Heilemann Lab
@heilemannlab.bsky.social
Single-molecule biophysics group at Goethe University Frankfurt/Germany. Super-resolution microscopy. Optical structural cell biology. Computational microscopy. Posts by MH.
http://www.smb.uni-frankfurt.de
#superresolution, #livecellimaging, #imageanalysis
http://www.smb.uni-frankfurt.de
#superresolution, #livecellimaging, #imageanalysis
Pinned
Heilemann Lab
@heilemannlab.bsky.social
· Dec 13
#FluorescenceFriday (we are watching vimentin (HaloTag7) in U2OS cells co-stained with Nile Red) #livecell #longterm
Receptor tyrosine kinases bind ligands and (often) dimerize. Yet how long, and how does that change their mobility? Using single-molecule FRET in living cells, we found that MET dimers 'socialize' for ~1 second while lowering lateral diffusion and confinement 👉🏻 doi.org/10.1002/smll...
December 20, 2025 at 11:01 AM
Receptor tyrosine kinases bind ligands and (often) dimerize. Yet how long, and how does that change their mobility? Using single-molecule FRET in living cells, we found that MET dimers 'socialize' for ~1 second while lowering lateral diffusion and confinement 👉🏻 doi.org/10.1002/smll...
Reposted by Heilemann Lab
⏰Call for Applications - Join Our Team and Help Shape Our Cluster
@goetheuni.bsky.social and the Cluster of Excellence SCALE - Subcellular Architecture of Life are seeking 3 highly motivated scientists to join a vibrant and innovative research community on Campus Riedberg.
Share with your network!
@goetheuni.bsky.social and the Cluster of Excellence SCALE - Subcellular Architecture of Life are seeking 3 highly motivated scientists to join a vibrant and innovative research community on Campus Riedberg.
Share with your network!
December 19, 2025 at 10:09 PM
⏰Call for Applications - Join Our Team and Help Shape Our Cluster
@goetheuni.bsky.social and the Cluster of Excellence SCALE - Subcellular Architecture of Life are seeking 3 highly motivated scientists to join a vibrant and innovative research community on Campus Riedberg.
Share with your network!
@goetheuni.bsky.social and the Cluster of Excellence SCALE - Subcellular Architecture of Life are seeking 3 highly motivated scientists to join a vibrant and innovative research community on Campus Riedberg.
Share with your network!
Reposted by Heilemann Lab
My "News & views" was published today @natmethods.nature.com
Thanks @ritastrack.bsky.social for the invitation to write it and Allison Doerr for the editing after Rita left...
www.nature.com/articles/s41...
Thanks @ritastrack.bsky.social for the invitation to write it and Allison Doerr for the editing after Rita left...
www.nature.com/articles/s41...
Targeted RNA-binding protein degradation to improve mRNA live imaging in animal cells - Nature Methods
The gold standard system for mRNA imaging in live cells just got upgraded by being degraded. This development improves signal-to-noise ratios and may lead to a better understanding of mRNA transport a...
www.nature.com
December 17, 2025 at 11:13 AM
My "News & views" was published today @natmethods.nature.com
Thanks @ritastrack.bsky.social for the invitation to write it and Allison Doerr for the editing after Rita left...
www.nature.com/articles/s41...
Thanks @ritastrack.bsky.social for the invitation to write it and Allison Doerr for the editing after Rita left...
www.nature.com/articles/s41...
Reposted by Heilemann Lab
The last chapter from my postdoc days is finally online! We solve structures of the ER membrane O-mannosyltransferase Pmt4 and uncover a functionally important cytosolic binding site for the Dol-P-Man substrate lipid - allosteric regulation or a long sought after Dol-P-Man flippase?? 🔄 rdcu.be/eUJfb
December 16, 2025 at 10:12 AM
The last chapter from my postdoc days is finally online! We solve structures of the ER membrane O-mannosyltransferase Pmt4 and uncover a functionally important cytosolic binding site for the Dol-P-Man substrate lipid - allosteric regulation or a long sought after Dol-P-Man flippase?? 🔄 rdcu.be/eUJfb
Reposted by Heilemann Lab
New paper from @zhixingchen2.bsky.social's lab!
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
December 16, 2025 at 12:11 PM
New paper from @zhixingchen2.bsky.social's lab!
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
Reposted by Heilemann Lab
6SiR-C3-NextA is bright far-red, cell-permeable probe for live-cell selective HDAC6 imaging. Congratulations to Van-Thang Nguyen et al. @vanthang.bsky.social from @imprsgs.bsky.social ! Developed at @mpi-nat.bsky.social nat.bsky.social and published on BioRxiv www.biorxiv.org/content/10.6... .
December 15, 2025 at 6:50 AM
6SiR-C3-NextA is bright far-red, cell-permeable probe for live-cell selective HDAC6 imaging. Congratulations to Van-Thang Nguyen et al. @vanthang.bsky.social from @imprsgs.bsky.social ! Developed at @mpi-nat.bsky.social nat.bsky.social and published on BioRxiv www.biorxiv.org/content/10.6... .
Reposted by Heilemann Lab
We’re excited to announce the opening of the Geneva Expansion Microscopy Facility (GenExM)!
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
December 10, 2025 at 8:43 PM
We’re excited to announce the opening of the Geneva Expansion Microscopy Facility (GenExM)!
