Tatiana Moiseeva
@tat1anamoiseeva.bsky.social
looking at replication origin firing in human cells
Views are my own, this is a personal account
https://moiseevalab.com/
DNA replication, genome stability, DNA polymerase epsilon, cancer research
Views are my own, this is a personal account
https://moiseevalab.com/
DNA replication, genome stability, DNA polymerase epsilon, cancer research
Pinned
ok, Genome Stability people. Here are our two starter packs - since I can't pin them both independently to my profile, have to make a new post. Still adding new people to the second pack. As usual, please reach out if you would like to be added!
bsky.app/starter-pack...
bsky.app/starter-pack...
bsky.app/starter-pack...
bsky.app/starter-pack...
Reposted by Tatiana Moiseeva
Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1 https://www.biorxiv.org/content/10.1101/2025.11.13.688213v1
November 14, 2025 at 3:18 PM
Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1 https://www.biorxiv.org/content/10.1101/2025.11.13.688213v1
Reposted by Tatiana Moiseeva
Check out our new biosensor technology to study DDR kinase signaling: ProKAS.
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
November 13, 2025 at 6:22 PM
Check out our new biosensor technology to study DDR kinase signaling: ProKAS.
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
Reposted by Tatiana Moiseeva
In praise of fundamental research
Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different
🧪
@nature.com
www.nature.com/articles/d41...
Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different
🧪
@nature.com
www.nature.com/articles/d41...
From MRI to Ozempic: breakthroughs that show why fundamental research must be protected
In these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different.
www.nature.com
October 30, 2025 at 1:53 PM
In praise of fundamental research
Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different
🧪
@nature.com
www.nature.com/articles/d41...
Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different
🧪
@nature.com
www.nature.com/articles/d41...
Reposted by Tatiana Moiseeva
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
October 30, 2025 at 10:07 PM
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Tatiana Moiseeva
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers
DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...
www.biorxiv.org
September 20, 2025 at 4:10 PM
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
When your incoming lab member's H1B was approved yesterday, but now they can't travel to the US...
September 20, 2025 at 3:40 PM
When your incoming lab member's H1B was approved yesterday, but now they can't travel to the US...
Thanks CSHL for the photos, my facial expressions on them are priceless. I think this one is a keeper for replying to a lot of stuff on the internets...
September 18, 2025 at 6:23 PM
Thanks CSHL for the photos, my facial expressions on them are priceless. I think this one is a keeper for replying to a lot of stuff on the internets...
Excited to be back at the CSHL meeting on DNA replication this week! Campus views are amazing as always, looking forward to all the science!
September 2, 2025 at 10:31 PM
Excited to be back at the CSHL meeting on DNA replication this week! Campus views are amazing as always, looking forward to all the science!
Reposted by Tatiana Moiseeva
Checkout our latest research in @natcomms.nature.com
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team
A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells
Nature Communications - Determining how replication forks move across the human genome is critical for the effective use of agents that target replication stress. Here, the authors present...
rdcu.be
September 1, 2025 at 10:04 AM
Checkout our latest research in @natcomms.nature.com
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team
Reposted by Tatiana Moiseeva
How are Rad51 filaments formed during Homologous Recombination?
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination
Nature Communications - The mediator protein Rad52 promotes Rad51 binding onto RPA-coated DNA to initiate homologous recombination. Here, the authors show that Rad52 sorts Rad51 into monomers and...
urldefense.com
July 21, 2025 at 12:34 PM
How are Rad51 filaments formed during Homologous Recombination?
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Our new review on how the initiation of DNA replication is coordinated with transcription is now online! Congratulations to @syedshahidmusvi.bsky.social!
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
From cooperation to conflicts – a complicated relationship between transcription and replication.
A tight coordination between transcription and DNA replication is essential to maintain genome stability. Both excessive transcription and upregulated…
www.sciencedirect.com
July 17, 2025 at 2:59 PM
Our new review on how the initiation of DNA replication is coordinated with transcription is now online! Congratulations to @syedshahidmusvi.bsky.social!
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Tatiana Moiseeva
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS
Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic
academic.oup.com
July 10, 2025 at 2:55 PM
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
Reposted by Tatiana Moiseeva
Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...
July 2, 2025 at 5:01 PM
Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...
Sameera Vipat, the first PhD student from our lab, successfully defended her thesis in Tallinn yesterday! Congratulations to Sameera, and a huge thank you to @lmyler.bsky.social for being an opponent and a great scientific discussion!
June 20, 2025 at 7:01 PM
Sameera Vipat, the first PhD student from our lab, successfully defended her thesis in Tallinn yesterday! Congratulations to Sameera, and a huge thank you to @lmyler.bsky.social for being an opponent and a great scientific discussion!
We carried an agarose/EtBr gel to the imager on a plastic box cover that was labeled with red sharpie. The gel acquired a nice but unexpected label. How did this happen? The box was not imaged. Sharpies don't get washed off that easily. What kind of sorcery is this?
The label says "biotin HA 6/6"
The label says "biotin HA 6/6"
June 13, 2025 at 5:58 PM
We carried an agarose/EtBr gel to the imager on a plastic box cover that was labeled with red sharpie. The gel acquired a nice but unexpected label. How did this happen? The box was not imaged. Sharpies don't get washed off that easily. What kind of sorcery is this?
