AlphaFold 3 predictions suggest that the tRNA interacts only with a small region of the RtcB protein, which would make it quite mobile. Interestingly, the same region is also predicted to be involved in DDX1 binding.

I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex.
www.jbc.org/article/S002...