Antonino Cassotta
@cassotta.bsky.social
Human Immunology | Dum loquimur fugerit invida aetas: carpe diem, quam minimum credula postero
https://www.cassottalab.org/
https://www.cassottalab.org/
Reposted by Antonino Cassotta
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
October 28, 2025 at 8:06 AM
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Reposted by Antonino Cassotta
Researchers in #ScienceSignaling have discovered a signaling mechanism that prevents T helper 17 cells from adopting pathogenic states linked to #autoimmunity, potentially informing future efforts to treat autoimmune diseases such as multiple sclerosis. https://scim.ag/4mzj6tX
Adenosine 2A receptor–dependent activation of AMPK represses TH17 cell pathogenicity through epigenetic and metabolic reprogramming
Epigenetic reprogramming that promotes oxidative metabolism suppresses TH17 cell pathogenicity.
scim.ag
September 24, 2025 at 2:29 PM
Researchers in #ScienceSignaling have discovered a signaling mechanism that prevents T helper 17 cells from adopting pathogenic states linked to #autoimmunity, potentially informing future efforts to treat autoimmune diseases such as multiple sclerosis. https://scim.ag/4mzj6tX
Reposted by Antonino Cassotta
We are all somatic mutation mosaics.
"There are trillions of cells in a human body and so the total number of somatic mutations acquired in a single individual may well exceed quadrillions, millions of times the size of the human genome."
@nature.com
nature.com/articles/s41...
"There are trillions of cells in a human body and so the total number of somatic mutations acquired in a single individual may well exceed quadrillions, millions of times the size of the human genome."
@nature.com
nature.com/articles/s41...
The Somatic Mosaicism across Human Tissues Network - Nature
The Somatic Mosaicism across Human Tissues Network aims to create a reference catalogue of somatic mosaicism across different tissues and cells within individuals.
nature.com
July 2, 2025 at 4:21 PM
We are all somatic mutation mosaics.
"There are trillions of cells in a human body and so the total number of somatic mutations acquired in a single individual may well exceed quadrillions, millions of times the size of the human genome."
@nature.com
nature.com/articles/s41...
"There are trillions of cells in a human body and so the total number of somatic mutations acquired in a single individual may well exceed quadrillions, millions of times the size of the human genome."
@nature.com
nature.com/articles/s41...
Reposted by Antonino Cassotta
Being open to new ideas is the only personality trait that is proven to influence creativity and discoveries.
June 1, 2025 at 11:13 AM
Being open to new ideas is the only personality trait that is proven to influence creativity and discoveries.
Inspiring editorial. Understanding immunity, one patient at a time. @jhumimmunity.org
rupress.org/jhi/article/...
rupress.org/jhi/article/...
Human immunity | Journal of Human Immunity | Rockefeller University Press
Human immunity is often insufficient against infectious agents and sometimes over-reactive to innocuous components of the environment or the human body. Ge
rupress.org
May 7, 2025 at 10:10 AM
Inspiring editorial. Understanding immunity, one patient at a time. @jhumimmunity.org
rupress.org/jhi/article/...
rupress.org/jhi/article/...
Learning the language of T cell receptors through large-scale screening: Cancer Cell www.cell.com/cancer-cell/...
Learning the language of T cell receptors through large-scale screening
T cells perform critical roles in orchestrating immunity in health and disease. However,
decoding what individual T cells recognize has long been challenging due to the immense
diversity of both T cel...
www.cell.com
April 23, 2025 at 6:18 PM
Learning the language of T cell receptors through large-scale screening: Cancer Cell www.cell.com/cancer-cell/...
Reposted by Antonino Cassotta
'(...) German research institute BioMed X is stepping in to offer a lifeline. The institute has opened a matchmaking program to align threatened NIH grants with potential new funders from the pharmaceutical industry.'
www.fiercebiotech.com/research/ger...
www.fiercebiotech.com/research/ger...
German research institute launches program to match threatened NIH grants with pharma funders
As scientists across the U.S. face increasing uncertainty due to funding freezes and mass layoffs at health agencies, German research institute BioMed X is stepping in to offer a lifeline. | As scient...
www.fiercebiotech.com
April 12, 2025 at 12:33 PM
'(...) German research institute BioMed X is stepping in to offer a lifeline. The institute has opened a matchmaking program to align threatened NIH grants with potential new funders from the pharmaceutical industry.'
www.fiercebiotech.com/research/ger...
www.fiercebiotech.com/research/ger...
