Tyler Stanage
@tstanage.bsky.social
Postdoc in the Boulton Lab at @crick.ac.uk. Formerly at UW-Madison and IU-Bloomington. Interested in DNA replication and repair.
Reposted by Tyler Stanage
‼️ Excited to share our new paper out now in @science.org ‼️
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
November 7, 2025 at 10:15 AM
‼️ Excited to share our new paper out now in @science.org ‼️
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
Reposted by Tyler Stanage
🚀 New preprint alert!
We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.
📄 Read the manuscript here: www.biorxiv.org/content/10.1...
We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.
📄 Read the manuscript here: www.biorxiv.org/content/10.1...
SLFN11 restricts escape from telomere crisis to prevent alternative lengthening of telomeres
The tRNA nuclease SLFN11 is epigenetically silenced in ~50% of treatment-naive tumours and is the strongest predictor of chemoresistance but why it is frequently inactivated in cancer is unknown. To a...
www.biorxiv.org
September 11, 2025 at 7:23 AM
🚀 New preprint alert!
We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.
📄 Read the manuscript here: www.biorxiv.org/content/10.1...
We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.
📄 Read the manuscript here: www.biorxiv.org/content/10.1...
Reposted by Tyler Stanage
1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....
Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage - Nature Cell Biology
Szmyd et al. show that DNA repair pathways impact whether cells with DNA lesions arrest in mitosis. The formation of homologous recombination-driven double Holliday junctions elicits mitotic cell deat...
www.nature.com
January 13, 2025 at 10:12 AM
1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....
Reposted by Tyler Stanage
NEW in @science.org, so happy to contribute to our understanding of the molecular machine, Fidgetin-like 1 (FIGNL1), and elucidating the mechanism of why cells cannot survive when it is lost! #ScienceResearch science.org/doi/10.1126/... (1/7)
Molecular basis of FIGNL1 in dissociating RAD51 from DNA and chromatin
Maintaining genome integrity is an essential and challenging process. RAD51 recombinase, the central player of several crucial processes in repairing DNA and protecting genome integrity, forms filamen...
science.org
December 5, 2024 at 7:32 PM
NEW in @science.org, so happy to contribute to our understanding of the molecular machine, Fidgetin-like 1 (FIGNL1), and elucidating the mechanism of why cells cannot survive when it is lost! #ScienceResearch science.org/doi/10.1126/... (1/7)
Reposted by Tyler Stanage
Hello, Bluesky!
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
December 3, 2024 at 1:00 PM
Hello, Bluesky!
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
Reposted by Tyler Stanage
📢 Make your work FASTER and EASIER!
Use AlphaBridge to analyze your AlphaFold results and receive a clear, easy-to-understand output—even for non-experts.
➡️ Let AlphaBridge help you accelerate your workflow!
❤️ Share and try it here: alpha-bridge.eu
👀Read more: www.biorxiv.org/content/10.1...
Use AlphaBridge to analyze your AlphaFold results and receive a clear, easy-to-understand output—even for non-experts.
➡️ Let AlphaBridge help you accelerate your workflow!
❤️ Share and try it here: alpha-bridge.eu
👀Read more: www.biorxiv.org/content/10.1...
December 3, 2024 at 11:15 PM
📢 Make your work FASTER and EASIER!
Use AlphaBridge to analyze your AlphaFold results and receive a clear, easy-to-understand output—even for non-experts.
➡️ Let AlphaBridge help you accelerate your workflow!
❤️ Share and try it here: alpha-bridge.eu
👀Read more: www.biorxiv.org/content/10.1...
Use AlphaBridge to analyze your AlphaFold results and receive a clear, easy-to-understand output—even for non-experts.
➡️ Let AlphaBridge help you accelerate your workflow!
❤️ Share and try it here: alpha-bridge.eu
👀Read more: www.biorxiv.org/content/10.1...
Reposted by Tyler Stanage
Another week, another great paper! Kudos to the amazing grad student, Chiara Masnovo who single-handedly initiated and led the project, and arranged a terrific collaboration with the Aharoni lab from Ben-Gurion University, and to the great members of the two labs.
www.nature.com/articles/s41...?
Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability - Nature Communications
DNA repeats can lengthen or shorten during their replication, which may lead to a human disease. Here, the authors discovered that an essential replication protein, Mcm10, counteracts instability of G...
www.nature.com
December 4, 2024 at 12:34 AM
Another week, another great paper! Kudos to the amazing grad student, Chiara Masnovo who single-handedly initiated and led the project, and arranged a terrific collaboration with the Aharoni lab from Ben-Gurion University, and to the great members of the two labs.
www.nature.com/articles/s41...?
Reposted by Tyler Stanage
H3-H4 histone dimers act as copper reductase. This is important because ETC and SOD1 require bio-usable Cu+ for function. This article shows a new mechanism by which chromatin histones can affect the cell metabolism via a new role in metal processing #redoxepigenetics www.science.org/doi/10.1126/...
The histone H3-H4 tetramer is a copper reductase enzyme
A eukaryotic histone complex can package DNA but is also a cupric reductase that provides biousable Cu1+ for the cell.
www.science.org
December 1, 2024 at 1:34 PM
H3-H4 histone dimers act as copper reductase. This is important because ETC and SOD1 require bio-usable Cu+ for function. This article shows a new mechanism by which chromatin histones can affect the cell metabolism via a new role in metal processing #redoxepigenetics www.science.org/doi/10.1126/...
Reposted by Tyler Stanage
New at NAR Cancer: Ex vivo functional test for HR proficiency applied to a cohort of ovarian cancer PDX models: a way to identify PARPi-sensitive tumors lacking BRCA1/2 alterations. From Francien Talens et al., Univ of Groningen. academic.oup.com/narcancer/ar...
RAD51 recruitment but not replication fork stability associates with PARP inhibitor response in ovarian cancer patient-derived xenograft models
Abstract. Poly(ADP‐ribose) polymerase (PARP) inhibitors (PARPis) are currently used to treat BRCA1/2 mutant cancers. Although PARPi sensitivity has been at
academic.oup.com
November 30, 2024 at 8:08 PM
New at NAR Cancer: Ex vivo functional test for HR proficiency applied to a cohort of ovarian cancer PDX models: a way to identify PARPi-sensitive tumors lacking BRCA1/2 alterations. From Francien Talens et al., Univ of Groningen. academic.oup.com/narcancer/ar...
Reposted by Tyler Stanage
“In this Review, we discuss #telomere protection, maintenance and damage. We highlight the differences between #human and #mouse telomere biology that may contribute to discrepancies between human #diseases and mouse models.” #telomerase #aging
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Telomere function and regulation from mouse models to human ageing and disease - Nature Reviews Molecular Cell Biology
Telomere dysfunction, usually owing to shortening, activates cellular senescence and can contribute to age-associated diseases and cancer. Mouse models are crucial for telomere research, but human and...
www.nature.com
November 30, 2024 at 6:02 AM
“In this Review, we discuss #telomere protection, maintenance and damage. We highlight the differences between #human and #mouse telomere biology that may contribute to discrepancies between human #diseases and mouse models.” #telomerase #aging
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tyler Stanage
Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability https://www.biorxiv.org/content/10.1101/2024.11.26.625192v1
Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability https://www.biorxiv.org/content/10.1101/2024.11.26.625192v1
The DNA-dependent protease SPRTN maintains genome stability by degrading toxic DNA-protein crosslink
www.biorxiv.org
November 28, 2024 at 4:45 PM
Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability https://www.biorxiv.org/content/10.1101/2024.11.26.625192v1
Reposted by Tyler Stanage
"Negative reinforcement, such as receiving overly harsh criticism or being scolded by a research mentor, and being held to unreasonable expectations were two of the most detrimental aspects of research affecting student mental health."
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Behind the graduate mental health crisis in science - Nature Biotechnology
Survey results identify how scientific research and teaching contribute to the graduate student mental health crisis.
www.nature.com
November 27, 2024 at 5:35 AM
"Negative reinforcement, such as receiving overly harsh criticism or being scolded by a research mentor, and being held to unreasonable expectations were two of the most detrimental aspects of research affecting student mental health."