The label says "biotin HA 6/6"
The label says "biotin HA 6/6"
Reposted by Tatiana Moiseeva
🧪 Attention those with terminated grants, including for NSF, NASA, EPA, NIH, etc:
Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela...
Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).
Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela...
Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).
May 27, 2025 at 6:30 PM
🧪 Attention those with terminated grants, including for NSF, NASA, EPA, NIH, etc:
Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela...
Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).
Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela...
Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).
Reposted by Tatiana Moiseeva
Excited to share our latest preprint by @mihyun-kim.bsky.social showing how DNA incision activities in nucleotide excision are regulated to ensure genomic stability. We show that interactions between TFIIH and XPG control the 5' incision by ERCC1-XPF. www.biorxiv.org/content/10.1...
May 25, 2025 at 12:48 PM
Excited to share our latest preprint by @mihyun-kim.bsky.social showing how DNA incision activities in nucleotide excision are regulated to ensure genomic stability. We show that interactions between TFIIH and XPG control the 5' incision by ERCC1-XPF. www.biorxiv.org/content/10.1...
Reposted by Tatiana Moiseeva
Delighted to share that the results of my main postdoc project are now published in @nature.com
🔗 doi.org/10.1038/s415...
🔗 doi.org/10.1038/s415...
Multigenerational cell tracking of DNA replication and heritable DNA damage - Nature
A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early even...
doi.org
May 21, 2025 at 3:27 PM
Delighted to share that the results of my main postdoc project are now published in @nature.com
🔗 doi.org/10.1038/s415...
🔗 doi.org/10.1038/s415...
Reposted by Tatiana Moiseeva
Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...
May 15, 2025 at 12:55 AM
Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...
Hey everyone! Every year our HIllman Cancer Center organizes a fundraising bike ride to raise money for cancer research. Sad, but currently it is especially relevant. If you like this cause, please consider supporting our Genome Stability team!
fundraisers.rushtocrushcancer.org/tatiana-mois...
fundraisers.rushtocrushcancer.org/tatiana-mois...
Rush to Crush Cancer
Hi! I work as an Assistant professor in HIllman Cancer Center, my research lab studies how cancer cells react to DNA damage (llike cancer drugs) and how they double their DNA to grow. As you probably ...
fundraisers.rushtocrushcancer.org
May 9, 2025 at 1:29 PM
Hey everyone! Every year our HIllman Cancer Center organizes a fundraising bike ride to raise money for cancer research. Sad, but currently it is especially relevant. If you like this cause, please consider supporting our Genome Stability team!
fundraisers.rushtocrushcancer.org/tatiana-mois...
fundraisers.rushtocrushcancer.org/tatiana-mois...
Reposted by Tatiana Moiseeva
A huge thank you to our speakers @kajastelab.bsky.social and Raphael Ceccaldi for their amazing talks and fascinating research at yesterday's webinar!
Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social.
Join our community: docs.google.com/forms/d/e/1F...
Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social.
Join our community: docs.google.com/forms/d/e/1F...
May 7, 2025 at 6:16 PM
A huge thank you to our speakers @kajastelab.bsky.social and Raphael Ceccaldi for their amazing talks and fascinating research at yesterday's webinar!
Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social.
Join our community: docs.google.com/forms/d/e/1F...
Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social.
Join our community: docs.google.com/forms/d/e/1F...
Reposted by Tatiana Moiseeva
24th Annual Midwest DNA Repair Symposium
University of Michigan
May 17-18, 2025, Ann Arbor
sites.google.com/umich.edu/um...
University of Michigan
May 17-18, 2025, Ann Arbor
sites.google.com/umich.edu/um...
24th Annual Midwest DNA Repair Symposium
The 24th Annual Midwest DNA Repair Symposium will be held at the University of Michigan in the Kahn Auditorium of the Biomedical Science Research Building (BSRB) on May 17–18, 2025. The event will sta...
sites.google.com
May 2, 2025 at 12:32 PM
24th Annual Midwest DNA Repair Symposium
University of Michigan
May 17-18, 2025, Ann Arbor
sites.google.com/umich.edu/um...
University of Michigan
May 17-18, 2025, Ann Arbor
sites.google.com/umich.edu/um...
Reposted by Tatiana Moiseeva
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
April 6, 2025 at 9:45 AM
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
Reposted by Tatiana Moiseeva
Happy to post our first preprint in a while! www.biorxiv.org/content/10.1.... This was a slightly new direction for us, spearheaded by amazing post-doc @audreylumeau.bsky.social. Short thread to follow 🧬
March 7, 2025 at 4:54 PM
Happy to post our first preprint in a while! www.biorxiv.org/content/10.1.... This was a slightly new direction for us, spearheaded by amazing post-doc @audreylumeau.bsky.social. Short thread to follow 🧬
Reposted by Tatiana Moiseeva
NIGMS Advisory Council for May 22 has finally posted to the Federal Register. May the odds be ever in your favor, friends.
www.federalregister.gov/documents/20...
www.federalregister.gov/documents/20...
a woman is covering her mouth with her hand while holding an arrow in her hand .
Alt: a woman is covering her mouth with her hand while holding an arrow in her hand .
media.tenor.com
March 26, 2025 at 4:41 PM
NIGMS Advisory Council for May 22 has finally posted to the Federal Register. May the odds be ever in your favor, friends.
www.federalregister.gov/documents/20...
www.federalregister.gov/documents/20...