Stanley Michi Kanai (He/Him)
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kanaimichi.bsky.social
Stanley Michi Kanai (He/Him)
@kanaimichi.bsky.social
57 followers 70 following 4 posts
I study GPCR and G protein signaling in craniofacial development. #zebrafish

Research Associate at University of Colorado Anschutz Medical Campus.
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kanaimichi.bsky.social
Stanley Michi Kanai (He/Him) @kanaimichi.bsky.social · Apr 23
Our paper is out! We address a long-debated question regarding the role of Gα proteins in lower jaw patterning.
dev-journal.bsky.social Development @dev-journal.bsky.social · Apr 21
The Gq/11 family of Gα subunits is necessary and sufficient for lower jaw development

Read this #OpenAccess Research Article by Stanley Kanai, David Clouthier and colleagues:
journals.biologists.com/dev/article/...
Figure 2 YM causes defects to a subset of Edn1/Ednra-dependent lower jaw skeletal elements. (A-D) Lateral views of 6 dpf embryos treated with DMSO (A,C) or YM (B,D), shown in gross (A,B) or whole-mount skeletal preparations of larvae (C,D). The asterisk in B indicates the absence of an inflated swim bladder (sb). (E-J) Ventral (E-G) or lateral (H-J) views of flat-mounts for the viscerocranium from 6 dpf wild-type larvae treated with DMSO (E,H) or YM (F,I), or an untreated edn1−/− larvae (G,J). In E,F,H,I, white outlines highlight the symplectic cartilage (sy). In F,I, black arrowheads indicate fusion of the jaw joint (jj), white arrowhead indicates fusion of the hyomandibular joint (hj) and the arrow indicates fusion of the opercle (op) and branchiostegal ray (bsr). In G,J, the white asterisk indicates absence of the ceratohyal (ch).
Reposted by Stanley Michi Kanai (He/Him)
emkolen.bsky.social
Excited to share that our work building in vivo biosensors for receptor tyrosine kinases (pYtags!) is now out www.cell.com/cell-reports...
July 1, 2025 at 1:58 PM
Reposted by Stanley Michi Kanai (He/Him)
emkolen.bsky.social
We built pYtags to detect RTK activity in Drosophila embryos and used them to learn about how developmental patterns form bsky.app/profile/emko...
emkolen.bsky.social Emily Kolenbrander Ho @emkolen.bsky.social · Jan 7
Have you ever wanted to *see* receptor activity in embryos? If so, our new preprint is for you! In it, we showcase a new live-cell biosensor for visualizing receptor tyrosine kinase activity in living embryos – pYtags!

www.biorxiv.org/content/10.1...
July 1, 2025 at 1:58 PM
Reposted by Stanley Michi Kanai (He/Him)
biologists.bsky.social
Following on from their successful meeting in 2023,
@jcellsci.bsky.social are delighted to announce a second iteration of the Imaging Cell Dynamics Meeting to be held in 2026.

Find out more:

www.biologists.com/meetings/jcs...

#Cells #CellDyamics #CellScience #Imaging #JCS #Microscopy
Journal of Cell Science Meeting Imaging Cell Dynamics 11-14 May 2026 Montanyà Hotel, Catalonia, Spain Register your interest
June 27, 2025 at 1:46 PM
Reposted by Stanley Michi Kanai (He/Him)
joann-trejo.bsky.social
Our latest work is out "ARRDC3 tyrosine phosphorylation functions as a switch to control c-Src versus WWP2 interactions and distinct scaffolding functions" led by grad student Mika Caplan @ucsandiego.bsky.social @asbmb.bsky.social, Open access, J. Biol. Chem doi.org/10.1016/j.jb...
June 28, 2025 at 9:47 PM
Reposted by Stanley Michi Kanai (He/Him)
zebrafishrock.bsky.social
A parting gift to stay connected ❤️ 🧪

Starter Packs for #zebrafish Community:

ZF Resources: go.bsky.app/JcC5df3
#WomenInScience V1: go.bsky.app/4BegEjv
#WomenInScience V2: go.bsky.app/VoebYrt
ZF PIs V1: go.bsky.app/2cHYybY
ZF PIs V2: go.bsky.app/4zBuxAF
ZF PIs V3: go.bsky.app/JvjuSZg
June 29, 2025 at 6:42 AM
Reposted by Stanley Michi Kanai (He/Him)
devbiol.bsky.social
#DBfeature

