László Tora
@tlaszlo.bsky.social
Transcription regulation, how genes are turned on or off, transcriptional cofactors, general transcription factors and core promoters.
Reposted by László Tora
High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo https://www.biorxiv.org/content/10.64898/2025.12.22.691305v1
December 23, 2025 at 2:32 PM
High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo https://www.biorxiv.org/content/10.64898/2025.12.22.691305v1
Read our new preprint where we uncover a hierarchy in human PIC assembly and establish a quantitative framework that connects factor exchange kinetics to the regulation of Pol II activity in living human cells. doi: doi.org/10.64898/202...
By A. Oravecz and our collaborators @molinalab.bsky.social
By A. Oravecz and our collaborators @molinalab.bsky.social
RNA polymerase II initiation factors show different dynamic behaviour upon induced transcription in live cells
Transcription by RNA polymerase II (Pol II) requires the ordered action of general transcription factors (GTFs) forming the pre-initiation complex (PIC). How these events unfold kinetically remains un...
doi.org
December 24, 2025 at 1:35 PM
Read our new preprint where we uncover a hierarchy in human PIC assembly and establish a quantitative framework that connects factor exchange kinetics to the regulation of Pol II activity in living human cells. doi: doi.org/10.64898/202...
By A. Oravecz and our collaborators @molinalab.bsky.social
By A. Oravecz and our collaborators @molinalab.bsky.social
Reposted by László Tora
Intrigued by a long-standing conundrum in small RNA biology—how nuclear Argonaute proteins silence transposons when they *need* target transcription for their own recruitment—we studied the piRNA pathway.
And found a hidden RNA-decay axis from Piwi to the RNA exosome.
And found a hidden RNA-decay axis from Piwi to the RNA exosome.
RNA decay via the nuclear exosome is essential for piwi-mediated transposon silencing https://www.biorxiv.org/content/10.64898/2025.12.16.694471v1
December 22, 2025 at 6:14 PM
Intrigued by a long-standing conundrum in small RNA biology—how nuclear Argonaute proteins silence transposons when they *need* target transcription for their own recruitment—we studied the piRNA pathway.
And found a hidden RNA-decay axis from Piwi to the RNA exosome.
And found a hidden RNA-decay axis from Piwi to the RNA exosome.
Reposted by László Tora
All of this was spearheaded by the exceptional experimentalist & scientist @changweiyu.bsky.social who came with a strong background in transcription from @tlaszlo.bsky.social and developed into a remarkable RNA biologist.
December 22, 2025 at 6:14 PM
All of this was spearheaded by the exceptional experimentalist & scientist @changweiyu.bsky.social who came with a strong background in transcription from @tlaszlo.bsky.social and developed into a remarkable RNA biologist.
Reposted by László Tora
Please RT 📧
Registration open for ➡️"Summer School on Chromatin Biology" Our 2 weeks hands on expedition 🧪👩💻August 2026. 3. edition. Learn CUT@Tag, CUT@Run, ChIPseq, ATACseq AND to analyse your own data at @helmholtzmunich.bsky.social Daily letures by experts in the field !
▶️ shorturl.at/jrA8i
Registration open for ➡️"Summer School on Chromatin Biology" Our 2 weeks hands on expedition 🧪👩💻August 2026. 3. edition. Learn CUT@Tag, CUT@Run, ChIPseq, ATACseq AND to analyse your own data at @helmholtzmunich.bsky.social Daily letures by experts in the field !
▶️ shorturl.at/jrA8i
December 9, 2025 at 11:59 AM
Please RT 📧
Registration open for ➡️"Summer School on Chromatin Biology" Our 2 weeks hands on expedition 🧪👩💻August 2026. 3. edition. Learn CUT@Tag, CUT@Run, ChIPseq, ATACseq AND to analyse your own data at @helmholtzmunich.bsky.social Daily letures by experts in the field !
▶️ shorturl.at/jrA8i
Registration open for ➡️"Summer School on Chromatin Biology" Our 2 weeks hands on expedition 🧪👩💻August 2026. 3. edition. Learn CUT@Tag, CUT@Run, ChIPseq, ATACseq AND to analyse your own data at @helmholtzmunich.bsky.social Daily letures by experts in the field !
▶️ shorturl.at/jrA8i
Reposted by László Tora
Structure of the transcriptional co-activator SAGA complex (from fungus Chaetomium thermophilum), including the histone acetyltransferase module #cryoEM
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
November 21, 2025 at 11:00 AM
Structure of the transcriptional co-activator SAGA complex (from fungus Chaetomium thermophilum), including the histone acetyltransferase module #cryoEM
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Reposted by László Tora
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Reposted by László Tora
Today it was announced that Sir John Gurdon passed away.
