Peter Rupprecht
@ptrrupprecht.bsky.social
Junior group leader in neuroscience - calcium imaging, ephys, plasticity, microscopy, neurons & astrocytes, data analysis. PhD with Rainer Friedrich, postdoc with Fritjof Helmchen. https://www.gcamp6f.com/
Pinned
Peter Rupprecht
@ptrrupprecht.bsky.social
· Mar 11
Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1...
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
Still have to read it carefully with all methods and details, but it's a candidate for my favorite paper of 2026! Very cool, and important work to understand how neurons and dendrites work!
What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...
January 6, 2026 at 5:54 PM
Still have to read it carefully with all methods and details, but it's a candidate for my favorite paper of 2026! Very cool, and important work to understand how neurons and dendrites work!
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part III - Simultaneously recording from all neurons of the human brain
gcamp6f.com/2026/01/03/a...
Part III - Simultaneously recording from all neurons of the human brain
gcamp6f.com/2026/01/03/a...
Annual report of my intuition about the brain (2025, part III)
How does the brain work, and how can we understand it? To approach this big question from a broad perspective, I want to report on some ideas about the brain that marked me most over the …
gcamp6f.com
January 6, 2026 at 5:49 PM
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part III - Simultaneously recording from all neurons of the human brain
gcamp6f.com/2026/01/03/a...
Part III - Simultaneously recording from all neurons of the human brain
gcamp6f.com/2026/01/03/a...
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part II - Recording the inputs and the output of a single neuron in real time in vivo
gcamp6f.com/2025/12/30/a...
Part II - Recording the inputs and the output of a single neuron in real time in vivo
gcamp6f.com/2025/12/30/a...
Annual report of my intuition about the brain (2025, part III)
How does the brain work, and how can we understand it? To approach this big question from a broad perspective, I want to report on some ideas about the brain that marked me most over the …
gcamp6f.com
January 6, 2026 at 5:47 PM
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part II - Recording the inputs and the output of a single neuron in real time in vivo
gcamp6f.com/2025/12/30/a...
Part II - Recording the inputs and the output of a single neuron in real time in vivo
gcamp6f.com/2025/12/30/a...
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part I - connectomics
gcamp6f.com/2025/12/27/a...
Part I - connectomics
gcamp6f.com/2025/12/27/a...
Annual report of my intuition about the brain (2025, part I)
How does the brain work, and how can we understand it? To approach this big question from a broad perspective, I want to report on some ideas about the brain that marked me most over the …
gcamp6f.com
January 6, 2026 at 5:45 PM
What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post.
Part I - connectomics
gcamp6f.com/2025/12/27/a...
Part I - connectomics
gcamp6f.com/2025/12/27/a...
Reposted by Peter Rupprecht
New preprint from the lab! 🚀
We find that hippocampal OLM interneurons provide a circuit-level inhibitory feedback signal that dynamically controls when and where behavioral timescale synaptic plasticity can occur.
Feedback welcome!
We find that hippocampal OLM interneurons provide a circuit-level inhibitory feedback signal that dynamically controls when and where behavioral timescale synaptic plasticity can occur.
Feedback welcome!
Dendrite-targeting OLM interneurons regulate the formation of learning-related CA1 place cell representations https://www.biorxiv.org/content/10.64898/2025.12.21.695825v1
January 5, 2026 at 3:11 PM
New preprint from the lab! 🚀
We find that hippocampal OLM interneurons provide a circuit-level inhibitory feedback signal that dynamically controls when and where behavioral timescale synaptic plasticity can occur.
Feedback welcome!
We find that hippocampal OLM interneurons provide a circuit-level inhibitory feedback signal that dynamically controls when and where behavioral timescale synaptic plasticity can occur.
Feedback welcome!
Reposted by Peter Rupprecht
What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...
January 4, 2026 at 6:17 AM
What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...
All the work by @colah.bsky.social (blog posts, distill etc.) is amazing - clear and transparent, with little or no jargon, trying to address the hard problems head-on.
December 16, 2025 at 11:50 AM
All the work by @colah.bsky.social (blog posts, distill etc.) is amazing - clear and transparent, with little or no jargon, trying to address the hard problems head-on.
Reposted by Peter Rupprecht
Thankful for this blog piece by @ptrrupprecht.bsky.social on the Dirigo project. Glad you liked the posts!
gcamp6f.com/2025/11/14/d...
gcamp6f.com/2025/11/14/d...
Dirigo: a future Python alternative to Scanimage?
Over the last 20 years, many microscopes that are capable of resonant scanning two-photon microscopy have converged on using ScanImage, a powerful software package with many strengths but also two …
gcamp6f.com
November 21, 2025 at 4:24 PM
Thankful for this blog piece by @ptrrupprecht.bsky.social on the Dirigo project. Glad you liked the posts!
gcamp6f.com/2025/11/14/d...
gcamp6f.com/2025/11/14/d...
But I believe that post-publication reviews, or the suggestions that you've made, or simply transparent open reviews, which is used by many journals already, are a good way to read papers better and more efficiently. And this is a culture change that has already taken place during the last decade...
November 21, 2025 at 10:39 AM
But I believe that post-publication reviews, or the suggestions that you've made, or simply transparent open reviews, which is used by many journals already, are a good way to read papers better and more efficiently. And this is a culture change that has already taken place during the last decade...
I don't think there is a way around properly reading the literature. How could we design science experiments, if the knowledge that we need for their design is somewhere in a database or LLM, but not in our heads?
November 21, 2025 at 10:38 AM
I don't think there is a way around properly reading the literature. How could we design science experiments, if the knowledge that we need for their design is somewhere in a database or LLM, but not in our heads?
Good point! But already now, everybody can contribute to post-publication peer review right now, no need for a centralized structure. I regularly write such reviews on my blog: gcamp6f.com/category/rev...
The idea is not new, see the "peeriodicals" by Romain Brette:
peeriodicals.com/peeriodicals...
The idea is not new, see the "peeriodicals" by Romain Brette:
peeriodicals.com/peeriodicals...
Peeriodicals
Theory of spike initiation, sensory systems, autonomous behavior, epistemology
peeriodicals.com
November 20, 2025 at 8:59 PM
Good point! But already now, everybody can contribute to post-publication peer review right now, no need for a centralized structure. I regularly write such reviews on my blog: gcamp6f.com/category/rev...
The idea is not new, see the "peeriodicals" by Romain Brette:
peeriodicals.com/peeriodicals...
The idea is not new, see the "peeriodicals" by Romain Brette:
peeriodicals.com/peeriodicals...
Reposted by Peter Rupprecht
Independent NeuroScience Services INSS was registered as a company on 14th November 2016, which means it's our 9th birthday today! As a birthday present we've treated ourselves to an SfN exhibitor booth for the first time ever. Come and say hello at Booth #3327
November 15, 2025 at 12:03 AM
Independent NeuroScience Services INSS was registered as a company on 14th November 2016, which means it's our 9th birthday today! As a birthday present we've treated ourselves to an SfN exhibitor booth for the first time ever. Come and say hello at Booth #3327
If you don't learn how to write, then you don't learn how to think.
November 15, 2025 at 3:52 PM
If you don't learn how to write, then you don't learn how to think.
New blog post, where I highlight a few interesting papers on astrocyte ultrastructure and function, with papers from the labs of Moritz Helmstädter @mhlab.bsky.social, Kristen Harris, Andrea Volterra, Karin Pernet-Gallay, and Hailan Hu: gcamp6f.com/2025/11/14/i...
Interesting papers on astrocyte ultrastructure and function
Astrocytes are brain cells that are often completely overlooked and dismissed or, in the opposite extreme, presented as mysterious devices that somehow solve all problems of computational neuroscie…
gcamp6f.com
November 14, 2025 at 12:27 PM
New blog post, where I highlight a few interesting papers on astrocyte ultrastructure and function, with papers from the labs of Moritz Helmstädter @mhlab.bsky.social, Kristen Harris, Andrea Volterra, Karin Pernet-Gallay, and Hailan Hu: gcamp6f.com/2025/11/14/i...
Reposted by Peter Rupprecht
How did I not know about this super-useful site for customized 3D-printable thread adapters? HT to @leon-kremers.bsky.social : brainright.com/adapt-o-matic/
Jay’s Adapt~O~Matic – BrainRight
brainright.com
November 5, 2025 at 11:02 AM
How did I not know about this super-useful site for customized 3D-printable thread adapters? HT to @leon-kremers.bsky.social : brainright.com/adapt-o-matic/
Reposted by Peter Rupprecht
The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...
October 22, 2025 at 6:47 PM
The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...
Looks like a 1:1 copy of the Cambridge Technology 8 kHz scanner. Did you notice any differences? Or have they simply licensed the technology from the existing scanner?
October 22, 2025 at 7:58 PM
Looks like a 1:1 copy of the Cambridge Technology 8 kHz scanner. Did you notice any differences? Or have they simply licensed the technology from the existing scanner?
It's a great paper - congrats!
October 3, 2025 at 8:25 PM
It's a great paper - congrats!
