Alex Ludwig
@alexludwig.bsky.social
Cell biologist and nature lover interested in epithelial cell junctions, mechanobiology, and cell polarity. Asst Prof at NTU Singapore.
Check out the ALab homepage:
https://blogs.ntu.edu.sg/alabntusg/
Check out the ALab homepage:
https://blogs.ntu.edu.sg/alabntusg/
An Archaea bacterium with an internal membrane system! Amazing and beautiful work!
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
November 8, 2025 at 12:47 AM
An Archaea bacterium with an internal membrane system! Amazing and beautiful work!
Reposted by Alex Ludwig
1/11 In @science.org: A new perspective on how our intestines renew. Cells are not “pushed out” by crowding or die from apoptosis. Instead, cells play a mechanical tug-of-war, where weaker cells extrude, reframing gut renewal as force-regulated. www.science.org/doi/10.1126/...
Epithelial tension controls intestinal cell extrusion
Cell extrusion is essential for homeostatic self-renewal of the intestinal epithelium. Extrusion is thought to be triggered by crowding-induced compression of cells at the intestinal villus tip. In th...
www.science.org
September 6, 2025 at 7:36 PM
1/11 In @science.org: A new perspective on how our intestines renew. Cells are not “pushed out” by crowding or die from apoptosis. Instead, cells play a mechanical tug-of-war, where weaker cells extrude, reframing gut renewal as force-regulated. www.science.org/doi/10.1126/...
Reposted by Alex Ludwig
Supracellular actomyosin assemblies: master coordinators of development
In this Review, Katja Röper @katjaroeper.bsky.social discusses supracellular actomyosin structures, tissues where they function, their known roles as well as potential properties
journals.biologists.com/dev/article/...
In this Review, Katja Röper @katjaroeper.bsky.social discusses supracellular actomyosin structures, tissues where they function, their known roles as well as potential properties
journals.biologists.com/dev/article/...
September 1, 2025 at 2:46 PM
Supracellular actomyosin assemblies: master coordinators of development
In this Review, Katja Röper @katjaroeper.bsky.social discusses supracellular actomyosin structures, tissues where they function, their known roles as well as potential properties
journals.biologists.com/dev/article/...
In this Review, Katja Röper @katjaroeper.bsky.social discusses supracellular actomyosin structures, tissues where they function, their known roles as well as potential properties
journals.biologists.com/dev/article/...
Reposted by Alex Ludwig
Our latest efforts to understand Enterococcal wound infection. In long-term collaboration with @gthibault.bsky.social, we discovered how E. faecalis makes extracellular ROS - via EET!⚡️Which in turn dysregulates host UPR to delay wound healing. Led by @aarontan.bsky.social - his videos below are 🤩!
📢 Our new preprint is out! We show how the common gut bacterium 𝘌. 𝘧𝘢𝘦𝘤𝘢𝘭𝘪𝘴 stops wound healing. It uses a metabolic pathway, extracellular electron transport (EET), to stress host cells, halting their migration. Watch WT bacteria (orange) stop the cells while the mutant (blue) doesn't!
August 11, 2025 at 3:51 AM
Our latest efforts to understand Enterococcal wound infection. In long-term collaboration with @gthibault.bsky.social, we discovered how E. faecalis makes extracellular ROS - via EET!⚡️Which in turn dysregulates host UPR to delay wound healing. Led by @aarontan.bsky.social - his videos below are 🤩!
We have some news! We find that the post-synaptic Homer proteins coordinate YAP and Wnt signaling in epithelial cells, downstream of the Crumbs polarity complex. They do so by sequestering YAP and beta-catenin in biomolecular condensates, and by suppressing a FRYL/NDR complex. Check it out!
