Bobby Hollingsworth
@bobbyhollingsworth.bsky.social
Senior Sci, Arena BioWorks | Postdoc @harperlabhms.bsky.social 24' | Ph.D. @BBS_Harvard @WuLabHarvard '21 | @VTEngineering '17 | Cell Biology | Cryo-EM | Innate immunity | He/him | Views = own | @BobbyHollings
Pinned
Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists · Wade Harper Lab @ HMS Cell Biology
harperlab.pubpub.org
I am excited to share my first postdoc project in with the community for feedback. We used spatiotemporal proteomics (1) to describe how cells respond to NLRP3 agonists and (2) to profile the proximal proteome as NLRP3 trafficks to the site of inflammasome nucleation harperlab.pubpub.org/pub/nlrp3/
Reposted by Bobby Hollingsworth
Today, my NIH colleagues and I did something scary but really important. We spoke up about egregious harms we are seeing happen to research participants and public health through thoughtless and politicized policies at NIH. 🧵1/3
apnews.com/article/nih-...
apnews.com/article/nih-...
June 10, 2025 at 4:10 AM
Today, my NIH colleagues and I did something scary but really important. We spoke up about egregious harms we are seeing happen to research participants and public health through thoughtless and politicized policies at NIH. 🧵1/3
apnews.com/article/nih-...
apnews.com/article/nih-...
This is awesome! I've been hoping to see some sort of FKBP/SBP-hook/localization sequence approach to better understand whether the biophysical properties of membrane clustering promote inflammasome assembly. Useful insights towards finding the minimally sufficient activation sequence!
Ever wondered where NLRP3 needs to be in the cell to activate the #inflammasome? Turns out, it does not really matter. Check out our latest study in
@natcomms.nature.com: nature.com/articles/s41...
See 🧵👇
1/9
@natcomms.nature.com: nature.com/articles/s41...
See 🧵👇
1/9
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly - Nature Communications
NLRP3 inflammasome activation is critical for the induction of protective immunity, but molecular insights are still lacking. Here, the authors express NLRP3 variants targeted to different cellular lo...
nature.com
June 5, 2025 at 9:42 PM
This is awesome! I've been hoping to see some sort of FKBP/SBP-hook/localization sequence approach to better understand whether the biophysical properties of membrane clustering promote inflammasome assembly. Useful insights towards finding the minimally sufficient activation sequence!
This might be the only issue where I find common ground with RFK Jr: the for-profit journal system warps the incentives driving science, with serious consequences. Still, it does not come close to offset the harm he's done to U.S. biomedical research.
RFK Jr. plans to bar NIH scientists from top journals like NEJM & JAMA, replacing them with government-run outlets.
This would delegitimize taxpayer-funded research, warns Dr. Adam Gaffney of Harvard Medical School.
www.washingtonpost.com/health/2025/...
This would delegitimize taxpayer-funded research, warns Dr. Adam Gaffney of Harvard Medical School.
www.washingtonpost.com/health/2025/...
RFK Jr. says he may bar scientists from publishing in top medical journals
Health Secretary Robert F. Kennedy Jr. took aim at reputed journals such as the Lancet and said his agency will create “in-house” publications instead.
www.washingtonpost.com
May 28, 2025 at 10:24 PM
This might be the only issue where I find common ground with RFK Jr: the for-profit journal system warps the incentives driving science, with serious consequences. Still, it does not come close to offset the harm he's done to U.S. biomedical research.
Reposted by Bobby Hollingsworth
The NYT wants you to think universities became dependent on government. This is false.
The government invested in the national research infrastructure by competitive grants to universities. The taxpayers built this. It got us to the moon. Won the Cold War. Created Silicon Valley. Made the McRib.
The government invested in the national research infrastructure by competitive grants to universities. The taxpayers built this. It got us to the moon. Won the Cold War. Created Silicon Valley. Made the McRib.
April 19, 2025 at 11:20 PM
The NYT wants you to think universities became dependent on government. This is false.
The government invested in the national research infrastructure by competitive grants to universities. The taxpayers built this. It got us to the moon. Won the Cold War. Created Silicon Valley. Made the McRib.
The government invested in the national research infrastructure by competitive grants to universities. The taxpayers built this. It got us to the moon. Won the Cold War. Created Silicon Valley. Made the McRib.
Reposted by Bobby Hollingsworth
From Nobel prize winning cancer researcher Bill Kaelin
Killing the Science Golden Goose - The American Journal of Medicine www.amjmed.com/article/S000...
Killing the Science Golden Goose - The American Journal of Medicine www.amjmed.com/article/S000...
Killing the Science Golden Goose
In the 1970’s I was probably one of the last American high school students who was
told that if you wanted to go to medical school, you should learn to speak German.
This was because in the late 19th ...
www.amjmed.com
April 18, 2025 at 10:54 AM
From Nobel prize winning cancer researcher Bill Kaelin
Killing the Science Golden Goose - The American Journal of Medicine www.amjmed.com/article/S000...
Killing the Science Golden Goose - The American Journal of Medicine www.amjmed.com/article/S000...
Reposted by Bobby Hollingsworth
Way to go Cornell!!
Cornell too!
www.cornellsun.com/article/2025...
www.cornellsun.com/article/2025...
Cornell Sues DOE for ‘Unlawful’ Cuts to Indirect Costs for Research Grants
University administrators announced in a Monday statement that Cornell joined a lawsuit against the U.S. Department of Energy and DOE Secretary Chris Wright which cut research grant funding for indire...
www.cornellsun.com
April 14, 2025 at 10:08 PM
Way to go Cornell!!
Reposted by Bobby Hollingsworth
"The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage" out now in Nature Cell Biology
rdcu.be/ehbaL
rdcu.be/ehbaL
The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage
Nature Cell Biology - Wang et al. show the recruitment of the lipid channel protein VPS13C and formation of VPS13C-dependent contacts between endoplasmic reticulum and lysosomes after lysosomal...
rdcu.be
April 10, 2025 at 2:17 PM
"The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage" out now in Nature Cell Biology
rdcu.be/ehbaL
rdcu.be/ehbaL
Reposted by Bobby Hollingsworth
We called out Harvard in the past for inaction. So now we are happy to speak out with praise.
This is a major step forward.
It’s not just a lack of capitulation. It’s an aggressive effort leaning into public comms — exactly what is needed. Bravo to Harvard 👏
This is a major step forward.
It’s not just a lack of capitulation. It’s an aggressive effort leaning into public comms — exactly what is needed. Bravo to Harvard 👏
Harvard redid its whole homepage to push back against the administration’s demands. I mean, this is just a website but I think it’s kind of a great PR move: www.harvard.edu
Harvard University
Harvard University is devoted to excellence in teaching, learning, and research, and to developing leaders who make a difference globally.
www.harvard.edu
April 14, 2025 at 10:50 PM
We called out Harvard in the past for inaction. So now we are happy to speak out with praise.
This is a major step forward.
It’s not just a lack of capitulation. It’s an aggressive effort leaning into public comms — exactly what is needed. Bravo to Harvard 👏
This is a major step forward.
It’s not just a lack of capitulation. It’s an aggressive effort leaning into public comms — exactly what is needed. Bravo to Harvard 👏
Excited to see this reporter characterized in more depth after its initial use awhile back--works well and I deposited a few StayGold versions of it on Addgene in the meantime (#228044, #228043)
April 6, 2025 at 4:44 PM
Excited to see this reporter characterized in more depth after its initial use awhile back--works well and I deposited a few StayGold versions of it on Addgene in the meantime (#228044, #228043)
Reposted by Bobby Hollingsworth
🎉 This month, we are recognizing Felix Kraus from CRN Team Harper as our #OpenScience Champion for ensuring all research outputs associated with his recent publication were shared in public repositories. Thank you for all your hard work, Felix!
Check out his recent #publication: shorturl.at/YebyC
Check out his recent #publication: shorturl.at/YebyC
March 27, 2025 at 3:59 PM
🎉 This month, we are recognizing Felix Kraus from CRN Team Harper as our #OpenScience Champion for ensuring all research outputs associated with his recent publication were shared in public repositories. Thank you for all your hard work, Felix!
