Johannes Bohacek
@bohaceklab.bsky.social
Dad. Associate Professor @ ETH Zurich. We study stress, behavior, hippocampus, noradrenaline and the mighty locus coeruleus. We work with mice and focus a lot on 3Rs.
Here to learn, share, laugh and rant.
Here to learn, share, laugh and rant.
Pinned
Distinct molecular mechanisms of stress habituation in the mouse hippocampus https://www.biorxiv.org/content/10.1101/2025.03.04.641433v1
I’m trilled to release the largest amount of stress profiling data from our (I think any) lab so far. We asked how the molecular response to acute stress adapts as the stressor becomes chronic. Multiomic profiling in the mouse hippocampus, led by Rebecca Waag and co-last author Pierre-Luc Germain 💪
🔵 I'm interrupting my social media hiatus to flag this important preprint from the Bruchas lab (is he not on Bluesky!?) together with @davidweinshenker.bsky.social. Very difficult experiments to show that dopamine release from LC terminals is independent of VTA 🔥
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
September 17, 2025 at 5:44 AM
🔵 I'm interrupting my social media hiatus to flag this important preprint from the Bruchas lab (is he not on Bluesky!?) together with @davidweinshenker.bsky.social. Very difficult experiments to show that dopamine release from LC terminals is independent of VTA 🔥
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
This preprint has been under review >110 days... I think they can't find reviewers.
WOULD YOU BE WILLING TO SERVE AS A REVIEWER?
If you're keen, I'd propose your name to the editor...
Please re-post
WOULD YOU BE WILLING TO SERVE AS A REVIEWER?
If you're keen, I'd propose your name to the editor...
Please re-post
How much analgesia is needed to manage pain levels in mice after brain surgeries?
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
GrimACE: Automated, multimodal cage-side assessment of pain and well-being in mice
Pain and welfare monitoring is essential for ethical animal testing, but current cage-side assessments are qualitative and subjective. Here we present the GrimACE, the first fully standardised and aut...
www.biorxiv.org
June 27, 2025 at 11:43 AM
This preprint has been under review >110 days... I think they can't find reviewers.
WOULD YOU BE WILLING TO SERVE AS A REVIEWER?
If you're keen, I'd propose your name to the editor...
Please re-post
WOULD YOU BE WILLING TO SERVE AS A REVIEWER?
If you're keen, I'd propose your name to the editor...
Please re-post
I'd have so much to complain about these days. But given the state of affairs in the world, it feels inappropriate to whine about daily hassles. So let me just say this on behalf of everyone who is struggling but otherwise safe: fucking hell.
June 18, 2025 at 4:35 PM
I'd have so much to complain about these days. But given the state of affairs in the world, it feels inappropriate to whine about daily hassles. So let me just say this on behalf of everyone who is struggling but otherwise safe: fucking hell.
🔵 Interesting new work from @davidweinshenker.bsky.social on why locus coeruleus neurons might be vulnerable to degeneration in humans (Alzheimer's) but not in mice. And a new approach on how this could be modelled in mice through human tyrosinase overexpression: www.biorxiv.org/content/10.1...
Tyrosinase-induced neuromelanin accumulation triggers rapid dysregulation and degeneration of the mouse locus coeruleus
The locus coeruleus (LC), the major source of norepinephrine (NE) in the brain, is among the earliest site of pathology in both Alzheimers disease (AD) and Parkinsons disease (PD), and it undergoes ca...
www.biorxiv.org
April 9, 2025 at 7:25 PM
🔵 Interesting new work from @davidweinshenker.bsky.social on why locus coeruleus neurons might be vulnerable to degeneration in humans (Alzheimer's) but not in mice. And a new approach on how this could be modelled in mice through human tyrosinase overexpression: www.biorxiv.org/content/10.1...
Impression from the evening panel discussion at #ESC2025. Amazing talks today, wonderfully open and fun atmosphere. After all these years, still my favorite meeting in the conference cycle ❤️
March 17, 2025 at 6:44 PM
Impression from the evening panel discussion at #ESC2025. Amazing talks today, wonderfully open and fun atmosphere. After all these years, still my favorite meeting in the conference cycle ❤️
Reposted by Johannes Bohacek
Dopamine and acetylcholine interactions - so hot right now! Tour de force work from Steph Cragg's group out in NN (lead Yan-Feng Zhang). They find Cholinergic Interneurons (ChIs) largely suppress DA release, distinct from what we just reported in songbirds (1/x)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 17, 2025 at 1:24 PM
Dopamine and acetylcholine interactions - so hot right now! Tour de force work from Steph Cragg's group out in NN (lead Yan-Feng Zhang). They find Cholinergic Interneurons (ChIs) largely suppress DA release, distinct from what we just reported in songbirds (1/x)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Stellar women-only (💪) panel on stress and development, loaded with cutting-edge multiomic data kicking off the European Stress Conference #ESC2025 in Innsbruck, Austria
March 16, 2025 at 9:53 AM
Stellar women-only (💪) panel on stress and development, loaded with cutting-edge multiomic data kicking off the European Stress Conference #ESC2025 in Innsbruck, Austria
Reposted by Johannes Bohacek
My lab is hiring 2 postdocs on an NIH-funded grant characterizing molecular & cellular changes in the human brain across menopausal transition. Other neuroepigenomics projects are also available. We plan to hire a wet lab scientist and a bioinformatician. Please RT!
