Santosh Phuyal
@sphuyal.bsky.social
Scientist @MalmbergLab investigating molecules that regulate interaction between immune cells and cancer cells. 🧪🧬🔬
Reposted by Santosh Phuyal
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
September 10, 2025 at 7:18 AM
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
Reposted by Santosh Phuyal
Our review on the role of ER proteostasis in bone disease is out today in Trends in Molecular Medicine www.cell.com/trends/molec...
The endoplasmic reticulum proteostasis network and bone disease
Bone homeostasis relies on the coordinated activity of osteoblasts and osteoclasts
that balances bone formation and resorption, and of osteocytes for biomechanical sensing
and hormone secretion. A key...
www.cell.com
July 11, 2025 at 9:22 AM
Our review on the role of ER proteostasis in bone disease is out today in Trends in Molecular Medicine www.cell.com/trends/molec...
And the current publishing akd reviewing system
Science is fun, but
Academia.. needs work
Academia.. needs work
August 22, 2025 at 5:09 PM
And the current publishing akd reviewing system
Reposted by Santosh Phuyal
Beautiful mural honoring Henrietta Lacks painted by Baltimore community members popped up near Hopkins med campus recently.
August 17, 2025 at 1:40 PM
Beautiful mural honoring Henrietta Lacks painted by Baltimore community members popped up near Hopkins med campus recently.
Reposted by Santosh Phuyal
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9 #protocol #starprotocols #cellpress
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which streamlines N/C-terminal tagging using an antibiotic resistance cassette for rapid selection and enrichment of gene-edited cells. We describe in detail guide RNA and HDR donor plasmid cloning, transfection of editin...
dlvr.it
August 13, 2025 at 10:22 AM
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Reposted by Santosh Phuyal
Interested in lysosome damage and repair pathways? Check out the latest updates.
New Online! Lysosomal membrane homeostasis and its importance in physiology and disease
Lysosomal membrane homeostasis and its importance in physiology and disease
Nature Reviews Molecular Cell Biology, Published online: 04 August 2025; doi:10.1038/s41580-025-00873-wLysosomes degrade cellular components, and their membrane is an important signalling hub. Recent insights into the mechanisms that maintain lysosomal membrane homeostasis — including the interplay between membrane damage, repair, lysophagy and lysosome biogenesis — highlight their importance in physiology, in disease and during ageing.
bit.ly
August 5, 2025 at 1:02 PM
Interested in lysosome damage and repair pathways? Check out the latest updates.
Reposted by Santosh Phuyal
Check out our lab's latest paper. Laura Downie found that the Lamin B Receptor (LBR) can be used to inducibly label all kinds of membrane contact sites in live cells
doi.org/10.1371/jour...
doi.org/10.1371/jour...
Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor
ER-membrane contact sites are often distorted by existing labeling methods. This study develops an inducible labeling system called LaBeRing that uses the Lamin B receptor to fluorescently tag a varie...
doi.org
July 14, 2025 at 10:58 AM
Check out our lab's latest paper. Laura Downie found that the Lamin B Receptor (LBR) can be used to inducibly label all kinds of membrane contact sites in live cells
doi.org/10.1371/jour...
doi.org/10.1371/jour...
Reposted by Santosh Phuyal
Our new paper is out in Leukemia on Targeting proteostasis in multiple myeloma through inhibition of LTK. Thanks to Ludvig and Thea for this great collaborative work
rdcu.be/evyfX
rdcu.be/evyfX
Targeting proteostasis in multiple myeloma through inhibition of LTK
Leukemia - Targeting proteostasis in multiple myeloma through inhibition of LTK
rdcu.be
July 9, 2025 at 5:55 PM
Our new paper is out in Leukemia on Targeting proteostasis in multiple myeloma through inhibition of LTK. Thanks to Ludvig and Thea for this great collaborative work
rdcu.be/evyfX
rdcu.be/evyfX
Reposted by Santosh Phuyal
Molecular force measurements in vivo are notoriously difficult. We present a new class of programmable force sensors based on engineered coiled-coils, with modular readouts, including fluorescence and luminescence. doi.org/10.1101/2025...
Genetically encoded mechano-sensors with versatile readouts and compact size
Mechanical forces are critical for virtually all fundamental biological processes, yet quantification of mechanical forces at the molecular scale in vivo remains challenging. Here, we present a new st...
doi.org
January 20, 2025 at 9:41 PM
Molecular force measurements in vivo are notoriously difficult. We present a new class of programmable force sensors based on engineered coiled-coils, with modular readouts, including fluorescence and luminescence. doi.org/10.1101/2025...
It is that time of the year 🎄🎅
December 17, 2024 at 11:03 AM
It is that time of the year 🎄🎅
Just finished reading The Mercy of Gods, and what a great book it is! 📚 Can't wait for the other books in the series
December 11, 2024 at 9:08 PM
Just finished reading The Mercy of Gods, and what a great book it is! 📚 Can't wait for the other books in the series
Reposted by Santosh Phuyal
This is pretty wild! Efficient non-viral genome editing of immune cells using circular single-stranded DNA. #science #hemesky 🩸🔬 www.nature.com/articles/s41...
Efficient non-viral immune cell engineering using circular single-stranded DNA-mediated genomic integration - Nature Biotechnology
CAR-T and NK cells engineered using a non-viral circular single-stranded DNA system show effective anti-tumor function.
www.nature.com
December 11, 2024 at 7:03 PM
This is pretty wild! Efficient non-viral genome editing of immune cells using circular single-stranded DNA. #science #hemesky 🩸🔬 www.nature.com/articles/s41...
Today, I discovered that google's NotebookLM generates quite nice podcasts based on pdf files of scientific research articles with great accuracy. Pretty neat #AI tool.
December 5, 2024 at 6:13 PM
Today, I discovered that google's NotebookLM generates quite nice podcasts based on pdf files of scientific research articles with great accuracy. Pretty neat #AI tool.
Reposted by Santosh Phuyal
Perfect reason for my first post here. Super excited to see this work on PIEZO mechanosensing of the intestinal stem cell niche led by Meryem Baghdadi @vignjeviclab.bsky.social and Kim lab out in Science! Honored to have contributed to this story as 2nd author. 🎉
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
PIEZO-dependent mechanosensing is essential for intestinal stem cell fate decision and maintenance
Stem cells perceive and respond to biochemical and physical signals to maintain homeostasis. Yet, it remains unclear how stem cells sense mechanical signals from their niche in vivo. In this work, we ...
www.science.org
December 1, 2024 at 7:17 PM
Perfect reason for my first post here. Super excited to see this work on PIEZO mechanosensing of the intestinal stem cell niche led by Meryem Baghdadi @vignjeviclab.bsky.social and Kim lab out in Science! Honored to have contributed to this story as 2nd author. 🎉
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Santosh Phuyal
Mechano-dependent sorbitol accumulation supports biomolecular condensate
🧪
www.cell.com/cell/abstrac...
🧪
www.cell.com/cell/abstrac...
Mechano-dependent sorbitol accumulation supports biomolecular condensate
Torrino et al. identify the mechanosensitive polyol pathway as a natural mediator
of cytoplasmic and nuclear protein condensates through tightly controlled intracellular
sorbitol accumulation. Extrace...
www.cell.com
December 1, 2024 at 6:28 AM
Mechano-dependent sorbitol accumulation supports biomolecular condensate
🧪
www.cell.com/cell/abstrac...
🧪
www.cell.com/cell/abstrac...
Reposted by Santosh Phuyal
Cancer-associated fibroblasts maintain critical pancreatic cancer cell lipid homeostasis in the tumor microenvironment www.cell.com/cell-reports...
Cancer-associated fibroblasts maintain critical pancreatic cancer cell lipid homeostasis in the tumor microenvironment
Han et al. identify crosstalk between CAFs and pancreatic cancer cells under hypoxia
and nutrient scarcity. Lysophosphatidylcholines (LPCs), as the key mediators, can
be utilized for phosphatidylcholi...
www.cell.com
November 26, 2024 at 5:54 PM
Cancer-associated fibroblasts maintain critical pancreatic cancer cell lipid homeostasis in the tumor microenvironment www.cell.com/cell-reports...
Reposted by Santosh Phuyal
🎨 In case anyone need…
The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!
Check it out 👇
bioart.niaid.nih.gov
The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!
Check it out 👇
bioart.niaid.nih.gov
November 23, 2024 at 3:50 PM
🎨 In case anyone need…
The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!
Check it out 👇
bioart.niaid.nih.gov
The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!
Check it out 👇
bioart.niaid.nih.gov
Reposted by Santosh Phuyal
Beautiful paper from Burkhard Ludewig’s group showing the importance of immunoregulatory stroma to support anti-tumour immunity.
www.cell.com/action/showP...
www.cell.com/action/showP...
www.cell.com
November 19, 2024 at 8:50 PM
Beautiful paper from Burkhard Ludewig’s group showing the importance of immunoregulatory stroma to support anti-tumour immunity.
www.cell.com/action/showP...
www.cell.com/action/showP...
Reposted by Santosh Phuyal
Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib
November 17, 2024 at 4:04 PM
Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib