Kimberly Kline 🏔
@kimingeneva.bsky.social
Professor, University of Geneva. We study Enterococcal biofilms, pathogenesis, and AMR. Formerly @KimInSingapore 🌴.
https://kimberlyklinelab.com/
https://kimberlyklinelab.com/
Pinned
Kimberly Kline 🏔
@kimingeneva.bsky.social
· Nov 20
Hey Enterococcus lovers: go.bsky.app/T24bCrJ. Who are we missing here?
Reposted by Kimberly Kline 🏔
'In 2021, 11,021 deaths and 353,634 DALYs were attributable to VREfm worldwide, (...) 2,991 deaths and 82,025 DALYs attributable to VREfs. (...) VREfm burden is projected to continue rising across all indicators by 2050, while VREfs shows a sustained decline.'
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Global burden, inequalities and projections of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis, 1990–2050: a systematic global analysis
Vancomycin-resistant Enterococcus faecium (VREfm) and Enterococcus faecalis (VREfs) are major antimicrobial resistance threats. Previous assessments r…
www.sciencedirect.com
December 19, 2025 at 5:39 PM
'In 2021, 11,021 deaths and 353,634 DALYs were attributable to VREfm worldwide, (...) 2,991 deaths and 82,025 DALYs attributable to VREfs. (...) VREfm burden is projected to continue rising across all indicators by 2050, while VREfs shows a sustained decline.'
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
🧵Enterococcus faecalis is not just an extracellular organism. 🧵 A growing body of work shows it can survive + REPLICATE inside host cells, and that intracellular life may seed persistence, dissemination, and reinfection. 1/n #MMBR @asm.org journals.asm.org/doi/10.1128/...
Enterococcus faecalis: an overlooked cell invader | Microbiology and Molecular Biology Reviews
SUMMARYEnterococcus faecalis and Enterococcus faecium are human pathobionts that exhibit a dual lifestyle as commensal and pathogenic bacteria. The pathogenic lifestyle is associated with specific conditions involving host susceptibility and intestinal ...
journals.asm.org
December 18, 2025 at 9:19 AM
🧵Enterococcus faecalis is not just an extracellular organism. 🧵 A growing body of work shows it can survive + REPLICATE inside host cells, and that intracellular life may seed persistence, dissemination, and reinfection. 1/n #MMBR @asm.org journals.asm.org/doi/10.1128/...
🤩 Long ago in 2021, Irina Afonina saw E. faecalis prophage tail fibers co-purify with membrane vesicles, but we didn't know their fxn: pmc.ncbi.nlm.nih.gov/articles/PMC.... In this new preprint, Mike Gilmore and colleagues show us what the tail fibers (aka efagins) actually do! Super cool work. 🤩
December 16, 2025 at 12:32 PM
🤩 Long ago in 2021, Irina Afonina saw E. faecalis prophage tail fibers co-purify with membrane vesicles, but we didn't know their fxn: pmc.ncbi.nlm.nih.gov/articles/PMC.... In this new preprint, Mike Gilmore and colleagues show us what the tail fibers (aka efagins) actually do! Super cool work. 🤩
Reposted by Kimberly Kline 🏔
Thrilled to share that neutrophils produce extra long fibrillar components named proteoglycofili or PGF. PGF are released by living neutrophils and do not contain DNA. They might look like NETs but are not NETs…
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Proteoglycofili are glycosaminoglycan-containing fibrillar components released by neutrophils to neutralize bacteria
Alternative to the formation of NETs, André et al. describe here that viable neutrophils
may release a fibrillar component named proteoglycofili or PGF upon stimulation. PGF
formation is associated wi...
www.cell.com
November 27, 2025 at 9:01 PM
Thrilled to share that neutrophils produce extra long fibrillar components named proteoglycofili or PGF. PGF are released by living neutrophils and do not contain DNA. They might look like NETs but are not NETs…
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Reposted by Kimberly Kline 🏔
New preprint from our lab www.biorxiv.org/content/10.1...! Andrea Dos Santos and Clément Vulin combine experiments and models showing how adding glucose can strengthen negative interactions between microbial species. This can be used in tandem with antibiotic treatment to inhibit pathogens!
December 2, 2025 at 1:12 PM
New preprint from our lab www.biorxiv.org/content/10.1...! Andrea Dos Santos and Clément Vulin combine experiments and models showing how adding glucose can strengthen negative interactions between microbial species. This can be used in tandem with antibiotic treatment to inhibit pathogens!
Excited to share our first look at Enterococcus faecium infection biology: diabetic wounds are complex, and E. faecium persists despite early immune responses. In diabetic mice, it shows impaired clearance + sustained neutrophil recruitment, worsening healing.
Enterococcus faecium colonization and persistence in a model of diabetic wound infection https://www.biorxiv.org/content/10.1101/2025.11.20.689639v1
November 24, 2025 at 12:37 PM
Excited to share our first look at Enterococcus faecium infection biology: diabetic wounds are complex, and E. faecium persists despite early immune responses. In diabetic mice, it shows impaired clearance + sustained neutrophil recruitment, worsening healing.
Reposted by Kimberly Kline 🏔
Metabolic cross-talk promotes persistence of Enterococcus in a model of polymicrobial catheter-associated urinary tract infection https://www.biorxiv.org/content/10.1101/2025.11.19.689321v1
November 20, 2025 at 7:17 PM
Metabolic cross-talk promotes persistence of Enterococcus in a model of polymicrobial catheter-associated urinary tract infection https://www.biorxiv.org/content/10.1101/2025.11.19.689321v1
Reposted by Kimberly Kline 🏔
Registration is now open for @keystonesymposia.bsky.social "Beyond #Antibiotics: Emerging Strategies Combating #BacterialInfection." Deadline for scholarships and abstract selected short talks is Jan 7! Join us this May for an amazing meeting! keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26
Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection | Keystone Symposia
Join us at the Keystone Symposia on Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection, May 2026, in Breckenridge, with field leaders!
keysym.us
November 19, 2025 at 5:37 PM
Registration is now open for @keystonesymposia.bsky.social "Beyond #Antibiotics: Emerging Strategies Combating #BacterialInfection." Deadline for scholarships and abstract selected short talks is Jan 7! Join us this May for an amazing meeting! keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26
Reposted by Kimberly Kline 🏔
Enterococcus being one step ahead when it comes to stress… once again! Loved figuring this one out🤓
bsky.app/profile/kimi...
bsky.app/profile/kimi...
Why does daptomycin resistance appear so fast in Enterococcus? We finally have a clue.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
November 18, 2025 at 7:37 PM
Enterococcus being one step ahead when it comes to stress… once again! Loved figuring this one out🤓
bsky.app/profile/kimi...
bsky.app/profile/kimi...
Why does daptomycin resistance appear so fast in Enterococcus? We finally have a clue.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
November 17, 2025 at 8:27 PM
Why does daptomycin resistance appear so fast in Enterococcus? We finally have a clue.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
DAP resistance in enterococci pops up quickly. What’s been missing is why resistance-associated membrane changes look the way they do, and why the classic path of mutations is so predictable.
Reposted by Kimberly Kline 🏔
A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae https://www.biorxiv.org/content/10.1101/2025.11.10.687296v1
November 12, 2025 at 2:17 AM
A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae https://www.biorxiv.org/content/10.1101/2025.11.10.687296v1
We're just 6 months away from @keystoneSymposia.bsky.social Beyond #Antibiotics : Emerging Strategies Combating #BacterialInfection, May 2026 in Breckenridge! 👉Scholarship and short talk abstract deadlines are Jan 7! 🧐 keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26
Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection | Keystone Symposia
Join us at the Keystone Symposia on Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection, May 2026, in Breckenridge, with field leaders!
