Sofi Origanti
@proforiganti.bsky.social
Lab loves mechanisms-regulation: ribosomes, translation, DNA repair, cell cycle, cancer signaling & dogs! Uplift! opinions-own
Reposted by Sofi Origanti
Updated guidance on NIH grant application due dates (10/1/2025-12/5/2025), along with some other Info. grants.nih.gov/grants/guide...
NOT-OD-26-012: Updated Guidance on Reopening of NIH Extramural Activities Following the October 1, 2025 - Lapse in Appropriations
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Updated Guidance on Reopening of NIH Extramural Activities Following the October 1, 2025 - Lapse in Appropriations NOT-...
grants.nih.gov
November 24, 2025 at 5:54 PM
Updated guidance on NIH grant application due dates (10/1/2025-12/5/2025), along with some other Info. grants.nih.gov/grants/guide...
Reposted by Sofi Origanti
POSTDOCTORAL POSITION IN STRUCTURAL BIOLOGY
We need a structural biologist to work on several CryoEM datasets. For more information, please visit: www.antonylab.org
Expertise in structure refinement and model-building are required.
Please share.
We need a structural biologist to work on several CryoEM datasets. For more information, please visit: www.antonylab.org
Expertise in structure refinement and model-building are required.
Please share.
ANTONY LAB SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE
Edwin Antony Lab Website
www.antonylab.org
November 18, 2025 at 7:05 PM
POSTDOCTORAL POSITION IN STRUCTURAL BIOLOGY
We need a structural biologist to work on several CryoEM datasets. For more information, please visit: www.antonylab.org
Expertise in structure refinement and model-building are required.
Please share.
We need a structural biologist to work on several CryoEM datasets. For more information, please visit: www.antonylab.org
Expertise in structure refinement and model-building are required.
Please share.
Reposted by Sofi Origanti
Science People: We at NSF are still recovering/catching up/getting our lives together. But the agency posted these FAQs about post-shutdown resumption of operations which might answer a lot of Qs for you: www.nsf.gov/resumption-o...
Resumption of Operations at NSF
Information for NSF staff and the research community regarding the agency's resumption of operations after a lapse in appropriations.
www.nsf.gov
November 18, 2025 at 6:08 PM
Science People: We at NSF are still recovering/catching up/getting our lives together. But the agency posted these FAQs about post-shutdown resumption of operations which might answer a lot of Qs for you: www.nsf.gov/resumption-o...
Reposted by Sofi Origanti
We have written two reviews providing a practical guide and fundamentals of cell migration:
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
November 8, 2025 at 6:17 PM
We have written two reviews providing a practical guide and fundamentals of cell migration:
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
Reposted by Sofi Origanti
Want to know how homologous recombination defects are caused upon loss of BRCA2? Check out our recent efforts in uncovering this phenomenon just out in Science!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
FIGNL1 inhibits homologous recombination in BRCA2 deficient cells by dissociating RAD51 filaments
Homologous recombination (HR) deficiency upon Breast Cancer Gene 2 (BRCA2) loss arises from defects in the formation of RAD51 nucleoprotein filaments. We demonstrate that loss of the anti-recombinase ...
www.science.org
October 30, 2025 at 9:52 PM
Want to know how homologous recombination defects are caused upon loss of BRCA2? Check out our recent efforts in uncovering this phenomenon just out in Science!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sofi Origanti
Among the anti-recombinases, FIGNL1 rules them all. So much that inactivating it brings BRCA2-deficient cells to life. Who is responsible for RAD51 loading without BRCA2/FIGNL1, check out the paper to find out! Great collaboration with @raychaudhurilab.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
October 30, 2025 at 8:41 PM
Among the anti-recombinases, FIGNL1 rules them all. So much that inactivating it brings BRCA2-deficient cells to life. Who is responsible for RAD51 loading without BRCA2/FIGNL1, check out the paper to find out! Great collaboration with @raychaudhurilab.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sofi Origanti
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
October 24, 2025 at 5:44 PM
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
Reposted by Sofi Origanti
See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 27, 2025 at 1:12 PM
See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sofi Origanti
Beautiful reconstitution of amino acid stress-dependent ISR activation by my @harvardcellbio.bsky.social colleagues presenting a unifying mechanism for GCN2 activation, which requires ribosome collisions and is enhanced by cognate uncharged tRNA in the A site! www.science.org/doi/10.1126/...
GCN1 couples GCN2 to ribosomal state to initiate amino acid response pathway signaling
During nutrient deprivation, activation of the protein kinase GCN2 regulates cell survival and metabolic homeostasis. In addition to amino acid stress, GCN2 is activated by a variety of cellular stres...
www.science.org
October 3, 2025 at 11:02 AM
Beautiful reconstitution of amino acid stress-dependent ISR activation by my @harvardcellbio.bsky.social colleagues presenting a unifying mechanism for GCN2 activation, which requires ribosome collisions and is enhanced by cognate uncharged tRNA in the A site! www.science.org/doi/10.1126/...
Reposted by Sofi Origanti
Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science!
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...
Principles of cotranslational mitochondrial protein import
Selective ribosome profiling reveals that nearly 20% of mitochondrial proteins in
human cells are imported during translation on cytosolic ribosomes. Cotranslational
import requires an N-terminal pres...
www.cell.com
August 22, 2025 at 3:00 AM
Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science!
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...
Reposted by Sofi Origanti
The American Chemical Society announced that the ACS Board of Directors has approved $2.5 million to be granted to graduate students who have been impacted by the termination or cancellation of their adviser’s research grant(s) cen.acs.org/acs-news/acs... #chemsky
ACS announces millions in funding for students affected by terminated grants
The grants will help students within 1 year of completion of their degree
cen.acs.org
August 19, 2025 at 6:50 PM
The American Chemical Society announced that the ACS Board of Directors has approved $2.5 million to be granted to graduate students who have been impacted by the termination or cancellation of their adviser’s research grant(s) cen.acs.org/acs-news/acs... #chemsky
Reposted by Sofi Origanti
🧪BREAKING: NIH has laid out 5 options for funding publications costs on grants (yes, FIVE!). This is hugely impactful.
Make your opinions known by September 15, fellow scientists.
🧪
grants.nih.gov/grants/guide...
Make your opinions known by September 15, fellow scientists.
🧪
grants.nih.gov/grants/guide...
NOT-OD-25-138: Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs NOT-OD-25-138. NIH
grants.nih.gov
July 30, 2025 at 6:30 PM
🧪BREAKING: NIH has laid out 5 options for funding publications costs on grants (yes, FIVE!). This is hugely impactful.
Make your opinions known by September 15, fellow scientists.
🧪
grants.nih.gov/grants/guide...
Make your opinions known by September 15, fellow scientists.
🧪
grants.nih.gov/grants/guide...
Reposted by Sofi Origanti
Please register early and upload your abstracts. Looking forward to seeing many of you in Milwaukee in September!
The 45th Midwest Enzyme Chemistry Conference is ~9 weeks away!
Saturday, September 27, 2025 at University of Wisconsin-Milwaukee
mecc2025.midwestenzyme.org
Register/submit an abstract here: mecc2025.midwestenzyme.org/abstracts
Talks are selected from submitted abstracts.
Abstracts due: August 25
Saturday, September 27, 2025 at University of Wisconsin-Milwaukee
mecc2025.midwestenzyme.org
Register/submit an abstract here: mecc2025.midwestenzyme.org/abstracts
Talks are selected from submitted abstracts.
Abstracts due: August 25
Midwest Enzyme Chemistry Conference 2025
The 45th Midwest Enzyme Chemistry Conference
27th September 2025
University of Wisconsin-Milwaukee
Milwaukee, Wisconsin
mecc2025.midwestenzyme.org
July 24, 2025 at 2:52 PM
Please register early and upload your abstracts. Looking forward to seeing many of you in Milwaukee in September!
Reposted by Sofi Origanti
So good that NIGMS is planning to renew call for EI (and ESI) MIRA.
But really bad if this is just one deadline a year instead of the usual two (Jan and May)
Anyone know anything else? Maybe time to panic....
simpler.grants.gov/opportunity/...
But really bad if this is just one deadline a year instead of the usual two (Jan and May)
Anyone know anything else? Maybe time to panic....
simpler.grants.gov/opportunity/...
Opportunity Listing - Maximizing Investigators' Research Award
Read detailed information about this funding opportunity.
simpler.grants.gov
July 23, 2025 at 6:22 PM
So good that NIGMS is planning to renew call for EI (and ESI) MIRA.
But really bad if this is just one deadline a year instead of the usual two (Jan and May)
Anyone know anything else? Maybe time to panic....
simpler.grants.gov/opportunity/...
But really bad if this is just one deadline a year instead of the usual two (Jan and May)
Anyone know anything else? Maybe time to panic....
simpler.grants.gov/opportunity/...
up to 4th percentile for NCI 😳
Funding Policy - Research Grants
NCI funding policy for research project grant (RPG) awards reflects the funding goals of the institute, NIH, and HHS.
www.cancer.gov
July 23, 2025 at 6:14 PM
up to 4th percentile for NCI 😳
Reposted by Sofi Origanti
New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Global inhibition of deadenylation stabilizes the transcriptome in mitotic cells
In the presence of cell division errors, mammalian cells can pause in mitosis for tens of hours with little to no transcription, while still requiring continued translation for viability. These unique...
www.biorxiv.org
July 23, 2025 at 10:57 AM
New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sofi Origanti
How are Rad51 filaments formed during Homologous Recombination?
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination
Nature Communications - The mediator protein Rad52 promotes Rad51 binding onto RPA-coated DNA to initiate homologous recombination. Here, the authors show that Rad52 sorts Rad51 into monomers and...
urldefense.com
July 21, 2025 at 12:34 PM
How are Rad51 filaments formed during Homologous Recombination?
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.
urldefense.com/v3/__https:/...
Reposted by Sofi Origanti
NIH BREAKING NEWS NIH BREAKING NEWS
Hearing from multiple sources that an email went out late yesterday afternoon
Effective immediately: Please do not terminate any additional grant projects. Please pull back all grant projects that are in the cue to be terminated.
Hearing from multiple sources that an email went out late yesterday afternoon
Effective immediately: Please do not terminate any additional grant projects. Please pull back all grant projects that are in the cue to be terminated.
two men in suits and ties are waving their hands in a room
ALT: two men in suits and ties are waving their hands in a room
media.tenor.com
June 25, 2025 at 3:32 PM
NIH BREAKING NEWS NIH BREAKING NEWS
Hearing from multiple sources that an email went out late yesterday afternoon
Effective immediately: Please do not terminate any additional grant projects. Please pull back all grant projects that are in the cue to be terminated.
Hearing from multiple sources that an email went out late yesterday afternoon
Effective immediately: Please do not terminate any additional grant projects. Please pull back all grant projects that are in the cue to be terminated.
Reposted by Sofi Origanti
What? Some good news on a Friday?
Parent Fellowship Notices of Funding Opportunities are up!
F30, F31, F32, F33
grants.nih.gov/grants/guide...
Parent Fellowship Notices of Funding Opportunities are up!
F30, F31, F32, F33
grants.nih.gov/grants/guide...
PA-25-422: Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship (Parent F31)
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship (Parent F31) PA-25-422. NI...
grants.nih.gov
June 13, 2025 at 11:06 PM
What? Some good news on a Friday?
Parent Fellowship Notices of Funding Opportunities are up!
F30, F31, F32, F33
grants.nih.gov/grants/guide...
Parent Fellowship Notices of Funding Opportunities are up!
F30, F31, F32, F33
grants.nih.gov/grants/guide...
Reposted by Sofi Origanti
Registration now open for the Midwest Enzyme Chemistry Conference 2025.
Registration Link: lnkd.in/ggHMWrF5
Event Date: September 27, 2025
Location: University of Wisconsin - Milwaukee
It's free to register
Kindly share as we want to grow this amazing community of mechanistic enzymologists.....
Registration Link: lnkd.in/ggHMWrF5
Event Date: September 27, 2025
Location: University of Wisconsin - Milwaukee
It's free to register
Kindly share as we want to grow this amazing community of mechanistic enzymologists.....
May 28, 2025 at 6:20 PM
Registration now open for the Midwest Enzyme Chemistry Conference 2025.
Registration Link: lnkd.in/ggHMWrF5
Event Date: September 27, 2025
Location: University of Wisconsin - Milwaukee
It's free to register
Kindly share as we want to grow this amazing community of mechanistic enzymologists.....
Registration Link: lnkd.in/ggHMWrF5
Event Date: September 27, 2025
Location: University of Wisconsin - Milwaukee
It's free to register
Kindly share as we want to grow this amazing community of mechanistic enzymologists.....