Reposted by Antonino Cassotta
Regulatory T cells with engineered specificity for HLA-DQ2.5–restricted deamidated gluten peptides exert bystander suppression in vivo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
March 19, 2025 at 6:16 PM
Regulatory T cells with engineered specificity for HLA-DQ2.5–restricted deamidated gluten peptides exert bystander suppression in vivo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Antonino Cassotta
Reposted by Antonino Cassotta
Reposted by Antonino Cassotta
Terminal differentiation and persistence of effector regulatory T cells essential for preventing intestinal inflammation @natimmunol.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 6, 2025 at 2:07 AM
Terminal differentiation and persistence of effector regulatory T cells essential for preventing intestinal inflammation @natimmunol.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Antonino Cassotta
In this review, Pilar Martín and Francisco Sánchez-Madrid describe the functions of T cells in cardiac health and disease and discuss implications for cardiac immunotherapies, with a focus on the immune receptor CD69 as a promising target: https://www.jci.org/articles/view/185218
January 22, 2025 at 4:33 PM
In this review, Pilar Martín and Francisco Sánchez-Madrid describe the functions of T cells in cardiac health and disease and discuss implications for cardiac immunotherapies, with a focus on the immune receptor CD69 as a promising target: https://www.jci.org/articles/view/185218
Reposted by Antonino Cassotta
Elucidating the role of autoreactive T cells and B cells in autoimmune hepatitis
@jclinical-invest.bsky.social
www.jci.org/articles/vie...
www.jci.org/articles/vie...
@jclinical-invest.bsky.social
www.jci.org/articles/vie...
www.jci.org/articles/vie...
January 16, 2025 at 10:54 PM
Elucidating the role of autoreactive T cells and B cells in autoimmune hepatitis
@jclinical-invest.bsky.social
www.jci.org/articles/vie...
www.jci.org/articles/vie...
@jclinical-invest.bsky.social
www.jci.org/articles/vie...
www.jci.org/articles/vie...
Reposted by Antonino Cassotta
Xin Chen, Mark Davis et al in @natimmunol.bsky.social study on human CD4+ and CD8+ Tregs using a Foxp3 KO approach in human tonsil-derived immune organoids.
#Immunology #Immunosky #organoids
www.nature.com/articles/s41...
#Immunology #Immunosky #organoids
www.nature.com/articles/s41...
Differential roles of human CD4+ and CD8+ regulatory T cells in controlling self-reactive immune responses - Nature Immunology
Here the authors use a tonsil organoid culture model system to investigate the roles of human CD4+ and CD8+ regulatory T cells in controlling self-reactive immune responses. CD4+ regulatory T cells we...
www.nature.com
January 14, 2025 at 10:37 PM
Xin Chen, Mark Davis et al in @natimmunol.bsky.social study on human CD4+ and CD8+ Tregs using a Foxp3 KO approach in human tonsil-derived immune organoids.
#Immunology #Immunosky #organoids
www.nature.com/articles/s41...
#Immunology #Immunosky #organoids
www.nature.com/articles/s41...
“Just because Bluesky is at the earliest phase, before enshittification has started, doesn’t mean it won’t be enshittified.”
Great piece by @kakape.bsky.social about BlueSky and the growth of its academic community
Feat. @lorenzspreen.bsky.social @carlbergstrom.com @jbakcoleman.bsky.social and others, as well as my efforts to monitor and analyze the network out of the equilibrium regime 👇
www.science.org/content/arti...
Feat. @lorenzspreen.bsky.social @carlbergstrom.com @jbakcoleman.bsky.social and others, as well as my efforts to monitor and analyze the network out of the equilibrium regime 👇
www.science.org/content/arti...
As academic Bluesky grows, researchers find strengths—and shortcomings
Platform fosters collegial interactions among scientists, but potentially limits interactions beyond the academic community
www.science.org
January 7, 2025 at 11:30 PM
“Just because Bluesky is at the earliest phase, before enshittification has started, doesn’t mean it won’t be enshittified.”
Reposted by Antonino Cassotta
🌟Keystone Cancer Immunotherapy conference 2025 🌟 Speaker Highlight #2 at #KSCancerImmune25: Dr. Ton N. Schumacher @nkinl.bsky.social will discuss how T cells detect tumors in “Dissecting T Cell Recognition of Cancer.” Don’t miss his cutting-edge immunology insights!
January 6, 2025 at 11:07 PM
🌟Keystone Cancer Immunotherapy conference 2025 🌟 Speaker Highlight #2 at #KSCancerImmune25: Dr. Ton N. Schumacher @nkinl.bsky.social will discuss how T cells detect tumors in “Dissecting T Cell Recognition of Cancer.” Don’t miss his cutting-edge immunology insights!
Reposted by Antonino Cassotta
Ants best humans at test of collective intelligence
"When people work together, they can achieve great things. But if they can’t talk, they’re not necessarily smarter than ants"
Original paper
www.pnas.org/doi/10.1073/...
News
www.science.org/content/arti...
"When people work together, they can achieve great things. But if they can’t talk, they’re not necessarily smarter than ants"
Original paper
www.pnas.org/doi/10.1073/...
News
www.science.org/content/arti...
January 5, 2025 at 9:41 AM
Ants best humans at test of collective intelligence
"When people work together, they can achieve great things. But if they can’t talk, they’re not necessarily smarter than ants"
Original paper
www.pnas.org/doi/10.1073/...
News
www.science.org/content/arti...
"When people work together, they can achieve great things. But if they can’t talk, they’re not necessarily smarter than ants"
Original paper
www.pnas.org/doi/10.1073/...
News
www.science.org/content/arti...
Reposted by Antonino Cassotta
Receptor-binding proteins from animal viruses are broadly compatible with human cell entry factors
@naturemicrobiol.bsky.social
www.nature.com/articles/s41...
