Tanner Fadero
@tanner-fadero.bsky.social
(he/him) Light microscopy scientist interested in developing and deploying better live-cell fluorescence microscopy technology. Tweets about microscopy🔬, cell biology, and optics. Conflicts of interest: http://tinyurl.com/y25ctubo
Motorized slit! Off-the-shelf part from ScienceTech. Thorlabs stepper motor driven by K-cube controller.
www.sciencetech-inc.com/shop/product...
@amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social
www.sciencetech-inc.com/shop/product...
@amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social
November 19, 2025 at 6:06 PM
Motorized slit! Off-the-shelf part from ScienceTech. Thorlabs stepper motor driven by K-cube controller.
www.sciencetech-inc.com/shop/product...
@amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social
www.sciencetech-inc.com/shop/product...
@amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social
Reposted by Tanner Fadero
Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.
October 8, 2025 at 7:57 PM
Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.
Reposted by Tanner Fadero
@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.
September 18, 2025 at 3:02 AM
@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.
Reposted by Tanner Fadero
What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post).
-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!
-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!
September 1, 2025 at 6:20 PM
What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post).
-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!
-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!
Reposted by Tanner Fadero
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
August 28, 2025 at 11:40 PM
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
Reposted by Tanner Fadero
Bluesky is now the platform of choice for the scientific community.
From @jenlucpiquant.bsky.social on @arstechnica.com
arstechnica.com/science/2025...
From @jenlucpiquant.bsky.social on @arstechnica.com
arstechnica.com/science/2025...
Bluesky now platform of choice for science community
It’s not just you. Survey says: “Twitter sucks now and all the cool kids are moving to Bluesky”…
arstechnica.com
August 27, 2025 at 3:34 PM
Bluesky is now the platform of choice for the scientific community.
From @jenlucpiquant.bsky.social on @arstechnica.com
arstechnica.com/science/2025...
From @jenlucpiquant.bsky.social on @arstechnica.com
arstechnica.com/science/2025...
Optics folks: does anyone know a manufacturer of a dichroic beamsplitter *cube*?
Essentially, a dichroic version of this (for fluorescence microscopy):
Essentially, a dichroic version of this (for fluorescence microscopy):
August 28, 2025 at 12:15 AM
Optics folks: does anyone know a manufacturer of a dichroic beamsplitter *cube*?
Essentially, a dichroic version of this (for fluorescence microscopy):
Essentially, a dichroic version of this (for fluorescence microscopy):
Science hive mind!
Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!
Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!
August 21, 2025 at 4:43 PM
Science hive mind!
Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!
Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!
Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy!
Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.
#snouty
Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.
#snouty
August 20, 2025 at 6:57 PM
Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy!
Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.
#snouty
Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.
#snouty
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 7:31 PM
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
Hey @thorlabs.bsky.social! Thanks for such a well-documented APT Controller Communication Protocol! It was fun coding against it.
@andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).
@andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).
August 4, 2025 at 11:48 PM
Hey @thorlabs.bsky.social! Thanks for such a well-documented APT Controller Communication Protocol! It was fun coding against it.
@andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).
@andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).
A @bob-goldstein-art.bsky.social original!
August 4, 2025 at 4:31 PM
A @bob-goldstein-art.bsky.social original!
If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go!
Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.
#snouty
Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.
#snouty
July 26, 2025 at 7:18 PM
If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go!
Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.
#snouty
Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.
#snouty
Reposted by Tanner Fadero
Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social
July 25, 2025 at 3:29 PM
Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social
Reposted by Tanner Fadero
The making of "Mr Snouty"
YouTube video by Microscopist's Nightmares
www.youtube.com
July 9, 2025 at 11:41 PM
Reposted by Tanner Fadero
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
July 9, 2025 at 8:00 PM
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
I spent some time finally deriving the relationship between lateral image magnification and axial magnification! It's an interesting equation, with dependencies on sample refractive index and the wave propogation angle w.r.t. the optical axis.
Play with it yourself! www.desmos.com/calculator/q...
Play with it yourself! www.desmos.com/calculator/q...
July 1, 2025 at 3:59 PM
I spent some time finally deriving the relationship between lateral image magnification and axial magnification! It's an interesting equation, with dependencies on sample refractive index and the wave propogation angle w.r.t. the optical axis.
Play with it yourself! www.desmos.com/calculator/q...
Play with it yourself! www.desmos.com/calculator/q...
@kahsage.bsky.social is killing it with these teaching tools for #snoutscope training at the UCSF Center for Advanced Light Microscopy. Fantastic illustrations and a whiteboard in the room. Brilliant!
June 26, 2025 at 6:57 PM
@kahsage.bsky.social is killing it with these teaching tools for #snoutscope training at the UCSF Center for Advanced Light Microscopy. Fantastic illustrations and a whiteboard in the room. Brilliant!
Reposted by Tanner Fadero
De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...
June 26, 2025 at 1:50 PM
De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...
Reposted by Tanner Fadero
1/27 We have a new paper out! Turns out that snowflake yeast have been hiding a secret from us - they've evolved a (very!) crude circulatory system. Not with blood vessels or a heart, but through spontaneous fluid flows powered by their metabolism. 🧪🔬
www.science.org/doi/full/10....
www.science.org/doi/full/10....
June 24, 2025 at 4:52 PM
1/27 We have a new paper out! Turns out that snowflake yeast have been hiding a secret from us - they've evolved a (very!) crude circulatory system. Not with blood vessels or a heart, but through spontaneous fluid flows powered by their metabolism. 🧪🔬
www.science.org/doi/full/10....
www.science.org/doi/full/10....
Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
June 24, 2025 at 1:31 AM
Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all!
"This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"
"This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"
June 21, 2025 at 2:00 PM
I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all!
"This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"
"This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"
Hey optical tech dev folks: how do you do alignment? Can you outline your alignment procedures for positioning optics and verifying their correct positioning?
June 3, 2025 at 12:45 AM
Hey optical tech dev folks: how do you do alignment? Can you outline your alignment procedures for positioning optics and verifying their correct positioning?