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
Nobel Laureate Professor Sir John Gurdon dies aged 92
It is with great sadness that the University shares the news of the death of Professor Sir John Gurdon, founder of the Gurdon Institute.
www.cam.ac.uk
October 8, 2025 at 11:56 AM
Today it was announced that Sir John Gurdon passed away.
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
Reposted by László Tora
With self-deprecating humor, Sir John loved to tell the story of his Biology teacher (1949)
October 8, 2025 at 12:10 PM
With self-deprecating humor, Sir John loved to tell the story of his Biology teacher (1949)
Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...
FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics
The FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics will take place 19–22 October 2026 in Budapest, Hungary. Here we give a taste of the scientific content, flag some of the speaker...
network.febs.org
September 10, 2025 at 2:24 PM
Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...
Reposted by László Tora
Reposted by László Tora
"In our view, it [hexanediol] should thus be avoided and not be used as evidence for the formation of an assembly via phase separation or the fluid-like nature of a condensate in vitro or in vivo." doi.org/10.1038/s414...
Current practices in the study of biomolecular condensates: a community comment - Nature Communications
The realization that the cell is abundantly compartmentalized into biomolecular condensates has opened new opportunities for understanding the physics and chemistry underlying many cellular processes1...
doi.org
August 21, 2025 at 3:38 PM
"In our view, it [hexanediol] should thus be avoided and not be used as evidence for the formation of an assembly via phase separation or the fluid-like nature of a condensate in vitro or in vivo." doi.org/10.1038/s414...
Read this interesting preprint from the lab of Krishnamurthy Natarajan: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
doi: doi.org/10.1101/2025...
doi: doi.org/10.1101/2025...
Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
The fidelity of assembly of multiprotein complexes is essential for the formation of stable and functional protein complexes that are critical for cell growth and survival. In this context, TBP-associ...
doi.org
August 29, 2025 at 3:24 PM
Read this interesting preprint from the lab of Krishnamurthy Natarajan: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
doi: doi.org/10.1101/2025...
doi: doi.org/10.1101/2025...
Reposted by László Tora
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
August 29, 2025 at 12:36 AM
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Check out our new collaborative preprint about Affinity-ligand purification of native human low-abundance multi-protein complexes doi.org/10.1101/2025...
Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination
In human cells, large multi-protein transcription co-activators, such as chromatin remodelers or histone acetyltransferases, play critical roles in gene-expression regulation and are often implicated ...
doi.org
August 12, 2025 at 7:30 AM
Check out our new collaborative preprint about Affinity-ligand purification of native human low-abundance multi-protein complexes doi.org/10.1101/2025...
Reposted by László Tora
Exquisite work. Well done to the whole team.
How to find Evolutionary Conserved Enhancers in 2025? 🐣-🐭
Check out our paper - fresh off the press!!!
We find widespread functional conservation of enhancers in absence of sequence homology
Including: a bioinformatic tool to map sequence-diverged enhancers!
rdcu.be/enVDN
github.com/tobiaszehnde...
Check out our paper - fresh off the press!!!
We find widespread functional conservation of enhancers in absence of sequence homology
Including: a bioinformatic tool to map sequence-diverged enhancers!
rdcu.be/enVDN
github.com/tobiaszehnde...
Conservation of regulatory elements with highly diverged sequences across large evolutionary distances
Nature Genetics - Combining functional genomic data from mouse and chicken with a synteny-based strategy identifies positionally conserved cis-regulatory elements in the absence of direct sequence...
rdcu.be
May 28, 2025 at 5:33 AM
Exquisite work. Well done to the whole team.
Reposted by László Tora
🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...
May 17, 2025 at 5:00 PM
🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...
Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !
May 1, 2025 at 7:08 AM
Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !
Reposted by László Tora
The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...
April 2, 2025 at 8:47 AM
The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...
Reposted by László Tora
Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!
March 26, 2025 at 12:17 PM
Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!
Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
March 21, 2025 at 5:27 PM
Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
Reposted by László Tora
Reposted by László Tora
Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz
woodstock.img.cas.cz
March 1, 2025 at 5:44 PM
Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz
woodstock.img.cas.cz
Reposted by László Tora
Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...
Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma
The midnolin-proteasome pathway degrades many nuclear proteins without ubiquitination, but how it operates mechanistically remains unclear. Here, we present structures of the midnolin-proteasome compl...
www.biorxiv.org
February 24, 2025 at 2:11 AM
Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...
Reposted by László Tora
‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
February 23, 2025 at 10:13 PM
‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...