@naturemicrobiol.bsky.social
www.nature.com/articles/s41...
January 3, 2025 at 6:51 PM
Receptor-binding proteins from animal viruses are broadly compatible with human cell entry factors
@naturemicrobiol.bsky.social
www.nature.com/articles/s41...
@naturemicrobiol.bsky.social
www.nature.com/articles/s41...
Reposted by Antonino Cassotta
‼️‼️
Last few days to apply to join us as a
#PostDoc in #SystemsImmunology and explore mechanisms of #SexDifferences in #Human #Immunity at Lund University in #Sweden
More info on how to apply below (please RT)
Last few days to apply to join us as a
#PostDoc in #SystemsImmunology and explore mechanisms of #SexDifferences in #Human #Immunity at Lund University in #Sweden
More info on how to apply below (please RT)
Join our #SystemsImmunology Lab (Lund University, Sweden) as a #Postdoctoral Fellow!
🔬 Project: To investigate sex hormone regulation of human immune function using CRISPR, transcriptomics, and immunophenotyping. Fully funded 2-year position (more info attached).
🗓️ Apply by Jan 6, 2025
🔬 Project: To investigate sex hormone regulation of human immune function using CRISPR, transcriptomics, and immunophenotyping. Fully funded 2-year position (more info attached).
🗓️ Apply by Jan 6, 2025
January 3, 2025 at 10:51 AM
‼️‼️
Last few days to apply to join us as a
#PostDoc in #SystemsImmunology and explore mechanisms of #SexDifferences in #Human #Immunity at Lund University in #Sweden
More info on how to apply below (please RT)
Last few days to apply to join us as a
#PostDoc in #SystemsImmunology and explore mechanisms of #SexDifferences in #Human #Immunity at Lund University in #Sweden
More info on how to apply below (please RT)
Reposted by Antonino Cassotta
Hello T cell enthusiasts! Ever tried to combine an AIM and ICS assay? If not, here is the protocol for you!
academic.oup.com/ooim/advance...
If you're into T cell analysis methodologies, do take a look at this super paper from Alba Grifoni and colleagues
( - sadly she's still on social media at the Other Place, but hopefully soon on @bsky.app)
If you're into T cell analysis methodologies, do take a look at this super paper from Alba Grifoni and colleagues
( - sadly she's still on social media at the Other Place, but hopefully soon on @bsky.app)
In-depth characterization of T cell responses with a combined Activation-Induced Marker (AIM) and Intracellular Cytokine Staining (ICS) assay
Abstract. Since T cells are key mediators in the adaptive immune system, evaluating antigen-specific T cell immune responses is pivotal to understanding im
academic.oup.com
December 23, 2024 at 3:54 PM
Hello T cell enthusiasts! Ever tried to combine an AIM and ICS assay? If not, here is the protocol for you!
Beautiful!!
#Immunology out now @science.org
Biased antibody responses to influenza strains in vaccines are strongly linked to genetics. Coupling antigens from different strains together may broaden antibody responses.
https://buff.ly/4flV5TK
By Vamsee Mallajosyula, Mark Davis et al.
Biased antibody responses to influenza strains in vaccines are strongly linked to genetics. Coupling antigens from different strains together may broaden antibody responses.
https://buff.ly/4flV5TK
By Vamsee Mallajosyula, Mark Davis et al.
December 21, 2024 at 10:24 PM
Beautiful!!
Reposted by Antonino Cassotta
1/ In two back-to-back papers, we present our de novo TRACeR platform for targeting MHC-I and MHC-II antigens
TRACeR for MHC-I: go.nature.com/4gcLzn5
TRACeR for MHC-II: go.nature.com/4gj5OQk
TRACeR for MHC-I: go.nature.com/4gcLzn5
TRACeR for MHC-II: go.nature.com/4gj5OQk
December 17, 2024 at 12:56 AM
1/ In two back-to-back papers, we present our de novo TRACeR platform for targeting MHC-I and MHC-II antigens
TRACeR for MHC-I: go.nature.com/4gcLzn5
TRACeR for MHC-II: go.nature.com/4gj5OQk
TRACeR for MHC-I: go.nature.com/4gcLzn5
TRACeR for MHC-II: go.nature.com/4gj5OQk
Reposted by Antonino Cassotta
I've created a new plugin for #PyMOL (called PyMOLfold) that allows you to fold protein sequences right in the GUI. Simply select your model of choice (e.g., #ESM3, Boltz-1) and paste in your amino acid sequence.
github.com/colbyford/Py...
#ai #proteinfolding #alphafold
github.com/colbyford/Py...
#ai #proteinfolding #alphafold
December 13, 2024 at 1:46 PM
I've created a new plugin for #PyMOL (called PyMOLfold) that allows you to fold protein sequences right in the GUI. Simply select your model of choice (e.g., #ESM3, Boltz-1) and paste in your amino acid sequence.
github.com/colbyford/Py...
#ai #proteinfolding #alphafold
github.com/colbyford/Py...
#ai #proteinfolding #alphafold
Reposted by Antonino Cassotta
December 10, 2024 at 5:55 PM