Falk Schneider
@faldalf.bsky.social
Assistant Professor at University of Warwick
Leading FMD Lab: https://fmd-lab.org/
Postdoc in Fraser Lab (USC)
Postdoc Fritzsche Lab (Oxford)
PhD in Eggeling Lab (Oxford)
Into all things Biophysics, Fluorescence, and Membranes
https://faldalf.github.io
Leading FMD Lab: https://fmd-lab.org/
Postdoc in Fraser Lab (USC)
Postdoc Fritzsche Lab (Oxford)
PhD in Eggeling Lab (Oxford)
Into all things Biophysics, Fluorescence, and Membranes
https://faldalf.github.io
Pinned
Falk Schneider
@faldalf.bsky.social
· Apr 22
#newPI and time is running.
It has already been a month since I officially joined the University of Warwick as Assistant Professor. I am excited to announce and build the Fluorescence and Membrane Dynamics (FMD) Lab:
warwick.ac.uk/fac/sci/med/...
It has already been a month since I officially joined the University of Warwick as Assistant Professor. I am excited to announce and build the Fluorescence and Membrane Dynamics (FMD) Lab:
warwick.ac.uk/fac/sci/med/...
Reposted by Falk Schneider
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
November 14, 2025 at 8:34 PM
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
Reposted by Falk Schneider
Know the difference.
November 19, 2025 at 10:08 PM
Know the difference.
Reposted by Falk Schneider
Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡
StayRose: A photostable StayGold derivative redshifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence
engineering, addresses thi...
www.jbc.org
November 17, 2025 at 9:03 AM
Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡
Reposted by Falk Schneider
#ZebrafishZunday: Cytoplasmic (or ooplasmic) streaming leads to the segregation of embryo from yolk granules. Credit to @shamipourshayan.bsky.social & @heisenbergcplab.bsky.social. 🧪
November 16, 2025 at 9:31 AM
#ZebrafishZunday: Cytoplasmic (or ooplasmic) streaming leads to the segregation of embryo from yolk granules. Credit to @shamipourshayan.bsky.social & @heisenbergcplab.bsky.social. 🧪
🌹🔬🤩
Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡
StayRose: A photostable StayGold derivative redshifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence
engineering, addresses thi...
www.jbc.org
November 17, 2025 at 9:37 AM
🌹🔬🤩
Reposted by Falk Schneider
Head-mounted 2-photon miniscope voltage imaging!
Weijian Yang's group at UC Davis sped up 2p scanning by multiplexing to achieve 400-Hz voltage imaging of ~20 neurons, using ASAP4e
These are the first freely moving single-cell voltage recordings as far as I know.
URL: www.cell.com/cell-reports...
Weijian Yang's group at UC Davis sped up 2p scanning by multiplexing to achieve 400-Hz voltage imaging of ~20 neurons, using ASAP4e
These are the first freely moving single-cell voltage recordings as far as I know.
URL: www.cell.com/cell-reports...
High-speed neural imaging with multiplexed miniaturized two-photon microscopy
Zhang et al. develop two types of multiplexed miniaturized two-photon microscopes
(M-MINI2Ps) that increase imaging speed via beam multiplexing while preserving spatial
resolution and demonstrate thei...
www.cell.com
November 13, 2025 at 11:33 PM
Head-mounted 2-photon miniscope voltage imaging!
Weijian Yang's group at UC Davis sped up 2p scanning by multiplexing to achieve 400-Hz voltage imaging of ~20 neurons, using ASAP4e
These are the first freely moving single-cell voltage recordings as far as I know.
URL: www.cell.com/cell-reports...
Weijian Yang's group at UC Davis sped up 2p scanning by multiplexing to achieve 400-Hz voltage imaging of ~20 neurons, using ASAP4e
These are the first freely moving single-cell voltage recordings as far as I know.
URL: www.cell.com/cell-reports...
Reposted by Falk Schneider
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Falk Schneider
Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens...
-> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this?
www.fishersci.com/shop/product...
-> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this?
www.fishersci.com/shop/product...
November 12, 2025 at 12:56 AM
Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens...
-> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this?
www.fishersci.com/shop/product...
-> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this?
www.fishersci.com/shop/product...
Reposted by Falk Schneider
Mind-bender: The observable universe has ~10^80 atoms. But even a simple 1000×1000 black‑and‑white image has 2^{1,000,000} ≈ 10^{301,030} possibilities — and 24‑bit color explodes to ~10^{7,224,720}. The space of (small) images dwarfs the cosmos. 🔬🧪💻
November 13, 2025 at 2:31 AM
Mind-bender: The observable universe has ~10^80 atoms. But even a simple 1000×1000 black‑and‑white image has 2^{1,000,000} ≈ 10^{301,030} possibilities — and 24‑bit color explodes to ~10^{7,224,720}. The space of (small) images dwarfs the cosmos. 🔬🧪💻
Reposted by Falk Schneider
Happy to share that I will be starting my independent research career as a Research Group Leader at the German Cancer Research Center (DKFZ). Starting from January 2026, my group will be based at DKFZ National Center for Tumor Diseases (NCT WERA), Würzburg, Germany.
November 11, 2025 at 1:33 PM
Happy to share that I will be starting my independent research career as a Research Group Leader at the German Cancer Research Center (DKFZ). Starting from January 2026, my group will be based at DKFZ National Center for Tumor Diseases (NCT WERA), Würzburg, Germany.
Reposted by Falk Schneider
Get into flow with zebrafish — soon your eyes will glow 🐟✨
Blood flow in a transparent zebrafish embryo. Credit to @damiandn.bsky.social. #ZebrafishZunday 🧪
November 9, 2025 at 12:06 PM
Get into flow with zebrafish — soon your eyes will glow 🐟✨
Reposted by Falk Schneider
Hi Fans of Feedback Microscopy,
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
November 7, 2025 at 7:23 PM
Hi Fans of Feedback Microscopy,
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
Reposted by Falk Schneider
Multiple-Awesome-Colleague Alert! The first is Fred Heberle (not on bsky). His group made a series of 3-component vesicles (high-Tmelt PC-lipid, low-Tmelt PC-lipid and chol) and varied only the high-Tmelt lipid. Then they measured liquid-liquid phase transition temperatures (Tmix) of the membranes 🧵
Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids https://www.biorxiv.org/content/10.1101/2025.10.28.685012v1
November 7, 2025 at 6:16 AM
Multiple-Awesome-Colleague Alert! The first is Fred Heberle (not on bsky). His group made a series of 3-component vesicles (high-Tmelt PC-lipid, low-Tmelt PC-lipid and chol) and varied only the high-Tmelt lipid. Then they measured liquid-liquid phase transition temperatures (Tmix) of the membranes 🧵
Reposted by Falk Schneider
This week, Sarah and I were reflecting how all those years ago, when we were a new PhD student and a new faculty member, we had no idea that our data would be quantitatively relevant and actively used decades later by this awesome community. It’s humbling. www.biophysics.org/blog/a-17-ye...
A 17-Year-Old BJ Article Explored the Ground Rules of Phase Separation in Lipid Bilayer
For Biophysics Week, members of the Publications Committee selected a few influential articles from Biophysical Journal to highlight as well as the people who wrote them. This is the first in the seri...
www.biophysics.org
November 7, 2025 at 6:16 AM
This week, Sarah and I were reflecting how all those years ago, when we were a new PhD student and a new faculty member, we had no idea that our data would be quantitatively relevant and actively used decades later by this awesome community. It’s humbling. www.biophysics.org/blog/a-17-ye...
Reposted by Falk Schneider
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Synthetic fluorophores for live-cell fluorescence microscopy and biosensing - Nature Chemical Biology
Performing live-cell microscopy experiments with high spatial and temporal resolution requires fluorophores with highly optimized properties. This Review examines the progress in developing synthetic ...
www.nature.com
November 5, 2025 at 9:29 PM
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Reposted by Falk Schneider
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
www.fpbase.org
November 4, 2025 at 3:03 PM
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
Reposted by Falk Schneider
I'd only dabbled with napari, but needed to use it for a paper we're writing. This is a quick post about getting started: from installation to a folder full of thousands of processed images, with gotchas along the way.
quantixed.org/2025/11/04/a...
quantixed.org/2025/11/04/a...