Development of ectodermal and endodermal taste buds

by Linda Barlow @bizzarlow.bsky.social
www.sciencedirect.com/science/arti...
June 27, 2025 at 2:56 PM
Reposted by Stanley Michi Kanai (He/Him)
lablerit.bsky.social
Our lab @emoryuniversity.bsky.social Dept. of Cell Biology is hiring a research technician! This position is ideal for someone who enjoys research, organization, and working in a team. Come for a few years and publish with us, or stay to build a career. staff-emory.icims.com/jobs/149068/...
In this light microscopy image of dividing cells within a Drosophila embryo, multiple centrosomes are labeled in cyan. Each centrosome is surrounded by a concentration of orange fluorescent dots, showing the localization of a model mRNA via single molecule detection.
June 26, 2025 at 6:30 PM
Reposted by Stanley Michi Kanai (He/Him)
joachimgoedhart.bsky.social
De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...
Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.
June 26, 2025 at 1:50 PM
Reposted by Stanley Michi Kanai (He/Him)
nature.com
Nature research paper: Spatiotemporal orchestration of mitosis by cyclin-dependent kinase

https://go.nature.com/444lRhe
Spatiotemporal orchestration of mitosis by cyclin-dependent kinase - Nature
Experiments in fission yeast show that cyclin-dependent kinase is first activated in the nucleus, from which the mitotic signal is propagated from CDK at the centrosome to the cytoplasm.
go.nature.com
June 25, 2025 at 5:50 PM
Reposted by Stanley Michi Kanai (He/Him)
zebrafishrock.bsky.social
ICYMI from @scekker.bsky.social et al.
June 24, 2025 at 4:32 PM
Reposted by Stanley Michi Kanai (He/Him)
evgenykvon.bsky.social
How do non-coding variants in enhancers lead to disease? Happy to share our recent work, led by @ewholling.bsky.social, in which we discovered that poised chromatin sensitizes enhancers to aberrant activation by non-coding mutations, contributing to disease. www.biorxiv.org/content/10.1... 1/
June 23, 2025 at 12:57 PM
Reposted by Stanley Michi Kanai (He/Him)
joachimgoedhart.bsky.social
Cell barcoding with tandem fluorescent proteins enables high-throughput signaling dynamics analysis by Chuan-Hsiang Huang and team: www.biorxiv.org/content/10.1...
Multiplexed tracking of biosensor activities using sctFP barcodes
June 20, 2025 at 12:41 PM
Reposted by Stanley Michi Kanai (He/Him)
neuroseq.bsky.social
I feel incredibly honored and grateful to receive the SDB Elizabeth D. Hay New Investigator Award. Thank you @socdevbio.bsky.social for this recognition! I am looking forward to connecting with everyone at #ICDB2025 and sharing our work and excitement about #DevBio. See you there!
socdevbio.bsky.social Society for Developmental Biology @socdevbio.bsky.social · Jun 16
Mubarak Hussain Syed @neuroseq.bsky.social receives the SDB Elizabeth D. Hay New Investigator Award for their work on understanding the transcriptional rules during development of the fly CNS #ICDB2025 🪰🧪
An announcement slide in white and blue colors, with Mubarak H's name, the SDB ISDB and LASDB logos and text that says Award Lectures II: SDB Elizabeth D Hay New Investigator Award. Sunday, June 22nd 4:20-6pm.
June 16, 2025 at 10:18 AM
Reposted by Stanley Michi Kanai (He/Him)
mikepiacentino.bsky.social
We're #hiring! Our group in the Cell Bio Dept at Hopkins is looking for a Research Technologist to join us and study #neuralcrest #cellmigration during #devbiol! Looking to build up your lab experience? Check it out! @socdevbio.bsky.social @ascbiology.bsky.social
jobs.jhu.edu/job/Baltimor...
Research Technologist
Research Technologist
jobs.jhu.edu
June 14, 2025 at 1:33 PM
Reposted by Stanley Michi Kanai (He/Him)
focalplane.bsky.social
🔬Our preprint list is now up on FocalPlane.
This week we focus on new tools and techniques in imaging. Let us know if we are missing any recent preprints that you've enjoyed reading.
focalplane.biologists.com/2025/06/13/m...
Microscopy preprints – new tools and techniques in imaging - FocalPlane
Microscopy preprints – new tools and techniques in imaging - News
focalplane.biologists.com
June 13, 2025 at 2:40 PM
Reposted by Stanley Michi Kanai (He/Him)
jeffreyafarrell.bsky.social
If you love Daniocell, but wish it could generate analyses specific to YOUR genes & cell types of interest, check out DaniocellDesktop - a new point-and-click app for Mac and Windows that enables reanalysis of the Daniocell data without programming: daniocell.nichd.nih.gov/desktop/ (1/4)
DaniocellDesktop logo, depicting a fish on a laptop screen.
June 13, 2025 at 2:30 PM
Reposted by Stanley Michi Kanai (He/Him)
devbiol.bsky.social
#DBfeature

