@lilili-lll.bsky.social
Reposted
New paper out 🔥!
Small promoter, big potential🧬 We show that the ultra-compact Calm1 promoter efficiently targets human neurons in brain organoids and is a promising alternative to hSyn for AAV gene delivery.
Huge thanks to everyone who contributed to this work!
🧠 📄: www.nature.com/articles/s41...
Small promoter, big potential🧬 We show that the ultra-compact Calm1 promoter efficiently targets human neurons in brain organoids and is a promising alternative to hSyn for AAV gene delivery.
Huge thanks to everyone who contributed to this work!
🧠 📄: www.nature.com/articles/s41...
Compact Calm1 promoter enables AAV mediated neuron-targeted expression in human iPSC-derived brain organoids - Scientific Reports
Scientific Reports - Compact Calm1 promoter enables AAV mediated neuron-targeted expression in human iPSC-derived brain organoids
www.nature.com
January 5, 2026 at 12:37 PM
New paper out 🔥!
Small promoter, big potential🧬 We show that the ultra-compact Calm1 promoter efficiently targets human neurons in brain organoids and is a promising alternative to hSyn for AAV gene delivery.
Huge thanks to everyone who contributed to this work!
🧠 📄: www.nature.com/articles/s41...
Small promoter, big potential🧬 We show that the ultra-compact Calm1 promoter efficiently targets human neurons in brain organoids and is a promising alternative to hSyn for AAV gene delivery.
Huge thanks to everyone who contributed to this work!
🧠 📄: www.nature.com/articles/s41...
Reposted
Great news for all the ExM community! A full palette of DNA probes compatible with Expansion microscopy from @spirochrome.com 🧪
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
January 8, 2026 at 3:12 PM
Great news for all the ExM community! A full palette of DNA probes compatible with Expansion microscopy from @spirochrome.com 🧪
Reposted
Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇
www.nature.com/articles/s41...
More info 👇
Membrane editing with proximity labeling reveals regulators of lipid homeostasis - Nature Chemical Biology
Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.
www.nature.com
January 7, 2026 at 2:59 PM
Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇
www.nature.com/articles/s41...
More info 👇
Reposted
Good to see the peer reviewed version of this published
A chemo-optogenetic system for spatiotemporal control of gene expression
We use it to:
- Reconstruct SHH mediated neural tube patterning in vitro
- Measure extracellular half-life of Shh
www.cell.com/developmenta...
A chemo-optogenetic system for spatiotemporal control of gene expression
We use it to:
- Reconstruct SHH mediated neural tube patterning in vitro
- Measure extracellular half-life of Shh
www.cell.com/developmenta...
Investigating morphogen and patterning dynamics with optogenetic control of morphogen production
Benzinger et al. develop a chemo-optogenetic system for precise spatiotemporal control
of morphogen production in vitro. Using light-controlled Sonic hedgehog expression,
they recapitulate neural tube...
www.cell.com
December 16, 2025 at 1:45 AM
Good to see the peer reviewed version of this published
A chemo-optogenetic system for spatiotemporal control of gene expression
We use it to:
- Reconstruct SHH mediated neural tube patterning in vitro
- Measure extracellular half-life of Shh
www.cell.com/developmenta...
A chemo-optogenetic system for spatiotemporal control of gene expression
We use it to:
- Reconstruct SHH mediated neural tube patterning in vitro
- Measure extracellular half-life of Shh
www.cell.com/developmenta...
Reposted
It’s FINALLY out! 🎉🚀 We have been working on PhotoFiTT for a long time, and it’s now published, introducing a quantitative framework to measure phototoxicity and optimise live-cell microscopy experiments. So excited to finally share the last version! 🔬✨ #PhotoFiTT doi.org/10.1038/s414...
December 15, 2025 at 2:58 PM
It’s FINALLY out! 🎉🚀 We have been working on PhotoFiTT for a long time, and it’s now published, introducing a quantitative framework to measure phototoxicity and optimise live-cell microscopy experiments. So excited to finally share the last version! 🔬✨ #PhotoFiTT doi.org/10.1038/s414...
Reposted
Our latest paper on a novel synthetic condensate platform, LAMA-SPREC, is out in ACS Chemical Biology! It enables chemically switchable and reversible control of protein function via sequestration and release in mammalian cells. 🔬💊🚿
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
A Chemically Switchable Synthetic Condensate Platform for Reversible Protein Sequestration and Release
Creating artificial organelles that sequester and release specific proteins in response to a small molecule in mammalian cells is an attractive approach for regulating protein function. In this work, ...
pubs.acs.org
December 16, 2025 at 5:31 AM
Our latest paper on a novel synthetic condensate platform, LAMA-SPREC, is out in ACS Chemical Biology! It enables chemically switchable and reversible control of protein function via sequestration and release in mammalian cells. 🔬💊🚿
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Reposted
Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session.
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
FEBS Press
RoboMic is an automated confocal microscopy pipeline for high-throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI-driv...
febs.onlinelibrary.wiley.com
December 16, 2025 at 9:53 AM
Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session.
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Reposted
Reposted
🚨New preprint(1/2)! We show that RTK fusion oncoproteins broadly suppress EGFR signaling. How? Sequestration of adapters as the shared principle.
Led by superb PhD student Carol Gao.
www.biorxiv.org/content/10.1...
Implications for drug tolerance/resistance, and includes one big surprise🫧.👇
Led by superb PhD student Carol Gao.
www.biorxiv.org/content/10.1...
Implications for drug tolerance/resistance, and includes one big surprise🫧.👇
December 16, 2025 at 12:05 PM
🚨New preprint(1/2)! We show that RTK fusion oncoproteins broadly suppress EGFR signaling. How? Sequestration of adapters as the shared principle.
Led by superb PhD student Carol Gao.
www.biorxiv.org/content/10.1...
Implications for drug tolerance/resistance, and includes one big surprise🫧.👇
Led by superb PhD student Carol Gao.
www.biorxiv.org/content/10.1...
Implications for drug tolerance/resistance, and includes one big surprise🫧.👇
Reposted
I was invited by @focalplane.bsky.social to write a short description about it:
focalplane.biologists.com/2025/12/12/a...
focalplane.biologists.com/2025/12/12/a...
December 15, 2025 at 8:40 AM
I was invited by @focalplane.bsky.social to write a short description about it:
focalplane.biologists.com/2025/12/12/a...
focalplane.biologists.com/2025/12/12/a...
Reposted
#Mastodon, the Command Center for Large-Scale Lineage-Tracing Microscopy Datasets, is finally heading for publication (well, let's see). To #cite or not to cite shall no longer be the question 🙃, because the #preprint. Software is available in every #Fiji near you.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
December 13, 2025 at 6:14 PM
#Mastodon, the Command Center for Large-Scale Lineage-Tracing Microscopy Datasets, is finally heading for publication (well, let's see). To #cite or not to cite shall no longer be the question 🙃, because the #preprint. Software is available in every #Fiji near you.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted
A reminder to use the right P2A sequence to produce separate proteins - the images are from a GPCR-P2A-mCherry construct using a P2A variant without (upper row) and with (lower row) a preceding GSG linker.
December 12, 2025 at 5:55 PM
A reminder to use the right P2A sequence to produce separate proteins - the images are from a GPCR-P2A-mCherry construct using a P2A variant without (upper row) and with (lower row) a preceding GSG linker.
Reposted
Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Discovering Glycosylation-Dependent Protein Function by Thermal Proteome Profiling
Protein glycosylation regulates essential cellular processes including protein folding, stability, and cell-cell interactions; however, how aberrant glycosylation impacts protein function and interact...
www.biorxiv.org
December 8, 2025 at 2:32 AM
Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted
Pleased to say that Jonas Hartmann (bs-less) and I have finally released DySTrack (“diss track”) - Dynamic Sample Tracking.
It’s a Python-based, modular tool that brings smart microscopy to everyday imaging on commercial systems.
Preprint: www.biorxiv.org/content/10.6...
[1/6]
It’s a Python-based, modular tool that brings smart microscopy to everyday imaging on commercial systems.
Preprint: www.biorxiv.org/content/10.6...
[1/6]
December 8, 2025 at 9:48 AM
Pleased to say that Jonas Hartmann (bs-less) and I have finally released DySTrack (“diss track”) - Dynamic Sample Tracking.
It’s a Python-based, modular tool that brings smart microscopy to everyday imaging on commercial systems.
Preprint: www.biorxiv.org/content/10.6...
[1/6]
It’s a Python-based, modular tool that brings smart microscopy to everyday imaging on commercial systems.
Preprint: www.biorxiv.org/content/10.6...
[1/6]
Reposted
Predicting and Designing Red Fluorescent Protein Variants Using Sequence-to-Function Machine Learning Models by Yufeng Zhao and team: www.biorxiv.org/content/10.6...
December 8, 2025 at 7:09 PM
Predicting and Designing Red Fluorescent Protein Variants Using Sequence-to-Function Machine Learning Models by Yufeng Zhao and team: www.biorxiv.org/content/10.6...
Reposted
🧪I frequently have inexperienced trainees applying a quantitative image analysis pipeline without actually looking at the images to ensure it is valid for the samples.
Case in point: quantifying puncta intensity to monitor protein delocalization.
Case in point: quantifying puncta intensity to monitor protein delocalization.
December 4, 2025 at 6:28 PM
🧪I frequently have inexperienced trainees applying a quantitative image analysis pipeline without actually looking at the images to ensure it is valid for the samples.
Case in point: quantifying puncta intensity to monitor protein delocalization.
Case in point: quantifying puncta intensity to monitor protein delocalization.
Reposted
JASPAR 2026 is out 🎉
The new release massively expands the TF motif collections and adds a dedicated DeepLearning collection of motifs learned from deep learning models.
Database: jaspar.elixir.no
Paper (NAR): doi.org/10.1093/nar/...
🧵1/2
The new release massively expands the TF motif collections and adds a dedicated DeepLearning collection of motifs learned from deep learning models.
Database: jaspar.elixir.no
Paper (NAR): doi.org/10.1093/nar/...
🧵1/2
JASPAR: An open-access database of transcription factor binding profiles
JASPAR is the largest open-access database of curated and non-redundant transcription factor (TF) binding profiles from six different taxonomic groups.
jaspar.elixir.no
December 3, 2025 at 2:43 PM
JASPAR 2026 is out 🎉
The new release massively expands the TF motif collections and adds a dedicated DeepLearning collection of motifs learned from deep learning models.
Database: jaspar.elixir.no
Paper (NAR): doi.org/10.1093/nar/...
🧵1/2
The new release massively expands the TF motif collections and adds a dedicated DeepLearning collection of motifs learned from deep learning models.
Database: jaspar.elixir.no
Paper (NAR): doi.org/10.1093/nar/...
🧵1/2
Reposted
We’ve made some new tools to manipulate N-recognins. Check them out in our preprint.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 29, 2025 at 3:24 AM
We’ve made some new tools to manipulate N-recognins. Check them out in our preprint.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted
Reposted
One of our founding directors Anthony Hyman @hymanlab.bsky.social will become @embl.org Director General: www.mpi-cbg.de/news-outreac...
November 27, 2025 at 1:20 PM
One of our founding directors Anthony Hyman @hymanlab.bsky.social will become @embl.org Director General: www.mpi-cbg.de/news-outreac...
Reposted
It's been great to present OME-Zarrs & Fractal for interoperable bioimage analysis at the 2025 @cytodata.bsky.social conference in Berlin today. Thanks a lot to the organizers & especially Christopher Schmied for having us!
November 20, 2025 at 1:03 PM
It's been great to present OME-Zarrs & Fractal for interoperable bioimage analysis at the 2025 @cytodata.bsky.social conference in Berlin today. Thanks a lot to the organizers & especially Christopher Schmied for having us!
Reposted
CaRPOOL: A Pooled Calcium-Recording CRISPR Screening Platform Identifies CCR7 as a Modulator of Cellular Osmomechanosensing https://www.biorxiv.org/content/10.1101/2025.11.27.690909v1
November 29, 2025 at 3:30 AM
CaRPOOL: A Pooled Calcium-Recording CRISPR Screening Platform Identifies CCR7 as a Modulator of Cellular Osmomechanosensing https://www.biorxiv.org/content/10.1101/2025.11.27.690909v1
Reposted
ロドプシングアニル酸シクラーゼと改変型 cGMP作動性 K⁺ チャネルを用いた神経抑制型オプトジェネティクス🌟
Optogenetic silencing using rhodopsin-GC and engineered cGMP-gated K+ channels🌟
www.science.org/doi/10.1126/...
@johvierock.bsky.social #OpenAccess
Optogenetic silencing using rhodopsin-GC and engineered cGMP-gated K+ channels🌟
www.science.org/doi/10.1126/...
@johvierock.bsky.social #OpenAccess
November 29, 2025 at 7:14 AM
ロドプシングアニル酸シクラーゼと改変型 cGMP作動性 K⁺ チャネルを用いた神経抑制型オプトジェネティクス🌟
Optogenetic silencing using rhodopsin-GC and engineered cGMP-gated K+ channels🌟
www.science.org/doi/10.1126/...
@johvierock.bsky.social #OpenAccess
Optogenetic silencing using rhodopsin-GC and engineered cGMP-gated K+ channels🌟
www.science.org/doi/10.1126/...
@johvierock.bsky.social #OpenAccess
Reposted
🔬New Preprint Alert 🔬 How is AMPK regulated by oxidative stress? We use functional imaging to uncover spatial regulation of AMPK activity during oxidative stress by LKB1 & CaMKK2. Combining imaging with proteomics & metabolomics, we find AMPK works to promote the cellular redox response.
Spatial regulation of AMPK activity under oxidative stress requires LKB1 https://www.biorxiv.org/content/10.1101/2025.11.17.688926v1
November 18, 2025 at 3:56 AM
🔬New Preprint Alert 🔬 How is AMPK regulated by oxidative stress? We use functional imaging to uncover spatial regulation of AMPK activity during oxidative stress by LKB1 & CaMKK2. Combining imaging with proteomics & metabolomics, we find AMPK works to promote the cellular redox response.
Reposted
I enjoyed reading this! www.embopress.org/doi/full/10....
On the role of phase separation in the biogenesis of membraneless compartments | The EMBO Journal
EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.
www.embopress.org
November 14, 2025 at 11:14 AM
I enjoyed reading this! www.embopress.org/doi/full/10....