Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy!

Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.

#snouty

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go!

Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.

#snouty

Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab.

Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.

I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all!

"This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"

@jennysachweh.bsky.social inspired me to make a quick and dirty model of "Two Mirror Beam Scanning" this morning. It's a nice visual demonstration of how two galvo mirrors can translate a beam with no angular motion.

Try it for yourself! www.geogebra.org/calculator/s...