Reposted by Sofi Origanti
New NIH Notice rescinding the no DEI, no Israeli boycott term and condition added to NIH grants (covers the whole university)
This is what Williams refused to sign and declined all federal funding.
grants.nih.gov/grants/guide...
1/2
This is what Williams refused to sign and declined all federal funding.
grants.nih.gov/grants/guide...
1/2
NOT-OD-25-124: Notice of Rescission of Civil Rights Term and Condition of Award
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Notice of Rescission of Civil Rights Term and Condition of Award NOT-OD-25-124. NIH
grants.nih.gov
June 9, 2025 at 2:36 PM
New NIH Notice rescinding the no DEI, no Israeli boycott term and condition added to NIH grants (covers the whole university)
This is what Williams refused to sign and declined all federal funding.
grants.nih.gov/grants/guide...
1/2
This is what Williams refused to sign and declined all federal funding.
grants.nih.gov/grants/guide...
1/2
Reposted by Sofi Origanti
Wow, 7% depressing. In other news, please check your compiled grants in ERA Commons. 129 pages of our P50 missing going between the final preview of submitted Grant in ASSIST to the compiled grant in Commons. No errors. Put in ticket. Fixed. Still 2 pages missing. The NIH is not alright. 🙁
NCI paylines drop to 7%ile for established investigator applications. It’s at 9%ile for Early Stage Investigators.
www.cancer.gov/grants-train...
www.cancer.gov/grants-train...
Funding Policy - Research Grants
NCI funding policy for research project grant (RPG) awards reflects the funding goals of the institute, NIH, and HHS.
www.cancer.gov
May 23, 2025 at 3:45 PM
Wow, 7% depressing. In other news, please check your compiled grants in ERA Commons. 129 pages of our P50 missing going between the final preview of submitted Grant in ASSIST to the compiled grant in Commons. No errors. Put in ticket. Fixed. Still 2 pages missing. The NIH is not alright. 🙁
Reposted by Sofi Origanti
🚨 A new rule would let career scientists like NSF/NIH program officers be replaced by political appointees
Already 14,000+ public comments, deadline is Friday
📣 Comments can be short. Courts consider them—and scientists with NSF/NIH experience are especially impactful
Speak up! shorturl.at/WKuBj
Already 14,000+ public comments, deadline is Friday
📣 Comments can be short. Courts consider them—and scientists with NSF/NIH experience are especially impactful
Speak up! shorturl.at/WKuBj
May 21, 2025 at 5:39 PM
🚨 A new rule would let career scientists like NSF/NIH program officers be replaced by political appointees
Already 14,000+ public comments, deadline is Friday
📣 Comments can be short. Courts consider them—and scientists with NSF/NIH experience are especially impactful
Speak up! shorturl.at/WKuBj
Already 14,000+ public comments, deadline is Friday
📣 Comments can be short. Courts consider them—and scientists with NSF/NIH experience are especially impactful
Speak up! shorturl.at/WKuBj
Reposted by Sofi Origanti
poor nutrient-induced ribosomal frameshift promotes mRNA decay in eukaryotes and bacteria- from @pelechanolab.bsky.social #RNASky #ribosome 🧪
Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay
Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay
Zhang et al. identify a regulatory mechanism for how cells adapt to nutrient scarcity through widespread −1 ribosomal frameshifts, culminating in accelerated mRNA decay. This process, dependent on codon optimality and conserved across species, establishes direct feedback coupling the translation of new proteins with the stability of the mRNA that encodes for them.
dlvr.it
May 18, 2025 at 2:11 PM
poor nutrient-induced ribosomal frameshift promotes mRNA decay in eukaryotes and bacteria- from @pelechanolab.bsky.social #RNASky #ribosome 🧪
Reposted by Sofi Origanti
Our study highlights RNA as a direct template for DNA repair, revealing RT-DSBR as a previously unrecognized and potentially mutagenic pathway. Huge thanks to the incredible team who made this work possible! (5/5)
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
RNA transcripts serve as a template for double-strand break repair in human cells - Nature Communications
DNA double-stranded breaks threaten genome stability. Here, the authors show that transcript RNA serves as a repair template in human cells and identify Polζ as a key factor in RNA-templated DSB repai...
www.nature.com
May 12, 2025 at 5:49 PM
Our study highlights RNA as a direct template for DNA repair, revealing RT-DSBR as a previously unrecognized and potentially mutagenic pathway. Huge thanks to the incredible team who made this work possible! (5/5)
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB