@voigtvision.bsky.social
🔬 & 👁️ & 🐟-News & Views by Fabian Voigt - Branco Weiss Fellow in
the Engert lab at MCB @harvard.edu. Previously at UZH Zurich with Fritjof Helmchen. #mesoSPIM developer
the Engert lab at MCB @harvard.edu. Previously at UZH Zurich with Fritjof Helmchen. #mesoSPIM developer
That's super cool, congrats! 🥳
December 20, 2025 at 12:02 PM
That's super cool, congrats! 🥳
(And that scan lens is essentially a really nice NA 0.125 microscope objective with 32 mm FOV and an accessible pupil location)
December 20, 2025 at 2:41 AM
(And that scan lens is essentially a really nice NA 0.125 microscope objective with 32 mm FOV and an accessible pupil location)
The sup material of that same paper includes their scan lens design - so it seems like they designed it as a combo.
December 20, 2025 at 2:37 AM
The sup material of that same paper includes their scan lens design - so it seems like they designed it as a combo.
The Handbook of optical systems, Vol 4: Survey of optical instruments, Ch 7 on Microscope Optics (p 646) gives a few more reasons (very good chapter btw).
December 19, 2025 at 3:20 PM
The Handbook of optical systems, Vol 4: Survey of optical instruments, Ch 7 on Microscope Optics (p 646) gives a few more reasons (very good chapter btw).
The big exception are stereo-microscopes and macro-zoom systems: Building a zoom system that's fully telecentric across all magnifications is not that trivial, so usually, the way macro systems are designed is that they get more telecentric with higher NA (where the effects are more apparent)
December 19, 2025 at 3:13 PM
The big exception are stereo-microscopes and macro-zoom systems: Building a zoom system that's fully telecentric across all magnifications is not that trivial, so usually, the way macro systems are designed is that they get more telecentric with higher NA (where the effects are more apparent)
Similarly, in a classical wide-field microscope, being non-telecentric would mean that objects get bigger or smaller as you focus up and down - so they get blurry and change size. That's quite annoying as well, especially for visual use
December 19, 2025 at 3:11 PM
Similarly, in a classical wide-field microscope, being non-telecentric would mean that objects get bigger or smaller as you focus up and down - so they get blurry and change size. That's quite annoying as well, especially for visual use
I'm quite sure that all of these are telecentric or as close as possible. If you have a correction collar and expect varying media, being non-telecentric would mean that you have medium-dependent magnification (because the chief ray get's refracted differently at the cover slip) - that's annoying
December 19, 2025 at 3:09 PM
I'm quite sure that all of these are telecentric or as close as possible. If you have a correction collar and expect varying media, being non-telecentric would mean that you have medium-dependent magnification (because the chief ray get's refracted differently at the cover slip) - that's annoying
You can still design tube lens + objective as a matched pair in the latter case and it will make the whole thing a little easier to balance. As usual, it's a high dimensional design space and there's many solutions that can make biologists happy
December 19, 2025 at 12:51 AM
You can still design tube lens + objective as a matched pair in the latter case and it will make the whole thing a little easier to balance. As usual, it's a high dimensional design space and there's many solutions that can make biologists happy
This is what Dan Flickinger did for the system that became the Janelia/Thorlabs multiphoton mesoscope: elifesciences.org/articles/14472
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging
An optical microscopy approach with an ultra-large field of view but retained subcellular resolution allows simultaneous imaging of neural activity in widely dispersed brain regions.
elifesciences.org
December 19, 2025 at 12:27 AM
This is what Dan Flickinger did for the system that became the Janelia/Thorlabs multiphoton mesoscope: elifesciences.org/articles/14472
In my view, the trade-off is not really worth it. My suggestion would be to not just design the objective, but a whole objective/tubelens/scan lens combo that works together - this way, you can balance aberrations (in particular chromatic etc.) between them.
December 19, 2025 at 12:26 AM
In my view, the trade-off is not really worth it. My suggestion would be to not just design the objective, but a whole objective/tubelens/scan lens combo that works together - this way, you can balance aberrations (in particular chromatic etc.) between them.
Yes, you can force a different pupil location onto an objective - essentially that's what's happening in a misaligned laser scanning scope or an OPM with a wrongly matched O1/O2 combo. The trade-off is that you might see other artifacts - for example vignetting, higher aberrations...
December 19, 2025 at 12:22 AM
Yes, you can force a different pupil location onto an objective - essentially that's what's happening in a misaligned laser scanning scope or an OPM with a wrongly matched O1/O2 combo. The trade-off is that you might see other artifacts - for example vignetting, higher aberrations...
This figure is from Sup Figure 1 of the 2019 @mesospim.bsky.social paper: www.nature.com/articles/s41...
The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue - Nature Methods
The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.
www.nature.com
December 18, 2025 at 9:09 PM
This figure is from Sup Figure 1 of the 2019 @mesospim.bsky.social paper: www.nature.com/articles/s41...
Telecentricity will make integration into custom scopes much easier. Not being telecentric means that your magnification/FOV depends on the working distance: Now imagine building an OPM with it: If it's not telecentric, the lightsheet will change its lateral width along its propagation direction
December 18, 2025 at 9:05 PM
Telecentricity will make integration into custom scopes much easier. Not being telecentric means that your magnification/FOV depends on the working distance: Now imagine building an OPM with it: If it's not telecentric, the lightsheet will change its lateral width along its propagation direction
Reposted
We're still exploring how to improve LSFM performance with "big" detection air objectives, where field curvature often limits achievable FOV. Here, Steven introduces curvedASLM, a method to dynamically correct field curvature (along one axis) in axially scanned setups www.biorxiv.org/content/10.6...
December 10, 2025 at 8:12 PM
We're still exploring how to improve LSFM performance with "big" detection air objectives, where field curvature often limits achievable FOV. Here, Steven introduces curvedASLM, a method to dynamically correct field curvature (along one axis) in axially scanned setups www.biorxiv.org/content/10.6...
Reposted
The EMBO Practical Course "Hack your microscope" in Oeiras, PT, 20–25 Apr 2026 aims to innovate, democratize advanced #imaging technologies and shape the future of open-source #microscopy – Apply now!
Deadline: 12 Dec
https://meetings.embo.org/event/26-microscope
#EMBOmicroscope #EMBOevents 🧪
Deadline: 12 Dec
https://meetings.embo.org/event/26-microscope
#EMBOmicroscope #EMBOevents 🧪
December 3, 2025 at 4:15 PM
The EMBO Practical Course "Hack your microscope" in Oeiras, PT, 20–25 Apr 2026 aims to innovate, democratize advanced #imaging technologies and shape the future of open-source #microscopy – Apply now!
Deadline: 12 Dec
https://meetings.embo.org/event/26-microscope
#EMBOmicroscope #EMBOevents 🧪
Deadline: 12 Dec
https://meetings.embo.org/event/26-microscope
#EMBOmicroscope #EMBOevents 🧪
Supercool work! What's the difference between the lite version ( github.com/uhlmanngroup... ) and the original version ( github.com/uhlmanngroup... ) from a user perspective? Also, how complicated/painful would it be to extend this to 3D?
GitHub - uhlmanngroup/ShapeEmbedLite: ShapeEmbedLite: a lightweight self-supervised representation learning model for 2D shape analysis
ShapeEmbedLite: a lightweight self-supervised representation learning model for 2D shape analysis - uhlmanngroup/ShapeEmbedLite
github.com
December 2, 2025 at 2:05 PM
Supercool work! What's the difference between the lite version ( github.com/uhlmanngroup... ) and the original version ( github.com/uhlmanngroup... ) from a user perspective? Also, how complicated/painful would it be to extend this to 3D?
Say hi to Alain! Hope you all have a fun SfN!
November 17, 2025 at 5:19 PM
Say hi to Alain! Hope you all have a fun SfN!
As far as I understand, it's a complete redesign with the same specs and mechanical housing as the CRS8k to allow backward-compatibility.
October 23, 2025 at 4:59 PM
As far as I understand, it's a complete redesign with the same specs and mechanical housing as the CRS8k to allow backward-compatibility.
The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...
October 22, 2025 at 6:47 PM
The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...
Philipp Keller, Benquan Wang, and Dan Flickinger at Janelia have designed a mirror objective for expansion samples that has NA 1 over a 12 mm FOV - a superimpressive setup! The only thing that's out about it is a patent application: www.freepatentsonline.com/y2023/000398...
September 29, 2025 at 1:27 PM
Philipp Keller, Benquan Wang, and Dan Flickinger at Janelia have designed a mirror objective for expansion samples that has NA 1 over a 12 mm FOV - a superimpressive setup! The only thing that's out about it is a patent application: www.freepatentsonline.com/y2023/000398...
Thomas Naert recently published a paper on a toolbox for DNA editing with some Schmidt objective data: www.nature.com/articles/s41...
Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates - Nature Biotechnology
Genomic integration of DNA templates is made more precise through microhomology-focused design.
www.nature.com
September 29, 2025 at 1:22 PM
Thomas Naert recently published a paper on a toolbox for DNA editing with some Schmidt objective data: www.nature.com/articles/s41...
One of the people I'm indebted the most for creating stunningly beautiful #mesoSPIM samples was Martina Schaettin - who tragically passed away in March this year. To honor her memory, Elkhan Yusifov created an award - submit your best #microscopy images & videos! www.linkedin.com/company/mart...
August 22, 2025 at 5:32 PM
One of the people I'm indebted the most for creating stunningly beautiful #mesoSPIM samples was Martina Schaettin - who tragically passed away in March this year. To honor her memory, Elkhan Yusifov created an award - submit your best #microscopy images & videos! www.linkedin.com/company/mart...
Thanks to @nvladimus.bsky.social the @mesospim.bsky.social #lightsheet #Microscope community is now on forum.image.sc/tag/mesospim !
📣We have opened our official User Forum: forum.image.sc/tag/mesospim
In this forum you can connect with 🔬developers and the image analysis experts. Ask your burning questions about the #mesoSPIM hardware, software, and image processing, or help the beginners to get started!
Tip: use project tags!
In this forum you can connect with 🔬developers and the image analysis experts. Ask your burning questions about the #mesoSPIM hardware, software, and image processing, or help the beginners to get started!
Tip: use project tags!
Topics tagged mesospim
Topics tagged mesospim
forum.image.sc
August 22, 2025 at 3:58 PM
Thanks to @nvladimus.bsky.social the @mesospim.bsky.social #lightsheet #Microscope community is now on forum.image.sc/tag/mesospim !
Definitely the most unexpected @mesospim.bsky.social #lightsheet #microscopy application of the past years: Hunting dark matter with a lightsheet microscope! #astronomy 🔭🔬https://arxiv.org/abs/2503.20732
💥How can light-sheet help reveal dark matter?💥
Join Prof. Laura Baudis @lbaudis.bsky.social at #mesoSPIM Symposium, Oct 13–15 in Zurich or online, to see how light-sheet fluorescence microscopy 🔬 is used to image nuclear ☢️ recoil tracks to detect elusive particles!
Register now: t.uzh.ch/1Ss
Join Prof. Laura Baudis @lbaudis.bsky.social at #mesoSPIM Symposium, Oct 13–15 in Zurich or online, to see how light-sheet fluorescence microscopy 🔬 is used to image nuclear ☢️ recoil tracks to detect elusive particles!
Register now: t.uzh.ch/1Ss
August 21, 2025 at 1:39 PM
Definitely the most unexpected @mesospim.bsky.social #lightsheet #microscopy application of the past years: Hunting dark matter with a lightsheet microscope! #astronomy 🔭🔬https://arxiv.org/abs/2503.20732
#Deeplearning-based #CRISPR DNA integration and editing by Thomas Naert & Sören Lienkamp & Co with lots of @mesospim.bsky.social #lightsheet #Microscopy data of tadpoles (and some taken with the #Schmidt #multiimmersion objective prototype!) 🔬🐸 www.nature.com/articles/s41...
August 13, 2025 at 2:09 PM
#Deeplearning-based #CRISPR DNA integration and editing by Thomas Naert & Sören Lienkamp & Co with lots of @mesospim.bsky.social #lightsheet #Microscopy data of tadpoles (and some taken with the #Schmidt #multiimmersion objective prototype!) 🔬🐸 www.nature.com/articles/s41...