Homer condensates orchestrate YAP-Wnt signaling crosstalk downstream of the Crumbs polarity complex https://www.biorxiv.org/content/10.1101/2025.07.23.666266v1
July 25, 2025 at 1:23 PM
We have some news! We find that the post-synaptic Homer proteins coordinate YAP and Wnt signaling in epithelial cells, downstream of the Crumbs polarity complex. They do so by sequestering YAP and beta-catenin in biomolecular condensates, and by suppressing a FRYL/NDR complex. Check it out!
Reposted by Alex Ludwig
Announcing a new @embo.org Workshop on "Intracellular mechanics and organelle mechanobiology" that we are organizing with Michael Krieg and Verena Ruprecht at @icfo.eu (Barcelona) on 16-20 February, 2026. Please, repost and spread the word! 🙏 #EMBOmechanobio
meetings.embo.org/event/26-org...
meetings.embo.org/event/26-org...
Intracellular mechanics and organelle mechanobiology
Mechanobiology is an interdisciplinary field that emerges at the cross-section of biology, physics and engineering. It aims to understand how living cells, tissues and animals sense and respond to me…
meetings.embo.org
July 4, 2025 at 3:50 PM
Announcing a new @embo.org Workshop on "Intracellular mechanics and organelle mechanobiology" that we are organizing with Michael Krieg and Verena Ruprecht at @icfo.eu (Barcelona) on 16-20 February, 2026. Please, repost and spread the word! 🙏 #EMBOmechanobio
meetings.embo.org/event/26-org...
meetings.embo.org/event/26-org...
Reposted by Alex Ludwig
How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp
April 14, 2025 at 11:58 AM
How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp
Our paper on the regulation of the tight junction leak pathway by Arhgap12/N-Wasp is out ! www.cell.com/cell-reports...
ARHGAP12 suppresses F-actin assembly to control epithelial tight junction mechanics and paracellular leak pathway permeability
Tambrin et al. show that the Cdc42/Rac GAP ARHGAP12 promotes the flux of macromolecules
across tight junctions by suppressing junctional actin assembly and tension via N-WASP
and the Arp2/3 complex. T...
www.cell.com
April 8, 2025 at 6:22 AM
Our paper on the regulation of the tight junction leak pathway by Arhgap12/N-Wasp is out ! www.cell.com/cell-reports...
We have some exciting news about how epithelial cells initiate an apical lumen de novo, and how this is coordinated with cell-cell junction formation. Check it out: www.biorxiv.org/content/10.1...
Bulk exocytosis of large intracellular apical precursor organelles establishes apical domain identity during de novo lumen formation
The creation of a microvilli-rich apical luminal domain is a key event in the development of epithelial tissues. De novo lumenogenesis, in which epithelial cells establish apical identity by directing...
www.biorxiv.org
March 7, 2025 at 7:41 AM
We have some exciting news about how epithelial cells initiate an apical lumen de novo, and how this is coordinated with cell-cell junction formation. Check it out: www.biorxiv.org/content/10.1...
Another beautiful tight junction. This time with scale bar :)
February 6, 2025 at 1:47 PM
Another beautiful tight junction. This time with scale bar :)
Not bad for a conventionally fixed and dehydrated TEM sample. No HPF/FS. OTO method. Stains the kissing points quite nicely.
February 2, 2025 at 9:59 AM
Not bad for a conventionally fixed and dehydrated TEM sample. No HPF/FS. OTO method. Stains the kissing points quite nicely.
Reposted by Alex Ludwig
First official post! A new paper out about #cytokinesis in #epithelia
www.embopress.org/doi/full/10....
Explore our journey as we uncover how regulators of cell-cell and cell-matrix interactions modulate cytokinesis efficiency, while revealing new roles for the Dystrophin-Dystroglycan complex.
www.embopress.org/doi/full/10....
Explore our journey as we uncover how regulators of cell-cell and cell-matrix interactions modulate cytokinesis efficiency, while revealing new roles for the Dystrophin-Dystroglycan complex.
November 15, 2024 at 6:41 PM
First official post! A new paper out about #cytokinesis in #epithelia
www.embopress.org/doi/full/10....