keysym.us
November 4, 2025 at 2:47 PM
We're just 6 months away from @keystoneSymposia.bsky.social Beyond #Antibiotics : Emerging Strategies Combating #BacterialInfection, May 2026 in Breckenridge! 👉Scholarship and short talk abstract deadlines are Jan 7! 🧐 keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26
Reposted by Kimberly Kline 🏔
Postdoc position (please re-post)
A funded postdoctoral position, will open in 2026 to study aspects of Neisseria gonorrhoeae pathogenesis, antigenic variation, genetics, or physiology.
If interested in more information, please contact Hank Seifert
h-seifert@northwestern.edu
#MicroSky
A funded postdoctoral position, will open in 2026 to study aspects of Neisseria gonorrhoeae pathogenesis, antigenic variation, genetics, or physiology.
If interested in more information, please contact Hank Seifert
h-seifert@northwestern.edu
#MicroSky
September 6, 2025 at 7:32 PM
Postdoc position (please re-post)
A funded postdoctoral position, will open in 2026 to study aspects of Neisseria gonorrhoeae pathogenesis, antigenic variation, genetics, or physiology.
If interested in more information, please contact Hank Seifert
h-seifert@northwestern.edu
#MicroSky
A funded postdoctoral position, will open in 2026 to study aspects of Neisseria gonorrhoeae pathogenesis, antigenic variation, genetics, or physiology.
If interested in more information, please contact Hank Seifert
h-seifert@northwestern.edu
#MicroSky
Reposted by Kimberly Kline 🏔
📣 Ready to lead your own lab? NTU Singapore's Nanyang Assistant Professorship offers a 100% paid salary & a S$2.75M start-up package. We're visiting the US this Sept to recruit top talent. Meet us in NYC, Boston & more! #FacultyJobs #TenureTrack
Register HERE: lnkd.in/gSy5qpA7
Register HERE: lnkd.in/gSy5qpA7
August 27, 2025 at 3:24 AM
📣 Ready to lead your own lab? NTU Singapore's Nanyang Assistant Professorship offers a 100% paid salary & a S$2.75M start-up package. We're visiting the US this Sept to recruit top talent. Meet us in NYC, Boston & more! #FacultyJobs #TenureTrack
Register HERE: lnkd.in/gSy5qpA7
Register HERE: lnkd.in/gSy5qpA7
💕 Such a joyful day celebrating the wedding of our longest-standing lab member Pei Yi (since 2012!) and former PhD student Kelvin. 💍 Their special day brought together generations of the Kline Lab family - a wonderful reminder of the friendships and community that science builds along the way. 💕
August 24, 2025 at 3:38 PM
💕 Such a joyful day celebrating the wedding of our longest-standing lab member Pei Yi (since 2012!) and former PhD student Kelvin. 💍 Their special day brought together generations of the Kline Lab family - a wonderful reminder of the friendships and community that science builds along the way. 💕
Our latest efforts to understand Enterococcal wound infection. In long-term collaboration with @gthibault.bsky.social, we discovered how E. faecalis makes extracellular ROS - via EET!⚡️Which in turn dysregulates host UPR to delay wound healing. Led by @aarontan.bsky.social - his videos below are 🤩!
📢 Our new preprint is out! We show how the common gut bacterium 𝘌. 𝘧𝘢𝘦𝘤𝘢𝘭𝘪𝘴 stops wound healing. It uses a metabolic pathway, extracellular electron transport (EET), to stress host cells, halting their migration. Watch WT bacteria (orange) stop the cells while the mutant (blue) doesn't!
August 11, 2025 at 3:51 AM
Our latest efforts to understand Enterococcal wound infection. In long-term collaboration with @gthibault.bsky.social, we discovered how E. faecalis makes extracellular ROS - via EET!⚡️Which in turn dysregulates host UPR to delay wound healing. Led by @aarontan.bsky.social - his videos below are 🤩!