Adventures in Code VII: getting started with napari – quantixed
quantixed.org
November 4, 2025 at 10:00 AM
I'd only dabbled with napari, but needed to use it for a paper we're writing. This is a quick post about getting started: from installation to a folder full of thousands of processed images, with gotchas along the way.
quantixed.org/2025/11/04/a...
quantixed.org/2025/11/04/a...
Reposted by Falk Schneider
👇🏻 great opportunity for a PhD, great environment & PI!
🚨 #PhD Opportunities 🚨
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
November 1, 2025 at 8:25 AM
👇🏻 great opportunity for a PhD, great environment & PI!
🚨 #PhD Opportunities 🚨
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
October 31, 2025 at 1:33 PM
🚨 #PhD Opportunities 🚨
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
Reposted by Falk Schneider
Get out your calendars! The motors are out of quarantine and we plan to assemble in person on 8th May 2026 at University of Warwick Campus for a fun day on motors and the filaments they walk along. Livestream is planned too. More details on registration, travel bursaries etc. will be revealed soon.
October 30, 2025 at 2:18 PM
Get out your calendars! The motors are out of quarantine and we plan to assemble in person on 8th May 2026 at University of Warwick Campus for a fun day on motors and the filaments they walk along. Livestream is planned too. More details on registration, travel bursaries etc. will be revealed soon.
Reposted by Falk Schneider
Tracking has always been at the ❤️ of the scientific method, from planetary motion to single particles.
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Tracing fluorescence tracking’s past, present, and future
Artificial intelligence and parallel computing could refine studies of the movement of single molecules inside cells.
www.aip.org
October 30, 2025 at 11:19 PM
Tracking has always been at the ❤️ of the scientific method, from planetary motion to single particles.
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Reposted by Falk Schneider
#ACSChemBio and #Biochemistry invite submissions to a special issue on Lipids and Lipidation guest edited by @chembiobryan.bsky.social and me! Find details in the #CallforPapers and submit your exciting findings to one of these journals by May 31, 2026. axial.acs.org/chemical-bio... #lipidtime
Call for Papers: Lipids and Lipidation | ACS Publications Chemistry Blog
This Special Issue in _ACS Chemical Biology_ will highlight the latest advancements and perspectives in the chemical biology of lipids and lipidation. Submit your manuscript by May 31, 2026.
axial.acs.org
October 28, 2025 at 3:48 PM
#ACSChemBio and #Biochemistry invite submissions to a special issue on Lipids and Lipidation guest edited by @chembiobryan.bsky.social and me! Find details in the #CallforPapers and submit your exciting findings to one of these journals by May 31, 2026. axial.acs.org/chemical-bio... #lipidtime
Reposted by Falk Schneider
Very happy to be in Nashville today for #zebrafishzunday before my talk @vanderbilt.edu tomorrow, so here's a sneak preview of differential clearance of monomeric (turquoise) vs. oligomeric (red) amyloid beta from the zebrafish brain
October 27, 2025 at 2:18 AM
Very happy to be in Nashville today for #zebrafishzunday before my talk @vanderbilt.edu tomorrow, so here's a sneak preview of differential clearance of monomeric (turquoise) vs. oligomeric (red) amyloid beta from the zebrafish brain
Reposted by Falk Schneider
We wrote a paper about this phenomenon! www.molbiolcell.org/doi/full/10..... On stiff substrates, ventral stress fibers deform the plasma membrane. It’s not that GFP colocalizes to actin fibers, but rather high magnification microscopy is able to observe the variation in membrane/cytosol topography.
www.molbiolcell.org
October 24, 2025 at 8:30 PM
We wrote a paper about this phenomenon! www.molbiolcell.org/doi/full/10..... On stiff substrates, ventral stress fibers deform the plasma membrane. It’s not that GFP colocalizes to actin fibers, but rather high magnification microscopy is able to observe the variation in membrane/cytosol topography.
Reposted by Falk Schneider
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....
onlinelibrary.wiley.com
October 25, 2025 at 8:56 AM
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....