Buck O’Flanagan
@buck-oflanagan.bsky.social
5th year PhD student at the University of Minnesota’s Center for Immunology in the Masopust lab. NIH/NIAID F31 scientist investigating how Trm in secondary lymphoid organs orchestrate recall immune responses.
https://scholar.google.com/citations?hl=en&use
https://scholar.google.com/citations?hl=en&use
Reposted by Buck O’Flanagan
Check out the case report we wrote on a fascinating case of teratoma in the testes. This case illustrates that pure mature teratomas occurring in postpubertal males cannot invariably be classified into the benign teratoma category.
www.frontiersin.org/journals/onc...
www.frontiersin.org/journals/onc...
Frontiers | Case Report: Prepubertal-type testicular teratoma with local metastasis in a postpubertal patient
www.frontiersin.org
March 23, 2025 at 11:03 PM
Check out the case report we wrote on a fascinating case of teratoma in the testes. This case illustrates that pure mature teratomas occurring in postpubertal males cannot invariably be classified into the benign teratoma category.
www.frontiersin.org/journals/onc...
www.frontiersin.org/journals/onc...
Reposted by Buck O’Flanagan
Why scratching makes a rash worse and a potential benefit to scratching--it reduced S. aureus on skin. Andrew Liu's paper from our lab is out now at #science #neuroimmune #immunology www.science.org/doi/10.1126/...
Scratching promotes allergic inflammation and host defense via neurogenic mast cell activation
Itch is a dominant symptom in dermatitis, and scratching promotes cutaneous inflammation, thereby worsening disease. However, the mechanisms through which scratching exacerbates inflammation and wheth...
www.science.org
January 30, 2025 at 8:31 PM
Why scratching makes a rash worse and a potential benefit to scratching--it reduced S. aureus on skin. Andrew Liu's paper from our lab is out now at #science #neuroimmune #immunology www.science.org/doi/10.1126/...
Reposted by Buck O’Flanagan
New: Lee et al. @harvardmed.bsky.social provide a compendium of early transcriptional responses to major inflammatory #cytokines across all major immunocyte lineages of the mouse, highlighting unappreciated relationships btwn these cytokines and with other cytokine families https://buff.ly/4gn71Fg
January 28, 2025 at 5:17 PM
New: Lee et al. @harvardmed.bsky.social provide a compendium of early transcriptional responses to major inflammatory #cytokines across all major immunocyte lineages of the mouse, highlighting unappreciated relationships btwn these cytokines and with other cytokine families https://buff.ly/4gn71Fg
Reposted by Buck O’Flanagan
Today in @nature.com. We mapped the spatial and temporal dynamics of intestinal tissue-resident memory CD8 T (TRM) cell differentiation.
🔗 www.nature.com/articles/s41....
@ucsandiego.bsky.social @lji.org @alleninstitute.bsky.social @amonell.bsky.social @reinacampos.bsky.social @GoldrathLab 🧵 1/10
🔗 www.nature.com/articles/s41....
@ucsandiego.bsky.social @lji.org @alleninstitute.bsky.social @amonell.bsky.social @reinacampos.bsky.social @GoldrathLab 🧵 1/10
Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted - Nature
Profiling of the location and transcriptome of tissue-resident memory CD8 T cell formation at single-transcript resolution finds regionalized signalling as the basis of immune diversity in t...
www.nature.com
January 22, 2025 at 4:15 PM
Today in @nature.com. We mapped the spatial and temporal dynamics of intestinal tissue-resident memory CD8 T (TRM) cell differentiation.
🔗 www.nature.com/articles/s41....
@ucsandiego.bsky.social @lji.org @alleninstitute.bsky.social @amonell.bsky.social @reinacampos.bsky.social @GoldrathLab 🧵 1/10
🔗 www.nature.com/articles/s41....
