Raphael Pavani
@raphaelpavani.bsky.social
Scientist at NCI/NIH studying DNA replication and repair. Views are my own.
Reposted by Raphael Pavani
Happy to share our latest research on the BRCA1-BRCTs now online.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
Validate User
academic.oup.com
September 18, 2025 at 8:40 AM
Happy to share our latest research on the BRCA1-BRCTs now online.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
Reposted by Raphael Pavani
Dive into the step-by-step choreography of transcription-coupled DNA repair.
In our new review published in Nature Cell Biology, we explore what we know and what’s next in this exciting field of research!
www.nature.com/articles/s41...
In our new review published in Nature Cell Biology, we explore what we know and what’s next in this exciting field of research!
www.nature.com/articles/s41...
The molecular basis of human transcription-coupled DNA repair - Nature Cell Biology
This Review discusses transcription-coupled DNA repair in response to the stalling of RNA polymerase II on different types of DNA lesions and highlights the molecular details for the different steps i...
www.nature.com
August 5, 2025 at 9:49 AM
Dive into the step-by-step choreography of transcription-coupled DNA repair.
In our new review published in Nature Cell Biology, we explore what we know and what’s next in this exciting field of research!
www.nature.com/articles/s41...
In our new review published in Nature Cell Biology, we explore what we know and what’s next in this exciting field of research!
www.nature.com/articles/s41...
Reposted by Raphael Pavani
Targeting DNA damage in ageing: towards supercharging DNA repair www.nature.com/articles/s41...
rdcu.be/eqQ7R
This new Perspective discusses approaches to pharmacologically mitigate the adverse effects of DNA damage to prevent mutation-driven cancers and mitigate age-related degenerative diseases
rdcu.be/eqQ7R
This new Perspective discusses approaches to pharmacologically mitigate the adverse effects of DNA damage to prevent mutation-driven cancers and mitigate age-related degenerative diseases
Targeting DNA damage in ageing: towards supercharging DNA repair - Nature Reviews Drug Discovery
DNA damage to the somatic genome has been identified as a major cause of ageing. This Perspective provides an overview of current understanding of the role of genome instability in the ageing process ...
www.nature.com
June 13, 2025 at 3:52 PM
Targeting DNA damage in ageing: towards supercharging DNA repair www.nature.com/articles/s41...
rdcu.be/eqQ7R
This new Perspective discusses approaches to pharmacologically mitigate the adverse effects of DNA damage to prevent mutation-driven cancers and mitigate age-related degenerative diseases
rdcu.be/eqQ7R
This new Perspective discusses approaches to pharmacologically mitigate the adverse effects of DNA damage to prevent mutation-driven cancers and mitigate age-related degenerative diseases
Reposted by Raphael Pavani
New twist on BRCA1-mediated DNA recombination repair and tumor suppression
New twist on BRCA1-mediated DNA recombination repair and tumor suppression
Ever since BRCA1 germline mutations were found to confer a strong predisposition to the development of breast and ovarian cancers, there has been great interest in determining how this protein suppresses tumor formation. Through more than two decades of research, it has become clear that BRCA1 safeguards our genome mainly by promoting DNA repair through homologous recombination (HR). This opinion article outlines our evolving view of BRCA1’s role in end resection, an upstream commitment step for HR, and highlights recent discoveries suggesting that the context in which DNA breaks are generated dictates whether BRCA1 is required for end resection. In addition, strong emerging evidence for the tumor-suppressive function of BRCA1 being mediated predominantly by its indispensable role in supporting RAD51-dependent recombination downstream of end resection is discussed.
dlvr.it
June 6, 2025 at 12:02 PM
New twist on BRCA1-mediated DNA recombination repair and tumor suppression
Checkout our lab's new publication where we discuss the latest advances in BRCA1 biology at double-strand breaks, including our recent work on how BRCA1 promotes recombination at replication-associated DNA breaks. Kudos to Dali who led this piece!
www.cell.com/trends/cell-...
www.cell.com/trends/cell-...
New twist on BRCA1-mediated DNA recombination repair and tumor suppression
Ever since BRCA1 germline mutations were found to confer a strong predisposition to
the development of breast and ovarian cancers, there has been great interest in determining
how this protein suppres...
www.cell.com
June 6, 2025 at 3:14 PM
Checkout our lab's new publication where we discuss the latest advances in BRCA1 biology at double-strand breaks, including our recent work on how BRCA1 promotes recombination at replication-associated DNA breaks. Kudos to Dali who led this piece!
www.cell.com/trends/cell-...
www.cell.com/trends/cell-...
Reposted by Raphael Pavani
Excited to share our new paper on Nature - how Ku accommodates Alu expansion in primates by binding to dsRNA, providing a clue for both the high levels of Ku and its essentiality in human cells. Thank @chaolinzhang @hchung03 @LenaSteckelberg More to come www.nature.com/articles/s41...
Ku limits RNA-induced innate immunity to allow Alu-expansion in primates - Nature
Nature - Ku limits RNA-induced innate immunity to allow Alu-expansion in primates
www.nature.com
May 16, 2025 at 11:11 PM
Excited to share our new paper on Nature - how Ku accommodates Alu expansion in primates by binding to dsRNA, providing a clue for both the high levels of Ku and its essentiality in human cells. Thank @chaolinzhang @hchung03 @LenaSteckelberg More to come www.nature.com/articles/s41...
Congrats @archaeon-alex.bsky.social, amazing paper!
That's dramatic (and cool): "archaea species can also form multicellular tissue–like structures when compressive forces are applied... ... These results establish multicellularity as a feature of all three domains of life" www.science.org/doi/10.1126/...
Tissue-like multicellular development triggered by mechanical compression in archaea
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...
www.science.org
April 4, 2025 at 11:47 AM
Congrats @archaeon-alex.bsky.social, amazing paper!
Reposted by Raphael Pavani
Check out our review on DNA end resection! With Raphael Ceccaldi, www.nature.com/articles/s41...
March 25, 2025 at 4:00 PM
Check out our review on DNA end resection! With Raphael Ceccaldi, www.nature.com/articles/s41...
Reposted by Raphael Pavani
How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
www.science.org
March 6, 2025 at 9:30 PM
How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!
Reposted by Raphael Pavani
SAVE THE DATE! The next EMBO | EMBL Symposium on 'DNA replication and genome maintenance: from basic biology to disease' will be held October 20-23, 2026.
Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).
www.embl.org/about/info/c...
Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).
www.embl.org/about/info/c...
DNA replication and genome maintenance: from basic biology to disease
www.embl.org
February 26, 2025 at 3:22 PM
SAVE THE DATE! The next EMBO | EMBL Symposium on 'DNA replication and genome maintenance: from basic biology to disease' will be held October 20-23, 2026.
Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).
www.embl.org/about/info/c...
Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).
www.embl.org/about/info/c...
Reposted by Raphael Pavani
Review from our lab out today: "Roles for the 3D Genome In the cell cycle, DNA replication, and double strand break repair." Written by @scientistkate.bsky.social, with her co-supervisor @piptaberlay.bsky.social and collaborator Mat Jones. www.frontiersin.org/journals/cel...
March 4, 2025 at 10:05 PM
Review from our lab out today: "Roles for the 3D Genome In the cell cycle, DNA replication, and double strand break repair." Written by @scientistkate.bsky.social, with her co-supervisor @piptaberlay.bsky.social and collaborator Mat Jones. www.frontiersin.org/journals/cel...
Reposted by Raphael Pavani
BRCA1 turned 30 🎉
To celebrate, Jos Jonkers and I explored three decades of BRCA1 research - what we’ve learned about its biology, its role in tumorigenesis and future directions to improve the life of BRCA1 mutation carriers.
Now out in Cancer Discovery ⬇️: aacrjournals.org/cancerdiscov...
To celebrate, Jos Jonkers and I explored three decades of BRCA1 research - what we’ve learned about its biology, its role in tumorigenesis and future directions to improve the life of BRCA1 mutation carriers.
Now out in Cancer Discovery ⬇️: aacrjournals.org/cancerdiscov...
Thirty Years of BRCA1: Mechanistic Insights and Their Impact on Mutation Carriers
Abstract. Thirty years ago, the cloning of the first breast cancer susceptibility gene, BRCA1, marked a milestone in our understanding of hereditary breast and ovarian cancers. This discovery initiate...
aacrjournals.org
March 3, 2025 at 9:26 AM
BRCA1 turned 30 🎉
To celebrate, Jos Jonkers and I explored three decades of BRCA1 research - what we’ve learned about its biology, its role in tumorigenesis and future directions to improve the life of BRCA1 mutation carriers.
Now out in Cancer Discovery ⬇️: aacrjournals.org/cancerdiscov...
To celebrate, Jos Jonkers and I explored three decades of BRCA1 research - what we’ve learned about its biology, its role in tumorigenesis and future directions to improve the life of BRCA1 mutation carriers.
Now out in Cancer Discovery ⬇️: aacrjournals.org/cancerdiscov...
Amazing work by Susan’s lab showing that transcription termination/pausing can be a source of endogenous DNA damage and genome instability
What RNA polymerase gets up to in the genome and how mutations happen! Just out in Nature! Many thanks to many collaborators & congrats 1st author Jingjing Liu! www.nature.com/articles/s41...
March 1, 2025 at 5:51 PM
Amazing work by Susan’s lab showing that transcription termination/pausing can be a source of endogenous DNA damage and genome instability
Reposted by Raphael Pavani
Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
www.nature.com
January 30, 2025 at 12:30 PM
Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! www.nature.com/articles/s41...
Reposted by Raphael Pavani
Excited to share our preprint reporting a completely new approach to study kinase signaling: ProKAS.
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
December 17, 2024 at 2:10 PM
Excited to share our preprint reporting a completely new approach to study kinase signaling: ProKAS.
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
Reposted by Raphael Pavani
Ron Milligan, a retired @scripps.edu prof and a pioneer in using cryoEM to advance cell biology, now crafts ceramics—and he’s really good at it! Visited him today and picked up two stunning pieces for my office. One of them will be full of candy, so swing by! 🍬
www.ronmilliganceramics.com/philosophy
www.ronmilliganceramics.com/philosophy
December 7, 2024 at 9:55 PM
Ron Milligan, a retired @scripps.edu prof and a pioneer in using cryoEM to advance cell biology, now crafts ceramics—and he’s really good at it! Visited him today and picked up two stunning pieces for my office. One of them will be full of candy, so swing by! 🍬
www.ronmilliganceramics.com/philosophy
www.ronmilliganceramics.com/philosophy
Reposted by Raphael Pavani
Mcm10 — an important replication and elongation factor, counteracts repeat instability and ensures survival of yeast cells containing large homopurine homopyrimidine repeats, such as GAA associated with Friedreich’s Ataxia and AAGGG associated with CANVAS. 🧬 @natureportfolio.bsky.social
Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability
Nature Communications - DNA repeats can lengthen or shorten during their replication, which may lead to a human disease. Here, the authors discovered that an essential replication protein, Mcm10,...
rdcu.be
December 4, 2024 at 12:30 PM
Mcm10 — an important replication and elongation factor, counteracts repeat instability and ensures survival of yeast cells containing large homopurine homopyrimidine repeats, such as GAA associated with Friedreich’s Ataxia and AAGGG associated with CANVAS. 🧬 @natureportfolio.bsky.social
Reposted by Raphael Pavani
Delighted to publish my new molecular animation:
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
DNA Break Repair by Homologous Recombination (2024) Drew Berry wehi.tv
YouTube video by WEHImovies
youtu.be
December 4, 2024 at 12:07 AM
Delighted to publish my new molecular animation:
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs