Anoop Chauhan
@anoopsinghchauhan.bsky.social
Cancer Biologist. Unraveling the secrets of genomic stability and its disruption.
University of Birmingham, UK
University of Birmingham, UK
Pinned
PIN1-SUMO2/3 motif suppresses excessive RNF168 chromatin accumulation and ubiquitin signaling to promote IR resistance - Nature Communications
The ubiquitin ligase, RNF168, promotes DNA break repair but must be regulated to prevent run-away ubiquitin signaling. Here, the authors identify a three-step post-translational cascade regulating RNF...
www.nature.com
📢 Just published! Excited to share my latest research, now published in Nature Communications! Grateful to my collaborators and mentors who made this work possible.
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
Reposted by Anoop Chauhan
Interested in inherited chromosomal instability syndromes and DNA repair? We are looking for an enthusiastic person to join the Stewart lab at the University of Birmingham as a senior technician. Please checkout the job advert: www.jobs.ac.uk/job/DPH515/senior-research-technician
Senior Research Technician at University of Birmingham
An academic position as a Senior Research Technician is being advertised on jobs.ac.uk. Click now to find more details and explore additional academic job opportunities.
www.jobs.ac.uk
November 6, 2025 at 8:34 AM
Interested in inherited chromosomal instability syndromes and DNA repair? We are looking for an enthusiastic person to join the Stewart lab at the University of Birmingham as a senior technician. Please checkout the job advert: www.jobs.ac.uk/job/DPH515/senior-research-technician
Fascinating work from @raychaudhurilab.bsky.social and @cejkalab.bsky.social! Challenges the idea that HR defects in BRCA2-deficient cells stem from poor RAD51 loading, shows instead that unchecked RAD51 removal by FIGNL1 drives the defect. Great read!
A tour de force by Raviprasad Kuthethur and amazing collaborations from @cejkalab.bsky.social ,Shyam Sharan and Krishna Mohan Poluri labs. Thanks a lot to Safa Nasrin, Satheesh Kumar Sengodan, Stefan Braunshier, Carmen Fonseca , Nupur Nagar, Ananya Acharya for putting it all together.
November 5, 2025 at 7:32 PM
Fascinating work from @raychaudhurilab.bsky.social and @cejkalab.bsky.social! Challenges the idea that HR defects in BRCA2-deficient cells stem from poor RAD51 loading, shows instead that unchecked RAD51 removal by FIGNL1 drives the defect. Great read!
Reposted by Anoop Chauhan
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
ICYMI: New Online! Decoding ubiquitin signals inside cells
Decoding ubiquitin signals inside cells
Nature Reviews Molecular Cell Biology, Published online: 31 October 2025; doi:10.1038/s41580-025-00919-zIn this Tools of the Trade article, Kiss (Schulman lab) discusses the development of UbiREAD, a method that delivers in vitro-ubiquitinated protein reporters into cells to systematically assess how different ubiquitin chain configurations affect protein stability and degradation.
bit.ly
November 4, 2025 at 10:04 AM
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Reposted by Anoop Chauhan
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Reposted by Anoop Chauhan
Required reading for cell biologists to get a sense of basic statistical principles!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Ten essential tips for robust statistics in cell biology - Nature Cell Biology
Statistical thinking is a core part of solid, trustworthy biology. However, many studies still include insufficient sample sizes, have poor experimental design or select an incorrect statistical metho...
www.nature.com
November 4, 2025 at 7:23 PM
Required reading for cell biologists to get a sense of basic statistical principles!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Anoop Chauhan
SUMO interactions: the 1st of two reviews which Aanchal (@origichals.bsky.social), El Hadji, and I have recently written on non-covalent interactions of SUMO is now out. The 1st covers interactions with E1, E2, and deSUMOylating enzymes; the 2nd - on interactions with SIMs etc. - will appear soon.
Non-covalent SUMO interactions with (de)conjugation enzymes
SUMOylation – a protein post-translational modification (PTM) related to ubiquitylation – involves the reversible covalent attachment of the small ubiquitin-like modifier (SUMO) to proteins. During th...
portlandpress.com
November 5, 2025 at 1:44 PM
SUMO interactions: the 1st of two reviews which Aanchal (@origichals.bsky.social), El Hadji, and I have recently written on non-covalent interactions of SUMO is now out. The 1st covers interactions with E1, E2, and deSUMOylating enzymes; the 2nd - on interactions with SIMs etc. - will appear soon.