@ucsandiego.bsky.social @lji.org @alleninstitute.bsky.social @amonell.bsky.social @reinacampos.bsky.social @GoldrathLab 🧵 1/10
Reposted by Buck O’Flanagan
Super excited to announce that the Joag Lab will officially open at @UW, @SCRI in March 2025! We will study the interplay between memory T cells and stromal, humoral, and innate immunity in mucosal tissues. researcherprofiles.seattlechildrens.org/Vineet.Joag. Hiring a technician and a postdoc!
January 22, 2025 at 6:14 PM
Super excited to announce that the Joag Lab will officially open at @UW, @SCRI in March 2025! We will study the interplay between memory T cells and stromal, humoral, and innate immunity in mucosal tissues. researcherprofiles.seattlechildrens.org/Vineet.Joag. Hiring a technician and a postdoc!
Reposted by Buck O’Flanagan
New: Amir Ferry, Ananda Goldrath and colleagues @ucsdhealthsci.bsky.social show that differentiation of tissue-resident memory T cells (TRM) is accompanied by the upregulation of the conventional DC1 (cDC1) chemoattractant, Xcl1. https://buff.ly/4hrKCYe
#MucosalImmunology #TumorImmunology
#MucosalImmunology #TumorImmunology
January 22, 2025 at 5:14 PM
New: Amir Ferry, Ananda Goldrath and colleagues @ucsdhealthsci.bsky.social show that differentiation of tissue-resident memory T cells (TRM) is accompanied by the upregulation of the conventional DC1 (cDC1) chemoattractant, Xcl1. https://buff.ly/4hrKCYe
#MucosalImmunology #TumorImmunology
#MucosalImmunology #TumorImmunology
Reposted by Buck O’Flanagan
Excited to share my PhD work from Rafi Ahmed’s lab, now published in @nature.com. We show how CD8 T cells overcome the inability to predict how long a viral infection will last by making adaptable precursor cells a priori—an elegant strategy for a non-prescient immune system! tinyurl.com/53nrpsa6
January 15, 2025 at 10:23 PM
Excited to share my PhD work from Rafi Ahmed’s lab, now published in @nature.com. We show how CD8 T cells overcome the inability to predict how long a viral infection will last by making adaptable precursor cells a priori—an elegant strategy for a non-prescient immune system! tinyurl.com/53nrpsa6
Reposted by Buck O’Flanagan
TCR signaling via NFATc1 constrains IL-15-induced NK-like activation of human memory CD8+ T cells
doi.org/10.1101/2025...
doi.org/10.1101/2025...
January 16, 2025 at 2:10 AM
TCR signaling via NFATc1 constrains IL-15-induced NK-like activation of human memory CD8+ T cells
doi.org/10.1101/2025...
doi.org/10.1101/2025...
Reposted by Buck O’Flanagan
New: Papadopoulos, Kipnis and colleagues present new findings on how lymphatic outflow pathways shape brain health, a critical frontier in brain-immune communication. https://buff.ly/40724ub
January 8, 2025 at 7:40 PM
New: Papadopoulos, Kipnis and colleagues present new findings on how lymphatic outflow pathways shape brain health, a critical frontier in brain-immune communication. https://buff.ly/40724ub
Reposted by Buck O’Flanagan
Elegant work by Jason Cyster's group
"Our study demonstrates that the canonical chemoattractant receptor-ligand systems well-established for lymphocyte homeostatic trafficking to LNs are no longer the major contributors in the inflamed setting." #ImmunoSky
"Our study demonstrates that the canonical chemoattractant receptor-ligand systems well-established for lymphocyte homeostatic trafficking to LNs are no longer the major contributors in the inflamed setting." #ImmunoSky
Inflammation switches the chemoattractant requirements for naive lymphocyte entry into lymph nodes
Lymph node swelling is a well-known symptom of infection. A shift in the chemoattractant code controls lymphocyte recruitment from blood into inflamed lymph nodes, allowing lymphocytes to accumulate so that even rare antigen-specific cells can encounter their activating antigen.
www.cell.com
January 5, 2025 at 4:02 PM
Elegant work by Jason Cyster's group
"Our study demonstrates that the canonical chemoattractant receptor-ligand systems well-established for lymphocyte homeostatic trafficking to LNs are no longer the major contributors in the inflamed setting." #ImmunoSky
"Our study demonstrates that the canonical chemoattractant receptor-ligand systems well-established for lymphocyte homeostatic trafficking to LNs are no longer the major contributors in the inflamed setting." #ImmunoSky
Reposted by Buck O’Flanagan
Here is link to Shixin's new paper: www.science.org/doi/epdf/10....
Nutrient-driven histone code determines exhausted CD8+ T cell fates
ACLY-deficient CAR T cells targeting the tumor-associated antigen disialoganglioside GD2 (GD2 CAR T cells) also resisted acquiring TEX-like properties, exhibiting reduced CD39 expression when co-cultu...
www.science.org
December 14, 2024 at 3:53 PM
Here is link to Shixin's new paper: www.science.org/doi/epdf/10....
Reposted by Buck O’Flanagan
The hypoimmune mouse (MHC-deletion, CD47 overexpression) and all of its organs are completely invisible to allogeneic immune cells
www.cell.com/iscience/ful...
www.cell.com/iscience/ful...
November 29, 2024 at 7:58 PM
The hypoimmune mouse (MHC-deletion, CD47 overexpression) and all of its organs are completely invisible to allogeneic immune cells
www.cell.com/iscience/ful...
www.cell.com/iscience/ful...
Reposted by Buck O’Flanagan
The danger theory of immunity strikes back... rdcu.be/d1uQN
November 26, 2024 at 6:13 PM
The danger theory of immunity strikes back... rdcu.be/d1uQN
Reposted by Buck O’Flanagan
Age-related decline in CD8+ tissue resident memory T cells compromises antitumor immunity
www.nature.com/articles/s43...
www.nature.com/articles/s43...
Age-related decline in CD8+ tissue resident memory T cells compromises antitumor immunity - Nature Aging
Exploring how aging compromises antitumor immunity, the authors reveal an age-related impairment of cytotoxic CD8+ TRM cells in mouse tumor models and clinical samples. They implicate BFAR signaling a...
www.nature.com
November 27, 2024 at 2:26 AM
Age-related decline in CD8+ tissue resident memory T cells compromises antitumor immunity
www.nature.com/articles/s43...
www.nature.com/articles/s43...
Reposted by Buck O’Flanagan
Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!
November 22, 2024 at 11:42 PM
Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!
Reposted by Buck O’Flanagan
Excited for my first bluesky post announcing TWO openings for tenure track positions in virology! I am chair of the search committee and with a new department head Mike Gale we are in an exciting building phase.
Please share with anyone who might be interested: hr.myu.umn.edu/jobs/ext/365...
Please share with anyone who might be interested: hr.myu.umn.edu/jobs/ext/365...
hr.myu.umn.edu
November 22, 2024 at 6:54 PM
Excited for my first bluesky post announcing TWO openings for tenure track positions in virology! I am chair of the search committee and with a new department head Mike Gale we are in an exciting building phase.
Please share with anyone who might be interested: hr.myu.umn.edu/jobs/ext/365...
Please share with anyone who might be interested: hr.myu.umn.edu/jobs/ext/365...
Reposted by Buck O’Flanagan
We’ve jumped on board fully here on 🦋
A starter pack of those working on our favourite cell … the T cell 🥰🙈🥰
If you want an add let us know
go.bsky.app/8B5P25c
A starter pack of those working on our favourite cell … the T cell 🥰🙈🥰
If you want an add let us know
go.bsky.app/8B5P25c
November 19, 2024 at 10:11 PM
We’ve jumped on board fully here on 🦋
A starter pack of those working on our favourite cell … the T cell 🥰🙈🥰
If you want an add let us know
go.bsky.app/8B5P25c
A starter pack of those working on our favourite cell … the T cell 🥰🙈🥰
If you want an add let us know
go.bsky.app/8B5P25c
Retinoic acid and TGF-β – instructors at Trm university?
Interesting stuff from Obers, Evrard, Mackay & al., who delve into environmental cues driving tissue-specific fate decisions of #Trm.
Curious what other site-specific signals guide Trm differentiation in other tissues!
Interesting stuff from Obers, Evrard, Mackay & al., who delve into environmental cues driving tissue-specific fate decisions of #Trm.
Curious what other site-specific signals guide Trm differentiation in other tissues!
Retinoic acid and TGF-β orchestrate organ-specific programs of tissue residency
Tissue-resident memory T (TRM) cells are integral to tissue immunity, persisting in diverse anatomical sites where they adhere to a common transcripti…
www.sciencedirect.com
November 20, 2024 at 2:29 AM
Retinoic acid and TGF-β – instructors at Trm university?
Interesting stuff from Obers, Evrard, Mackay & al., who delve into environmental cues driving tissue-specific fate decisions of #Trm.
Curious what other site-specific signals guide Trm differentiation in other tissues!
Interesting stuff from Obers, Evrard, Mackay & al., who delve into environmental cues driving tissue-specific fate decisions of #Trm.
Curious what other site-specific signals guide Trm differentiation in other tissues!
Reposted by Buck O’Flanagan
Last month in #ScienceImmunology, Alex Chudnovskiy,
@victora.bsky.social, & al. used proximity-based labeling in combination with single-cell transcriptomics to characterize antigen-presenting dendritic cells in tumors and their draining lymph nodes.
@victora.bsky.social, & al. used proximity-based labeling in combination with single-cell transcriptomics to characterize antigen-presenting dendritic cells in tumors and their draining lymph nodes.
Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4+ T cell response
This study characterizes the dendritic cells that present antigen to tumor-specific CD4+ T cells in the tumor and tumor-draining lymph node.
www.science.org
November 19, 2024 at 4:59 PM
Last month in #ScienceImmunology, Alex Chudnovskiy,
@victora.bsky.social, & al. used proximity-based labeling in combination with single-cell transcriptomics to characterize antigen-presenting dendritic cells in tumors and their draining lymph nodes.
@victora.bsky.social, & al. used proximity-based labeling in combination with single-cell transcriptomics to characterize antigen-presenting dendritic cells in tumors and their draining lymph nodes.
Reposted by Buck O’Flanagan
I made a starter pack for those of us interested in or working on T cells. Let me know if you would like to be added or know somebody that should be added.
Looking forward to scientific discussions and updates on our favorite immune cell type!
Looking forward to scientific discussions and updates on our favorite immune cell type!
November 18, 2024 at 5:19 PM
I made a starter pack for those of us interested in or working on T cells. Let me know if you would like to be added or know somebody that should be added.
Looking forward to scientific discussions and updates on our favorite immune cell type!
Looking forward to scientific discussions and updates on our favorite immune cell type!
Reposted by Buck O’Flanagan
go.bsky.app/Eaua5Jk my starter for neuroimmunology
November 19, 2024 at 4:50 AM
go.bsky.app/Eaua5Jk my starter for neuroimmunology
Reposted by Buck O’Flanagan
Ever wondered what T cell immunosurveillance of 🐭 mammary ducts looks like? Check out our image featured on the cover of Science Immunology, showing CD103+ (pink) antiviral memory T cells (cyan) w/ in the ductal epithelium 🤩
November 18, 2024 at 12:04 AM
Ever wondered what T cell immunosurveillance of 🐭 mammary ducts looks like? Check out our image featured on the cover of Science Immunology, showing CD103+ (pink) antiviral memory T cells (cyan) w/ in the ductal epithelium 🤩
How does route of mRNA vaccination impact localization and abundance of memory T cells? shorturl.at/foBMY.
Route of self-amplifying mRNA vaccination modulates the establishment of pulmonary resident memory CD8 and CD4 T cells
Intramuscular mRNA vaccination induces pulmonary resident memory T cells that can be further expanded with intranasal boosting.
shorturl.at
November 18, 2024 at 6:41 PM
How does route of mRNA vaccination impact localization and abundance of memory T cells? shorturl.at/foBMY.