Nikos Kouvelas
@nickkouvelas.bsky.social
RNA-curious PhD student | Mühlemann Lab | NCCR_RNADisease
Reposted by Nikos Kouvelas
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal peptidyl transferase inhibitor
Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...
www.biorxiv.org
October 13, 2025 at 10:35 AM
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
Reposted by Nikos Kouvelas
Very harmonious and inspiring collaboration with Niels Gehring, @volkerboehm.bsky.social and team at Uni Köln, and colleagues at @mdc-berlin.bsky.social . Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome: Molecular Cell www.cell.com/molecular-ce...
Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome
UPF1 depletion inhibits nonsense-mediated mRNA decay (NMD) and stabilizes direct target
transcripts within hours. Boehm et al. use conditional protein degradation and advanced
transcriptomics to map t...
www.cell.com
September 12, 2025 at 7:26 AM
Very harmonious and inspiring collaboration with Niels Gehring, @volkerboehm.bsky.social and team at Uni Köln, and colleagues at @mdc-berlin.bsky.social . Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome: Molecular Cell www.cell.com/molecular-ce...
This is a step-by-step guide on how to produce translation-competent lysates from human cells using dual centrifugation, as well as how to assess mRNA translation and degradation. #RNA #RNASky
Protocol for monitoring mRNA translation and degradation in human cell-free lysates
Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation u…
www.sciencedirect.com
September 12, 2025 at 6:05 AM
Reposted by Nikos Kouvelas
Final call for postdoc positions in Aarhus, Denmark. Sorting of gene expression and beyond. Please get in touch for further information or apply using this link:
mbg.au.dk/en/news-and-...
mbg.au.dk/en/news-and-...
A postdoc position in the Torben Heick Jensen lab, Aarhus University, Denmark: Mammalian Nuclear RNA Production and Turnover Systems - Vacancy at Aarhus University
Vacancy at Department of Molecular Biology and Genetics - RNA Biology and Innovation, Aarhus University
mbg.au.dk
August 5, 2025 at 8:06 AM
Final call for postdoc positions in Aarhus, Denmark. Sorting of gene expression and beyond. Please get in touch for further information or apply using this link:
mbg.au.dk/en/news-and-...
mbg.au.dk/en/news-and-...
Reposted by Nikos Kouvelas
Postdoc positions available in the THJ group in Aarhus, Denmark 🇩🇰
Please share around 🤝
Please share around 🤝
Postdoc positions available in the Heick Jensen lab in sunny Aarhus. Sorting of good and bad RNAs in mammalian nuclei. Please get in touch for further information and/or use the following link to apply:
mbg.au.dk/en/news-and-...
mbg.au.dk/en/news-and-...
A postdoc position in the Torben Heick Jensen lab, Aarhus University, Denmark: Mammalian Nuclear RNA Production and Turnover Systems - Vacancy at Aarhus University
Vacancy at Department of Molecular Biology and Genetics - RNA Biology and Innovation, Aarhus University
mbg.au.dk
July 22, 2025 at 6:07 AM
Postdoc positions available in the THJ group in Aarhus, Denmark 🇩🇰
Please share around 🤝
Please share around 🤝
Reposted by Nikos Kouvelas
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay - Nature Communications
Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...
www.nature.com
July 13, 2025 at 3:59 AM
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
Reposted by Nikos Kouvelas
"m6A in the coding sequence: linking deposition, translation and decay"
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
July 8, 2025 at 12:07 PM
"m6A in the coding sequence: linking deposition, translation and decay"
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
Reposted by Nikos Kouvelas
Reposted by Nikos Kouvelas
🚨 We’re looking for a curious mind to join our lab at the NIH!
Passionate about #RNA biology, #MachineLearning, and the #genomics of #aging?
Interested in a postdoc or a PhD research visit at NIH? Let’s connect.
📩 DM or email for details!
#Bioinformatics #AI #ComputationalBiology
Passionate about #RNA biology, #MachineLearning, and the #genomics of #aging?
Interested in a postdoc or a PhD research visit at NIH? Let’s connect.
📩 DM or email for details!
#Bioinformatics #AI #ComputationalBiology
June 4, 2025 at 5:30 PM
🚨 We’re looking for a curious mind to join our lab at the NIH!
Passionate about #RNA biology, #MachineLearning, and the #genomics of #aging?
Interested in a postdoc or a PhD research visit at NIH? Let’s connect.
📩 DM or email for details!
#Bioinformatics #AI #ComputationalBiology
Passionate about #RNA biology, #MachineLearning, and the #genomics of #aging?
Interested in a postdoc or a PhD research visit at NIH? Let’s connect.
📩 DM or email for details!
#Bioinformatics #AI #ComputationalBiology
Easy, rapid and highly reproducible generation of translation-competent, cell-type-specific lysates from different human cells. #RNA #RNASky
Efficient cell-free translation from diverse human cell types
Cell-free translation systems are indispensable for studying protein synthesis, enabling
researchers to explore translational regulation across different cell types. The difficulties
in producing cell...
www.jbc.org
June 2, 2025 at 6:42 AM
Reposted by Nikos Kouvelas
Sad that #RNA2025 is almost over? Join us in August in the UK for another fun meeting on RNA! #RNAsky #RNAdecay
Please join us for an exciting meeting on "Eukaryotic RNA turnover: from fundamentals to applications" in Brighton, UK this August! We have an exciting lineup of speakers, and will feature many selected talks. Abstract deadline is June 27! www.eventsforce.net/biochemsoc/f...
www.eventsforce.net
May 31, 2025 at 4:01 PM
Reposted by Nikos Kouvelas
Identified in the 1970s, the precise molecular function of the alternative initiation factor eIF2A has remained unclear. Here, we map its interactome and uncover a surprising role in ribosome-associated quality control (RQC) upon ribosome stalling. 1/x
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The alternative initiation factor eIF2A regulates 40S subunit turnover in ribosome-associated quality control
The noncanonical translation initiation factor eIF2A plays critical roles in diverse cellular processes, including the integrated stress response, neurodegeneration and tumorigenesis. However, the pre...
www.biorxiv.org
May 26, 2025 at 9:35 PM
Identified in the 1970s, the precise molecular function of the alternative initiation factor eIF2A has remained unclear. Here, we map its interactome and uncover a surprising role in ribosome-associated quality control (RQC) upon ribosome stalling. 1/x
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
New study confirms m6A-driven mRNA decay and reveals a key role for tRNA's mcm5s2U in regulating this translation-linked pathway. #RNA #RNASky
tRNA modifications tune m6A-dependent mRNA decay
Chemically modified nucleotides in mRNA are critical regulators of gene expression, primarily through interactions with reader proteins that bind to t…
www.sciencedirect.com
May 2, 2025 at 11:29 AM
Reposted by Nikos Kouvelas
Please join us for an exciting meeting on "Eukaryotic RNA turnover: from fundamentals to applications" in Brighton, UK this August! We have an exciting lineup of speakers, and will feature many selected talks. Abstract deadline is June 27! www.eventsforce.net/biochemsoc/f...
www.eventsforce.net
April 25, 2025 at 2:37 PM
Please join us for an exciting meeting on "Eukaryotic RNA turnover: from fundamentals to applications" in Brighton, UK this August! We have an exciting lineup of speakers, and will feature many selected talks. Abstract deadline is June 27! www.eventsforce.net/biochemsoc/f...
Reposted by Nikos Kouvelas
We are looking for a postdoctoral fellow to join us to explore telomerase regulation by histones. Please get in touch if this may interest you.
Excellent opportunity available for a motivated #postdoc to join @kellythd-nguyen.bsky.social’s group investigating the roles of histones in regulating telomerase using a multi-disciplinary approach.
More details: www.nature.com/naturecareer...
Apply by 04 MAY
#PostdocJobs #ScienceJobs
More details: www.nature.com/naturecareer...
Apply by 04 MAY
#PostdocJobs #ScienceJobs
April 16, 2025 at 5:25 PM
We are looking for a postdoctoral fellow to join us to explore telomerase regulation by histones. Please get in touch if this may interest you.
Reposted by Nikos Kouvelas
Check out our preprint at BioRxiv revealing mechanisms of mRNP assembly during translation repression: (www.biorxiv.org/content/10.1...). Great collaboration with Olivier Duss (@olivierduss.bsky.social). Great Work Marco Payr (@marcopayr.bsky.social) and thanks for the video, explaining our paper.
April 11, 2025 at 4:35 AM
Check out our preprint at BioRxiv revealing mechanisms of mRNP assembly during translation repression: (www.biorxiv.org/content/10.1...). Great collaboration with Olivier Duss (@olivierduss.bsky.social). Great Work Marco Payr (@marcopayr.bsky.social) and thanks for the video, explaining our paper.
Reposted by Nikos Kouvelas
I've built an #RNA feed for personal use to capture RNA biology posts across the entire network. Some arbitrary filters used to hopefully increase signal to noise ratio but otherwise not moderated. Feel free to follow if you find useful.
April 10, 2025 at 4:09 PM
I've built an #RNA feed for personal use to capture RNA biology posts across the entire network. Some arbitrary filters used to hopefully increase signal to noise ratio but otherwise not moderated. Feel free to follow if you find useful.
Reposted by Nikos Kouvelas
Our latest research on #Coronavirus Nsp1 found its home (and a new name) at @cp-cellreports.bsky.social www.cell.com/cell-reports... Thankful to the first (dream) team of my lab and funding for making this happen. @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social @unibe.ch
The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition
Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition
and mRNA degradation using a human cell-free translation system. They demonstrate
that SARS-CoV-2 Nsp1 mediates mRNA ...
www.cell.com
March 28, 2025 at 5:00 PM
Our latest research on #Coronavirus Nsp1 found its home (and a new name) at @cp-cellreports.bsky.social www.cell.com/cell-reports... Thankful to the first (dream) team of my lab and funding for making this happen. @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social @unibe.ch