Here, the authors show that peroxisome proliferation is a conserved response to proteotoxic stress that occurs through stimulation of biogenesis and mediated by TOR and HSR signaling. Importantly, per...

Peroxisomes are membrane-bound organelles harboring metabolic enzymes. In humans, peroxisomes are re

Peroxisomes are central metabolic organelles required for the synthesis of sterols, plasmalogens and certain amino acids, as well as the breakdown of diverse lipids, reactive oxygen species and nucle…

Perox-per-cell automates cumbersome, image-based data collection tasks often encountered in peroxiso

Maintenance of mitochondrial redox homeostasis is of fundamental importance to cellular health. Mitochondria harbor a host of intrinsic antioxidant defenses, but the contribution of extrinsic, nonmito...

Peroxisomes and pexophagy have gained increasing attention in their role within the central nervous system (CNS) in recent years. In this review, we c…

Peroxisomes are integral metabolic organelles involved in both catabolic and anabolic processes in h

Peroxisome de novo biogenesis requires yet unidentified mitochondrial proteins. We report that the o

Science, Volume 389, Issue 6756 , Page 157-162, July 2025.

Repurposing an organelle for specialized metabolism provides an avenue for fermentable, unicellular organisms such as Saccharomyces cerevisiae to mimic compartmentalization of metabolic pathways within different plant tissues. Peroxisomes are attractive organelles for repurposing as they are not required for yeast viability when grown on glucose and can efficiently compartmentalize heterologous enzymes to enable physical separation of cytosolic native metabolism and peroxisomal engineered metabolism. However, when not required, peroxisomes are repressed, leading to low functional capacities for heterologous proteins. Here we engineer peroxisomes with enhanced functional capacities, with the goal of compartmentalizing up to eight metabolic enzymes to enhance titers. We implement a machine learning pipeline that allows the identification of factors to overexpress, culminating in a 137% increase in peroxisome functional capacity compared to a wild-type strain. Improved pathway compartment