Pax2a, Pax5 and Cdh1-β-catenin, but not Wnt, protect sensory hair cells from destabilizing effects of fgf signaling on cell adhesion

by Whitney Roberson, Jorden Holland, Bruce Riley
www.sciencedirect.com/science/arti...
June 13, 2025 at 8:04 PM
Reposted by Stanley Michi Kanai (He/Him)
nicolettapetridou.bsky.social
Here our latest work on how morphogens trigger tissue phase transitions to tune their own length-scales and time-scales - An exciting collaboration with @zhvas.bsky.social @dianakhorom.bsky.social Bernat Corominas-Murtra and heroic experiments by the incredible @cami-autorino.bsky.social @embl.org
cami-autorino.bsky.social Camilla Autorino @cami-autorino.bsky.social · Jun 11
🔔 Proud to share the preprint of my PhD work in the Petridou group @nicolettapetridou.bsky.social @embl.org ✨

“A closed feedback between tissue phase transitions and morphogen gradients drives patterning dynamics” 🐟 🔁 📶

🔗 www.biorxiv.org/content/10.1...

#devbio #biophysics

🧵⤵️
June 11, 2025 at 5:24 PM
Reposted by Stanley Michi Kanai (He/Him)
wyomingwormboy.bsky.social
Using classical and molecular genetics along with various tools of cell biology, we show how reduction in a specific membrane lipid species impacts intracellular trafficking but also corrects a compartmental defect resulting from the depletion of a NimA family kinase.
June 12, 2025 at 3:32 AM
Reposted by Stanley Michi Kanai (He/Him)
devbiol.bsky.social
Why Publish in Developmental Biology?
7/8

Reason #7 - ALL DB papers are open access for all after 1 yr.

DB is the official journal of the SDB @SocDevBio

Learn more here
elsevier.com/locate/ydbio
Developmental Biology, the official journal of the SDB, publishes original research on mechanisms of development, differentiation, growth, homeostasis and regeneration in animals and plants at the molecular, cellular, genetic and evolutionary levels.
April 14, 2025 at 11:47 AM
Reposted by Stanley Michi Kanai (He/Him)
evodevopanam.bsky.social
🐟 I’ve been herring all about it!
Fish satellite symposia speaker talks at #PASEDB

Invited speaker: @fishevodevogeno.bsky.social

Organizers: @nakamuralab.bsky.social @patyschneider22.bsky.social & Joaquín Letelier

Register: evodevo.wildapricot.org/event-6007396
June 6, 2025 at 3:03 PM
Reposted by Stanley Michi Kanai (He/Him)
itsthewoolab.bsky.social
Website and registration will open soon for the West Coast SDB meeting, Aug 14–17 at Cal-Poly SLO! Abstract deadline for talks is July 7. Final deadline for abstracts (posters) and registration is July 31. We will have prizes for best undergrad, grad, and post doc presentations! #wcsdb2025 1/4
May 29, 2025 at 11:19 PM
Reposted by Stanley Michi Kanai (He/Him)
vprincelab.bsky.social
Delighted to share our latest work on the anterior lateral line. Congratulations to our fabulous team! www.sciencedirect.com/science/arti...
Development of the zebrafish anterior lateral line system is influenced by underlying cranial neural crest
The mechanosensory lateral line system of aquatic vertebrates comprises a superficial network of distributed sensory organs, the neuromasts, which are…
www.sciencedirect.com
June 4, 2025 at 1:49 AM
kanaimichi.bsky.social
Beautiful!
zebrafishrock.bsky.social Zebrafish Rock! @zebrafishrock.bsky.social · Jun 1
Development of a transgenic zebrafish embryo. Credit to Dr. Mario Mendieta-Serrano. #ZebrafishZunday 🧪
June 1, 2025 at 10:00 PM
Reposted by Stanley Michi Kanai (He/Him)
devbiol.bsky.social
#DBfeature 🦎🦷

Resilience of the replacing dentition in adult reptiles

by Joaquin Henriquez, Joy Richman
www.sciencedirect.com/science/arti...
Pulse-chase labeling of putative tissue resident dental epithelial stem cells in various reptilian dentitions
April 28, 2025 at 12:37 PM