Reposted by Kimberly Kline 🏔
Enterococcus faecalis modulates phase variation in Clostridioides difficile https://www.biorxiv.org/content/10.1101/2025.07.24.666506v1
July 25, 2025 at 4:18 AM
Enterococcus faecalis modulates phase variation in Clostridioides difficile https://www.biorxiv.org/content/10.1101/2025.07.24.666506v1
At my 1st @femsmicro.org congress (in Milan!) - it's a fantastic meeting. I co-chaired a superb session on Microbial Dark Matter with @jlewillett.bsky.social, who also kicked off the session with a talk on her lab's work. 🤩 We also heard from my FEMS Microbes co-EIC Kathleen Scott! 💪🏽 #FEMS2025
July 16, 2025 at 6:21 AM
At my 1st @femsmicro.org congress (in Milan!) - it's a fantastic meeting. I co-chaired a superb session on Microbial Dark Matter with @jlewillett.bsky.social, who also kicked off the session with a talk on her lab's work. 🤩 We also heard from my FEMS Microbes co-EIC Kathleen Scott! 💪🏽 #FEMS2025
Reposted by Kimberly Kline 🏔
Vancomycin-resistant 𝙀𝙣𝙩𝙚𝙧𝙤𝙘𝙤𝙘𝙘𝙪𝙨 𝙛𝙖𝙚𝙘𝙞𝙪𝙢 (VRE) thrives in the antibiotic-perturbed gut
VRE gobbles up enriched sugars and amino acids, and loss of short-chain fatty acids (acetate, propionate, butyrate) eliminates natural growth brakes
Therapeutic angle: Prebiotic SCFA mixtures block VRE growth!
VRE gobbles up enriched sugars and amino acids, and loss of short-chain fatty acids (acetate, propionate, butyrate) eliminates natural growth brakes
Therapeutic angle: Prebiotic SCFA mixtures block VRE growth!
Vancomycin-resistant enterococci utilise antibiotic-enriched nutrients for intestinal colonisation - Nature Communications
Here, the authors show that vancomycin-resistant enterococci grow in the antibiotic-treated gut microbiome by utilising enriched nutrients in the presence of reduced concentrations of inhibitory micro...
www.nature.com
July 11, 2025 at 1:08 PM
Vancomycin-resistant 𝙀𝙣𝙩𝙚𝙧𝙤𝙘𝙤𝙘𝙘𝙪𝙨 𝙛𝙖𝙚𝙘𝙞𝙪𝙢 (VRE) thrives in the antibiotic-perturbed gut
VRE gobbles up enriched sugars and amino acids, and loss of short-chain fatty acids (acetate, propionate, butyrate) eliminates natural growth brakes
Therapeutic angle: Prebiotic SCFA mixtures block VRE growth!
VRE gobbles up enriched sugars and amino acids, and loss of short-chain fatty acids (acetate, propionate, butyrate) eliminates natural growth brakes
Therapeutic angle: Prebiotic SCFA mixtures block VRE growth!
🔬Calling all microbiologists. 🔬 Ever feel like your paper is too small for some journals, but too solid to sit on?
#FEMSMicrobes welcomes well-executed microbiology manuscripts, and they put special attention on promoting ECR authors, eg the webinar below. 👇 @femsjournals.bsky.social @femsmicro.org
#FEMSMicrobes welcomes well-executed microbiology manuscripts, and they put special attention on promoting ECR authors, eg the webinar below. 👇 @femsjournals.bsky.social @femsmicro.org
Secure your spot for the #FEMSMicrobes Webinar today 🚨. You’ll deep dive into emerging breakthroughs in the field, from antimicrobial discovery to protein profiling to gut fungi imaging. Big insights for health, industry, and environment. 🦠
Register now: buff.ly/7nFyEIj
#FEMSWebinar #Microbes
Register now: buff.ly/7nFyEIj
#FEMSWebinar #Microbes
July 3, 2025 at 4:11 AM
🔬Calling all microbiologists. 🔬 Ever feel like your paper is too small for some journals, but too solid to sit on?