Reposted by Anoop Chauhan
Excited to share our latest publication, out in @science.org! We define a key role for the CST complex in double-strand break repair pathway choice. This study was conducted in close collaboration with the Sung lab at UT Health San Antonio. Congrats to @michelleswift.bsky.social and all authors!
CTC1-STN1-TEN1 controls DNA break repair pathway choice via DNA end resection blockade
Antagonistic activities of the 53BP1 axis and the tumor suppressor BRCA1-BARD1 determine whether DNA double-strand breaks (DSBs) are repaired by end joining or homologous recombination. We show that t...
www.science.org
May 22, 2025 at 9:30 PM
Excited to share our latest publication, out in @science.org! We define a key role for the CST complex in double-strand break repair pathway choice. This study was conducted in close collaboration with the Sung lab at UT Health San Antonio. Congrats to @michelleswift.bsky.social and all authors!
Reposted by Anoop Chauhan
Proud to present our work identifying a role for DIAPH1 and gamma-actin in regulating DSB repair and how defects in this pathway give rise to human disease. Big thanks to everyone involved, especially Beth Woodward, Sudipta Lahiri and @anoopsinghchauhan.bsky.social. www.nature.com/articles/s41...
Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin - Nature Communications
DNA double strand break repair pathways ensure genome stability and prevent disease. Here the authors show that the actin nucleating factor DIAPH1 and γ-actin promote homologous recombination (HR)-dep...
www.nature.com
May 14, 2025 at 7:59 PM
Proud to present our work identifying a role for DIAPH1 and gamma-actin in regulating DSB repair and how defects in this pathway give rise to human disease. Big thanks to everyone involved, especially Beth Woodward, Sudipta Lahiri and @anoopsinghchauhan.bsky.social. www.nature.com/articles/s41...
Reposted by Anoop Chauhan
Thrilled to see this “veritable tour de force” led by John_C_Fielden from Jacob Corn lab out @nature.com, rdcu.be/ehd4E. This @erc.europa.eu funded project uses #CRISPRi to systematically scan 548 DDR genes for synthetic lethal interactions, ~150,000 pairwise combinations!
Comprehensive interrogation of synthetic lethality in the DNA damage response
Nature - Combinatorial CRISPRi screening was used to map genetic interactions in DNA damage response pathways, revealing known and new connections, including the roles of WDR48 and USP1 in...
rdcu.be
April 14, 2025 at 10:22 AM
Thrilled to see this “veritable tour de force” led by John_C_Fielden from Jacob Corn lab out @nature.com, rdcu.be/ehd4E. This @erc.europa.eu funded project uses #CRISPRi to systematically scan 548 DDR genes for synthetic lethal interactions, ~150,000 pairwise combinations!
📢 Just published! Excited to share my latest research, now published in Nature Communications! Grateful to my collaborators and mentors who made this work possible.
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
PIN1-SUMO2/3 motif suppresses excessive RNF168 chromatin accumulation and ubiquitin signaling to promote IR resistance - Nature Communications
The ubiquitin ligase, RNF168, promotes DNA break repair but must be regulated to prevent run-away ubiquitin signaling. Here, the authors identify a three-step post-translational cascade regulating RNF...
www.nature.com
April 14, 2025 at 1:17 PM
📢 Just published! Excited to share my latest research, now published in Nature Communications! Grateful to my collaborators and mentors who made this work possible.
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
Reposted by Anoop Chauhan
We are looking for a senior administrator to take care of the Structural Studies Division at @mrclmb.bsky.social. Come and work for the benefit of human health in a fantastic environment. Happy to chat!
www.jobs.ac.uk/job/DLY086/s...
www.jobs.ac.uk/job/DLY086/s...
Senior Administrator (Divisional Administrator) - Structural Studies - LMB 2565 at MRC Laboratory of Molecular Biology
Start your UK & international job search for academic jobs, research jobs, science jobs and managerial jobs in leading universities and top...
www.jobs.ac.uk
February 19, 2025 at 2:27 PM
We are looking for a senior administrator to take care of the Structural Studies Division at @mrclmb.bsky.social. Come and work for the benefit of human health in a fantastic environment. Happy to chat!
www.jobs.ac.uk/job/DLY086/s...
www.jobs.ac.uk/job/DLY086/s...
Reposted by Anoop Chauhan
Join the first Keystone conference dedicated to DNA replication gaps and cancer! Send an abstract to be selected for a talk.
www.keystonesymposia.org/conferences/...
www.keystonesymposia.org/conferences/...
DNA Replication Gaps, Cancer and Disease | Keystone Symposia
Join us at the Keystone Symposia on DNA Replication Gaps, Cancer and Disease, April 2025, in Daejeon, with field leaders!
www.keystonesymposia.org
January 17, 2025 at 11:35 AM
Join the first Keystone conference dedicated to DNA replication gaps and cancer! Send an abstract to be selected for a talk.
www.keystonesymposia.org/conferences/...
www.keystonesymposia.org/conferences/...
Reposted by Anoop Chauhan
Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Cell cycle progression of under-replicated cells
Abstract. Cell cycle checkpoints are the regulatory mechanisms that secure the strict order of cellular events for cell division that ensure genome integri
academic.oup.com
January 10, 2025 at 5:42 PM
Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Reposted by Anoop Chauhan
Excited to see the final published form of this amazing @drewberry.bsky.social animation of DNA repair go live! Watch your favourite friends BRCA1/2, RPA, RAD51, Exo1, BLM, PCNA, MRN, primase and DNA polymerase perform their recombination magic. Cool educational & scientific tool for our community.
Delighted to publish my new molecular animation:
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
DNA Break Repair by Homologous Recombination (2024) Drew Berry wehi.tv
YouTube video by WEHImovies
youtu.be
December 4, 2024 at 1:46 AM
Excited to see the final published form of this amazing @drewberry.bsky.social animation of DNA repair go live! Watch your favourite friends BRCA1/2, RPA, RAD51, Exo1, BLM, PCNA, MRN, primase and DNA polymerase perform their recombination magic. Cool educational & scientific tool for our community.
Reposted by Anoop Chauhan
DNA nicks in either the leading or lagging strand templates can trigger break-induced replication (although nicks are introduced with Cas9n, Flp or gp2 that also create an R-loop or covalent protein barrier). In yeast. www.cell.com/molecular-ce...
DNA nicks in both leading and lagging strand templates can trigger break-induced replication
Xu et al. demonstrate that single-stranded breaks in either the leading or lagging
template DNA strand can initiate break-induced replication in fission yeast. Various
nicking enzymes, including Cas9n...
www.cell.com
December 3, 2024 at 8:21 PM
DNA nicks in either the leading or lagging strand templates can trigger break-induced replication (although nicks are introduced with Cas9n, Flp or gp2 that also create an R-loop or covalent protein barrier). In yeast. www.cell.com/molecular-ce...
Reposted by Anoop Chauhan
Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition 🧪
Congrats @richpossemato.bsky.social & team for the very cool work!
www.nature.com/articles/s42...
Congrats @richpossemato.bsky.social & team for the very cool work!
www.nature.com/articles/s42...
Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition - Nature Metabolism
Nam et al. show that limited glucose availability similar to the tumour microenvironment confers resistance against chemotherapeutic drugs that target DNA synthesis.
www.nature.com
November 27, 2024 at 5:22 AM
Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition 🧪
Congrats @richpossemato.bsky.social & team for the very cool work!
www.nature.com/articles/s42...
Congrats @richpossemato.bsky.social & team for the very cool work!
www.nature.com/articles/s42...
My first post on BlueSky.
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
E3 ligases: a ubiquitous link between DNA repair, DNA replication and human disease
Maintenance of genome stability is of paramount importance for the survival of an organism. However, genomic integrity is constantly being challenged by various endogenous and exogenous processes that...
portlandpress.com
November 18, 2024 at 7:26 AM
My first post on BlueSky.
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Our latest review delves into the critical roles of ubiquitin E3 ligases in safeguarding genome integrity and their implications in human diseases.
@drjhujh.bsky.social @profstewartlab.bsky.social
portlandpress.com/biochemj/art...
Reposted by Anoop Chauhan
Hi BlueSky! It's great to be here. Here's a handy Starter Pack of DNA repair & genome stability groups that I found so far go.bsky.app/NvFWKvL
November 13, 2024 at 9:03 AM
Hi BlueSky! It's great to be here. Here's a handy Starter Pack of DNA repair & genome stability groups that I found so far go.bsky.app/NvFWKvL