Jesse Garcia Castillo, PhD
@jgcastillo.bsky.social
CRI Irvington Postdoctoral Fellow/Jeroen Roose’s lab at UCSF. HHMI Gilliam Fellow'21. Berkeley MCB PhD alum/Michel DuPage’s Lab. Cal State LA alum. 🏳️🌈🇲🇽 (He/His/Him). Interested in all things immunology, but T cells are my favorite!
Pinned
Selective disruption of lipid peroxide homeostasis in intratumoral regulatory T cells by targeting FSP1 enhances cancer immunity https://www.biorxiv.org/content/10.1101/2025.07.06.663397v1
My third paper from grad school is out on Biorxiv! This was a fun collaboration with @olzmannlab.bsky.social where we show that Ferroptosis Suppressor Protein-1 is a better target than GPX4 because it disrupts Treg function while leaving effector T cell function intact.
Reposted by Jesse Garcia Castillo, PhD
this is devastating for so many reasons and honestly, shame on the UC. Was hoping to keep the high going after Mamdani's win only come to falling back to earth and register that this country does not want us to succeed. www.insidehighered.com/news/faculty...
UC to Stop Funding Systemwide Postdoc Program
Established in 1984 to encourage women and minority Ph.D.s to pursue academia, the program has attracted right-wing criticism for prioritizing diverse candidates.
www.insidehighered.com
November 5, 2025 at 8:29 PM
this is devastating for so many reasons and honestly, shame on the UC. Was hoping to keep the high going after Mamdani's win only come to falling back to earth and register that this country does not want us to succeed. www.insidehighered.com/news/faculty...
Reposted by Jesse Garcia Castillo, PhD
Ecstatic to share out latest research looking at the role of mitochondrial ABHD11 in controlling T-cell responses. We think this may be a viable target for autoimmunity. Please do give it a read, we would love to hear your thoughts.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Mitochondrial ABHD11 inhibition drives sterol metabolism to modulate T-cell effector function - Nature Communications
α/β-hydrolase domain-containing protein 11 (ABHD11) is a mitochondrial hydrolase, and its expression in CD4 + T-cells has been linked to remission status in rheumatoid arthritis. Here the authors repo...
www.nature.com
November 3, 2025 at 10:20 AM
Ecstatic to share out latest research looking at the role of mitochondrial ABHD11 in controlling T-cell responses. We think this may be a viable target for autoimmunity. Please do give it a read, we would love to hear your thoughts.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Jesse Garcia Castillo, PhD
What we know, 12:10am
[Now in 2 parts]
—Prop 50 wins
—VA Dems flip Gov, LG, AG
—Dems flip ≈13 seats in VA House
—Dems defend NJ-Gov + Assembly
—Dems win NJ+VA trifectas
—Mamdani wins
—PA Dems win supreme court
—ME vote-restricting measure loses
—GA Dems flip 2 statewide offices
—Krasner & Bragg win
[Now in 2 parts]
—Prop 50 wins
—VA Dems flip Gov, LG, AG
—Dems flip ≈13 seats in VA House
—Dems defend NJ-Gov + Assembly
—Dems win NJ+VA trifectas
—Mamdani wins
—PA Dems win supreme court
—ME vote-restricting measure loses
—GA Dems flip 2 statewide offices
—Krasner & Bragg win
November 5, 2025 at 5:10 AM
What we know, 12:10am
[Now in 2 parts]
—Prop 50 wins
—VA Dems flip Gov, LG, AG
—Dems flip ≈13 seats in VA House
—Dems defend NJ-Gov + Assembly
—Dems win NJ+VA trifectas
—Mamdani wins
—PA Dems win supreme court
—ME vote-restricting measure loses
—GA Dems flip 2 statewide offices
—Krasner & Bragg win
[Now in 2 parts]
—Prop 50 wins
—VA Dems flip Gov, LG, AG
—Dems flip ≈13 seats in VA House
—Dems defend NJ-Gov + Assembly
—Dems win NJ+VA trifectas
—Mamdani wins
—PA Dems win supreme court
—ME vote-restricting measure loses
—GA Dems flip 2 statewide offices
—Krasner & Bragg win
Reposted by Jesse Garcia Castillo, PhD
JUST IN: Mamdani, a democratic socialist and state assemblymember, will make history as the first Muslim and South Asian person — as well as the youngest in over a century — to serve as New York City mayor.
Mamdani wins New York City mayoral race, in a historic victory for progressives
Mamdani, a democratic socialist and state assemblymember, will make history as the first Muslim and South Asian person — as well as the youngest in over a century — to serve as New York City mayor.
n.pr
November 5, 2025 at 2:39 AM
JUST IN: Mamdani, a democratic socialist and state assemblymember, will make history as the first Muslim and South Asian person — as well as the youngest in over a century — to serve as New York City mayor.
Reposted by Jesse Garcia Castillo, PhD
A reminder that Zohran won IN SPITE OF his party and not because of it. This matters because while Democrats like Schumer and Jeffries might SAY "Vote Blue No Matter Who" and "Party Unity," they LITERALLY found it easier to go to bat for a guy with a Nazi tat than Mamdani. They will not accept this.
November 5, 2025 at 4:17 AM
A reminder that Zohran won IN SPITE OF his party and not because of it. This matters because while Democrats like Schumer and Jeffries might SAY "Vote Blue No Matter Who" and "Party Unity," they LITERALLY found it easier to go to bat for a guy with a Nazi tat than Mamdani. They will not accept this.
Reposted by Jesse Garcia Castillo, PhD
BREAKING: The Democrats have seized the majority in the Minnesota Senate!
November 5, 2025 at 3:52 AM
BREAKING: The Democrats have seized the majority in the Minnesota Senate!
Reposted by Jesse Garcia Castillo, PhD
Excited to share my postdoc work @olzmannlab.bsky.social! We found lipid droplets, the cell’s lipid storage depots, are subject to oxidative damage and are protected by FSP1. Loss of FSP1 triggers droplet peroxidation and cell death, revealing a new layer of lipid quality control!
shorturl.at/B5XYD
shorturl.at/B5XYD
FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology
Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.
www.nature.com
October 29, 2025 at 8:45 PM
Excited to share my postdoc work @olzmannlab.bsky.social! We found lipid droplets, the cell’s lipid storage depots, are subject to oxidative damage and are protected by FSP1. Loss of FSP1 triggers droplet peroxidation and cell death, revealing a new layer of lipid quality control!
shorturl.at/B5XYD
shorturl.at/B5XYD
Reposted by Jesse Garcia Castillo, PhD
A new #ScienceImmunology study shows that deleting the lysosomal protein Flcn from regulatory T cells can prevent their accumulation in some tissues, thus boosting antitumor immunity in mice. https://scim.ag/4o1g1of
Mitochondrial and lysosomal signaling orchestrates heterogeneous metabolic states of regulatory T cells
Mitochondrial and lysosomal signaling orchestrates Treg cell metabolic states and functional fitness.
scim.ag
October 28, 2025 at 3:40 PM
A new #ScienceImmunology study shows that deleting the lysosomal protein Flcn from regulatory T cells can prevent their accumulation in some tissues, thus boosting antitumor immunity in mice. https://scim.ag/4o1g1of
Reposted by Jesse Garcia Castillo, PhD
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
October 28, 2025 at 8:06 AM
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Reposted by Jesse Garcia Castillo, PhD
Decoding Peripheral Tolerance: TCR Rules for pTreg differentiation in the Gut https://www.biorxiv.org/content/10.1101/2025.10.20.683415v1
October 21, 2025 at 3:16 AM
Decoding Peripheral Tolerance: TCR Rules for pTreg differentiation in the Gut https://www.biorxiv.org/content/10.1101/2025.10.20.683415v1
Reposted by Jesse Garcia Castillo, PhD
@biorxivpreprint.bsky.social The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomic
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 21, 2025 at 1:27 AM
@biorxivpreprint.bsky.social The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomic
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Happy to share this fun collaboration with Rafael Rivera-Lugo and Dan Portnoy. We developed a Listeria strain that induces long-lasting MAIT cell expansion in B6 mice that normally lack MAIT cells with broad anti-bacterial activity and contribute to tumor control independent of CD8+ T cells!
MAIT cells induced by engineered Listeria exhibit antibacterial and antitumor activity https://www.biorxiv.org/content/10.1101/2025.10.13.682223v1
October 15, 2025 at 10:49 AM
Happy to share this fun collaboration with Rafael Rivera-Lugo and Dan Portnoy. We developed a Listeria strain that induces long-lasting MAIT cell expansion in B6 mice that normally lack MAIT cells with broad anti-bacterial activity and contribute to tumor control independent of CD8+ T cells!
Reposted by Jesse Garcia Castillo, PhD
Cleary and Semmrich et al. focused on identifying antibodies that specifically target and deplete tumor-specific Tregs while sparing systemic Tregs (spleen and blood) and effector T cell populations. bit.ly/4pWrzdT @unisouthampton.bsky.social
October 9, 2025 at 11:15 AM
Cleary and Semmrich et al. focused on identifying antibodies that specifically target and deplete tumor-specific Tregs while sparing systemic Tregs (spleen and blood) and effector T cell populations. bit.ly/4pWrzdT @unisouthampton.bsky.social
Reposted by Jesse Garcia Castillo, PhD
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
October 9, 2025 at 9:01 AM
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
Reposted by Jesse Garcia Castillo, PhD
Pre-division TCF1 drop determines long-term CD8 T cell fates @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 7, 2025 at 3:21 PM
Pre-division TCF1 drop determines long-term CD8 T cell fates @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Jesse Garcia Castillo, PhD
Reposted by Jesse Garcia Castillo, PhD
Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function https://www.biorxiv.org/content/10.1101/2025.10.05.680386v1
October 7, 2025 at 12:16 AM
Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function https://www.biorxiv.org/content/10.1101/2025.10.05.680386v1
Reposted by Jesse Garcia Castillo, PhD
📣do not miss out on 7&8 October: Spatial Biology in cancer and immunology research. Register NOW: events.bizzabo.com/SpatialBiology @yefis-immunology.bsky.social @efis-immunology.bsky.social
Spatial Biology: Cancer & Immunology
events.bizzabo.com
October 6, 2025 at 1:41 PM
📣do not miss out on 7&8 October: Spatial Biology in cancer and immunology research. Register NOW: events.bizzabo.com/SpatialBiology @yefis-immunology.bsky.social @efis-immunology.bsky.social
Reposted by Jesse Garcia Castillo, PhD
The impact has been enormous, with Tregs going from being a niche frowned-upon subset of immunology, to underpinning our entire understanding of how the immune system works. Therapies based on boosting Tregs (e.g. IL2) or bypassing Tregs (anti-CTLA4) are in the clinic.
October 6, 2025 at 11:21 AM
The impact has been enormous, with Tregs going from being a niche frowned-upon subset of immunology, to underpinning our entire understanding of how the immune system works. Therapies based on boosting Tregs (e.g. IL2) or bypassing Tregs (anti-CTLA4) are in the clinic.
Great to see Tregs win a Nobel prize!!
A small primer on the #NobelPrize awarded to Mary E. Brunkow, Fred Ramsdell and Shimon Sakaguchi today. This prize was for combining two separate fields of immunology research - genetic research on IPEX and immunology research of regulatory T cells (#Tregs), with enormous impact on biology/medicine
October 6, 2025 at 1:37 PM
Great to see Tregs win a Nobel prize!!
Reposted by Jesse Garcia Castillo, PhD
Renewing my yearly bet on Cooper/Miller for the Nobels.
One might think that I would learn after being wrong so many times, but one would be mistaken.
laskerfoundation.org/winners/b-an...
One might think that I would learn after being wrong so many times, but one would be mistaken.
laskerfoundation.org/winners/b-an...
B and T cells—the organizing principle of the adaptive immune system - Lasker Foundation
For their discovery of the two distinct classes of lymphocytes, B and T cells – a monumental achievement that provided the organizing principle of the adaptive immune system and launched the course of...
laskerfoundation.org
October 4, 2025 at 1:43 PM
Renewing my yearly bet on Cooper/Miller for the Nobels.
One might think that I would learn after being wrong so many times, but one would be mistaken.
laskerfoundation.org/winners/b-an...
One might think that I would learn after being wrong so many times, but one would be mistaken.
laskerfoundation.org/winners/b-an...
Reposted by Jesse Garcia Castillo, PhD
Foreign epitope–specific naïve Treg cells fine-tune the primary response of Tconv cells specific for the same epitope by curbing the Th1 fate while allowing a robust response @jimmunol.bsky.social @uminnpress.bsky.social
academic.oup.com/jimmunol/adv...
academic.oup.com/jimmunol/adv...
October 3, 2025 at 4:00 AM
Foreign epitope–specific naïve Treg cells fine-tune the primary response of Tconv cells specific for the same epitope by curbing the Th1 fate while allowing a robust response @jimmunol.bsky.social @uminnpress.bsky.social
academic.oup.com/jimmunol/adv...
academic.oup.com/jimmunol/adv...
Reposted by Jesse Garcia Castillo, PhD
Eomes controls the acquisition of regulatory and cytotoxic function in CD4+ T cells that are critical for limiting immune toxicity, while maintaining persistence and tumor control after immunotherapy @cp-immunity.bsky.social
www.cell.com/immunity/ful... @fredhutch.org
www.cell.com/immunity/ful... @fredhutch.org
October 2, 2025 at 4:55 PM
Eomes controls the acquisition of regulatory and cytotoxic function in CD4+ T cells that are critical for limiting immune toxicity, while maintaining persistence and tumor control after immunotherapy @cp-immunity.bsky.social
www.cell.com/immunity/ful... @fredhutch.org
www.cell.com/immunity/ful... @fredhutch.org
Reposted by Jesse Garcia Castillo, PhD
Happy to announce the publication using advanced models to understand how T cells mediate efficient immunoediting in early sarcomas.
This was a collaboration between Julie Cheung and Brian Hunt
in the lab
Thanks to Cancer Cell for publishing our work.
www.cell.com/cancer-cell/...
This was a collaboration between Julie Cheung and Brian Hunt
in the lab
Thanks to Cancer Cell for publishing our work.
www.cell.com/cancer-cell/...
Distinct T cell functions enable efficient immunoediting and prevent tumor emergence of developing sarcomas
Cheung et al. describe efficient T cell control of nascent neoantigen+ and nearby
neoantigen-negative sarcoma cells, underlying a ∼50% reduction in tumor penetrance.
This otherwise imperceptible immun...
www.cell.com
October 2, 2025 at 4:50 PM
Happy to announce the publication using advanced models to understand how T cells mediate efficient immunoediting in early sarcomas.
This was a collaboration between Julie Cheung and Brian Hunt
in the lab
Thanks to Cancer Cell for publishing our work.
www.cell.com/cancer-cell/...
This was a collaboration between Julie Cheung and Brian Hunt
in the lab
Thanks to Cancer Cell for publishing our work.
www.cell.com/cancer-cell/...
Reposted by Jesse Garcia Castillo, PhD
Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data www.nature.com/articles/s4...
October 1, 2025 at 2:15 PM
Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data www.nature.com/articles/s4...