Explore our journey as we uncover how regulators of cell-cell and cell-matrix interactions modulate cytokinesis efficiency, while revealing new roles for the Dystrophin-Dystroglycan complex.
www.embopress.org/doi/full/10....
Explore our journey as we uncover how regulators of cell-cell and cell-matrix interactions modulate cytokinesis efficiency, while revealing new roles for the Dystrophin-Dystroglycan complex.
This is sabotage! And this may well be just the beginning.
January 23, 2025 at 12:31 AM
This is sabotage! And this may well be just the beginning.
You don’t have to be German to know what this stands for - it’s clear as mud - and it’s disgusting!
Its not a gaffe. It's back to back, clear as day Nazi salutes.
And how does this not make sense? Did we not just watch the campaign Trump ran? Have we all not seen the antisemitism in MAGA?
Feels like this is all we should be talking about tomorrow.
And how does this not make sense? Did we not just watch the campaign Trump ran? Have we all not seen the antisemitism in MAGA?
Feels like this is all we should be talking about tomorrow.
January 21, 2025 at 2:55 PM
You don’t have to be German to know what this stands for - it’s clear as mud - and it’s disgusting!
Reposted by Alex Ludwig
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade https://www.biorxiv.org/content/10.1101/2025.01.10.632381v1
January 11, 2025 at 8:46 AM
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade https://www.biorxiv.org/content/10.1101/2025.01.10.632381v1
Reposted by Alex Ludwig
Our #CryoET paper on ciliary rootlet ultrastructure from mouse retina is now out in @elife.bsky.social! Have a peek with this video or dive deeper at bit.ly/3ZJzhgc. Big thanks to @carter-lab.bsky.social and #teamtomo.
#CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science
#CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science
December 8, 2024 at 2:38 PM
Our #CryoET paper on ciliary rootlet ultrastructure from mouse retina is now out in @elife.bsky.social! Have a peek with this video or dive deeper at bit.ly/3ZJzhgc. Big thanks to @carter-lab.bsky.social and #teamtomo.
#CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science
#CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science
Mind blowing! Look at those nuclear pores.
ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY.
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Ångström-resolution fluorescence microscopy - Nature
The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...
www.nature.com
November 29, 2024 at 12:10 AM
Mind blowing! Look at those nuclear pores.
Still one of my favourite tomograms, recorded ages ago at NCMIR (UCSD). It shows interconnected networks of caveolae (labeled with a miniSOG probe) at the rear of RPE1 cells. Bummer we so far haven't been able to image such networks in larger cell volumes; it's a lot of membrane that's stored here.
November 16, 2024 at 3:42 AM
Still one of my favourite tomograms, recorded ages ago at NCMIR (UCSD). It shows interconnected networks of caveolae (labeled with a miniSOG probe) at the rear of RPE1 cells. Bummer we so far haven't been able to image such networks in larger cell volumes; it's a lot of membrane that's stored here.
Woah. Bluesky seems to be much more than a much needed alternative to X. I didn't expect this big a science community here tbh. I love the start up packages; really cool to connect with so many people I never met or even heard of. Since I'm new to this, I'll briefly introduce my lab and what we do.
November 13, 2024 at 6:15 AM
Woah. Bluesky seems to be much more than a much needed alternative to X. I didn't expect this big a science community here tbh. I love the start up packages; really cool to connect with so many people I never met or even heard of. Since I'm new to this, I'll briefly introduce my lab and what we do.
This will be a fantastic meeting! Looking forward to it
Cell and developmental biologists--Join us at the 2025 Cell Contact and Adhesion Gordon Conference. We've assembled a strong and diverse program of speakers at the cutting-edge of science in our field 1/n Please repost
www.grc.org/cell-contact...
www.grc.org/cell-contact...
November 12, 2024 at 1:45 AM
This will be a fantastic meeting! Looking forward to it