Ryan Langlois
LightNews LightNews
langloislab.bsky.social
Ryan Langlois
@langloislab.bsky.social
450 followers 29 following 3 posts
Professor of Microbiology and Immunology at the University of Minnesota. Interested in virus-host interactions, antiviral immunity, and virus evolution.
Posts Replies Media Videos
Reposted by Ryan Langlois
plosbiology.org
What are the #antiviral barriers to cross-species transmission of #zoonotic #influenza A viruses? @jordanbeckerphd.bsky.social @langloislab.bsky.social &co show that CpG-enriched avian #viruses are restricted by several mammalian proteins, including ZAP & KHNYN @plosbiology.org 🧪 plos.io/47fkMVp
Prenylated human ZAP and mammalian KHNYN can independently restrict naturally CpG-enriched ROSV. Top: CpG content of retroviruses (ROSV in black, ROSV-mCherry reporter virus in pink dashed line, MLV in orange, and HIV-1 in blue) calculated as # CpG per 100 nucleotides over a sliding window (graph). Schematic of retrovirus open reading frame (ORF) organization (top). Bottom left: Retroviral assembly and single-cycle infectivity results of human ZAP-L, ZAP-S, and mutants effects on ROSV showing relative percent ROSV infected cells (% mCherry-positive; % mCh+) as determined by flow cytometry. DF-1 cells transfected with increasing plasmid amounts of indicated host proteins (100, 200, or 400 ng), 1,500 ng ROSV, and 200 ng VSV-G. Infectivity is relative to backbone pcDNA3.2 transfections (Mock). Below, western blots detecting ROSV cleaved Gagp19 in supernatant virions as well as full-length ROSV GagPr76, ZAP proteins, and ACTIN loading controls from cellular lysates. Bottom right: Retroviral assembly and single-cycle infectivity results of human KHNYN, mutants, and mammalian KHNYN homologs (from pig, dog, and platypus) on ROSV infectivity. Below, western blots detecting ROSV cleaved Gagp19 in supernatant virions as well as full-length ROSV GagPr76, human KHNYN proteins, and ACTIN loading controls from cellular lysates. Note mammalian KHNYN orthologues were not detected by human KHNYN antibodies.
October 29, 2025 at 5:31 PM
langloislab.bsky.social
Excited to announce that we have a new faculty search opening in the Department of Microbiology and Immunology at the University of Minnesota for a tenure track Assistant Professor in RNA Virology. More info can be found here: hr.myu.umn.edu/jobs/ext/365...
Careers
Minimum qualifications:PhD, MD, or equivalent in a relevant field of study, plus applicable postdoctoral experience.
hr.myu.umn.edu
October 26, 2025 at 2:41 PM
Reposted by Ryan Langlois
christopherbrooke.bsky.social
@joelrc.bsky.social 's paper on differences between the seasonal H1N1 and H3N2 lineages in their relative capacity to antagonize IFN induction and signaling at the single cell level is out now in PLOS Pathogens:

journals.plos.org/plospathogen...
Seasonal influenza a virus lineages exhibit divergent abilities to antagonize interferon induction and signaling
Author summary Successful replication and transmission of influenza A viruses (IAVs) requires effective subversion of the innate immune response. We developed a new single cell method to examine the i...
journals.plos.org
December 12, 2024 at 6:58 PM
langloislab.bsky.social
Excited for my first bluesky post announcing TWO openings for tenure track positions in virology! I am chair of the search committee and with a new department head Mike Gale we are in an exciting building phase.

Please share with anyone who might be interested: hr.myu.umn.edu/jobs/ext/365...
hr.myu.umn.edu
November 22, 2024 at 6:54 PM