This objective was designed for 2P imaging. For 1P imaging, I believe that there are still some technical problems to be solved. Perhaps @voigtvision.bsky.social or @nvladimus.bsky.social can tell you more!
September 29, 2025 at 9:15 AM
This objective was designed for 2P imaging. For 1P imaging, I believe that there are still some technical problems to be solved. Perhaps @voigtvision.bsky.social or @nvladimus.bsky.social can tell you more!
I'm using it mostly for 2P imaging, but it should also be relevant for expansion microscopy: Olympus XLPLN10XSVMP with NA 0.6, WD 8 mm: evidentscientific.com/en/objective...
Objective Finder | Evident Scientific | Olympus
Select the right lens for your application from our broad line of Olympus microscope objectives.
evidentscientific.com
September 28, 2025 at 6:22 PM
I'm using it mostly for 2P imaging, but it should also be relevant for expansion microscopy: Olympus XLPLN10XSVMP with NA 0.6, WD 8 mm: evidentscientific.com/en/objective...
Reflective Schmidt objectives with high NA and long WD: www.nature.com/articles/s41...
@voigtvision.bsky.social
@voigtvision.bsky.social
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope - Nature Biotechnology
Imaging of large, cleared samples in diverse media is achieved using a mirror objective.
www.nature.com
September 28, 2025 at 6:19 PM
Reflective Schmidt objectives with high NA and long WD: www.nature.com/articles/s41...
@voigtvision.bsky.social
@voigtvision.bsky.social
Reposted by Peter Rupprecht
Understanding behavior requires datasets that capture humans while carrying out complex tasks. The kitchen is an excellent environment for assessing human motor and cognitive function, as many complex actions are naturally exhibited in kitchens from chopping to 🧽!
arxiv.org/abs/2506.01608
arxiv.org/abs/2506.01608
EPFL-Smart-Kitchen-30: Densely annotated cooking dataset with 3D kinematics to challenge video and language models
Understanding behavior requires datasets that capture humans while carrying out complex tasks. The kitchen is an excellent environment for assessing human motor and cognitive function, as many complex...
arxiv.org
September 23, 2025 at 7:18 AM
Understanding behavior requires datasets that capture humans while carrying out complex tasks. The kitchen is an excellent environment for assessing human motor and cognitive function, as many complex actions are naturally exhibited in kitchens from chopping to 🧽!
arxiv.org/abs/2506.01608
arxiv.org/abs/2506.01608
Reposted by Peter Rupprecht
« Diverse calcium dynamics underlie place field formation in hippocampal CA1 pyramidal cells. »
A fundamental study now published @elife.bsky.social
elifesciences.org/reviewed-pre...
A fundamental study now published @elife.bsky.social
elifesciences.org/reviewed-pre...
September 17, 2025 at 12:05 PM
« Diverse calcium dynamics underlie place field formation in hippocampal CA1 pyramidal cells. »
A fundamental study now published @elife.bsky.social
elifesciences.org/reviewed-pre...
A fundamental study now published @elife.bsky.social
elifesciences.org/reviewed-pre...
New blog post about a fun project where I tried to beautify my office by putting a poster of the hippocampal column onto my walls: gcamp6f.com/2025/08/13/d...
EM pictures from this paper: www.biorxiv.org/content/10.1... from the lab of @helenelab.bsky.social
EM pictures from this paper: www.biorxiv.org/content/10.1... from the lab of @helenelab.bsky.social
Displaying the hippocampus as EM image on the office wall
One of the most amazing methods in modern neuroscience is dense volumetric electron microscopy of the brain. I have mentioned some openly accessible datasets before (see my previous blog post), but…
gcamp6f.com
August 14, 2025 at 8:27 AM
New blog post about a fun project where I tried to beautify my office by putting a poster of the hippocampal column onto my walls: gcamp6f.com/2025/08/13/d...
EM pictures from this paper: www.biorxiv.org/content/10.1... from the lab of @helenelab.bsky.social
EM pictures from this paper: www.biorxiv.org/content/10.1... from the lab of @helenelab.bsky.social
New blog post, where I review some recent interesting papers on the role of cell organelles for neuronal calcium signaling: gcamp6f.com/2025/08/11/i...
Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!
Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!
Interesting papers on the role of cell organelles in neuronal dendrites for calcium signaling
Researchers utilize calcium imaging to track neuronal activity in the brain, highlighting the role of calcium as a signaling molecule. Recent studies investigate intracellular calcium dynamics, par…
gcamp6f.com
August 11, 2025 at 12:19 PM
New blog post, where I review some recent interesting papers on the role of cell organelles for neuronal calcium signaling: gcamp6f.com/2025/08/11/i...
Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!
Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!