Mariusz Czarnocki-Cieciura
@mariuszczc.bsky.social
Structural biologist in the Nowotny lab at IIMCB Warsaw, Poland #cryoEM
Reposted by Mariusz Czarnocki-Cieciura
I am excited to share our new preprint on our structural and biochemical analyses of human methionine synthase (MTR). This was the hard work of Douglas Ferreira who carried out this research as a significant part of his PhD. #CryoEM www.biorxiv.org/content/10.1...
Structural insights into cobalamin loading and reactivation of human methionine synthase
Human methionine synthase (MTR) is an essential enzyme of one carbon metabolism. Consisting of a catalytic N-half and a cobalamin binding C-half, MTR utilises this intricate organometallic cofactor in...
www.biorxiv.org
November 11, 2025 at 12:48 PM
I am excited to share our new preprint on our structural and biochemical analyses of human methionine synthase (MTR). This was the hard work of Douglas Ferreira who carried out this research as a significant part of his PhD. #CryoEM www.biorxiv.org/content/10.1...
Reposted by Mariusz Czarnocki-Cieciura
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
August 30, 2025 at 2:11 PM
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
Reposted by Mariusz Czarnocki-Cieciura
🧬 Transcription elongation by RNA polymerase II relies on a web of elongation factors. Our new work shows how IWS1 acts as a modular scaffold to stabilize & stimulate elongation. Fantastic work by Della Syau! www.biorxiv.org/content/10.1...
August 29, 2025 at 10:14 AM
🧬 Transcription elongation by RNA polymerase II relies on a web of elongation factors. Our new work shows how IWS1 acts as a modular scaffold to stabilize & stimulate elongation. Fantastic work by Della Syau! www.biorxiv.org/content/10.1...
Reposted by Mariusz Czarnocki-Cieciura
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
biorxiv.org
July 23, 2025 at 9:43 PM
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
Reposted by Mariusz Czarnocki-Cieciura
Is anyone using a nitrogen gas generator instead of a high-pressure liquid cylinder to feed a cryo-FIB heat exchanger? Just wondering - seems like commercial generators (e.g. for LC-MS) can make ~3-5x the needed flow with a slightly smaller footprint #TeamTomo
July 2, 2025 at 11:38 PM
Is anyone using a nitrogen gas generator instead of a high-pressure liquid cylinder to feed a cryo-FIB heat exchanger? Just wondering - seems like commercial generators (e.g. for LC-MS) can make ~3-5x the needed flow with a slightly smaller footprint #TeamTomo
Reposted by Mariusz Czarnocki-Cieciura
Structure from Noise: Confirmation Bias in Particle Picking in Structural Biology [new]
Cryo-EM/ET picking bias creates artificial "structure from noise" w/ template matching/NNs. Mitigation strategies exist.
Cryo-EM/ET picking bias creates artificial "structure from noise" w/ template matching/NNs. Mitigation strategies exist.
July 6, 2025 at 6:26 AM
Structure from Noise: Confirmation Bias in Particle Picking in Structural Biology [new]
Cryo-EM/ET picking bias creates artificial "structure from noise" w/ template matching/NNs. Mitigation strategies exist.
Cryo-EM/ET picking bias creates artificial "structure from noise" w/ template matching/NNs. Mitigation strategies exist.
Reposted by Mariusz Czarnocki-Cieciura
I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.
biorxiv.org/content/earl...
biorxiv.org/content/earl...
June 26, 2025 at 3:34 PM
I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.
biorxiv.org/content/earl...
biorxiv.org/content/earl...
Reposted by Mariusz Czarnocki-Cieciura
Structural insights into DNA annealing and recombination by herpesviral DNA-binding proteins ICP8 and BALF2 https://www.biorxiv.org/content/10.1101/2025.06.25.661518v1
June 26, 2025 at 3:20 AM
Structural insights into DNA annealing and recombination by herpesviral DNA-binding proteins ICP8 and BALF2 https://www.biorxiv.org/content/10.1101/2025.06.25.661518v1
Reposted by Mariusz Czarnocki-Cieciura
New @ncn.gov.pl OPUS 28 grant for Prof. Marcin Nowotny! 🧬
The project will explore how adenoviruses replicate their DNA - a poorly understood process using cryo-EM and crystallography. Total funding: PLN 3,174,698 🏆
The project will explore how adenoviruses replicate their DNA - a poorly understood process using cryo-EM and crystallography. Total funding: PLN 3,174,698 🏆
May 30, 2025 at 3:47 PM
New @ncn.gov.pl OPUS 28 grant for Prof. Marcin Nowotny! 🧬
The project will explore how adenoviruses replicate their DNA - a poorly understood process using cryo-EM and crystallography. Total funding: PLN 3,174,698 🏆
The project will explore how adenoviruses replicate their DNA - a poorly understood process using cryo-EM and crystallography. Total funding: PLN 3,174,698 🏆
Reposted by Mariusz Czarnocki-Cieciura
We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation.
Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.
cryotud.github.io/locscale/
Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.
cryotud.github.io/locscale/
May 22, 2025 at 10:10 AM
We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation.
Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.
cryotud.github.io/locscale/
Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.
cryotud.github.io/locscale/
Reposted by Mariusz Czarnocki-Cieciura
Out now: Another example of how lipidomics led us to see the full(er) picture - enigmatic membrane protein OCIAD1 has a functional role in peroxisomes. Great team work with students and collaborators alike 🧫👩🔬🔬
Following lipidomic and proteomic analyses, Vanessa Linke @massspecvanili.bsky.social Agnieszka Chacinska and colleagues identify a role for OCIAD1 at the mitochondria-peroxisome intersection balancing lipid metabolism.
journals.biologists.com/jcs/article/...
#JCSMitoSI #Mitochondria
journals.biologists.com/jcs/article/...
#JCSMitoSI #Mitochondria
May 20, 2025 at 7:36 AM
Out now: Another example of how lipidomics led us to see the full(er) picture - enigmatic membrane protein OCIAD1 has a functional role in peroxisomes. Great team work with students and collaborators alike 🧫👩🔬🔬
Reposted by Mariusz Czarnocki-Cieciura
We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵
Determining the effects of pseudouridine incorporation on human tRNAs | The EMBO Journal
imageimagetRNAs harbour a complex set of modifications which are important for their function.
Here, single particle cryo-EM, biophysics, and computational analyses of the effects
of pseudouridine inc...
www.embopress.org
April 30, 2025 at 4:12 AM
We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵
Reposted by Mariusz Czarnocki-Cieciura
I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY
Molecular basis of human GABA transporter 3 inhibition
Nature Communications - GABA transporters maintain GABA homeostasis and regulate synaptic signaling. Here, the authors determined the cryoEM structures of GABA transporter 3 (GAT3) in its apo form...
urldefense.com
April 23, 2025 at 9:37 PM
I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY
Reposted by Mariusz Czarnocki-Cieciura
Super excited to share this as my first post with the 🦋 world! In my first co-correspondent article, we (@Mayra Borrero @Agnieszka Chacińska @IMOL Institute) look at the roles of lipids in mitochondrial protein import complexes (spoiler: there are plenty!) &call for methods (spoiler: there are few!)
Available online - the Forum "#Lipids: emerging actors in mitochondrial protein import" from Vanessa Linke, Agnieszka Chacinska, et al.
#ProteinLipidInteractions #MembraneComplexes #LipidScrambling
Read it here: authors.elsevier.com/a/1kxPM_M0e4...
#ProteinLipidInteractions #MembraneComplexes #LipidScrambling
Read it here: authors.elsevier.com/a/1kxPM_M0e4...
April 18, 2025 at 6:21 PM
Super excited to share this as my first post with the 🦋 world! In my first co-correspondent article, we (@Mayra Borrero @Agnieszka Chacińska @IMOL Institute) look at the roles of lipids in mitochondrial protein import complexes (spoiler: there are plenty!) &call for methods (spoiler: there are few!)
Reposted by Mariusz Czarnocki-Cieciura
🎉 Incredibly proud to share my first-author paper in
@nature.com! Heartfelt thanks to my mentor @andrzejdziembowski.bsky.social and the brilliant team at DziembowskiLab at @iimcb.bsky.social. More details in the 🧵below ⬇️
@nature.com! Heartfelt thanks to my mentor @andrzejdziembowski.bsky.social and the brilliant team at DziembowskiLab at @iimcb.bsky.social. More details in the 🧵below ⬇️
April 17, 2025 at 12:15 PM
🎉 Incredibly proud to share my first-author paper in
@nature.com! Heartfelt thanks to my mentor @andrzejdziembowski.bsky.social and the brilliant team at DziembowskiLab at @iimcb.bsky.social. More details in the 🧵below ⬇️
@nature.com! Heartfelt thanks to my mentor @andrzejdziembowski.bsky.social and the brilliant team at DziembowskiLab at @iimcb.bsky.social. More details in the 🧵below ⬇️
Reposted by Mariusz Czarnocki-Cieciura
🌐Our @nature.com paper is out! Researchers from IIMCB and other Polish institutions show how TENT5A boosts mRNA vaccine efficacy by stabilizing therapeutic mRNAs and enhancing antigen production. A step toward better therapies for cancer and beyond! 🧬
👉Read the story: bit.ly/3XYvQkI
#Nature #mRNA
👉Read the story: bit.ly/3XYvQkI
#Nature #mRNA
IIMCB
bit.ly
April 16, 2025 at 6:00 PM
🌐Our @nature.com paper is out! Researchers from IIMCB and other Polish institutions show how TENT5A boosts mRNA vaccine efficacy by stabilizing therapeutic mRNAs and enhancing antigen production. A step toward better therapies for cancer and beyond! 🧬
👉Read the story: bit.ly/3XYvQkI
#Nature #mRNA
👉Read the story: bit.ly/3XYvQkI
#Nature #mRNA
Reposted by Mariusz Czarnocki-Cieciura
👋 Hello BlueSky! We’re the International Institute of Molecular and Cell Biology in Warsaw – a research institute at the forefront of life sciences. Our scientists explore the molecular and cellular mechanisms of life, combining curiosity-driven research with scientific and societal relevance.
April 15, 2025 at 1:43 PM
👋 Hello BlueSky! We’re the International Institute of Molecular and Cell Biology in Warsaw – a research institute at the forefront of life sciences. Our scientists explore the molecular and cellular mechanisms of life, combining curiosity-driven research with scientific and societal relevance.
I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex.
www.jbc.org/article/S002...
www.jbc.org/article/S002...
April 15, 2025 at 10:07 AM
I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex.
www.jbc.org/article/S002...
www.jbc.org/article/S002...
Reposted by Mariusz Czarnocki-Cieciura
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
April 6, 2025 at 9:45 AM
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
Reposted by Mariusz Czarnocki-Cieciura
Hello BlueSky! Inaugural post here from the Sternberg Lab. We're excited to share our latest work, in which we teamed up with the @WiedenheftLab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: www.biorxiv.org/content/10.1...
Protein-primed DNA homopolymer synthesis by an antiviral reverse transcriptase
Bacteria defend themselves from viral predation using diverse immune systems, many of which sense and target foreign DNA for degradation. Defense-associated reverse transcriptase (DRT) systems provide...
www.biorxiv.org
March 26, 2025 at 10:13 PM
Hello BlueSky! Inaugural post here from the Sternberg Lab. We're excited to share our latest work, in which we teamed up with the @WiedenheftLab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: www.biorxiv.org/content/10.1...
Reposted by Mariusz Czarnocki-Cieciura
I am super excited to share our work uncovering the structure and mechanism of an ATPase-associated reverse transcriptase (RT) involved in prokaryotic antiviral defense, now available as a preprint on bioRxiv!🧬🦠🔬
www.biorxiv.org/content/10.1...
🧵👇
www.biorxiv.org/content/10.1...
🧵👇
Structural basis of antiphage defense by an ATPase-associated reverse transcriptase
Reverse transcriptases (RTs) have well-established roles in the replication and spread of retroviruses and retrotransposons. However, recent evidence suggests that RTs have been conscripted by cells f...
www.biorxiv.org
March 27, 2025 at 1:59 PM
I am super excited to share our work uncovering the structure and mechanism of an ATPase-associated reverse transcriptase (RT) involved in prokaryotic antiviral defense, now available as a preprint on bioRxiv!🧬🦠🔬
www.biorxiv.org/content/10.1...
🧵👇
www.biorxiv.org/content/10.1...
🧵👇
Reposted by Mariusz Czarnocki-Cieciura
🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
March 28, 2025 at 3:44 PM
🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
Reposted by Mariusz Czarnocki-Cieciura
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:20 PM
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
Fascinating things happening at the IIMCB nowadays! This is one of the first patterns milled with our new TFS Hydra Bio Plasma-FIB, and - most likely - the smallest IIMCB logo ever!
March 13, 2025 at 11:21 AM
Fascinating things happening at the IIMCB nowadays! This is one of the first patterns milled with our new TFS Hydra Bio Plasma-FIB, and - most likely - the smallest IIMCB logo ever!
Reposted by Mariusz Czarnocki-Cieciura
Did you know that two jumping sequences (LINE-1 and Alu) make up nearly 30% of our genomes? Ever wonder how they jump into new genomic sites? We have some exciting discoveries to share new in @science.org on the structural mechanisms of LINE-1 retrotransposition (aka jumping 🧬➡️🧬) 🧪 #ScienceResearch
March 7, 2025 at 12:22 AM
Did you know that two jumping sequences (LINE-1 and Alu) make up nearly 30% of our genomes? Ever wonder how they jump into new genomic sites? We have some exciting discoveries to share new in @science.org on the structural mechanisms of LINE-1 retrotransposition (aka jumping 🧬➡️🧬) 🧪 #ScienceResearch