Tanner Fadero
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tanner-fadero.bsky.social
Tanner Fadero
@tanner-fadero.bsky.social
(he/him) Light microscopy scientist interested in developing and deploying better live-cell fluorescence microscopy technology. Tweets about microscopy🔬, cell biology, and optics. Conflicts of interest: http://tinyurl.com/y25ctubo
Good point! I've yet to find one with an absolute positional encoder that doesn't need to home. This probably isn't the exception.
November 20, 2025 at 4:36 AM
Yes, there's a nice trick to compensating for transferring possession to the table from the crane. But there's also certain steps you can take before giving possession to the crane that decrease tension, leading to less severe shifts during future transfers. I'd love to walk you through them!
September 23, 2025 at 4:59 PM
the anticipation is killing me
September 18, 2025 at 10:45 PM
September 18, 2025 at 10:44 PM
(I see nothing)
September 18, 2025 at 10:42 PM
@jsdaniel02.bsky.social can make it happen!
September 18, 2025 at 3:39 AM
But yeah, a modest tilt angle between projections looks similar!
August 30, 2025 at 11:25 PM
Oh neat! You're looking at a projection from the "native" angle. Interestingly, the closer your sheet tilt angle is to 0°, the more similar the native projection is to the normal Z projection.
August 30, 2025 at 11:09 PM
If that projection is from the deskew plug-in, then it's a true max intensity Z projection (where Z is O1's optical axis).

The deskew plug-in deskews AND rotates your data.
August 30, 2025 at 6:41 AM
Chroma said no, but thorlabs seems interested!
August 29, 2025 at 11:05 AM
It's playing, you've just learned how to tune it out after so many builds
August 21, 2025 at 2:28 AM
Also, this build doesn't actually take six months. I was mostly waiting on parts!
August 21, 2025 at 2:25 AM
You should get @jsdaniel02.bsky.social to help you add in a 808 laser!
August 20, 2025 at 2:02 PM
I noticed that too! StayGold seems to have a very short triplet lifetime, suggesting that it's already doing a similar triplet-quenching mechanism to what is happening with infrared. StayGold should definitely be a first target for directed evolution. Imagine StayGold with infrared depletion!
August 19, 2025 at 9:24 PM