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tyler Stanage
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo
The acute skin reaction to sunburn, encompassing keratinocyte cell death, inflammation,
and epidermal thickening, has traditionally been ascribed to DNA damage responses.
Using a mouse model deficient...
www.cell.com
November 26, 2024 at 10:02 AM
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
Reposted by Tyler Stanage
Thank you @gabriellebrewer.bsky.social for highlighting our work in Nature Reviews Cancer, led by grad student @justeng95.bsky.social.
Check out the original paper in Cell here:
www.cell.com/cell/fulltex...
Here's a summary thread from "the other site": x.com/PeterLyLab/s...
Check out the original paper in Cell here:
www.cell.com/cell/fulltex...
Here's a summary thread from "the other site": x.com/PeterLyLab/s...
November 21, 2024 at 4:23 PM
Thank you @gabriellebrewer.bsky.social for highlighting our work in Nature Reviews Cancer, led by grad student @justeng95.bsky.social.
Check out the original paper in Cell here:
www.cell.com/cell/fulltex...
Here's a summary thread from "the other site": x.com/PeterLyLab/s...
Check out the original paper in Cell here:
www.cell.com/cell/fulltex...
Here's a summary thread from "the other site": x.com/PeterLyLab/s...
Reposted by Tyler Stanage
We invite anyone interested in HIV research to our event marking #WorldAIDSDay.
We’ll hear from five expert speakers, including our own Anabel Guedan, with experience in HIV research, clinical trials, policy and activism.
📅 Friday 29 Nov
🕑 14:15-18:30
www.crick.ac.uk/whats-on/wor...
We’ll hear from five expert speakers, including our own Anabel Guedan, with experience in HIV research, clinical trials, policy and activism.
📅 Friday 29 Nov
🕑 14:15-18:30
www.crick.ac.uk/whats-on/wor...
World AIDS Day: Advances in HIV prevention and treatment
www.crick.ac.uk
November 21, 2024 at 4:42 PM
We invite anyone interested in HIV research to our event marking #WorldAIDSDay.
We’ll hear from five expert speakers, including our own Anabel Guedan, with experience in HIV research, clinical trials, policy and activism.
📅 Friday 29 Nov
🕑 14:15-18:30
www.crick.ac.uk/whats-on/wor...
We’ll hear from five expert speakers, including our own Anabel Guedan, with experience in HIV research, clinical trials, policy and activism.
📅 Friday 29 Nov
🕑 14:15-18:30
www.crick.ac.uk/whats-on/wor...
Reposted by Tyler Stanage
My first post on BlueSky.
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
E3 ligases: a ubiquitous link between DNA repair, DNA replication and human disease
Maintenance of genome stability is of paramount importance for the survival of an organism. However, genomic integrity is constantly being challenged by various endogenous and exogenous processes that...
portlandpress.com
November 18, 2024 at 7:26 AM
My first post on BlueSky.
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Reposted by Tyler Stanage
GS II is here! Please follow the newly arrived folks, and comment/DM to be included!
go.bsky.app/VeCmq6
go.bsky.app/VeCmq6
November 18, 2024 at 6:44 PM
GS II is here! Please follow the newly arrived folks, and comment/DM to be included!
go.bsky.app/VeCmq6
go.bsky.app/VeCmq6
Reposted by Tyler Stanage
Happy to share our new paper with Goran Kokic and the Svejstrup lab in Cell!
We show that STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair.
Great teamwork by Diana, Marta and Paula!
www.cell.com/cell/fulltex...
We show that STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair.
Great teamwork by Diana, Marta and Paula!
www.cell.com/cell/fulltex...
November 16, 2024 at 2:06 PM
Happy to share our new paper with Goran Kokic and the Svejstrup lab in Cell!
We show that STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair.
Great teamwork by Diana, Marta and Paula!
www.cell.com/cell/fulltex...
We show that STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair.
Great teamwork by Diana, Marta and Paula!
www.cell.com/cell/fulltex...