anna brogan
@apbrogan.bsky.social
PhD student into bacteria & coffee. Rudner lab @harvardmed. @penn_state alum.
Pinned
anna brogan
@apbrogan.bsky.social
· Jun 17
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Reposted by anna brogan
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Proteome-wide in silico screening for human protein-protein interactions
Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...
biorxiv.org
November 12, 2025 at 9:26 PM
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Reposted by anna brogan
Identification of genetic interactions in Bacillus subtilis cell division. Congratulations, Byoung-Mo Koo, Carol Gross, and all involved!
@cp-cellsystems.bsky.social #subtiwiki
www.cell.com/cell-systems...
@cp-cellsystems.bsky.social #subtiwiki
www.cell.com/cell-systems...
Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi
Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions
in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks
in envelope biogenesis and ...
www.cell.com
October 4, 2025 at 10:16 AM
Identification of genetic interactions in Bacillus subtilis cell division. Congratulations, Byoung-Mo Koo, Carol Gross, and all involved!
@cp-cellsystems.bsky.social #subtiwiki
www.cell.com/cell-systems...
@cp-cellsystems.bsky.social #subtiwiki
www.cell.com/cell-systems...
Reposted by anna brogan
Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...
Dual transposon sequencing profiles the genetic interaction landscape in bacteria
Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. We report dual transposon sequencing (dual Tn-seq), a platform...
www.science.org
September 25, 2025 at 11:19 PM
Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...
Reposted by anna brogan
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.
www.nature.com
July 29, 2025 at 1:23 PM
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
Reposted by anna brogan
The #SubtiWiki Paper of the month for June 2025 has been selected.
Congratulations, @apbrogan.bsky.social @ @harvardmed.bsky.social
@natmicrobiol.nature.com
subtiwiki.uni-goettingen.de/wiki/index.p...
Congratulations, @apbrogan.bsky.social @ @harvardmed.bsky.social
@natmicrobiol.nature.com
subtiwiki.uni-goettingen.de/wiki/index.p...
June 30, 2025 at 12:50 PM
The #SubtiWiki Paper of the month for June 2025 has been selected.
Congratulations, @apbrogan.bsky.social @ @harvardmed.bsky.social
@natmicrobiol.nature.com
subtiwiki.uni-goettingen.de/wiki/index.p...
Congratulations, @apbrogan.bsky.social @ @harvardmed.bsky.social
@natmicrobiol.nature.com
subtiwiki.uni-goettingen.de/wiki/index.p...
Reposted by anna brogan
🚨Out now!
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis @harvardmicro.bsky.social
#MicroSky 🦠
www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis @harvardmicro.bsky.social
#MicroSky 🦠
www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
June 18, 2025 at 1:51 PM
🚨Out now!
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis @harvardmicro.bsky.social
#MicroSky 🦠
www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis @harvardmicro.bsky.social
#MicroSky 🦠
www.nature.com/articles/s41...
Reposted by anna brogan
Brogan et al uncover a signaling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases the cytoplasmic turgor pressure and protects the cell from lysis: www.nature.com/articles/s41...
June 17, 2025 at 1:01 PM
Brogan et al uncover a signaling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases the cytoplasmic turgor pressure and protects the cell from lysis: www.nature.com/articles/s41...
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
June 17, 2025 at 12:20 PM
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Reposted by anna brogan
I’m excited that the work by Diego Ramirez and Lei Yin is out, where they gained several key insights into what provides the force underlying bacterial cell division doi.org/10.1101/2025....
To divide, cells must first bend the membrane inward, a process that’s energetically expensive
To divide, cells must first bend the membrane inward, a process that’s energetically expensive
The interplay of membrane tension and FtsZ filament condensation on the initiation and progression of cell division in B. subtilis
The first step of cell division is deforming the planar cell membrane inward towards the cytoplasm. As deforming membranes is energetically costly, biology has developed various protein systems to acc...
doi.org
May 20, 2025 at 5:17 PM
I’m excited that the work by Diego Ramirez and Lei Yin is out, where they gained several key insights into what provides the force underlying bacterial cell division doi.org/10.1101/2025....
To divide, cells must first bend the membrane inward, a process that’s energetically expensive
To divide, cells must first bend the membrane inward, a process that’s energetically expensive
Reposted by anna brogan
🚨👉 Please check our recent work on bacterial cell division. In situ Cryo-ET reveals the cellular function of the penicillin binding protein 1b supported by AFM, live-cell imaging, in silico AlphaFold proteome screen and TIRFM. Hope you enjoy the read! #teamtomo #cryo-ET ❄️🔬🐎 big thanks to the team!
The aPBP-type cell wall synthase PBP1b plays a specialized role in fortifying the Escherichia coli division site against osmotic rupture https://www.biorxiv.org/content/10.1101/2025.04.02.646830v1
April 3, 2025 at 9:08 AM
Reposted by anna brogan
Very excited to share that my thesis work is out in Molecular Cell! We trained a Structure and Omics informed Classifier (SPOC) to score binary AlphaFold multimer (AF-M) predictions by structural quality and consistency with experimental omics datasets. www.cell.com/molecular-ce...
Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions
Schmid and Walter train a classifier that discerns functionally relevant structure
predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer,
and they use this co...
www.cell.com
February 26, 2025 at 6:14 PM
Very excited to share that my thesis work is out in Molecular Cell! We trained a Structure and Omics informed Classifier (SPOC) to score binary AlphaFold multimer (AF-M) predictions by structural quality and consistency with experimental omics datasets. www.cell.com/molecular-ce...
Reposted by anna brogan
SAVE THE DATE ‼️ 📆 📢
BBM 2025 will be held on June 9-10th at the Harvard Science Center feat. the one and only Dr. Petra Levin as keynote! We can't wait to see you there.
Registration and scholarship applications open next week.
BBM 2025 will be held on June 9-10th at the Harvard Science Center feat. the one and only Dr. Petra Levin as keynote! We can't wait to see you there.
Registration and scholarship applications open next week.
February 1, 2025 at 3:05 PM
SAVE THE DATE ‼️ 📆 📢
BBM 2025 will be held on June 9-10th at the Harvard Science Center feat. the one and only Dr. Petra Levin as keynote! We can't wait to see you there.
Registration and scholarship applications open next week.
BBM 2025 will be held on June 9-10th at the Harvard Science Center feat. the one and only Dr. Petra Levin as keynote! We can't wait to see you there.
Registration and scholarship applications open next week.
Angelika Gründling’s work from her sabbatical in the Bernhardt-Rudner labs is out. She came with the goal of finding the missing G+ phosphatidylglycerol phosphate phosphatase and found it with time to spare. I can also highly recommend her as a bay mate! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
January 28, 2025 at 3:10 PM
Angelika Gründling’s work from her sabbatical in the Bernhardt-Rudner labs is out. She came with the goal of finding the missing G+ phosphatidylglycerol phosphate phosphatase and found it with time to spare. I can also highly recommend her as a bay mate! www.pnas.org/doi/10.1073/...
Reposted by anna brogan
HARIBO bacter for happy holidays
December 20, 2024 at 3:38 PM
HARIBO bacter for happy holidays