Peter Rupprecht
@ptrrupprecht.bsky.social
Junior group leader in neuroscience - calcium imaging, ephys, plasticity, microscopy, neurons & astrocytes, data analysis. PhD with Rainer Friedrich, postdoc with Fritjof Helmchen. https://www.gcamp6f.com/
If you don't learn how to write, then you don't learn how to think.
November 15, 2025 at 3:52 PM
If you don't learn how to write, then you don't learn how to think.
Looks like a 1:1 copy of the Cambridge Technology 8 kHz scanner. Did you notice any differences? Or have they simply licensed the technology from the existing scanner?
October 22, 2025 at 7:58 PM
Looks like a 1:1 copy of the Cambridge Technology 8 kHz scanner. Did you notice any differences? Or have they simply licensed the technology from the existing scanner?
It's a great paper - congrats!
October 3, 2025 at 8:25 PM
It's a great paper - congrats!
This objective was designed for 2P imaging. For 1P imaging, I believe that there are still some technical problems to be solved. Perhaps @voigtvision.bsky.social or @nvladimus.bsky.social can tell you more!
September 29, 2025 at 9:15 AM
This objective was designed for 2P imaging. For 1P imaging, I believe that there are still some technical problems to be solved. Perhaps @voigtvision.bsky.social or @nvladimus.bsky.social can tell you more!
I'm using it mostly for 2P imaging, but it should also be relevant for expansion microscopy: Olympus XLPLN10XSVMP with NA 0.6, WD 8 mm: evidentscientific.com/en/objective...
Objective Finder | Evident Scientific | Olympus
Select the right lens for your application from our broad line of Olympus microscope objectives.
evidentscientific.com
September 28, 2025 at 6:22 PM
I'm using it mostly for 2P imaging, but it should also be relevant for expansion microscopy: Olympus XLPLN10XSVMP with NA 0.6, WD 8 mm: evidentscientific.com/en/objective...
Reflective Schmidt objectives with high NA and long WD: www.nature.com/articles/s41...
@voigtvision.bsky.social
@voigtvision.bsky.social
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope - Nature Biotechnology
Imaging of large, cleared samples in diverse media is achieved using a mirror objective.
www.nature.com
September 28, 2025 at 6:19 PM
Reflective Schmidt objectives with high NA and long WD: www.nature.com/articles/s41...
@voigtvision.bsky.social
@voigtvision.bsky.social
I also wonder whether you have observed any signs of cytotoxicity or fluorophore aggregates?
July 31, 2025 at 8:06 PM
I also wonder whether you have observed any signs of cytotoxicity or fluorophore aggregates?
This OCaMP sensor looks quite promising! Looking forward to trying it out!
July 31, 2025 at 8:01 PM
This OCaMP sensor looks quite promising! Looking forward to trying it out!
Thanks, it looks very interesting!
The first comment of reviewer #1 is also "interesting", asking for the "connectome" of VIP neurons for the revised manuscript ;-)
The first comment of reviewer #1 is also "interesting", asking for the "connectome" of VIP neurons for the revised manuscript ;-)
July 3, 2025 at 7:17 PM
Thanks, it looks very interesting!
The first comment of reviewer #1 is also "interesting", asking for the "connectome" of VIP neurons for the revised manuscript ;-)
The first comment of reviewer #1 is also "interesting", asking for the "connectome" of VIP neurons for the revised manuscript ;-)
Yes, this is great work! An important aspect is that it clearing of *archival* FFPE tissues over 10 years old. This method suddenly makes such biomedical archives much more valuable.
May 27, 2025 at 11:23 AM
Yes, this is great work! An important aspect is that it clearing of *archival* FFPE tissues over 10 years old. This method suddenly makes such biomedical archives much more valuable.
Could it also be feedback inhibition?
May 21, 2025 at 3:46 PM
Could it also be feedback inhibition?