Peter Rupprecht
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ptrrupprecht.bsky.social
Peter Rupprecht
@ptrrupprecht.bsky.social
Junior group leader in neuroscience - calcium imaging, ephys, plasticity, microscopy, neurons & astrocytes, data analysis. PhD with Rainer Friedrich, postdoc with Fritjof Helmchen. https://www.gcamp6f.com/
Great dataset! I was browsing through it (while looking for astrocytes), and wondered what this EM structure is: webknossos.brain.mpg.de/datasets/MCM...
Do you have an idea?
April 25, 2025 at 8:43 AM
Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1...

- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth

With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
March 11, 2025 at 11:30 AM
How to guide a micropipette into mouse hippocampus using two-photon microscopy - great protocol paper by Kevin Gonzalez and the Losonczy Lab: www.nature.com/articles/s41...
February 13, 2025 at 10:52 PM
Cool experimental design by @simwieg.bsky.social's lab with optogenetics + calcium imaging: how place cells in CA1 are affected by stimulation of contralateral CA3. They observe less an induction of place fields but somewhat random shifts of existing place fields: www.biorxiv.org/content/10.1...
January 31, 2025 at 11:01 PM
If you need any input with Cascade, just let me know! I was doing a lot more analysis on what to do with GCaMP8, will probably drop in 1 month or so. It also includes a scheme heavily inspired by Fig. 1 in your 2014 review ;-)
January 8, 2025 at 12:19 PM
Me!
Not a picture of a 2p setup, but some 2p images (of hippocampal astrocytes).
December 3, 2024 at 10:19 PM