#FEMSMicrobes welcomes well-executed microbiology manuscripts, and they put special attention on promoting ECR authors, eg the webinar below. 👇 @femsjournals.bsky.social @femsmicro.org
#FEMSMicrobes welcomes well-executed microbiology manuscripts, and they put special attention on promoting ECR authors, eg the webinar below. 👇 @femsjournals.bsky.social @femsmicro.org
Reposted by Kimberly Kline 🏔
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
June 17, 2025 at 12:20 PM
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Reposted by Kimberly Kline 🏔
FEMS MICRO Milan is buzzing with excitement! 🎉🧫 Over 1,900 participants from 90+ countries are ready for 1,700+ posters & 100+ sessions (debates, expert panels, keynotes). This is Europe's premier microbiology event🏆🔬
Regular registration closes today, at 23:59 CEST: buff.ly/6f7yHDV
Regular registration closes today, at 23:59 CEST: buff.ly/6f7yHDV
June 14, 2025 at 12:01 PM
FEMS MICRO Milan is buzzing with excitement! 🎉🧫 Over 1,900 participants from 90+ countries are ready for 1,700+ posters & 100+ sessions (debates, expert panels, keynotes). This is Europe's premier microbiology event🏆🔬
Regular registration closes today, at 23:59 CEST: buff.ly/6f7yHDV
Regular registration closes today, at 23:59 CEST: buff.ly/6f7yHDV
Reposted by Kimberly Kline 🏔
Our paper in @science.org 👉🏽 www.science.org/doi/10.1126/...
is accompanied by an especially thoughtful perspective by Carey Nadell and Chris Marx 👉🏽
www.science.org/doi/10.1126/...
is accompanied by an especially thoughtful perspective by Carey Nadell and Chris Marx 👉🏽
www.science.org/doi/10.1126/...
June 13, 2025 at 6:11 AM
Our paper in @science.org 👉🏽 www.science.org/doi/10.1126/...
is accompanied by an especially thoughtful perspective by Carey Nadell and Chris Marx 👉🏽
www.science.org/doi/10.1126/...
is accompanied by an especially thoughtful perspective by Carey Nadell and Chris Marx 👉🏽
www.science.org/doi/10.1126/...
Reposted by Kimberly Kline 🏔
Jun #SGBUG with Aaron Tan from the groups of @kimingeneva.bsky.social and @gthibault.bsky.social talking about exciting findings on E. faecalis pathogenesis, and @weeboont.bsky.social from our lab sharing his newest work on phospholipid transporters in E. coli! @scelse.bsky.social Awesome crowd!
June 12, 2025 at 11:07 AM
Jun #SGBUG with Aaron Tan from the groups of @kimingeneva.bsky.social and @gthibault.bsky.social talking about exciting findings on E. faecalis pathogenesis, and @weeboont.bsky.social from our lab sharing his newest work on phospholipid transporters in E. coli! @scelse.bsky.social Awesome crowd!
Reposted by Kimberly Kline 🏔
New tenure track assistant professor position in molecular microbiology in our department @dmf-unil.bsky.social @unil.bsky.social! We are casting a wide net for an experimental molecular microbiologist. Apply here: wwwfbm.unil.ch/releve/appli...
DMF: Tenure Track Assistant Professor towards Associate Professor in Molecular Microbiology - Site des postulations FBM
wwwfbm.unil.ch
June 10, 2025 at 1:53 PM
New tenure track assistant professor position in molecular microbiology in our department @dmf-unil.bsky.social @unil.bsky.social! We are casting a wide net for an experimental molecular microbiologist. Apply here: wwwfbm.unil.ch/releve/appli...