1-PROTAC-WDR5 ternary complexes provide insight into DCAF1 substrate specificity - Nature Communications
The authors show that DCAF1, a substrate receptor of CUL4 and EDVP E3 ligases, can be recruited by PROTACs to degrade the cancer drug target, WDR5. They also report the crystal structures of PROTAC te...
www.nature.com
November 25, 2024 at 8:31 AM
Reposted by Dave Rhee
go.bsky.app/3VPQofn
Have a look at the Ubiquitin and Ubls list Lisa :-)
November 20, 2024 at 1:24 PM
Reposted by Dave Rhee
New work from Brenda Schulman Lab (@mpi-biochem), Gary Kleiger (UnivNevada), @harperlabhms.bsky.social , & Elledge Lab (HMS) reveal combinatorial mechanisms of substrate specificity by a family of KLDC C-degron ubiquitin ligases. Led by Danny Scott (St Jude). www.nature.com/articles/s41...
Structural basis for C-degron selectivity across KLHDCX family E3 ubiquitin ligases - Nature Communications
Kelch-domain KLHDCX E3 ligases bind substrate C-terminal glycines. This study reveals substrate selectivity by E3s with similar structures; C-degrons are perceived by a “C-terminus anchor motif”, whos...
www.nature.com
November 16, 2024 at 2:58 PM