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
Reposted by Heilemann Lab
Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Super-resolved Imaging of Molecular Interactions Using FRET-SOFI
Super-resolution fluorescence microscopy is a powerful method to image molecular processes that occur beyond the diffraction limit of light. Förster resonance energy transfer (FRET) is widely used to ...
pubs.acs.org
December 10, 2025 at 9:00 AM
Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by Heilemann Lab
We are recruiting a tenure track professor in the broader area of molecular biology @zmbh.uni-heidelberg.de at Heidelberg University! www.nature.com/naturecareer... Please share and apply!
Tenure Track Professorship (W1 with Tenure Track to W3) in “Molecular Biology" (f/m/d) - Heidelberg job with Universität Heidelberg | 12850668
The Center for Molecular Biology of Heidelberg University (ZMBH) and the Faculty of Biosciences invite applications for a Tenure Track Professors...
www.nature.com
December 9, 2025 at 6:11 PM
We are recruiting a tenure track professor in the broader area of molecular biology @zmbh.uni-heidelberg.de at Heidelberg University! www.nature.com/naturecareer... Please share and apply!
Reposted by Heilemann Lab
Janelia is hiring! "Invent new imaging methods, molecular tools, or protein chemistry approaches"
www.janelia.org/content/lead...
www.janelia.org/content/lead...
Lead a lab at Janelia
www.janelia.org
December 8, 2025 at 8:41 PM
Janelia is hiring! "Invent new imaging methods, molecular tools, or protein chemistry approaches"
www.janelia.org/content/lead...
www.janelia.org/content/lead...
Reposted by Heilemann Lab
Our Science paper is out!
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
December 5, 2025 at 9:47 AM
Our Science paper is out!
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Reposted by Heilemann Lab
The Frankfurt Institute for Advanced Studies (FIAS) and the newly funded Cluster of Excellence SCALE are seeking several Independent Research Group Leaders to drive theoretical and computational research in molecular and cellular life science.
fias.institute/de/das-fias/...
fias.institute/de/das-fias/...
Independent Research Group Leaders (f/m/d) SCALE / FIAS
fias.institute
December 5, 2025 at 5:30 PM
The Frankfurt Institute for Advanced Studies (FIAS) and the newly funded Cluster of Excellence SCALE are seeking several Independent Research Group Leaders to drive theoretical and computational research in molecular and cellular life science.
fias.institute/de/das-fias/...
fias.institute/de/das-fias/...
We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...
November 28, 2025 at 3:12 PM
We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...
🚨🔬A great opportunity to work with high-end microscopy in an exciting scientific environment. Please re-post 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social
👇🏻
👇🏻
A great opportunity to join our newly established imaging centre in Frankfurt as engineer/technician. If you love light microscopy, please apply!
Please share!
Please share!
November 20, 2025 at 4:33 PM
🚨🔬A great opportunity to work with high-end microscopy in an exciting scientific environment. Please re-post 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social
👇🏻
👇🏻
Reposted by Heilemann Lab
Physical principles have redefined our understanding of autophagy.
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
Autophagy regulation by phase separation, avidity, and wetting
Autophagy enables cells to selectively degrade a wide range of macromolecules, and
how this process achieves spatial precision within the densely packed cytosol is an
active area of investigation. Rec...
www.cell.com
November 20, 2025 at 1:34 PM
Physical principles have redefined our understanding of autophagy.
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
Reposted by Heilemann Lab
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Heilemann Lab
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Bidirectional communication between nucleotide and substrate binding sites in a type IV multidrug ABC transporter
Nature Communications - The communication hinge, found in type IV ABC transporters, forms a bidirectional communication between ATP- and substrate-binding sites. This is an unexplored allosteric...
rdcu.be
November 12, 2025 at 11:15 AM
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Reposted by Heilemann Lab
Reposted by Heilemann Lab
In two weeks, our #qbio #Master students learned to build, from drawing to full realization, their own : confocal FCS, TIRF microscope, and optical tweezers setup ! This is hand-on #microscopy !
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
November 8, 2025 at 11:45 AM
In two weeks, our #qbio #Master students learned to build, from drawing to full realization, their own : confocal FCS, TIRF microscope, and optical tweezers setup ! This is hand-on #microscopy !
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Reposted by Heilemann Lab
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 6, 2025 at 5:20 PM
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Heilemann Lab
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Synthetic fluorophores for live-cell fluorescence microscopy and biosensing - Nature Chemical Biology
Performing live-cell microscopy experiments with high spatial and temporal resolution requires fluorophores with highly optimized properties. This Review examines the progress in developing synthetic ...
www.nature.com
November 5, 2025 at 9:29 PM
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Reposted by Heilemann Lab
🔬🤖 ICYMI: impressive preprint from @owenlabuob.bsky.social tackling a major bottleneck – how to systematically compare super-resolution microscopy across experiments. Novel ML framework embeds single-molecule data for cross-sample analysis. Integrated with nano-org database✨ doi.org/10.1101/2025...
November 3, 2025 at 5:57 PM
🔬🤖 ICYMI: impressive preprint from @owenlabuob.bsky.social tackling a major bottleneck – how to systematically compare super-resolution microscopy across experiments. Novel ML framework embeds single-molecule data for cross-sample analysis. Integrated with nano-org database✨ doi.org/10.1101/2025...
Reposted by Heilemann Lab
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
2026 Single Molecule Approaches to Biology Conference GRC
The 2026 Gordon Research Conference on Single Molecule Approaches to Biology will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
www.grc.org
November 2, 2025 at 7:23 PM
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
Reposted by Heilemann Lab
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
October 31, 2025 at 9:24 AM
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
👇🏻 great opportunity for a PhD, great environment & PI!
🚨 #PhD Opportunities 🚨
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
November 1, 2025 at 8:25 AM
👇🏻 great opportunity for a PhD, great environment & PI!