Check out his recent #publication: shorturl.at/YebyC
Check out his recent #publication: shorturl.at/YebyC
Reposted by Bobby Hollingsworth
From 2010 to 2016 (latest data I have ), NIH research contributed to EVERY drug approved by the FDA
March 22, 2025 at 10:44 AM
From 2010 to 2016 (latest data I have ), NIH research contributed to EVERY drug approved by the FDA
Reposted by Bobby Hollingsworth
I’m a biomedical researcher who lost his wife to cancer. Finding cures is personal to me. What’s happening at the NIH is devastating. Here’s my editorial in my hometown newspaper.
www.vindy.com/opinion/edit...
www.vindy.com/opinion/edit...
DOGE ends America’s golden age of biomedical research
Biomedical research impacts all of us. I became curious about how drugs work after my mom was diagnosed with rheumatoid arthritis when I was a small boy. She received “gold shots” as treatment, which ...
www.vindy.com
March 17, 2025 at 8:34 PM
I’m a biomedical researcher who lost his wife to cancer. Finding cures is personal to me. What’s happening at the NIH is devastating. Here’s my editorial in my hometown newspaper.
www.vindy.com/opinion/edit...
www.vindy.com/opinion/edit...
Research universities/admin/faculty need to choose whether to stand with Columbia through actions like walkouts or risk ceding free academic speech forever and becoming future targets for federal retribution at a whim.
March 11, 2025 at 5:07 PM
Research universities/admin/faculty need to choose whether to stand with Columbia through actions like walkouts or risk ceding free academic speech forever and becoming future targets for federal retribution at a whim.
Reposted by Bobby Hollingsworth
My latest post is now out.
I show how Trump's attacks on science and universities are neither random nor new - they fit very precisely into the authoritarian playbook.
This means we can guess what might come next and prepare - and we must!
christinapagel.substack.com/p/censor-pur...
I show how Trump's attacks on science and universities are neither random nor new - they fit very precisely into the authoritarian playbook.
This means we can guess what might come next and prepare - and we must!
christinapagel.substack.com/p/censor-pur...
Censor, purge, defund: how Trump following the authoritarian playbook on science and universities
I have mapped 35 of the Trump administration's attacks on science and universities to the authoritarian playbook - and consider what it means for attacks still to come
christinapagel.substack.com
March 10, 2025 at 2:58 PM
My latest post is now out.
I show how Trump's attacks on science and universities are neither random nor new - they fit very precisely into the authoritarian playbook.
This means we can guess what might come next and prepare - and we must!
christinapagel.substack.com/p/censor-pur...
I show how Trump's attacks on science and universities are neither random nor new - they fit very precisely into the authoritarian playbook.
This means we can guess what might come next and prepare - and we must!
christinapagel.substack.com/p/censor-pur...
Reposted by Bobby Hollingsworth
Welcome to the Bluesky account for Stand Up for Science 2025!
Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide!
#scienceforall #sciencenotsilence
Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide!
#scienceforall #sciencenotsilence
February 12, 2025 at 5:04 PM
Welcome to the Bluesky account for Stand Up for Science 2025!
Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide!
#scienceforall #sciencenotsilence
Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide!
#scienceforall #sciencenotsilence
Reposted by Bobby Hollingsworth
BREAKING: We're sending an open letter to universities across the country, offering support and urging them to reject pressure to punish international students and faculty for exercising their First Amendment rights.
Schools must hold firm against the Trump administration's censorship attempts.
Schools must hold firm against the Trump administration's censorship attempts.
March 4, 2025 at 8:05 PM
BREAKING: We're sending an open letter to universities across the country, offering support and urging them to reject pressure to punish international students and faculty for exercising their First Amendment rights.
Schools must hold firm against the Trump administration's censorship attempts.
Schools must hold firm against the Trump administration's censorship attempts.
Reposted by Bobby Hollingsworth
AACR urges Congress to reaffirm its long history of steadfast and bipartisan support for medical research by ensuring that NIH has all of the resources needed to advance its lifesaving mission to prevent, diagnose, and treat cancer and other diseases: AACR.org/NIH
February 18, 2025 at 6:10 PM
AACR urges Congress to reaffirm its long history of steadfast and bipartisan support for medical research by ensuring that NIH has all of the resources needed to advance its lifesaving mission to prevent, diagnose, and treat cancer and other diseases: AACR.org/NIH
Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...
EndoMAPV1, a Structural Protein Complex Landscape of Human Endosomes
Early/sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome1,2,3. These events are c...
www.biorxiv.org
February 11, 2025 at 11:38 PM
Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...
Reposted by Bobby Hollingsworth
A large dataset we hope is helpful for patients who may have CAPS, an #autoinflammatory condition associated with genetic variants in NLRP3. Lots of "variants of uncertain significance" now have a specific readout from our in vitro assay which can help with clinical diagnosis.
rdcu.be/d9iRG
rdcu.be/d9iRG
Mechanisms of NLRP3 activation and inhibition elucidated by functional analysis of disease-associated variants
Nature Immunology - Gain-of-function variants in the gene encoding NLRP3 lead to constitutive inflammasome activation and excessive IL-1β production. In this resource, authors perform...
rdcu.be
February 11, 2025 at 9:57 AM
A large dataset we hope is helpful for patients who may have CAPS, an #autoinflammatory condition associated with genetic variants in NLRP3. Lots of "variants of uncertain significance" now have a specific readout from our in vitro assay which can help with clinical diagnosis.
rdcu.be/d9iRG
rdcu.be/d9iRG
Reposted by Bobby Hollingsworth
Please join my cell biology colleagues and CALL Congress. emails are not as impactful. Here is a simple sample script
![“Hello, my name is [Dr. Jim Smith], and I live on [Main Street in Anytown, Connecticut]. I am a [biomedical researcher at the State University], where I study [briefly describe your research]. This research is funded by the [NIH] and is crucial because [in one sentence explain why your research is important to the public].
The decision by the NIH to immediately reduce the amount of funds it provides universities to support biomedical research will have a devastating impact on American science.
Universities are vital partners in the research we do. Without the administrative support and the research facilities they provide, I could not do my research.
I am deeply concerned about the implications of this decision on America’s standing as the world’s leader in life science research.
I urge [Senator Jones] to take action to stop these reductions in university research support. Thank you for your time, and I would be happy to discuss this further at any time.”](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:r3avzdjyhu6jm7vxia265ncu/bafkreic44hnsrs4r5z7wf2vbebjkcgmq27uecx5qekvsc3jnk4endtunbi@jpeg)
February 10, 2025 at 7:49 PM
Please join my cell biology colleagues and CALL Congress. emails are not as impactful. Here is a simple sample script
Reposted by Bobby Hollingsworth
March 7. Stand Up For Science. Rallies in DC and nationwide. 🧪
Please visit the website and spread the word.
www.standupforscience2025.org
Please visit the website and spread the word.
www.standupforscience2025.org
February 11, 2025 at 1:12 PM
March 7. Stand Up For Science. Rallies in DC and nationwide. 🧪
Please visit the website and spread the word.
www.standupforscience2025.org
Please visit the website and spread the word.
www.standupforscience2025.org
Reposted by Bobby Hollingsworth
Deadline extended! Join us!
Calling all Bay Area researchers for a paid opportunity: the @asterainstitute.bsky.social Open Science Program’s User Testing Day in Emeryville on Feb 11!
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
Application to the Astera Open Science program's user testing day
Are you a Bay Area researcher in academia or industry? Do you want to make an impact on innovative tools designed to transform the way we work and think? Apply below to be a part of the Astera Open Sc...
forms.gle
February 5, 2025 at 11:11 PM
Deadline extended! Join us!
Reposted by Bobby Hollingsworth
Curious about new tools to manage scientific information? Join us at the Whitehead on Tuesday 1/28 2-4pm...💡 @ronent.bsky.social @iaincheeseman.bsky.social @nadjaohnadja.bsky.social #openscience
January 23, 2025 at 1:27 PM
Curious about new tools to manage scientific information? Join us at the Whitehead on Tuesday 1/28 2-4pm...💡 @ronent.bsky.social @iaincheeseman.bsky.social @nadjaohnadja.bsky.social #openscience
Reposted by Bobby Hollingsworth
Time to share our organelle proteome paper
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/
1/3 🧪
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/
1/3 🧪
January 14, 2025 at 4:03 PM
Time to share our organelle proteome paper
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/
1/3 🧪
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/
1/3 🧪