Sam Lord
@samjlord.bsky.social
Cell biology and microscopy researcher in the @MullinsLab at UCSF. I publish as Samuel J. Lord. https://blog.everydayscientist.com/
Pinned
Sam Lord
@samjlord.bsky.social
· Nov 18
Q: What's wrong with these plots?
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
Update: our #PeerReplication idea is now published in EMBO Reports: doi.org/10.1038/s443...
February 9, 2026 at 7:02 PM
Update: our #PeerReplication idea is now published in EMBO Reports: doi.org/10.1038/s443...
Reposted by Sam Lord
#arxiv #April1st PromoPlot: Covering open-access fees by filling wasted space in corner plots https://arxiv.org/abs/2503.24254 a novel technique can allow authors to reduce or eliminate publication charges for journals such as MNRAS and ApJ.
April 1, 2025 at 8:35 AM
#arxiv #April1st PromoPlot: Covering open-access fees by filling wasted space in corner plots https://arxiv.org/abs/2503.24254 a novel technique can allow authors to reduce or eliminate publication charges for journals such as MNRAS and ApJ.
Here's the reason I ask: I'd like to write up about some basic science researchers who preregistered their plan and how the experience ultimately played out. #OpenScience #Preregistration
blog.everydayscientist.com/the-case-for...
blog.everydayscientist.com/the-case-for...
January 24, 2026 at 1:55 AM
Here's the reason I ask: I'd like to write up about some basic science researchers who preregistered their plan and how the experience ultimately played out. #OpenScience #Preregistration
blog.everydayscientist.com/the-case-for...
blog.everydayscientist.com/the-case-for...
Any cell biologists out there who have preregistered your data collection/analysis plans before starting an experiment? Either on an official depository or unofficially? I'd be curious about your experience.
January 21, 2026 at 11:48 PM
Any cell biologists out there who have preregistered your data collection/analysis plans before starting an experiment? Either on an official depository or unofficially? I'd be curious about your experience.
Here's my idea for better science funding: blog.everydayscientist.com/end-grant-wr...
Instead of funding grants, just award financial prizes for good papers. Award half the prize upon publication; award the second half upon replication, 25% to the original authors and 25% to the replicators.
Instead of funding grants, just award financial prizes for good papers. Award half the prize upon publication; award the second half upon replication, 25% to the original authors and 25% to the replicators.
Everyday Scientist » end grant writing; award prizes for papers instead
blog.everydayscientist.com
January 21, 2026 at 12:57 AM
Here's my idea for better science funding: blog.everydayscientist.com/end-grant-wr...
Instead of funding grants, just award financial prizes for good papers. Award half the prize upon publication; award the second half upon replication, 25% to the original authors and 25% to the replicators.
Instead of funding grants, just award financial prizes for good papers. Award half the prize upon publication; award the second half upon replication, 25% to the original authors and 25% to the replicators.
Reposted by Sam Lord
Preprint drop! 🚨 Rhobin-tag your proteins and watch Saci glow at high temps! 🔥 Bright, photostable, live-cell imaging made possible with JF dyes. Huge thanks to @samjlord.bsky.social @acharlesorszag.bsky.social @mullinslab.bsky.social and Bo Huang lab at UCSF.😊
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy
De novo protein design has emerged as a powerful strategy with the promise to create new tools. The practical performance of designed fluorophore binders, however, has remained far from meeting fluore...
www.biorxiv.org
June 25, 2025 at 5:57 PM
Preprint drop! 🚨 Rhobin-tag your proteins and watch Saci glow at high temps! 🔥 Bright, photostable, live-cell imaging made possible with JF dyes. Huge thanks to @samjlord.bsky.social @acharlesorszag.bsky.social @mullinslab.bsky.social and Bo Huang lab at UCSF.😊
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sam Lord
So grateful to all collaborators on this huge effort!
@mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
@mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
November 25, 2025 at 8:41 PM
So grateful to all collaborators on this huge effort!
@mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
@mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
Reposted by Sam Lord
The Incindiamoeba preprint is out and getting great press! (featuring one of Natalie Petek's HOTTT movies) Congratulations to Beryl Rappaport (@hbrappap.bsky.social) and everyone else who made this happen (including @samjlord.bsky.social and @skruberk.bsky.social). Super-fun collaboration!
‘Fire amoeba’ survives in hotter conditions than any other complex cell
The single-celled organism can grow at 63 °C, a record for eukaryotic life.
www.nature.com
December 2, 2025 at 5:47 PM
The Incindiamoeba preprint is out and getting great press! (featuring one of Natalie Petek's HOTTT movies) Congratulations to Beryl Rappaport (@hbrappap.bsky.social) and everyone else who made this happen (including @samjlord.bsky.social and @skruberk.bsky.social). Super-fun collaboration!
Reposted by Sam Lord
Preprint alert! I’m publishing my last paper from my postdoc work on archaeal cell biology, with @mullinslab.bsky.social, @archaellum.bsky.social, @samjlord.bsky.social, @marleenvw.bsky.social, @arghya93.bsky.social, and more great folks (thread below with more details)
April 16, 2025 at 6:55 PM
Preprint alert! I’m publishing my last paper from my postdoc work on archaeal cell biology, with @mullinslab.bsky.social, @archaellum.bsky.social, @samjlord.bsky.social, @marleenvw.bsky.social, @arghya93.bsky.social, and more great folks (thread below with more details)
Reposted by Sam Lord
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 6, 2025 at 5:58 PM
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sam Lord
Calling all Bay Area researchers for a paid opportunity: the @asterainstitute.bsky.social Open Science Program’s User Testing Day in Emeryville on Feb 11!
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
Application to the Astera Open Science program's user testing day
Are you a Bay Area researcher in academia or industry? Do you want to make an impact on innovative tools designed to transform the way we work and think? Apply below to be a part of the Astera Open Sc...
forms.gle
January 27, 2025 at 7:56 PM
Calling all Bay Area researchers for a paid opportunity: the @asterainstitute.bsky.social Open Science Program’s User Testing Day in Emeryville on Feb 11!
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
📍 Try cutting-edge research tools
💡 Share feedback
🍷 Network
Apply by Feb 5: forms.gle/1qk4PHjbee35...
#UXTesting #Biotech #OpenScience
@jjalston.bsky.social congrats on the Hanna Gray!
January 8, 2025 at 2:53 PM
@jjalston.bsky.social congrats on the Hanna Gray!
How can the dose be an order of magnitude lower than they first estimated, but then have zero change in their conclusion? Shouldn’t their concern about black plastic cookware be 10x lower now??
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
The Study That Called Out Black Plastic Utensils Had a Major Math Error — WIRED
Despite the correction, the study’s authors stand by their conclusions about the risks of certain kitchen utensils.
apple.news
December 21, 2024 at 2:54 PM
How can the dose be an order of magnitude lower than they first estimated, but then have zero change in their conclusion? Shouldn’t their concern about black plastic cookware be 10x lower now??
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
I still miss the tan M&Ms.
December 20, 2024 at 3:23 AM
I still miss the tan M&Ms.
Reposted by Sam Lord
“NIH reiterates that compliance with the Policy does not require the payment of an open access fee to a journal…free pathway to compliance can be achieved by depositing the Author Accepted Manuscript into PubMed Central for public availability upon the Official Date of Publication without embargo.“
Hot off the presses today is the 2024 NIH Public Access Policy. I read it so you don’t have t…Just kidding. You should still read it in full if you do NIH funded research.
grants.nih.gov/grants/guide...
Some highlights in the thread below
grants.nih.gov/grants/guide...
Some highlights in the thread below
NOT-OD-25-047: 2024 NIH Public Access Policy
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: 2024 NIH Public Access Policy NOT-OD-25-047. OD
grants.nih.gov
December 18, 2024 at 4:38 PM
“NIH reiterates that compliance with the Policy does not require the payment of an open access fee to a journal…free pathway to compliance can be achieved by depositing the Author Accepted Manuscript into PubMed Central for public availability upon the Official Date of Publication without embargo.“
Reposted by Sam Lord
Advances in microscopy mean we can now do more than just observe biology—we can control it. But how far can we really push this in mammalian cells with all their (beautiful but annoying) heterogeneity? 🧪🔬(🧵)
December 17, 2024 at 5:47 PM
Advances in microscopy mean we can now do more than just observe biology—we can control it. But how far can we really push this in mammalian cells with all their (beautiful but annoying) heterogeneity? 🧪🔬(🧵)
Speakers, try adjusting gamma on your images (e.g. 0.45) on your slides. Especially for images that are still hard to see after optimizing contrast. #CellBio2024
December 15, 2024 at 6:12 PM
Speakers, try adjusting gamma on your images (e.g. 0.45) on your slides. Especially for images that are still hard to see after optimizing contrast. #CellBio2024
Reposted by Sam Lord
Sharing my latest preprint as a re-introduction to bsky!
biorxiv.org/content/10.1...
----
Everyday, cells like this crawl through your body to protect you. While we know that their front 🔴 and back 🔵 polarity programs work together, how exactly do these two processes communicate?
biorxiv.org/content/10.1...
----
Everyday, cells like this crawl through your body to protect you. While we know that their front 🔴 and back 🔵 polarity programs work together, how exactly do these two processes communicate?
November 11, 2024 at 6:34 PM
Sharing my latest preprint as a re-introduction to bsky!
biorxiv.org/content/10.1...
----
Everyday, cells like this crawl through your body to protect you. While we know that their front 🔴 and back 🔵 polarity programs work together, how exactly do these two processes communicate?
biorxiv.org/content/10.1...
----
Everyday, cells like this crawl through your body to protect you. While we know that their front 🔴 and back 🔵 polarity programs work together, how exactly do these two processes communicate?
Reposted by Sam Lord
I love SuperPlots! If I can shamelessly share an article I wrote this year about some do's and don'ts of data viz, I also included a reproduction of the same figures of the paper in Python here: pub.ista.ac.at/~mdallave/re...
Dos and Don’ts of data visualization
pub.ista.ac.at
November 18, 2024 at 8:12 PM
I love SuperPlots! If I can shamelessly share an article I wrote this year about some do's and don'ts of data viz, I also included a reproduction of the same figures of the paper in Python here: pub.ista.ac.at/~mdallave/re...
Q: What's wrong with these plots?
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
November 18, 2024 at 7:02 PM
Q: What's wrong with these plots?
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A starter pack of starter packs...
⚠️THREAD OF ALL SCIENCE-RELATED STARTER PACKS! ⚠️
🔄Share to help people finding its topic/field
- STRUCTURAL BIOLOGY
Structural Biology 1: bsky.app/starter-pack...
Structural Biology 2: bsky.app/starter-pack...
Crystallography: bsky.app/starter-pack...
Synchrotron&CryoEM: bsky.app/starter-pack...
🔄Share to help people finding its topic/field
- STRUCTURAL BIOLOGY
Structural Biology 1: bsky.app/starter-pack...
Structural Biology 2: bsky.app/starter-pack...
Crystallography: bsky.app/starter-pack...
Synchrotron&CryoEM: bsky.app/starter-pack...
November 18, 2024 at 5:36 PM
A starter pack of starter packs...
Reposted by Sam Lord
I'm trying to find all the cool people sharing microscopy here. Help me 🤓🔬
go.bsky.app/Aaf8sKT
go.bsky.app/Aaf8sKT
November 12, 2024 at 4:08 PM
I'm trying to find all the cool people sharing microscopy here. Help me 🤓🔬
go.bsky.app/Aaf8sKT
go.bsky.app/Aaf8sKT