kundakoviclab.com
More info 👇
kundakoviclab.com
More info 👇
March 11, 2025 at 6:32 AM
My lab is hiring 2 postdocs on an NIH-funded grant characterizing molecular & cellular changes in the human brain across menopausal transition. Other neuroepigenomics projects are also available. We plan to hire a wet lab scientist and a bioinformatician. Please RT!
kundakoviclab.com
More info 👇
kundakoviclab.com
More info 👇
How much analgesia is needed to manage pain levels in mice after brain surgeries?
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
GrimACE: Automated, multimodal cage-side assessment of pain and well-being in mice
Pain and welfare monitoring is essential for ethical animal testing, but current cage-side assessments are qualitative and subjective. Here we present the GrimACE, the first fully standardised and aut...
www.biorxiv.org
March 11, 2025 at 7:00 AM
How much analgesia is needed to manage pain levels in mice after brain surgeries?
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...
I’m trilled to release the largest amount of stress profiling data from our (I think any) lab so far. We asked how the molecular response to acute stress adapts as the stressor becomes chronic. Multiomic profiling in the mouse hippocampus, led by Rebecca Waag and co-last author Pierre-Luc Germain 💪
Distinct molecular mechanisms of stress habituation in the mouse hippocampus https://www.biorxiv.org/content/10.1101/2025.03.04.641433v1
March 6, 2025 at 9:12 PM
I’m trilled to release the largest amount of stress profiling data from our (I think any) lab so far. We asked how the molecular response to acute stress adapts as the stressor becomes chronic. Multiomic profiling in the mouse hippocampus, led by Rebecca Waag and co-last author Pierre-Luc Germain 💪
Uuuhh... DANNCE is back! Now social-DANNCE. Looks very cool, taking multi-animal tracking and analysis of social behavior to a whole new level. Just the green-room multiple-camera setup looks quite complex, I wonder how easy this can be replicated in other labs. Will certainly spark excitement!
Excited to present the latest from the lab out today in Cell www.cell.com/cell/fulltex.... See Thread! 1/8
March 4, 2025 at 6:15 PM
Uuuhh... DANNCE is back! Now social-DANNCE. Looks very cool, taking multi-animal tracking and analysis of social behavior to a whole new level. Just the green-room multiple-camera setup looks quite complex, I wonder how easy this can be replicated in other labs. Will certainly spark excitement!
Reposted by Johannes Bohacek
SAVE THE DATE: #resilience2025 - the 11th International Symposium on Resilience Research - Mainz, Germany - September 24-26, 2025
Satellite methods workshop: September 23 & many opportunities for early-career researchers
Meet the stress resilience research community, get latest insights, spur you
Satellite methods workshop: September 23 & many opportunities for early-career researchers
Meet the stress resilience research community, get latest insights, spur you
December 4, 2024 at 12:52 PM
SAVE THE DATE: #resilience2025 - the 11th International Symposium on Resilience Research - Mainz, Germany - September 24-26, 2025
Satellite methods workshop: September 23 & many opportunities for early-career researchers
Meet the stress resilience research community, get latest insights, spur you
Satellite methods workshop: September 23 & many opportunities for early-career researchers
Meet the stress resilience research community, get latest insights, spur you
Is this still the place to post scientific updates about papers, preprints and cool new data? Asking for a friend.
March 4, 2025 at 3:32 PM
Is this still the place to post scientific updates about papers, preprints and cool new data? Asking for a friend.
Reposted by Johannes Bohacek
Amazing work from our colleagues at ETH/UZH answering many unknowns about arousal during sleep. And congrats to their participants for being able to sleep with their eyes taped open!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Pupil size reveals arousal level fluctuations in human sleep - Nature Communications
Arousal levels regulate sleep, but accurate noninvasive measures are lacking. Here, a novel method is introduced to track pupil-indexed arousal levels during human sleep, revealing pupil size changes ...
www.nature.com
March 2, 2025 at 11:52 AM
Amazing work from our colleagues at ETH/UZH answering many unknowns about arousal during sleep. And congrats to their participants for being able to sleep with their eyes taped open!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
First day of the new semester. 5th time I'm teaching this class. Got tenured along the way. And still, I'm nervous before going in... 😅🤷♂️
February 17, 2025 at 8:52 AM
First day of the new semester. 5th time I'm teaching this class. Got tenured along the way. And still, I'm nervous before going in... 😅🤷♂️
Reposted by Johannes Bohacek
delighted to share our work revealing a new type of excitatory hippocampal neuron we call the "ovoid cell", which has really exquisite properties relative to adjacent pyramidal cells! superb work of first-author @adriennekinman.bsky.social and many others in the lab
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Atypical hippocampal excitatory neurons express and govern object memory - Nature Communications
Pyramidal cells are classically thought to comprise the excitatory output of the subiculum. Here, the authors show the existence of “ovoid cells”, excitatory subiculum neurons with specialized gene ex...
www.nature.com
February 13, 2025 at 6:38 PM
delighted to share our work revealing a new type of excitatory hippocampal neuron we call the "ovoid cell", which has really exquisite properties relative to adjacent pyramidal cells! superb work of first-author @adriennekinman.bsky.social and many others in the lab
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Check out this cool study by the @markcembrowski.bsky.social lab!
They describe a new hippocampus cell population that responds to novel stimuli.
But hold on to your seat: they don't if the object is presented again. Even 100 days later! When the behavioral memory has long faded!! 🤯🤯😱🤩
They describe a new hippocampus cell population that responds to novel stimuli.
But hold on to your seat: they don't if the object is presented again. Even 100 days later! When the behavioral memory has long faded!! 🤯🤯😱🤩
Atypical hippocampal excitatory neurons express and govern object memory
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Atypical hippocampal excitatory neurons express and govern object memory - Nature Communications
Pyramidal cells are classically thought to comprise the excitatory output of the subiculum. Here, the authors show the existence of “ovoid cells”, excitatory subiculum neurons with specialized gene ex...
www.nature.com
February 12, 2025 at 7:58 PM
Check out this cool study by the @markcembrowski.bsky.social lab!
They describe a new hippocampus cell population that responds to novel stimuli.
But hold on to your seat: they don't if the object is presented again. Even 100 days later! When the behavioral memory has long faded!! 🤯🤯😱🤩
They describe a new hippocampus cell population that responds to novel stimuli.
But hold on to your seat: they don't if the object is presented again. Even 100 days later! When the behavioral memory has long faded!! 🤯🤯😱🤩
Huh... tumors can have excitable neuron-like cells. And the other tumor cells support them metabolically, akin to the neuron-astrocyte relationship. 🤯
"Intrinsic electrical activity drives small-cell lung cancer progression"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Intrinsic electrical activity drives small-cell lung cancer progression - Nature
Electrical excitability in neuroendocrine SCLC cells promotes tumour progression through action potential firing, increasing ATP demand and oxidative phosphorylation dependency, whereas non-neuroendoc...
www.nature.com
February 12, 2025 at 7:37 PM
Huh... tumors can have excitable neuron-like cells. And the other tumor cells support them metabolically, akin to the neuron-astrocyte relationship. 🤯
This quote is the reward of agreeing to review a PhD thesis on very short notice. I had exactly the same experience, I just couldn't have phrased it so well. #Psychedelics
February 6, 2025 at 9:25 PM
This quote is the reward of agreeing to review a PhD thesis on very short notice. I had exactly the same experience, I just couldn't have phrased it so well. #Psychedelics
It's that time of year, I'm updating my lectures and I have weird questions.
BACKGROUND:
I've used this graph for many years to joke that sex is more stressful than anything else (and to make the more serious point that CORT levels are a poor readout for stress). ars.els-cdn.com/content/imag...
BACKGROUND:
I've used this graph for many years to joke that sex is more stressful than anything else (and to make the more serious point that CORT levels are a poor readout for stress). ars.els-cdn.com/content/imag...
January 21, 2025 at 2:48 PM
It's that time of year, I'm updating my lectures and I have weird questions.
BACKGROUND:
I've used this graph for many years to joke that sex is more stressful than anything else (and to make the more serious point that CORT levels are a poor readout for stress). ars.els-cdn.com/content/imag...
BACKGROUND:
I've used this graph for many years to joke that sex is more stressful than anything else (and to make the more serious point that CORT levels are a poor readout for stress). ars.els-cdn.com/content/imag...
Reposted by Johannes Bohacek
For LC researchers on BlueSpot Slack but haven't checked it, @mikekelberman.bsky.social and Ellen Rodberg will present their work on LC ephys tomorrow at 11 am EST. Link on General channel. If you are not on BlueSpot and want to attend, email me dweinsh@emory.edu.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Diversity of ancestral brainstem noradrenergic neurons across species and multiple biological factors
The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution has woven the LC into wide-ranging neural circuits that influence functions as broad as autonomi...
www.biorxiv.org
January 13, 2025 at 8:16 PM
For LC researchers on BlueSpot Slack but haven't checked it, @mikekelberman.bsky.social and Ellen Rodberg will present their work on LC ephys tomorrow at 11 am EST. Link on General channel. If you are not on BlueSpot and want to attend, email me dweinsh@emory.edu.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
🔵 #LocusCoeruleus alert!
Holy cow... I'm supposed to do something else, but this paper looks amazing! I mean the concept, the implications... wow.
www.cell.com/cell/fulltex...
Holy cow... I'm supposed to do something else, but this paper looks amazing! I mean the concept, the implications... wow.
www.cell.com/cell/fulltex...
Norepinephrine-mediated slow vasomotion drives glymphatic clearance during sleep
Norepinephrine oscillations during NREM sleep drive synchronized changes in cerebral blood volume and cerebrospinal fluid, promoting glymphatic clearance. Optogenetic and pharmacological manipulations confirm that vasomotion, regulated by norepinephrine, acts as a pump for brain fluid transport.
www.cell.com
January 9, 2025 at 1:06 PM
🔵 #LocusCoeruleus alert!
Holy cow... I'm supposed to do something else, but this paper looks amazing! I mean the concept, the implications... wow.
www.cell.com/cell/fulltex...
Holy cow... I'm supposed to do something else, but this paper looks amazing! I mean the concept, the implications... wow.
www.cell.com/cell/fulltex...
Reposted by Johannes Bohacek
Join us on Feb 17 in Bern to celebrate the outstanding contributions of Prof. Jim McGaugh, winner of GSRNet Award 2025!
Exciting program: talks by Puder, Adamantidis, Tzanoulinou, Ruff, Andreas Luthi & roundtable with @bohaceklab.bsky.social B Kleim & von Känel
www.stressnetwork.ch/events/7th-a...
Exciting program: talks by Puder, Adamantidis, Tzanoulinou, Ruff, Andreas Luthi & roundtable with @bohaceklab.bsky.social B Kleim & von Känel
www.stressnetwork.ch/events/7th-a...
January 9, 2025 at 8:57 AM
Join us on Feb 17 in Bern to celebrate the outstanding contributions of Prof. Jim McGaugh, winner of GSRNet Award 2025!
Exciting program: talks by Puder, Adamantidis, Tzanoulinou, Ruff, Andreas Luthi & roundtable with @bohaceklab.bsky.social B Kleim & von Känel
www.stressnetwork.ch/events/7th-a...
Exciting program: talks by Puder, Adamantidis, Tzanoulinou, Ruff, Andreas Luthi & roundtable with @bohaceklab.bsky.social B Kleim & von Känel
www.stressnetwork.ch/events/7th-a...
Reposted by Johannes Bohacek
What a year! #DLC3🔥 is up tested by many of you (👀 watch out for a paper in 2025), we hit 750K downloads, and have over 120 contributors to the code! @deeplabcut.bsky.social
❄️⛷just dropped: new human pose models & high perf. models!
❄️⛷just dropped: new human pose models & high perf. models!
December 20, 2024 at 10:08 PM
What a year! #DLC3🔥 is up tested by many of you (👀 watch out for a paper in 2025), we hit 750K downloads, and have over 120 contributors to the code! @deeplabcut.bsky.social
❄️⛷just dropped: new human pose models & high perf. models!
❄️⛷just dropped: new human pose models & high perf. models!
Reposted by Johannes Bohacek
Scared to share this after 10 freaking years of waiting, but here we go: We found that temperature *perception* can change the biology of the next generations, even when “it’s just in the parents' head”. Read how C. elegans neurons control epigenetic inheritance!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Perception of Temperature Even in the Absence of Actual Change is Sufficient to Drive Transgenerational Epigenetic Inheritance
Can processes occurring in one individuals nervous system influence the physiology of the descendants? Here we explored the provocative hypothesis that parents sensation or perception of environmental...
www.biorxiv.org
December 5, 2024 at 7:32 PM
Scared to share this after 10 freaking years of waiting, but here we go: We found that temperature *perception* can change the biology of the next generations, even when “it’s just in the parents' head”. Read how C. elegans neurons control epigenetic inheritance!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...